Metformin modulates PI3K and GLUT4 expression and Akt/PKB phosphorylation in human endometrial stromal cells after stimulation with androgen and insulin

ABSTRACT 65 endometrial biopsies from castrated women who had received either natural or artificial sex hormone therapy were studied microscopically. Attention was paid to various histologic criteria, especially to the number of endometrial granulocytes (»K« cells, KZ). The following was obtained: The »K« cells are completely absent when no hormone substitution therapy is given. They were also lacking when the castrated patients were treated only with oestrogens, even if the dose given was ten-times that found in women during the reproductive ages. In contrast, the »K« cells developed from the endometrial stromal cells only under influence of progesterone, usually appearing first 8–10 days after the administration of the gestagen. The »K« cells were demonstrable in the number corresponding to a normal secretory phase only then, when the oestrogen-progesterone dosage ratio had induced a fully-developed secretory change, as measured by the usual histologic criteria. With an overdosage of oestrogen the »K« cells were either absent or were very sparse. Contrarily, an overdosage of progesterone had no influence on their number. The development of endometrial glands does not always entirely parallel that of the stroma in castrated patients following hormone therapy. A more exact indicator for the proper dose for the production of a secretory phase by hormone therapy seems to be the number of »K« cells in the endometrial stroma.

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Inhibition of TMEM16A impedes embryo implantation and decidualization in mice

Reproduction ◽

10.1530/rep-18-0197 ◽

2018 ◽

Cited By ~ 2

Author(s):

Qianrong Qi ◽

Yifan Yang ◽

Kailin Wu ◽

Qingzhen Xie

Keyword(s):

Progesterone Receptor ◽

Stromal Cells ◽

Embryo Implantation ◽

Mouse Uterus ◽

Endometrial Stromal Cells ◽

Uterine Endometrium ◽

Molecule Inhibitor ◽

Pathway Inhibition ◽

And Function ◽

Reproductive Processes

Recent studies revealed that TMEM16A is involved in several reproductive processes, including ovarian estrogen secretion and ovulation, sperm motility and acrosome reaction, fertilization, and myometrium contraction. However, little is known about the expression and function of TMEM16A in embryo implantation and decidualization. In this study, we focused on the expression and regulation of TMEM16A in mouse uterus during early pregnancy. We found that TMEM16A is up-regulated in uterine endometrium in response to embryo implantation and decidualization. Progesterone treatment could induce TMEM16A expression in endometrial stromal cells through progesterone receptor/c-Myc pathway, which is blocked by progesterone receptor antagonist or the inhibitor of c-Myc signaling pathway. Inhibition of TMEM16A by small molecule inhibitor (T16Ainh-A01) resulted in impaired embryo implantation and decidualization in mice. Treatment with either specific siRNA of Tmem16a or T16Ainh-A01 inhibited the decidualization and proliferation of mouse endometrial stromal cells. In conclusion, our results revealed that TMEM16A is involved in embryo implantation and decidualization in mice, compromised function of TMEM16A may lead to impaired embryo implantation and decidualization.

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Effect of flax seed (Linum usitatissimum) extract on modulation of prostaglandin E2 production by buffalo endometrial stromal cells cultured in vitro

Reproduction Abstracts ◽

10.1530/repabs.1.p307 ◽

2014 ◽

Author(s):

Singh Sanjay Kumar ◽

Chethan G Sharma ◽

Jessiehun Nongsiej ◽

R P Singh ◽

Agarwal Sudhir Kumar

Keyword(s):

Prostaglandin E2 ◽

Stromal Cells ◽

Linum Usitatissimum ◽

Flax Seed ◽

Endometrial Stromal Cells ◽

Cells Cultured

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Glucocorticoids trigger macrophage migration inhibitory factor (MIF) secretion by decidualized human endometrial stromal cells in vitro: the modulatory effect of Bisphenol A

Reproduction Abstracts ◽

10.1530/repabs.1.p276 ◽

2014 ◽

Author(s):

Chiara Mannelli ◽

Anna Szostek ◽

Sara Ciaffarafa ◽

Claudiopietro Carotenuto ◽

Francesca Ietta ◽

...

Keyword(s):

Bisphenol A ◽

Migration Inhibitory Factor ◽

Stromal Cells ◽

Macrophage Migration Inhibitory Factor ◽

Inhibitory Factor ◽

Macrophage Migration ◽

Endometrial Stromal Cells

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The expression of Bcl-2 in adenomyosis and its effect on proliferation, migration, and apoptosis of endometrial stromal cells

Pathology - Research and Practice ◽

10.1016/j.prp.2019.152477 ◽

2019 ◽

Vol 215 (8) ◽

pp. 152477 ◽

Cited By ~ 6

Author(s):

Junyan Li ◽

Ma Yanyan ◽

Lin Mu ◽

Xuejun Chen ◽

Wei Zheng

Keyword(s):

Stromal Cells ◽

Endometrial Stromal Cells

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Time Series Analysis of Transmesothelial Invasion by Endometrial Stromal and Epithelial Cells Using Three-dimensional Confocal Microscopy

Microscopy and Microanalysis ◽

10.1017/s143192760002897x ◽

2001 ◽

Vol 7 (S2) ◽

pp. 580-581

Author(s):

CA Witz ◽

S Cho ◽

VE Centonze ◽

IA Montoya-Rodriguez ◽

RS Schenken

Keyword(s):

Epithelial Cells ◽

Stromal Cells ◽

Time Course ◽

Three Dimensional ◽

Collagen Iv ◽

Mesothelial Cells ◽

Endometrial Stromal Cells ◽

Human Collagen ◽

Endometrial Epithelial Cells

Using human peritoneal explants, we have previously demonstrated that endometrial stromal cells (ESCs) and endometrial epithelial cells (EECs) attach to intact mesothelium. Attachment occurs within one hour and mesothelial invasion occurs within 18 hours (Figure 1). We have also demonstrated that, in vivo, the mesothelium overlies a continuous layer of collagen IV (Col IV).More recently we have used CLSM, to study the mechanism and time course of ESC and EEC attachment and invasion through mesothelial monolayers. in these studies, CellTracker® dyes were used to label cells. Mesothelial cells were labeled with chloromethylbenzoylaminotetramethylrhodamine (CellTracker Orange). Mesothelial cells were then plated on human collagen IV coated, laser etched coverslips. Mesothelial cells were cultured to subconfluence. ESCs and EECs, labeled with chloromethylfluorscein diacetate (CellTracker Green) were plated on the mesothelial monolayers. Cultures were examined at 1, 6, 12 and 24 hours with simultaneous differential interference contrast and CLSM.

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