Potentialities of biotechnological recovery of hydrogen and short- and medium-chain organic acids from the co-fermentation of cheese whey and Yerba Mate (Ilex paraguariensis) waste

Co-fermentation of cheese whey (CW) and thermal-alkaline pre-treated Yerba Mate (Ilex paraguariensis) waste (YMW) was performed aiming to produce biohydrogen and/or short- and medium-chain organic acids. Central Composite Designs (CCD) was chosen as the experimental design for evaluating the combinations of three independent variables namely YMW concentration, pH and inoculum concentration in hydrogen yield (H2Y; response variable). The increase of inoculum and YMW concentrations had positive effect in biohydrogen production and yield (H2Ymax of 1.35 mMH2.g-1 VS added) whereas the initial pH had no significant effect on it. Hydrogen was produced as a coproduct to butyrate mainly. Acetate from homoacetogenesis was accounted in all conditions evaluated. The CCD also indicated operating conditions to produce moderate-to-high concentrations of short and medium-chain organic acids such as butyrate (~135 mM), caproate (~45 mM) and lactate (~140 mM). 16S rRNA gene sequences analysis revealed five groups of microorganisms related to hydrogen, lactate and caproate production, ethanol-hydrogen co-production and hydrogen consumption.

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Potentialities of biotechnological recovery of hydrogen and short- and medium-chain organic acids from the co-fermentation of cheese whey and Yerba Mate (Ilex paraguariensis) waste

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.113897 ◽

2021 ◽

Vol 171 ◽

pp. 113897

Author(s):

Antônio Djalma Nunes Ferraz ◽

Laura Fuentes ◽

Victoria de la Sovera ◽

Patricia Bovio-Winkler ◽

Felipe Eng ◽

...

Keyword(s):

Organic Acids ◽

Cheese Whey ◽

Ilex Paraguariensis ◽

Yerba Mate ◽

Medium Chain

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Biohydrogen production from cheese whey powder by Enterobacter asburiae: Effect of operating conditions on hydrogen yield and chemometric study of the fermentative metabolites

Energy Reports ◽

10.1016/j.egyr.2020.04.038 ◽

2020 ◽

Vol 6 ◽

pp. 1170-1180 ◽

Cited By ~ 1

Author(s):

Cecilia L. Alvarez-Guzmán ◽

Sergio Cisneros-de la Cueva ◽

Victor E. Balderas-Hernández ◽

Adam Smoliński ◽

Antonio De León-Rodríguez

Keyword(s):

Cheese Whey ◽

Operating Conditions ◽

Biohydrogen Production ◽

Hydrogen Yield ◽

Whey Powder ◽

Enterobacter Asburiae

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Optimization of Phenolics and Flavonoids Extraction Conditions and Antioxidant Activity of Roasted Yerba-Mate Leaves (Ilex paraguariensis A. St.-Hil., Aquifoliaceae) using Response Surface Methodology

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201420130019 ◽

2014 ◽

Vol 86 (2) ◽

pp. 923-934 ◽

Cited By ~ 24

Author(s):

DÉBORA C. BASSANI ◽

DOMINGOS S. NUNES ◽

DANIEL GRANATO

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Response Surface ◽

Desirability Function ◽

Operating Conditions ◽

Optimum Operating ◽

Ilex Paraguariensis ◽

Yerba Mate ◽

Experimental Conditions ◽

Highly Correlated

This study focused on maximizing the extraction of total phenolics and flavonoids as well as the antioxidant activity measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay from roasted yerba mate (Ilex paraguariensis) as a function of time (5, 7.5 and 10 min) and temperature of extraction (60, 75 and 90°C). The data were subjected to Response Surface Methodology and the results showed that polynomial equations were significant, did not present lack of fit, and presented adjusted determination coefficients above 98%, proving their suitability for prediction purposes. Using the desirability function, the optimum operating conditions to obtain a higher extraction of antioxidants was found to be 10 min of extraction at 90°C, and the tea prepared under these experimental conditions presented 427.74 mg of gallic acid equivalents per liter and 80.02% of inhibition of the DPPH radical. The flavonoid content was highly correlated (r = 0.9046, p < 0.001) to the antioxidant capacity.

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The capacity of wastewater treatment plants drives bacterial community structure and its assembly

Scientific Reports ◽

10.1038/s41598-019-50952-0 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Young Kyung Kim ◽

Keunje Yoo ◽

Min Sung Kim ◽

Il Han ◽

Minjoo Lee ◽

...

Keyword(s):

Wastewater Treatment ◽

Community Structure ◽

Bacterial Community ◽

Activated Sludge ◽

Community Assembly ◽

Bacterial Communities ◽

Wastewater Treatment Plants ◽

High Capacity ◽

Operating Conditions ◽

Rrna Gene

Abstract Bacterial communities in wastewater treatment plants (WWTPs) affect plant functionality through their role in the removal of pollutants from wastewater. Bacterial communities vary extensively based on plant operating conditions and influent characteristics. The capacity of WWTPs can also affect the bacterial community via variations in the organic or nutrient composition of the influent. Despite the importance considering capacity, the characteristics that control bacterial community assembly are largely unknown. In this study, we discovered that bacterial communities in WWTPs in Korea and Vietnam, which differ remarkably in capacity, exhibit unique structures and interactions that are governed mainly by the capacity of WWTPs. Bacterial communities were analysed using 16S rRNA gene sequencing and exhibited clear differences between the two regions, with these differences being most pronounced in activated sludge. We found that capacity contributed the most to bacterial interactions and community structure, whereas other factors had less impact. Co-occurrence network analysis showed that microorganisms from high-capacity WWTPs are more interrelated than those from low-capacity WWTPs, which corresponds to the tighter clustering of bacterial communities in Korea. These results will contribute to the understanding of bacterial community assembly in activated sludge processing.

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A Novel Microbial Zearalenone Transformation through Phosphorylation

Toxins ◽

10.3390/toxins13050294 ◽

2021 ◽

Vol 13 (5) ◽

pp. 294

Author(s):

Yan Zhu ◽

Pascal Drouin ◽

Dion Lepp ◽

Xiu-Zhen Li ◽

Honghui Zhu ◽

...

Keyword(s):

Microbial Transformation ◽

Acid Tolerance ◽

Transformation Product ◽

The United States ◽

Corn Silage ◽

Rrna Gene ◽

Bacillus Spp ◽

High Concentrations ◽

The 16S Rrna Gene ◽

Bacillus Strains

Zearalenone (ZEA) is a mycotoxin widely occurring in many agricultural commodities. In this study, a purified bacterial isolate, Bacillus sp. S62-W, obtained from one of 104 corn silage samples from various silos located in the United States, exhibited activity to transform the mycotoxin ZEA. A novel microbial transformation product, ZEA-14-phosphate, was detected, purified, and identified by HPLC, LC-MS, and NMR analyses. The isolate has been identified as belonging to the genus Bacillus according to phylogenetic analysis of the 16S rRNA gene and whole genome alignments. The isolate showed high efficacy in transforming ZEA to ZEA-14-phosphate (100% transformation within 24 h) and possessed advantages of acid tolerance (work at pH = 4.0), working under a broad range of temperatures (22–42 °C), and a capability of transforming ZEA at high concentrations (up to 200 µg/mL). In addition, 23 Bacillus strains of various species were tested for their ZEA phosphorylation activity. Thirteen of the Bacillus strains showed phosphorylation functionality at an efficacy of between 20.3% and 99.4% after 24 h incubation, suggesting the metabolism pathway is widely conserved in Bacillus spp. This study established a new transformation system for potential application of controlling ZEA although the metabolism and toxicity of ZEA-14-phosphate requires further investigation.

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Vegetative propagation, chemical composition and antioxidant activity of yerba mate genotypes

Plant Genetic Resources ◽

10.1017/s1479262121000150 ◽

2021 ◽

pp. 1-10

Author(s):

Leandro Marcolino Vieira ◽

Renata de Almeida Maggioni ◽

Jéssica de Cássia Tomasi ◽

Erik Nunes Gomes ◽

Ivar Wendling ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Vegetative Propagation ◽

Adventitious Rooting ◽

Ilex Paraguariensis ◽

Total Phenolic ◽

Yerba Mate ◽

Total Phenolic Compounds ◽

Leaf Retention ◽

Correlated Components

Abstract Ilex paraguariensis, commonly known as yerba mate, is a tree species native to South America. Its commercial value is due to the manufacturing of teas, with potential also in the pharmacological and cosmetic industries. Vegetative propagation of yerba mate is considered an innovation to the traditional production systems based on sexual propagation. The present study aimed to evaluate the rhizogenic potential and chemical attributes of mini-cuttings from 15 yerba mate genotypes, as well as to verify the correlation between phytochemical and rooting-related variables. Mini-cuttings were collected from a pre-existing mini-clonal hedge and the experimental design was completely randomized, with 15 treatments (genotypes), four replications and 10 mini-cuttings per plot. After 120 days, mini-cuttings were assessed regarding rooting, mortality, callogenesis and leaf retention percentages, percentage of mini-cuttings with both calluses and roots, number of roots and average root length. At the time of collection, subsamples from each plot were used for phytochemical analyses including total phenolic compounds, protein, caffeine and theobromine contents and antioxidant activity. Rooting percentages ranged from 5 to 72.5%, with significant variation among genotypes. Adventitious rooting and phytochemical profile of yerba mate mini-cuttings are genotype-dependent. Leaf retention is a relevant factor in the rooting of yerba mate mini-cuttings and the levels of total phenolic compounds, antioxidants and theobromine present in mini-cuttings are negatively correlated components to Ilex paraguariensis adventitious rooting.

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Ni-based bimetallic nano-catalysts anchored on BaZr0.4Ce0.4Y0.1Yb0.1O3−δ for internal steam reforming of methane in a low-temperature proton-conducting ceramic fuel cell

Journal of Materials Chemistry A ◽

10.1039/d0ta11359j ◽

2021 ◽

Author(s):

Kyungpyo Hong ◽

Stephanie Nadya Sutanto ◽

Jeong A. Lee ◽

Jongsup Hong

Keyword(s):

Steam Reforming ◽

Low Temperatures ◽

Operating Conditions ◽

Hydrogen Yield ◽

Carbon Formation ◽

Long Term Stability ◽

Ceramic Fuel Cell ◽

Ceramic Fuel ◽

Nano Catalysts

Ni–Rh and Ni–Co nano-scale alloys exhibit high methane conversion, hydrogen yield, resistance to carbon formation, and long-term stability at low temperatures, allowing them to cope with the various operating conditions of direct methane-fueled PCFCs.

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PERSISTÊNCIA NO SOLO DE INÓCULO DE Beauveria bassiana EMPREGADO PARA O CONTROLE DE Hedypathes betulinus EM PLANTIO DE Ilex paraguariensis

FLORESTA ◽

10.5380/rf.v41i1.21194 ◽

2011 ◽

Vol 41 (1) ◽

Author(s):

Larissa Rolim Borges ◽

Sônia Maria Noemberg Lazzari ◽

Ida Chapaval Pimentel ◽

Lívia Cardoso Bueno de Camargo ◽

Lílian Mattos Kalinoski

Keyword(s):

Beauveria Bassiana ◽

Entomopathogenic Fungus ◽

Abiotic Factors ◽

Agricultural Practices ◽

Control Measures ◽

Ilex Paraguariensis ◽

Natural Occurrence ◽

Yerba Mate ◽

Santa Catarina ◽

Wood Borer

O controle da broca-da-erva-mate, Hedypathes betulinus, limita-se ao emprego de práticas silviculturais e catação manual dos insetos adultos, não sendo permitido o uso de inseticidas químicos na cultura. Esta pesquisa teve por objetivos verificar a presença natural de Beauveria bassiana no solo de plantio em monocultura de erva-mate, determinar a persistência de B. bassiana no solo após duas e três aplicações do fungo e averiguar a influência de fatores abióticos na estabilidade do fungo no solo. O estudo foi conduzido em um erval no município de Campo Alegre, Santa Catarina, Brasil, onde foram coletadas amostras de solo em duas áreas, para avaliação da presença natural de inóculos do fungo B. bassiana, procedendo-se à contagem, isolamento e identificação.Não foi detectada a ocorrência natural do fungo no solo em nenhuma das duas áreas. Após essa confirmação, foram realizadas pulverizações com B. bassiana em ambas as áreas, sendo que em uma ocorreram duas e na outra três aplicações. Após a última pulverização, realizada em fevereiro/2006, verificou-se que os inóculos do fungo persistiram por 90 dias na área com duas aplicações e 120 dias na área com três aplicações. A temperatura afetou a persistência do fungo no solo em ambas as áreas, mas a umidade relativa não mostrou influência sobre os inóculos em campo.Palavras-chave: Broca-da-erva-mate; controle biológico; epizootia; fungo entomopatogênico. AbstractPersistence in soil of inoculum of Beauveria bassiana used to control Hedypathes betulinus in yerba maté plantation, Ilex paraguariensis. Control measures of Hedypathes betulinus are restricted to agricultural practices and hand picking of adult insects. No chemical control is allowed in yerba maté cultivation areas. The objectives of this research were to verify the natural occurrence of the entomopathogenic fungus Beauveria bassiana in the soil of yerba maté in monoculture; to determine the persistence of inoculums in the soil after applications of the fungus; and to evaluate the influence of abiotic factors on fungus stability in the soil. The soil samples were taken from two experimental areas in a yerba maté plantation in the county of Campo Alegre, Santa Catarina, Brazil. The samples were analyzed in order to count, isolate and identify inoculums of B. bassiana and other fungi. After the confirmation that B. bassiana was not present in the soil, a suspension of the fungus was applied, twice in one of the areas and three times in the other. After the last application in February/2006, the inoculum persisted for 90 and 120 days, in the area with two and three applications, respectively. The temperature affected the persistence of B. bassiana in soil; however, the relative humidity did not.Keywords: Biological control; epizooty; entomopathogenic fungus; wood borer.

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