scholarly journals A Wide Range of pH-Sensitive Fluorescent Probe based on Responsive Double Hydrophilic Block Copolymer for Real-Time Intracellular pH Sensing in Water Samples

2019 ◽  
Vol 562 ◽  
pp. 012053
Author(s):  
Zijuan Zhou
Keyword(s):  
Block Copolymer ◽  
Real Time ◽  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Water Samples ◽  
Ph Sensitive ◽  
Ph Sensing ◽  
Wide Range

A pH-sensitive multifunctional fluorescent probe based on N-annulated perylene for the sensitive and selective detection of hypochlorous acid

2017 ◽  
Vol 1 (11) ◽  
pp. 2292-2298 ◽  
Author(s):  
Xiao Zhang ◽  
Yongchao Yan ◽  
Qing Peng ◽  
Jian Wang ◽  
Yandi Hang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Water Samples ◽  
Hypochlorous Acid ◽  
Ph Sensitive ◽  
Selective Detection

The pH-sensitive multifunctional fluorescent probe PNPM based on N-annulated perylene was firstly developed for detecting HOCl selectively. And the probe could detect pH and HOCl simultaneously, and was successfully employed to detect HOCl in serum and water samples.

scholarly journals A Mini Review on pH-Sensitive Photoluminescence in Carbon Nanodots

2021 ◽  
Vol 8 ◽  
Author(s):  
Cui Liu ◽  
Fang Zhang ◽  
Jiao Hu ◽  
Wenhui Gao ◽  
Mingzhen Zhang
Keyword(s):  
Optical Properties ◽  
Low Cost ◽  
Ph Sensitive ◽  
Research Progress ◽  
Future Research ◽  
Carbon Nanodots ◽  
Ph Sensing ◽  
Light Emitting ◽  
Light Emitting Devices ◽  
Wide Range

Carbon nanodots (C-dots) with sp2/sp3 framework and diameter of <10 nm contain abundant functional groups or polymers on their surface. C-dots have attracted immense attention because of their unique optical properties, excellent biocompatibility, facile preparation, and low cost. With these merits, C-dots have been used in a wide range of applications including sensing, bioimaging, catalysis, and light-emitting devices. C-dots exhibit good optical properties, such as tunable emission wavelength, good photostability, nonblinking, up-conversion emission, etc. Of note, C-dots show intrinsic pH-sensitive photoluminescence (PL), indicating their great potential for pH sensing, especially in biotic pH sensing. In this review, we systematically summarize the pH-sensitive PL properties and the pH-sensitive PL mechanism, as well as recent research progress of C-dots in pH sensing. The current challenges of pH-sensitive C-dots and their future research focus are also proposed here. We anticipate this review might be of great significance for understanding the characteristics of pH-sensitive C-dots and the development of photoluminescent nanomaterials with pH-sensitive properties.

Evaluation of an integrated hydrocarbon sensor array system for the detection of dissolved oil components in sea water and its potential application in seepage exploration

2010 ◽  
Vol 50 (2) ◽  
pp. 724
Author(s):  
Xiubin Qi ◽  
Emma Crooke ◽  
Andrew Ross ◽  
Charlotte Stalvies ◽  
Trevor Bastow ◽  
...  
Keyword(s):  
Real Time ◽  
Aromatic Hydrocarbons ◽  
Water Samples ◽  
Sensor Array ◽  
Sea Water ◽  
Operating Conditions ◽  
Laboratory Evaluation ◽  
Sea Bed ◽  
Wide Range ◽  
Oil Components

Marine surveys for the detection of naturally seeping hydrocarbons require a wide range of complementary remote sensing and geochemical techniques in order to achieve reliable data interpretation and prediction. Compared with current geochemical techniques such as sniffers and sea bed head space gas analysis, oil-in-water hydrocarbon sensors can provide real-time chemical information. The use of these sensors, in combination with current methods, offers a potentially important aid in achieving an integrated approach. In this study, CSIRO Petroleum has constructed a hydrocarbon sensors array that is composed of three groups of sensors that target volatile mono-aromatic hydrocarbons, poly-aromatic hydrocarbons at low concentration and oil films, respectively. This configuration allows us to have a comprehensive map of hydrocarbon distribution in water samples in real time. The variety of response times, sensitivities and operating conditions of the sensing devices has been considered, in order to design a flow tank and develop suitable monitoring and control software for the array. Chemometric tools such as principle component analysis (PCA) are used for data analysis and prediction model creation.Results will be presented from the evaluation measurements carried out in three stages. These include: laboratory evaluation of the sensor array to the dissolved oil components of a series of crude oil samples in synthetic sea water; sensor responses to natural sea water samples; and, initial field studies during surveys in Australian sea waters. To our knowledge, there have been no previously recorded instances of using such a hydrocarbon sensor array for oil and gas exploration.

scholarly journals Extracellular Cl− modulates shrinkage-induced activation of Na+/H+exchanger in rat mesangial cells

2000 ◽  
Vol 278 (6) ◽  
pp. C1218-C1229 ◽  
Author(s):  
Yukio Miyata ◽  
Shigeaki Muto ◽  
Satoru Yanagiba ◽  
Yasushi Asano
Keyword(s):  
Benzoic Acid ◽  
Intracellular Ph ◽  
Recovery Rate ◽  
Mesangial Cells ◽  
Ph Sensitive ◽  
Extrusion Rate ◽  
Wide Range ◽  
Dependent Cell ◽  
Acid Extrusion ◽  
Dependent Processes

To examine the effect of hyperosmolality on Na+/H+ exchanger (NHE) activity in mesangial cells (MCs), we used a pH-sensitive dye, 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein-AM, to measure intracellular pH (pHi) in a single MC from rat glomeruli. All the experiments were performed in CO2/[Formula: see text]-free HEPES solutions. Exposure of MCs to hyperosmotic HEPES solutions (500 mosmol/kgH2O) treated with mannitol caused cell alkalinization. The hyperosmolality-induced cell alkalinization was inhibited by 100 μM ethylisopropylamiloride, a specific NHE inhibitor, and was dependent on extracellular Na+. The hyperosmolality shifted the Na+-dependent acid extrusion rate vs. pHi by 0.15–0.3 pH units in the alkaline direction. Removal of extracellular Cl− by replacement with gluconate completely abolished the rate of cell alkalinization induced by hyperosmolality and inhibited the Na+-dependent acid extrusion rate, whereas, under isosmotic conditions, it caused no effect on Na+-dependent pHi recovery rate or Na+-dependent acid extrusion rate. The Cl−-dependent cell alkalinization rate under hyperosmotic conditions was partially inhibited by pretreatment with 5-nitro-2-(3-phenylpropylamino)benzoic acid, DIDS, and colchicine. We conclude: 1) in MCs, hyperosmolality activates NHE to cause cell alkalinization, 2) the acid extrusion rate via NHE is greater under hyperosmotic conditions than under isosmotic conditions at a wide range of pHi, 3) the NHE activation under hyperosmotic conditions, but not under isosmotic conditions, requires extracellular Cl−, and 4) the Cl−-dependent NHE activation under hyperosmotic conditions partly occurs via Cl− channel and microtubule-dependent processes.

scholarly journals Quantification of Tetracycline Resistance Genes in Feedlot Lagoons by Real-Time PCR

2004 ◽  
Vol 70 (12) ◽  
pp. 7372-7377 ◽  
Author(s):  
Marilyn S. Smith ◽  
Richard K. Yang ◽  
Charles W. Knapp ◽  
Yafen Niu ◽  
Nicholas Peak ◽  
...  
Keyword(s):  
Real Time ◽  
Resistance Gene ◽  
Water Samples ◽  
Real Time Pcr ◽  
Tetracycline Resistance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lagoon Water ◽  
Tetracycline Resistance Genes ◽  
Wide Range ◽  
Pcr Method

ABSTRACT A new real-time PCR method is presented that detects and quantifies three tetracycline resistance (Tcr) genes [tet(O), tet(W), and tet(Q)] in mixed microbial communities resident in feedlot lagoon wastewater. Tcr gene real-time TaqMan primer-probe sets were developed and optimized to quantify the Tcr genes present in seven different cattle feedlot lagoons, to validate the method, and to assess whether resistance gene concentrations correlate with free-tetracycline levels in lagoon waters. The method proved to be sensitive across a wide range of gene concentrations and provided consistent and reproducible results from complex lagoon water samples. The log10 of the sum of the three resistance gene concentrations was correlated with free-tetracycline levels (r 2 = 0.50, P < 0.001; n = 18), with the geometric means of individual resistance concentrations ranging from 4- to 8.3-fold greater in lagoon samples with above-median tetracycline levels (>1.95 μg/liter by enzyme-linked immunosorbent assay techniques) than in below-median lagoon samples. Of the three Tcr genes tested, tet(W) and tet(Q) were more commonly found in lagoon water samples. Successful development of this real-time PCR assay will permit other studies quantifying Tcr gene numbers in environmental and other samples.

scholarly journals  Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR

2012 ◽  
Vol 57 (No. 5) ◽  
pp. 224-232 ◽  
Author(s):  
M. Adamska ◽  
A. Leonska-Duniec ◽  
M. Sawczuk ◽  
A. Maciejewska ◽  
B. Skotarczak
Keyword(s):  
Real Time ◽  
Water Samples ◽  
Real Time Pcr ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
High Concentration ◽  
Intestinal Protozoan ◽  
Wide Range ◽  
Stool Samples

Cryptosporidium parvum is a common intestinal protozoan parasite infecting humans and a wide range of animals, whose diagnostics present considerable difficulties. These arise from the exceptionally robust nature of the oocyst&rsquo;s walls, which necessitates more stringent treatments for disruption and recovery of DNA for analysis using molecular methods. In the case of water, which is the major source of Cryptosporidium oocysts, investigations concern the detection of the presence of the oocysts. Their concentration in water is very low, and moreover, many substances that may have significance as inhibitors of DNA amplification, are present in environmental water and stool. We have carried out trials in order to assess the effectiveness of recovery of C.&nbsp;parvum oocysts, from spiked environmental and distilled water samples, filtrated and concentrated with the use of special laboratory equipment. Inactivation of inhibitors was carried out with use of bovine serum albumin (BSA) in PCR mixes at ten different concentrations. DNA extraction was carried out from stool samples spiked with C.&nbsp;parvum oocysts, concentrated using two methods, and unconcentrated. Nested PCR and a TaqMan nested real time PCR assay, targeting the 18S rRNA gene, was used to detect C. parvum DNA in spiked water and additionally in spiked stool samples. The obtained results showed that losses of C. parvum oocysts occur during the filtration and concentration of spiked water samples. The addition of small amounts of BSA (5&ndash;20 ng/&micro;l) to PCR and TaqMan PCR mixes increases the sensitivity of both methods, but a high concentration of BSA (100 ng/&micro;l and above) has an inhibiting effect on the polymerase reaction. The extraction of DNA from C. parvum oocysts from spiked stool samples preceded by concentration with PBS, ether and Percoll resulted in a higher copy number of the 18S rRNA gene. &nbsp;

A dual stimuli responsive fluorescent probe carrier from a double hydrophilic block copolymer capped with β-cyclodextrin

2015 ◽  
Vol 6 (18) ◽  
pp. 3382-3386 ◽  
Author(s):  
Guang Yang ◽  
Zhen Yang ◽  
Chengguang Mu ◽  
Xiaodong Fan ◽  
Wei Tian ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Fluorescent Probe ◽  
Triblock Copolymer ◽  
Ph Sensitive ◽  
Stimuli Responsive ◽  
Self Assembling

A β-cyclodextrin terminated triblock copolymer with both hydrophilic temperature and pH sensitive segments was prepared and characterized in terms of self-assembling and encapsulation behaviors.

An off-on fluorescent probe for real-time sensing the fluctuations of intracellular pH values in biological processes

2019 ◽  
Vol 170 ◽  
pp. 107620 ◽  
Author(s):  
Jie Zhang ◽  
Jianguo Li ◽  
Bochao Chen ◽  
Jianfei Kan ◽  
Tiantian Jiang ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Biological Processes ◽  
Ph Values
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