An off-on fluorescent probe for real-time sensing the fluctuations of intracellular pH values in biological processes

2019 ◽  
Vol 170 ◽  
pp. 107620 ◽  
Author(s):  
Jie Zhang ◽  
Jianguo Li ◽  
Bochao Chen ◽  
Jianfei Kan ◽  
Tiantian Jiang ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Biological Processes ◽  
Ph Values

Construction of Polysiloxane-Based Fluorescent Probe for Visualizing The pH Down-regulation

2021 ◽  
Author(s):  
Xiaoni Wang ◽  
Zhiming Gou ◽  
Minggang Tian ◽  
Yujing Zuo
Keyword(s):  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Cell Apoptosis ◽  
Pathological Process ◽  
Down Regulation ◽  
Ph Values

Intracellular pH values change is a significant physiological and pathological process and plays vital roles in autophagy, self-repairing, and programmed cell apoptosis. A unique fluorescent probe (PN-1) based on polysiloxanes...

A review: the trend of progress about pH probes in cell application in recent years

The Analyst ◽  
2017 ◽  
Vol 142 (1) ◽  
pp. 30-41 ◽  
Author(s):  
Yongkang Yue ◽  
Fangjun Huo ◽  
Songyi Lee ◽  
Caixia Yin ◽  
Juyoung Yoon
Keyword(s):  
Intracellular Ph ◽  
Body Fluids ◽  
The Body ◽  
Biological Processes ◽  
Acid Base ◽  
Ph Values ◽  
Ph Probes ◽  
In Cells

Intracellular pH values are some of the most important factors that govern biological processes and the acid–base homeostasis in cells, body fluids and organs sustains the normal operations of the body.

Precise aggregation-induced emission enhancement via H+ sensing and its use in ratiometric detection of intracellular pH values

RSC Advances ◽  
2014 ◽  
Vol 4 (103) ◽  
pp. 59078-59082 ◽  
Author(s):  
Anushri Rananaware ◽  
Rajesh S. Bhosale ◽  
Hemlata Patil ◽  
Mohammad Al Kobaisi ◽  
Amanda Abraham ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Organic Solvents ◽  
Fluorescent Detection ◽  
Aggregation Induced Emission ◽  
Ph Values ◽  
Emission Enhancement ◽  
Ratiometric Detection

A pyridyl functionalised tetraphenylethylene (Py-TPE) for ratiometric fluorescent detection of intracellular pH values is reported; the Py-TPE fluorescent probe can be used for H+ sensing in organic solvents (CHCl3, DMF and MeOH).

A novel nanogel-based fluorescent probe for ratiometric detection of intracellular pH values

2014 ◽  
Vol 50 (63) ◽  
pp. 8787-8790 ◽  
Author(s):  
Lixia Cao ◽  
Xiaoyan Li ◽  
Shuangqing Wang ◽  
Shayu Li ◽  
Yi Li ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Living Cells ◽  
Fluorescent Detection ◽  
Hypsochromic Shift ◽  
Ph Changes ◽  
Ph Values ◽  
Ratiometric Detection ◽  
Cytosol Ph

A new nanogel indicator (NGI) for ratiometric fluorescent detection of intracellular pH values has been reported. In aqueous solution, the NGI can be used for ratiometric sensing of pH changes with a large hypsochromic shift of 100 nm in the green–red region and reversible. Furthermore, the NGI has been used to calibrate the cytosol pH in living cells.

scholarly journals Quantitative real-time imaging of glutathione

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiqian Jiang ◽  
Jianwei Chen ◽  
Aleksandar Bajić ◽  
Chengwei Zhang ◽  
Xianzhou Song ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Confocal Microscopy ◽  
Real Time ◽  
Fluorescent Probe ◽  
High Throughput ◽  
Single Cells ◽  
Living Cells ◽  
Biological Processes ◽  
Dynamic Changes ◽  
Versatile Tool

AbstractGlutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown. Here, we report a reversible reaction-based fluorescent probe—designated as RealThiol (RT)—that can quantitatively monitor the real-time glutathione dynamics in living cells. Using RT, we observe enhanced antioxidant capability of activated neurons and dynamic glutathione changes during ferroptosis. RT is thus a versatile tool that can be used for both confocal microscopy and flow cytometry based high-throughput quantification of glutathione levels in single cells. We envision that this new glutathione probe will enable opportunities to study glutathione dynamics and transportation and expand our understanding of the physiological and pathological roles of glutathione in living cells.

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