Transfuse neonates at high risk for retinopathy of prematurity (ROP) with cord blood-derived red blood cells: A feasibility study to assess allogeneic cord blood (ACB) unit fractionation and neonatal blood transfusion request

Hematopoietic progenitor cell (HPC) transplantation is a treatment option for malignant and nonmalignant diseases. Umbilical cord blood (UCB) is an important HPC source, mainly for pediatric patients. It has been demonstrated that human leukocyte antigen (HLA) matching and cell dose are the most important features impacting clinical outcomes. However, UCB matching is performed using low resolution HLA typing and it has been demonstrated that the unnoticed mismatches negatively impact the transplant. Since we found differences in CD34+ viability after thawing of UCB units matched for two different patients (p = 0.05), we presumed a possible association between CD34+ cell viability and HLA. We performed a multivariate linear model (n = 67), comprising pre-cryopreservation variables and high resolution HLA genotypes separately. We found that pre-cryopreservation red blood cells (RBC), granulocytes, and viable CD34+ cell count significantly impacted CD34+ viability after thawing, along with HLA-B or -C (R2 = 0.95, p = 0.01; R2 = 0.56, p = 0.007, respectively). Although HLA-B*40:02 may have a negative impact on CD34+ cell viability, RBC depletion significantly improves it.

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Do erythropoietin and a blood transfusion guideline reduce the amount of packed red blood cells transfused in a major teaching hospital?

CHEST Journal ◽

10.1378/chest.126.4_meetingabstracts.871s-b ◽

2004 ◽

Vol 126 (4) ◽

pp. 871S

Author(s):

Nam T. Ly ◽

Brian Cuneo ◽

William L. Jackson ◽

Nagla Wahab ◽

Linda Gery ◽

...

Keyword(s):

Blood Transfusion ◽

Red Blood Cells ◽

Teaching Hospital ◽

Blood Cells ◽

Packed Red Blood Cells ◽

Major Teaching Hospital ◽

Major Teaching

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Retrolental Fibroplasia and Blood Transfusion in Very Low-Birth-Weight Infants

PEDIATRICS ◽

10.1542/peds.68.6.770 ◽

1981 ◽

Vol 68 (6) ◽

pp. 770-774 ◽

Cited By ~ 1

Author(s):

Linda M. Sacks ◽

David B. Schaffer ◽

Endla K. Anday ◽

George J. Peckham ◽

Maria Delivoria-Papadopoulos

Keyword(s):

Birth Weight ◽

Blood Transfusion ◽

Low Birth Weight ◽

Red Blood Cells ◽

Blood Cells ◽

Very Low Birth Weight ◽

Statistical Significance ◽

Low Birth Weight Infants ◽

Retrolental Fibroplasia ◽

Packed Red Blood Cells

The relative contribution of transfusions of adult blood to the development of retrolental fibroplasia (RLF) in very low-birth-weight infants was examined. Five years of experience with the expanded use of replacement and exchange transfusions in 90 infants with birth weight ≤1,250 gm was reviewed. Twenty percent of the infants developed cicatricial RLF. Exchange transfusion was not related to development of cicatricial RLF. The incidence of RLF in infants receiving ≥130 ml of packed red blood cells per kilogram of birth weight as replacement blood transfusion (RBT) was significantly higher (42.9%) than that in infants receiving 61 to 131 ml of packed red blood cells per kilogram (15.4%) and infants receiving ≤60 ml of packed red blood cells per kilogram (0%), P < .001. The need for RBT, however, was strongly correlated (r = .85, P < .001) with increasing duration of O2 therapy. When O2 therapy was controlled for, the association between RBT and RLF did not achieve statistical significance (P = .07). The association between RBT and RLF remained significant when adjusted for duration of therapy in fractional inspired oxygen (FIO2) >0.4. Further detailed studies of large numbers of susceptible infants are warranted to assess the magnitude of the contribution of transfusions of adult blood to development of RLF.

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Assay of nucleated red blood cells in the umbilical cord blood to assess the severity of outcome among the neonates born with meconium stained amniotic fluid

International Journal of Paediatrics and Geriatrics ◽

10.33545/26643685.2021.v4.i1b.129 ◽

2021 ◽

Vol 4 (1) ◽

pp. 62-65

Author(s):

Devendiran A ◽

Ramanathan R ◽

Manohar U

Keyword(s):

Amniotic Fluid ◽

Cord Blood ◽

Red Blood Cells ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Blood Cells ◽

Nucleated Red Blood Cells ◽

Meconium Stained Amniotic Fluid

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Prediction of perinatal asphyxia with nucleated red blood cells in cord blood of newborns

International Journal of Gynecology & Obstetrics ◽

10.1016/s0020-7292(03)00124-3 ◽

2003 ◽

Vol 81 (3) ◽

pp. 267-271 ◽

Cited By ~ 43

Author(s):

B. Ghosh ◽

S. Mittal ◽

S. Kumar ◽

V. Dadhwal

Keyword(s):

Cord Blood ◽

Red Blood Cells ◽

Blood Cells ◽

Perinatal Asphyxia ◽

Nucleated Red Blood Cells

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Potential use of autologous umbilical cord blood red blood cells for early transfusion needs of premature infants

Transfusion ◽

10.1111/j.1537-2995.2006.00841.x ◽

2006 ◽

Vol 46 (6) ◽

pp. 1049-1056 ◽

Cited By ~ 22

Author(s):

Marijke Jansen ◽

Anneke Brand ◽

Jeannette S. von Lindern ◽

Sicco Scherjon ◽

Frans J. Walther

Keyword(s):

Cord Blood ◽

Red Blood Cells ◽

Umbilical Cord ◽

Premature Infants ◽

Umbilical Cord Blood ◽

Blood Cells ◽

Potential Use

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The rates of ABO, Rh, and Kell antigens in children with cancer.

Journal of Clinical Oncology ◽

10.1200/jco.2017.35.15_suppl.e22007 ◽

2017 ◽

Vol 35 (15_suppl) ◽

pp. e22007-e22007

Author(s):

Andrey A. Maslov ◽

Nailya Guskova ◽

Ekaterina Guskova ◽

Kristina Avanesova ◽

Svetlana V. Belgova ◽

...

Keyword(s):

Blood Transfusion ◽

Red Blood Cells ◽

Blood Cells ◽

Subsequent Development ◽

European Population ◽

Blood Groups ◽

Abo Blood Groups ◽

Phenotypic Characteristics ◽

Children With Cancer ◽

K Antigen

e22007 Background: The purpose of the study was to analyze phenotypic characteristics of red blood cells by the AVBO, Rh and Kell systems in children with cancer. Methods: ABO and Rh blood groups were determined and erythrocyte antigens (D, С, с, Сw, Е, е, К, k) were typed (AutoVue Innova, USA) in blood samples of 114 children with solid tumors. Results: ABO blood groups distribution was as follows: A(II) > O(I) > B(III) > AB(IV) with A(II) prevalence. Rh(D)-positive phenotype was observed in 82 (71.9%) patients of 114: 47 (57.3%) boys and 35 (42.6%) girls. 32 (28.1%) patients of 114 were Rh(D)-negative: 15 (46.8%) boys and 17 (53.1%) girls. Only 8 (7%) children were Kell-positive, which was similar to the antigen prevalence in European population. 4 erythrocyte phenotypes were the most frequent in Rh(D)-positive patients: СсDееK− (34.1%), ССDееK− (22.0%), ccDEeK− (13.4%) and СсDЕеK− (11.0%). I.e., more than a half of children with oncopathologies (56.1%) had Kell-negative phenotypes, СсDееK− and ССDееK−. 86.4% of Rh(D)-positive patients had homozygous combinations of Rhesus antigens causing transfusion reactions - СС, сc, ЕЕ and ее. 18 (22.0%) of Rh(D)-positive patients were homozygous for the C antigen and 64 (78.0%), i.e. every third patient, had the c antigen. Children with the C antigen may be sensitized to the c antigen through blood transfusion with subsequent development of hemolytic complications. The K (Cellano) antigen was found in all children, and 93% of them had kk phenotype and 7% - Kk. The Сw (Willis) antigen was revealed only in 5 (6.0%) Rh(D)-positive patients with rare phenotypes - CwCceeK-, CwccEeK-, CwCcEEK-, CwCcEeK-. Matching a donor for patients with one of these phenotypes could pose a problem. Conclusions: Studying phenotypic characteristics of red blood cells is necessary for providing a successful blood transfusion, especially in children Kell-positive for the K antigen, in children homozygous for the C antigen with ССDееК- phenotype and in children with the Сw antigen and СwСсееК-, СwссЕеК-, СwСсЕЕК- and СwСсЕеК- phenotypes.

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