scholarly journals Sublethal effects of Isoclast™ Active (50% sulfoxaflor water dispersible granules) on larval and adult worker honey bees (Apis mellifera L.)

2021 ◽  
Vol 220 ◽  
pp. 112379
Author(s):  
Jiahuan Li ◽  
Liuwei Zhao ◽  
Suzhen Qi ◽  
Wenting Zhao ◽  
Xiaofeng Xue ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Sublethal Effects ◽  
Water Dispersible ◽  
Adult Worker

scholarly journals Review on Sublethal Effects of Environmental Contaminants in Honey Bees (Apis mellifera), Knowledge Gaps and Future Perspectives

2021 ◽  
Vol 18 (4) ◽  
pp. 1863 ◽  
Author(s):  
Agata Di Noi ◽  
Silvia Casini ◽  
Tommaso Campani ◽  
Giampiero Cai ◽  
Ilaria Caliani
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Sublethal Effects ◽  
Integrated Approach ◽  
Anthropogenic Contamination ◽  
Knowledge Gaps ◽  
Pollination Services ◽  
General Decline ◽  
Polycyclic Aromatic ◽  
The Impact

Honey bees and the pollination services they provide are fundamental for agriculture and biodiversity. Agrochemical products and other classes of contaminants, such as trace elements and polycyclic aromatic hydrocarbons, contribute to the general decline of bees’ populations. For this reason, effects, and particularly sublethal effects of contaminants need to be investigated. We conducted a review of the existing literature regarding the type of effects evaluated in Apis mellifera, collecting information about regions, methodological approaches, the type of contaminants, and honey bees’ life stages. Europe and North America are the regions in which A. mellifera biological responses were mostly studied and the most investigated compounds are insecticides. A. mellifera was studied more in the laboratory than in field conditions. Through the observation of the different responses examined, we found that there were several knowledge gaps that should be addressed, particularly within enzymatic and molecular responses, such as those regarding the immune system and genotoxicity. The importance of developing an integrated approach that combines responses at different levels, from molecular to organism and population, needs to be highlighted in order to evaluate the impact of anthropogenic contamination on this pollinator species.

scholarly journals Transcriptional Control of Honey Bee (Apis mellifera) Major Royal Jelly Proteins by 20-Hydroxyecdysone

Insects ◽  
2018 ◽  
Vol 9 (3) ◽  
pp. 122 ◽  
Author(s):  
Paul Winkler ◽  
Frank Sieg ◽  
Anja Buttstedt
Keyword(s):  
Gene Expression ◽  
Apis Mellifera ◽  
Juvenile Hormone ◽  
Honey Bee ◽  
Honey Bees ◽  
Transcriptional Control ◽  
Royal Jelly ◽  
Adult Worker ◽  
Gene Expression Dynamics

One of the first tasks of worker honey bees (Apis mellifera) during their lifetime is to feed the larval offspring. In brief, young workers (nurse bees) secrete a special food jelly that contains a large amount of unique major royal jelly proteins (MRJPs). The regulation of mrjp gene expression is not well understood, but the large upregulation in well-fed nurse bees suggests a tight repression until, or a massive induction upon, hatching of the adult worker bees. The lipoprotein vitellogenin, the synthesis of which is regulated by the two systemic hormones 20-hydroxyecdysone and juvenile hormone, is thought to be a precursor for the production of MRJPs. Thus, the regulation of mrjp expression by the said systemic hormones is likely. This study focusses on the role of 20-hydroxyecdysone by elucidating its effect on mrjp gene expression dynamics. Specifically, we tested whether 20-hydroxyecdysone displayed differential effects on various mrjps. We found that the expression of the mrjps (mrjp1–3) that were finally secreted in large amounts into the food jelly, in particular, were down regulated by 20-hydroxyecdysone treatment, with mrjp3 showing the highest repression value.

Identification of Mesophylic Bacterial Flora in Deceased Worker Adults of Apis mellifera caucasia (Pollmann, 1889)

Sociobiology ◽  
2021 ◽  
Vol 68 (1) ◽  
pp. 5905
Author(s):  
Elif Çil ◽  
Ömer Ertürk ◽  
Kamil Işik
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Phylogenetic Trees ◽  
Bacterial Flora ◽  
New Members ◽  
Important Species ◽  
Adult Worker ◽  
Study Results ◽  
Molecular Phylogenetic ◽  
Bacillus Genus

Apis mellifera, widely farmed around the world, is the most economically important species within the genus Apis. While the microbiota of live honey bees have been extensively examined, bacteria found in deceased honey bees (which might indicate infection or opportunistic pathogens) is in contrast poorly studied. Therefore, we decided to investigate the mesophilic bacterial flora of dead honey bees. So, in September 2013, dead adult worker honey bees were collected from 12 different cities, most of which were in the border provinces of Turkey. We identified bacterial isolates at the species level by using different morphological, biochemical, physical and molecular methods, in conjunction with molecular phylogenetic analysis. We constructed phylogenetic trees for isolated bacteria with the MEGA 6.0 program and neighbor-joining trees were reconstructed based on 16S rDNA gene sequences. The phylogenetic trees indicated that isolates DE003, DE007, DE011, DE001, DE019 and DE016, DE029 could be new members of the genera Erwinia, Acidovorax, Hydrogenophaga and Bacillus genus, respectively. In the bioassay study results, we observed that DE019 Hydrogenophaga sp. (64.7%) and DE004 Klebsiella grimontii (73.3%) had lethal effects on the honey bees. The other mortalities ranged from 10% to 25% (p>0.05), and according to a One-Way ANOVA analysis DE004 and DE019 significantly affect the A. mellifera caucasia in adult worker honey bees. This study is the first report of Hydrogenophaga as honey bee pathogen.

Comparison of juvenile hormone and ecdysteroid haemolymph titres in adult worker and queen honey bees (Apis mellifera)

1991 ◽  
Vol 37 (12) ◽  
pp. 929-935 ◽  
Author(s):  
Gene E. Robinson ◽  
Colette Strambi ◽  
Alain Strambi ◽  
Mark F. Feldlaufer
Keyword(s):  
Apis Mellifera ◽  
Juvenile Hormone ◽  
Honey Bees ◽  
Adult Worker

scholarly journals Field-Level Sublethal Effects of Approved Bee Hive Chemicals on Honey Bees (Apis mellifera L)

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e76536 ◽  
Author(s):  
Jennifer A. Berry ◽  
W. Michael Hood ◽  
Stéphane Pietravalle ◽  
Keith S. Delaplane
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Sublethal Effects ◽  
Field Level

scholarly journals Horizontal Honey-Bee Larvae Rearing Plates Can Increase the Deformation Rate of Newly Emerged Adult Honey Bees

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 603
Author(s):  
Juyeong Kim ◽  
Kyongmi Chon ◽  
Bo-Seon Kim ◽  
Jin-A Oh ◽  
Chang-Young Yoon ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Deformation Rate ◽  
Sublethal Effects ◽  
Horizontal Position ◽  
Control Groups ◽  
Honey Bee Larvae ◽  
Ideal Method

Rearing honey bee larvae in vitro is an ideal method to study honey bee larval diseases or the toxicity of pesticides on honey bee larvae under standardized conditions. However, recent studies reported that a horizontal position may cause the deformation of emerged bees. Accordingly, the purpose of this study was to evaluate the emergence and deformation rates of honey bee (Apis mellifera ligustica) larvae reared in horizontal and vertical positions. The study was conducted under the same laboratory conditions with three experimental groups, non-capped or capped horizontal plates and capped vertical plates. However, our results demonstrated that the exhibited adult deformation rates of the horizontal plates were significantly higher (27.8% and 26.1%) than those of the vertical plates (11.9%). In particular, the most common symptoms were deformed wings and an abnormal abdomen in the horizontal plates. Additionally, adults reared on horizontal plates were substantially smaller (10.88 and 10.82 mm) than those on vertical plates (11.55 mm). Considering these conclusions, we suggest that a vertical rearing method is more suitable when considering the deformation rates of the control groups to verify the sublethal effects of pesticides on honey bees.

scholarly journals Using video-tracking to assess sublethal effects of pesticides on honey bees (Apis mellifera L.)

2012 ◽  
Vol 31 (6) ◽  
pp. 1349-1354 ◽  
Author(s):  
Bethany S. Teeters ◽  
Reed M. Johnson ◽  
Marion D. Ellis ◽  
Blair D. Siegfried
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Sublethal Effects ◽  
Video Tracking

scholarly journals The influence of different diets on haemocytes of adult worker honey bees, Apis mellifera

Apidologie ◽  
2003 ◽  
Vol 34 (2) ◽  
pp. 97-102 ◽  
Author(s):  
Bozena Szyma? ◽  
Andrzej J?druszuk
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Adult Worker

scholarly journals A Modified Standardized Method to Extract and Store Insect Hemolymph with Use of a Glass Capillary

2020 ◽  
Vol 64 (1) ◽  
pp. 165-168 ◽  
Author(s):  
Paweł Migdał ◽  
Agnieszka Murawska ◽  
Adam Roman
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Physiological Saline ◽  
Extraction Methods ◽  
Glass Capillary ◽  
Pure Material ◽  
Adult Worker ◽  
Standardized Method ◽  
Basic Equipment

AbstractHemolymph is the “blood” circulating in the entire insect body. Analysis of this fluid gives information about the condition of the insect. The most precise analyses are conducted on insects’ hemolymph suspended in 0.6% physiological saline (NaCl). Most current hemolymph extraction methods are either difficult or do not provide pure material, the contamination of collected insect “blood” can change results. This study aimed to develop a technique for extract hemolymph, both easy and without risk of contaminating derived material. The presented method is a modification of available ways to extract, store and transport hemolymph with the use of a glass capillary. For the development of this technique, adult worker honey bees (Apis mellifera L.) were used. The method required such basic equipment as a glass capillary, sterile tweezer, Eppendorf tube and physiological saline. The collected hemolymph were frozen and hence sent in a glass capillary to another laboratory for analysis.

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