scholarly journals Biohydrogen production from cheese whey powder by Enterobacter asburiae: Effect of operating conditions on hydrogen yield and chemometric study of the fermentative metabolites

2020 ◽  
Vol 6 ◽  
pp. 1170-1180 ◽  
Author(s):  
Cecilia L. Alvarez-Guzmán ◽  
Sergio Cisneros-de la Cueva ◽  
Victor E. Balderas-Hernández ◽  
Adam Smoliński ◽  
Antonio De León-Rodríguez
2018 ◽  
Vol 7 (2) ◽  
pp. 101-109 ◽  
Author(s):  
Yumechris Amekan ◽  
Dyah Sekar A P Wangi ◽  
Muhammad Nur Cahyanto ◽  
Sarto Sarto ◽  
Jaka Widada

The natural microbial consortium from many sources widely used for hydrogen production. Type of substrate and operating conditions applied on the biodigesters of the natural consortium used as inoculum impact the variation of species and number of microbes that induce biogas formation, so this study examined the effect of different inoculum source and its combination of biohydrogen production performance. The hydrogen producing bacteria from fruit waste digester (FW), cow dung digester (CD), and tofu waste digester (TW) enriched under strictly anaerobic conditions at 37OC. Inoculums from 3 different digesters (FW, CD, and TW) and its combination (FW-CD, CD-TW, FW-TW, and FW-CD-TW) were used to test the hydrogen production from melon waste with volatile solids (VS) concentration of 9.65 g/L, 37°C and initial pH 7.05 ± 0.05. The results showed that individual and combined inoculum produced the gas comprising hydrogen and carbon dioxide without any detectable methane. The highest cumulative hydrogen production of 743 mL (yield 207.56 mL/gVS) and 1,132 mL (yield 231.02 mL/gVS) was shown by FW and FW-CD-TW, respectively. Butyric, acetate, formic and propionic were the primary soluble metabolites produced by all the cultures, and the result proves that higher production of propionic acid can decrease hydrogen yield. Clostridium perfringens and Clostridium baratii prominently seen in all single and combination inoculum. Experimental evidence suggests that the inoculum from different biodigesters able to adapt well to the environmental conditions and the new substrate after a combination process as a result of metabolic flexibility derived from the microbial diversity in the community to produce hydrogen. Therefore, inoculum combination could be used as a strategy to improve systems for on-farm energy recovery from animal and plant waste to processing of food and municipal waste.Article History: Received February 5th 2018; Received in revised form May 7th 2018; Accepted June 2nd 2018; Available onlineHow to Cite This Article: Amekan, Y., Wangi, D.S.A.P., Cahyanto, M.N., Sarto and Widada, J. (2018) Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste. Int. Journal of Renewable Energy Development, 7(2), 101-109.https://doi.org/10.14710/ijred.7.2.101-10


2021 ◽  
Vol 938 (1) ◽  
pp. 012017
Author(s):  
Yuriy V Litti ◽  
Elena A Zhuravleva ◽  
Andrey A Kovalev ◽  
Dmitriy A Kovalev ◽  
Inna V Katraeva ◽  
...  

Abstract The aim of this work was a comparative study of biohydrogen production from cheese whey and confectionary wastewater by a newly isolated thermophilic microbial strain Thermoanaerobacterium thermosaccharolyticum SP-H2. Experimental results showed that the fermentative hydrogen was successfully produced with the highest hydrogen yield of 3.9 mL H2/mL cheese whey or 80 mL H2/g chemical oxygen demand. The profile of soluble metabolite products showed that hydrogen generation by a new isolate was mainly acetate-type fermentation in the case of confectionary wastewater and mixed ethanol-acetate-lactate type fermentation in the case of cheese whey. The more optimal metabolic pathway of confectionary wastewater fermentation was confirmed by the better kinetic characteristics according to the Gompertz model.


2021 ◽  
Author(s):  
Antonio D N Ferraz ◽  
Laura Fuentes ◽  
Victoria de la Sovera ◽  
Patricia Bovio-Winkler ◽  
Felipe Eng ◽  
...  

Co-fermentation of cheese whey (CW) and thermal-alkaline pre-treated Yerba Mate (Ilex paraguariensis) waste (YMW) was performed aiming to produce biohydrogen and/or short- and medium-chain organic acids. Central Composite Designs (CCD) was chosen as the experimental design for evaluating the combinations of three independent variables namely YMW concentration, pH and inoculum concentration in hydrogen yield (H2Y; response variable). The increase of inoculum and YMW concentrations had positive effect in biohydrogen production and yield (H2Ymax of 1.35 mMH2.g-1 VS added) whereas the initial pH had no significant effect on it. Hydrogen was produced as a coproduct to butyrate mainly. Acetate from homoacetogenesis was accounted in all conditions evaluated. The CCD also indicated operating conditions to produce moderate-to-high concentrations of short and medium-chain organic acids such as butyrate (~135 mM), caproate (~45 mM) and lactate (~140 mM). 16S rRNA gene sequences analysis revealed five groups of microorganisms related to hydrogen, lactate and caproate production, ethanol-hydrogen co-production and hydrogen consumption.


Author(s):  
Kyungpyo Hong ◽  
Stephanie Nadya Sutanto ◽  
Jeong A. Lee ◽  
Jongsup Hong

Ni–Rh and Ni–Co nano-scale alloys exhibit high methane conversion, hydrogen yield, resistance to carbon formation, and long-term stability at low temperatures, allowing them to cope with the various operating conditions of direct methane-fueled PCFCs.


Author(s):  
Shuyang Zhang ◽  
Xiaoxin Wang ◽  
Peiwen Li

On-board hydrogen production via catalytic autothermal reforming is beneficial to vehicles using fuel cells because it eliminates the challenges of hydrogen storage. As the primary fuel for both civilian and military air flight application, Jet-A fuel (after desulfurization) was reformed for making hydrogen-rich fuels in this study using an in-house-made Rh/NiO/K-La-Ce-Al-OX ATR catalyst under various operating conditions. Based on the preliminary thermodynamic analysis of reaction equilibrium, important parameters such as ratios of H2O/C and O2/C were selected, in the range of 1.1–2.5 and 0.5–1.0, respectively. The optimal operating conditions were experimentally obtained at the reactor’s temperature of 696.2 °C, which gave H2O/C = 2.5 and O2/C = 0.5, and the obtained fuel conversion percentage, hydrogen yield (can be large than 1 from definition), and energy efficiency were 88.66%, 143.84%, and 64.74%, respectively. In addition, a discussion of the concentration variation of CO and CO2 at different H2O/C, as well as the analysis of fuel conversion profile, leads to the finding of effective approaches for suppression of coke formation.


2021 ◽  
Vol 11 (13) ◽  
pp. 5803
Author(s):  
Antonio Lara-Musule ◽  
Ervin Alvarez-Sanchez ◽  
Gloria Trejo-Aguilar ◽  
Laura Acosta-Dominguez ◽  
Hector Puebla ◽  
...  

Anaerobic treatment is a viable alternative for the treatment of agro-industrial waste. Anaerobic digestion reduces organic load and produces volatile fatty acids (VFA), which are precursors of value-added products such as methane-rich biogas, biohydrogen, and biopolymers. Nowadays, there are no low-cost diagnosis and monitoring systems that analyze the dynamic behavior of key variables in real time, representing a significant limitation for its practical implementation. In this work, the feasibility of using the multiscale analysis to diagnose and monitor the key variables in VFA production by anaerobic treatment of raw cheese whey is presented. First, experiments were carried out to evaluate the performance of the proposed methodology under different operating conditions. Then, experimental pH time series were analyzed using rescaled range (R/S) techniques. Time-series analysis shows that the anaerobic VFA production exhibits a multiscale behavior, identifying three characteristic regions (i.e., three values of Hurst exponent). In addition, the dynamic Hurst exponents show satisfactory correlations with the chemical oxygen demand (COD) consumption and VFA production. The multiscale analysis of pH time series is easy to implement and inexpensive. Hence, it could be used as a diagnosis and indirect monitoring system of key variables in the anaerobic treatment of raw cheese whey.


2006 ◽  
Vol 4 (4) ◽  
pp. 468-473 ◽  
Author(s):  
Alessandra Perna

The purpose of this work is to investigate, by a thermodynamic analysis, the effects of the process variables on the performance of an autothermal reforming (ATR)-based fuel processor, operating on ethanol as fuel, integrated into an overall proton exchange membrane (PEM) fuel cell system. This analysis has been carried out finding the better operating conditions to maximize hydrogen yield and to minimize CO carbon monoxide production. In order to evaluate the overall efficiency of the system, PEM fuel cell operations have been analyzed by an available parametric model.


Sign in / Sign up

Export Citation Format

Share Document