Second-Derivative Synchronous Fluorescence Spectroscopy for the Simultaneous Determination of Cinnarizine and Nicergoline in Pharmaceutical Preparations

Abstract A rapid, simple, and highly sensitive second-derivative synchronous fluorometric method has been developed for the simultaneous analysis of binary mixtures of cinnarizine (CN) and nicergoline (NIC). The method is based upon measurement of the native fluorescence of these drugs at constant wavelength difference () = 80 nm in aqueous methanol (50, v/v). The different experimental parameters affecting the native fluorescence of the studied drugs were carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the range of 0.0251.5 and 0.255.5 g/mL for CN and NIC, respectively, with lower detection limits of 0.58 and 0.82 ng/mL and quantitation limits of 1.93 and 2.73 ng/mL for CN and NIC, respectively. The proposed method was successfully applied for the determination of the studied compounds in synthetic mixtures and in commercial tablets. The results obtained were in good agreement with those obtained with reference methods. The high sensitivity attained by the proposed method allowed the determination of CN in real and spiked human plasma. The mean recovery in the case of spiked human plasma [number of trials (n) = 3] was 102.82 2.17, while that in real human plasma (n = 3) was 105.25 2.05.

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Second Derivative Synchronous Fluorescence Spectroscopy for the Simultaneous Determination of Metoclopramide and Pyridoxine in Syrup and Human Plasma

Journal of AOAC International ◽

10.1093/jaoac/91.3.542 ◽

2008 ◽

Vol 91 (3) ◽

pp. 542-550 ◽

Cited By ~ 16

Author(s):

Nahed El-Enany

Keyword(s):

Simultaneous Determination ◽

Human Plasma ◽

High Sensitivity ◽

Second Derivative ◽

Native Fluorescence ◽

Highly Sensitive ◽

Experimental Parameters ◽

The Mean ◽

Good Agreement

Abstract A rapid, simple, and highly sensitive second derivative synchronous fluorometric method has been developed for the simultaneous determination of metoclopramide (MT) and pyridoxine (PY) in a binary mixture. The method is based on measurement of the native fluorescence of these drugs at = 80 nm in methanol. The different experimental parameters affecting the native fluorescence of the drugs were carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the ranges of 0.020.4 and 0.12 g/mL for MT and PY, respectively. The limits of detection were 0.003 and 0.007 g/mL and the limits of quantification were 0.008 and 0.02 g/mL for MT and PY, respectively. The proposed method was successfully applied to the determination of MT and PY in synthetic mixtures and in commercial syrup. The results were in good agreement with those obtained with a reported method. The high sensitivity attained by the proposed method allowed the determination of MT in spiked and real human plasma samples. The mean percent recoveries of MT from spiked and real human plasma (n = 3) were 93.72 3.15 and 89.72 2.19, respectively.

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Highly sensitive spectrofluorimetric determination of lomefloxacin in spiked human plasma, urine and pharmaceutical preparations

European Journal of Medicinal Chemistry ◽

10.1016/j.ejmech.2009.02.019 ◽

2009 ◽

Vol 44 (9) ◽

pp. 3402-3405 ◽

Cited By ~ 12

Author(s):

Sevgi Tatar Ulu

Keyword(s):

Human Plasma ◽

Pharmaceutical Preparations ◽

Spiked Human Plasma ◽

Highly Sensitive ◽

Spectrofluorimetric Determination

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Spectrofluorometric Determination of Verapamil Hydrochloride in Pharmaceutical Preparations and Human Plasma Using Organized Media: Application to Stability Studies

Journal of AOAC International ◽

10.1093/jaoac/89.6.1565 ◽

2006 ◽

Vol 89 (6) ◽

pp. 1565-1572 ◽

Cited By ~ 7

Author(s):

Mohamed Walash ◽

Fathalla Belal ◽

Nahed El-Enany ◽

Amina Abdelsalam

Keyword(s):

Human Plasma ◽

Biological Fluids ◽

Sodium Dodecyl ◽

Pharmaceutical Preparations ◽

Pharmaceutical Formulations ◽

Official Method ◽

Stability Studies ◽

Verapamil Hydrochloride ◽

Spiked Human Plasma

Abstract A highly sensitive spectrofluorometric method was developed for the determination of verapamil hydrochloride (VP HCl) in pharmaceutical formulations and biological fluids. The proposed method is based on investigation of the fluorescence spectral behavior of VP HCl in micellar systems, such as sodium dodecyl sulfate (SDS) and β-cyclodextrin (β-CD). In aqueous solutions of borate buffer of pH 9 and 8.5, VP HCl was well incorporated into SDS and β-CD, respectively, with enhancement of its native fluorescence. The fluorescence was measured at 318 nm after excitation at 231 nm. The fluorescence intensity enhancements were 183 and 107% in SDS and in β-CD, respectively. The fluorescence-concentration plots were rectilinear over the range of 0.020.2 and 0.020.25 μg/mL, with lower detection limits of 5.58 × 103 and 3.62 × 103 μg/mL in SDS and β-CD, respectively. The method was successfully applied to the analysis of commercial tablets and the results were in good agreement with those obtained with the official method. The method was further applied to the determination of VP HCl in real and spiked human plasma. The mean % recoveries in the case of spiked human plasma (n 4) was 92.59 3.11 and 88.35 2.55 using SDS and β-CD, respectively, while that in real human plasma (n 3) was 90.17 6.93 and 89.17 6.50 using SDS and β-CD, respectively. The application of the method was extended to the stability studies of VP HCl after exposureto ultraviolet radiation and upon oxidation with hydrogen peroxide.

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High performance liquid chromatographic determination of some co-administered anticancer drugs in pharmaceutical preparations and in spiked human plasma

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/s0731-7085(03)00655-1 ◽

2004 ◽

Vol 34 (5) ◽

pp. 1099-1107 ◽

Cited By ~ 32

Author(s):

Ossama T Fahmy ◽

Mohamed A Korany ◽

Hadir M Maher

Keyword(s):

Human Plasma ◽

Anticancer Drugs ◽

High Performance ◽

Pharmaceutical Preparations ◽

Chromatographic Determination ◽

High Performance Liquid Chromatographic ◽

Spiked Human Plasma ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

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Facile complexation reactions for the selective spectrofluorimetric determination of albendazole in oral dosage forms and spiked human plasma

RSC Advances ◽

10.1039/c7ra12360d ◽

2018 ◽

Vol 8 (10) ◽

pp. 5373-5381 ◽

Cited By ~ 3

Author(s):

Ahmed A. Hamad ◽

Ramadan Ali ◽

Hassan Refat H. Ali ◽

Dalia M. Nagy ◽

Sayed M. Derayea

Keyword(s):

Human Plasma ◽

Dosage Forms ◽

Oral Dosage ◽

Native Fluorescence ◽

Spiked Human Plasma ◽

Erythrosine B ◽

Spectrofluorimetric Determination ◽

Complexation Reactions ◽

Oral Dosage Forms

Complexation of albendazole with erythrosine B quench the native fluorescence of the dye while complexation of the drug with lanthanum (iii) ions enhance the fluorescence of the drug.

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A fast spectrofluorimetric method for determination of carbinoxamine maleate in the nano-molar range. Application to pharmaceutical preparations, biological fluids and stability studies

Analytical Methods ◽

10.1039/c8ay01202d ◽

2018 ◽

Vol 10 (31) ◽

pp. 3851-3858 ◽

Cited By ~ 1

Author(s):

Fatma Ahmed Aly ◽

Nahed EL-Enany ◽

Heba Elmansi ◽

Amany Nabil

Keyword(s):

Human Plasma ◽

Biological Fluids ◽

Pharmaceutical Preparations ◽

Pure Form ◽

Stability Studies ◽

Spiked Human Plasma ◽

Spectrofluorimetric Method

Carbinoxamine maleate (CBX), which is a common ingredient of cold and cough treatment preparations, is determined by a sensitive, simple and convenient spectrofluorimetric method in its pure form, pharmaceutical preparations and spiked human plasma.

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Development and validation of a highly sensitive second derivative synchronous fluorescence spectroscopic method for the simultaneous determination of elbasvir and grazoprevir in pharmaceutical preparation and human plasma

New Journal of Chemistry ◽

10.1039/d0nj03636f ◽

2020 ◽

Vol 44 (43) ◽

pp. 18679-18685

Author(s):

Khalid A. M. Attia ◽

Ahmed El-Olemy ◽

Sherif Ramzy ◽

Ahmed H. Abdelazim ◽

Mohamed A. Hasan ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Simultaneous Determination ◽

Human Plasma ◽

Pharmaceutical Preparation ◽

Spectroscopic Method ◽

Second Derivative ◽

Highly Sensitive ◽

Development And Validation

Elbasvir and grazoprevir combination has been newly approved for the treatment of patients infected with hepatitis C virus.

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Measurements of spectral reflectivity of manganese oxides

Mineralogical Magazine and Journal of the Mineralogical Society ◽

10.1180/minmag.1965.035.269.23 ◽

1965 ◽

Vol 35 (269) ◽

pp. 200-213 ◽

Cited By ~ 10

Author(s):

Ian Nichol ◽

Roy Phillips

Keyword(s):

Manganese Oxide ◽

White Light ◽

Manganese Oxides ◽

High Sensitivity ◽

Direct Reading ◽

Spectral Reflectivity ◽

Highly Sensitive ◽

Sensitive Method ◽

Good Agreement

SummaryAn accurate and highly sensitive method for the measurement of reflectivities incorporating a photomultiplier and a direct-reading galvanometer is described. The high sensitivity of the technique permits the determination of spectral reflectivities as opposed to ‘white’ light reflectivities, the latter values being subject to variation according to the type of apparatus used in their determination. The absolute spectral reflectivities of pyrite determined by the method are in good agreement with those quoted by some previous workers. Spectral reflectivities in air and oil and refractive and absorption indices are given for wavelengths from 430–650 mµ for manganese oxide minerals.

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Kinetic Spectrophotometric Determination of Ethamsylate in Dosage Forms

Journal of AOAC International ◽

10.1093/jaoac/90.3.679 ◽

2007 ◽

Vol 90 (3) ◽

pp. 679-685 ◽

Cited By ~ 6

Author(s):

Nahed El-Enany ◽

Fathalla Belal ◽

Mohamed Rizk

Keyword(s):

Room Temperature ◽

Kinetic Method ◽

Pharmaceutical Preparations ◽

Reference Method ◽

Quantitation Limit ◽

Experimental Parameters ◽

Kinetic Spectrophotometric ◽

Good Agreement

Abstract A simple and sensitive kinetic method has been developed for the determination of ethamsylate (ESL) in its pharmaceutical preparations. The method is based upon oxidation of ESL with 3-methyl-2-benzothiazolinone hydrazone hydrochloride in presence of cerium(IV) ammonium sulfate at room temperature for 20 min. The absorbance of the reaction product is measured at 514 nm. The absorbance-concentration plot was rectilinear over the range of 430 g/mL (r = 0.9999). The lower detection limit was 0.267 g/mL (9.110 106 M) and the lower quantitation limit was 0.808 g/mL. The different experimental parameters affecting the development and stability of the reaction product were studied and optimized. The proposed method was applied to the determination of ESL in formulations, and the results obtained were in good agreement with those obtained using a reference method. The proposed method was also used for the in vitro detection of ESL in spiked human plasma at its therapeutic concentration level.

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