The effects of curcumin supplementation on oxidative stress, Sirtuin-1 and peroxisome proliferator activated receptor γ coactivator 1α gene expression in polycystic ovarian syndrome (PCOS) patients: A randomized placebo-controlled clinical trial

2020 ◽  
Vol 14 (2) ◽  
pp. 77-82 ◽  
Author(s):  
Javad Heshmati ◽  
Fereshteh Golab ◽  
Mojgan Morvaridzadeh ◽  
Eric Potter ◽  
Maryam Akbari-Fakhrabadi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Clinical Trial ◽  
Polycystic Ovarian Syndrome ◽  
Sirtuin 1 ◽  
Controlled Clinical Trial ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Ovarian Syndrome

scholarly journals Beta-sitosterol upregulated paraoxonase-1 via peroxisome proliferator-activated receptor-γ in irradiated rats

2017 ◽  
Vol 95 (6) ◽  
pp. 661-666 ◽  
Author(s):  
Enas Mahmoud Moustafa ◽  
Noura Magdy Thabet
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Enzyme Activities ◽  
Liver Tissue ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Oxidative Stress Marker ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Ppar Γ

This study was designed to evaluate the effect of beta-sitosterol (BS) on the peroxisome proliferator-activated receptor gamma (PPAR-γ) gene expression role in the activity of paraoxonase (PON-1) enzyme in oxidative stress status of irradiated rats. Animals were exposed to whole body γ-radiation single dose 6 Gy and received BS dose (40 mg·(kg body mass)−1·day−1, orally). In liver tissue, gene expression of PPAR-γ ligand was determined. Oxidative stress marker (malondialdehyde, MDA) and antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), PON-1, and arylesterase (ARE)) were assayed in serum and liver tissue. Also, serum lipid profile (cholesterol, triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c)) was measured. In irradiated animals that received BS, expression of PPAR-γ ligand increase significantly associated with increase in PON-1 and ARE enzyme activities. Also, the activities of SOD, CAT enzymes, and HDL-c levels display elevation. By contrast, significant decrease in MDA content, cholesterol, TG, and LDL-c levels were revealed after BS administration. Our findings in this study provide the evidence that BS has radio-protective effect via regulating the gene expression of PPAR-γ, causing an increase in PON-1 and ARE enzyme activities. This action of BS is due to its free radical scavenging properties, antioxidant effect, lowering of cholesterol, and PPAR-γ agonist properties.

Effect of Prebiotic on Anthropometric Indices in Women with Polycystic Ovarian Syndrome: A Triple-Blind, Randomized, Controlled Clinical Trial

2018 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Sevda Gholizadeh Shamasbi ◽  
Parvin Dehghan ◽  
Sakineh Mohammad-Alizadeh Charandabi ◽  
Akbar Aliasgarzadeh ◽  
Mojgan Mirghafourvand
Keyword(s):  
Clinical Trial ◽  
Polycystic Ovarian Syndrome ◽  
Controlled Clinical Trial ◽  
Randomized Controlled Clinical Trial ◽  
Anthropometric Indices ◽  
Randomized Controlled ◽  
Ovarian Syndrome

scholarly journals Curcumin Eliminates Leptin’s Effects on Hepatic Stellate Cell Activation via Interrupting Leptin Signaling

Endocrinology ◽  
2009 ◽  
Vol 150 (7) ◽  
pp. 3011-3020 ◽  
Author(s):  
Youcai Tang ◽  
Shizhong Zheng ◽  
Anping Chen
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Leptin Receptor ◽  
De Novo ◽  
Stellate Cell ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Leptin Signaling ◽  
Liver Fibrogenesis

Nonalcoholic steatohepatitis (NASH) is commonly found in patients with obesity and is often accompanied with abnormally elevated levels of plasma leptin, i.e. hyperleptinemia. A relatively high population of NASH patients develops hepatic fibrosis, even cirrhosis. Hepatic stellate cells (HSCs) are the major effector cells during liver fibrogenesis and could be activated by leptin. The antioxidant curcumin, a phytochemical from turmeric, has been shown to suppress HSC activation in vitro and in vivo. This project is to evaluate the effect of curcumin on leptin-induced HSC activation and to elucidate the underlying mechanisms. We hypothesize that curcumin abrogates the stimulatory effect of leptin on HSC activation by interrupting leptin signaling and attenuating leptin-induced oxidative stress. Curcumin eliminates the stimulatory effects of leptin on regulating expression of genes closely relevant to HSC activation. Curcumin interrupts leptin signaling by reducing phosphorylation levels of leptin receptor (Ob-R) and its downstream intermediators. In addition, curcumin suppresses gene expression of Ob-R in HSCs, which requires the activation of endogenous peroxisome proliferator-activated receptor-γ and de novo synthesis of glutathione. In conclusion, our results demonstrate that curcumin abrogates the stimulatory effect of leptin on HSC activation in vitro by reducing the phosphorylation level of Ob-R, stimulating peroxisome proliferator-activated receptor-γ activity, and attenuating oxidative stress, leading to the suppression of Ob-R gene expression and interruption of leptin signaling. These results provide novel insights into therapeutic mechanisms of curcumin in inhibiting HSC activation and intervening liver fibrogenesis associated with hyperleptinemia in NASH patients.

scholarly journals Randomized double blind clinical trial evaluating the Ellagic acid effects on insulin resistance, oxidative stress and sex hormones levels in women with polycystic ovarian syndrome

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Mahnaz Kazemi ◽  
Fatemeh Lalooha ◽  
Mohammadreza Rashidi Nooshabadi ◽  
Fariba Dashti ◽  
Maria Kavianpour ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Clinical Trial ◽  
Sex Hormones ◽  
Polycystic Ovarian Syndrome ◽  
Density Lipoprotein ◽  
Double Blind ◽  
Tnf Α ◽  
The Mean ◽  
Ovarian Syndrome

Abstract Objective The design of this study was due to the report of the antioxidant properties of Ellagic acid (EA) for its evaluation on the Insulin resistance (IR), oxidative stress and sex hormones levels in women with polycystic ovarian syndrome (PCOS). Methods In this randomized, double-blind, placebo-controlled clinical trial, 60 patients were recruited. Patients were randomly allocated consumed a capsule containing 200 mg of EA per day (n = 30) or placebo (n = 30) for 8 weeks. The fasting blood sugar (FBS), insulin, IR, total cholesterol (TC), triglycerides (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), total antioxidant capacity (TAC), Malondialdehyde (MDA), C-reactive protein (CRP), Tumor necrosis factor-alpha (TNF-α), sex hormones and anti-mullerian hormone (AMH) were measured at the beginning and end of the study. Result At the end of the study, the mean of FBS, insulin, IR, TC, TG, LDL, MDA, CRP, TNF-α, total testosterone, prolactin and AMH were significantly decreased in the intervention group compared to the placebo group (P < 0.05). Also, there was a significant increase in the mean of TAC after supplementation with EA (P < 0.05). At the end of the study, no significant changes were observed in the mean of anthropometric factors, physical activity and food intake (P > 0.05). Conclusion EA supplementation can be helpful as a diet supplement in women with PCOS through improvement in insulin resistance. This supplement may be used to reduce metabolic disorders in women. Trial registration This study was retrospectively (07–07-2019) registered in the Iranian website (www.irct.ir) for registration of clinical trials (IRCT20141025019669N12).

scholarly journals Effects of Ferula assa-foetida on clinical, hormonal and ultrasound parameters in young girls with polycystic ovary syndrome: a randomized, placebo controlled, triple-blinded

2020 ◽  
Author(s):  
Fatemeh Ghavi ◽  
somayeh abdolahian ◽  
Fatemeh Shakeri ◽  
Mahboubeh Taebi
Keyword(s):  
Clinical Trial ◽  
Polycystic Ovarian Syndrome ◽  
Endometrial Thickness ◽  
Intervention Group ◽  
Ovarian Follicles ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
P Value ◽  
Young Girls ◽  
Ovarian Syndrome

Abstract Background: Polycystic ovarian syndrome is the most common endocrine disorder in reproductive aged women. As a result of side effect of pharmaceutical medications women are interested in using alternative medicines to treat. To determine the comparative effects of Ferula assa-foetida on androgenic hormone levels and ovarian features in patient with polycystic ovarian syndrome (PCOS). Methods: In this triple-blinded controlled clinical trial, 34 student participants were randomly divided in two groups. Intervention group received 100 mg of oleo-gum resin of Ferula assa- foetida, control group received oral paraffin (Placebo) twice daily for 3 months. The efficacy of this herbal medicines was measured after the 3-month intervention. Hormonal assay for evaluating Testosterone, DEHAS, Prolactin, TSH, FSH, LH levels and also trans abdominal ultrasound for evaluating ovarian volumes, number of follicles of both ovaries, and endometrial thickness. Were measured before and after the study. Results: In this study, significant changes in DEHAS and TSH level were observed (p value<0.03). The greatest reduction in the number of ovarian follicles was reported in the Ferula group (p value <0.00). Conclusion: Use of Ferula assa-foetida can be effected in decrease of DHEAS, TSH levels, and ovarian follicles number in young girls with PCOS.Trial registration: the Iranian Randomized Clinical Trial (IRCT2016040427207N1).url: https://www.irct.ir/trial/22343

scholarly journals Bariatric surgery can acutely modulate ER-stress and inflammation on subcutaneous adipose tissue in non-diabetic patients with obesity

2020 ◽  
Author(s):  
Rafael Ferraz-Bannitz ◽  
Caroline Rossi Welendorf ◽  
Priscila Oliveira Coelho ◽  
Wilson Salgado ◽  
Carla Barbosa Nonino ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Bariatric Surgery ◽  
Weight Loss ◽  
Adipose Tissue ◽  
Energy Homeostasis ◽  
Diabetic Patients ◽  
Peroxisome Proliferator ◽  
Strong Positive Correlation ◽  
Peroxisome Proliferator Activated Receptor

Abstract Background Bariatric surgery, especially Roux-en-Y gastric bypass (RYGB) is the most effective and durable treatment option for population with severe obesity. The mechanisms involving adipose tissue may be important to explain the effects of surgery. Methods We aimed to identify the genetic signatures of adipose tissue in patients undergoing RYGB. We evaluated 13 obese, non-diabetic patients (mean age 37 years, 100% women, Body mass index (BMI) 42.2 kg/m2) one day before surgery, 3 and 6 months (M) after RYGB. Results Analysis of gene expression in adipose tissue collected at surgery compared with samples collected at 3M and 6M Post-RYGB showed that interleukins (Interleukin 6, Tumor necrosis factor-α (TNF-α), and Monocyte chemoattractant protein-1(MCP1)) and endoplasmic reticulum stress (ERS) genes (Eukaryotic translation initiation factor 2 alpha kinase 3 (EIF2AK3) and Calreticulin (CALR)) decreased during the follow-up (P ≤ 0.01 for all). Otherwise, genes involved in energy homeostasis (Adiponectin and AMP-activated protein kinase (AMPK)), cellular response to oxidative stress (Sirtuin 1, Sirtuin 3, and Nuclear factor erythroid 2-related factor 2 (NRF2)), mitochondrial biogenesis (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α)) and amino acids metabolism (General control nonderepressible 2 (GCN2)) increased from baseline to all other time points evaluated (P ≤ 0.01 for all). Also, expression of Peroxisome proliferator activated receptor gamma (PPARϒ) (adipogenesis regulation) was significantly decreased after RYGB (P < 0.05) We also observed a strong positive correlation between PGC1α, SIRT1 and AMPK with BMI at 3M (P ≤ 0.01 for all) and ADIPOQ and SIRT1 with BMI at 6M (P ≤ 0.01 for all). Conclusions Our findings demonstrate that weight loss is associated with amelioration of inflammation and ERS and increased protection against oxidative stress in adipose tissue. These observations are strongly correlated with a decrease in BMI and essential genes that control cellular energy homeostasis, suggesting an adaptive process on a gene expression level during the caloric restriction and weight loss period after RYGB.

Sign in / Sign up

Export Citation Format

Share Document