The impact of high anxiety level on the oxidative status of mouse peripheral blood lymphocytes, granulocytes and monocytes

2008 ◽  
Vol 589 (1-3) ◽  
pp. 173-175 ◽  
Author(s):  
Hassan Rammal ◽  
Jaouad Bouayed ◽  
Chafique Younos ◽  
Rachid Soulimani
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Oxidative Status ◽  
Anxiety Level ◽  
High Anxiety ◽  
Blood Lymphocytes ◽  
The Impact

scholarly journals Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 658 ◽  
Author(s):  
Gabriela Gajek ◽  
Beata Marciniak ◽  
Jarosław Lewkowski ◽  
Renata Kontek
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Caffeic Acid Phenethyl Ester ◽  
Peripheral Blood Lymphocytes ◽  
Neoplastic Cells ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Apoptotic Activity ◽  
The Impact

The incidence of gastrointestinal cancers is increasing every year. Irinotecan (CPT-11), a drug used in the treatment of colorectal cancer and gastric cancer, is metabolized by carboxylesterases to an active metabolite, SN-38, which is more cytotoxic. CAPE (caffeic acid phenethyl ester) is an active component of propolis, which has a high antibacterial, antiviral, and antineoplastic potential. This study analyses the impact of CAPE on the cytotoxic (MTT assay), genotoxic (comet assay) and proapoptotic (caspase-3/7 activity) potential of irinotecan and its metabolite SN-38 in cultures of gastrointestinal neoplastic cells (HCT116, HT29, AGS). Cytotoxicity and genotoxicity activities of these compounds were carried out in comparison with human peripheral blood lymphocytes (PBLs) in vitro. The antioxidant potential of CAPE was investigated in relation H2O2-induced oxidative stress in the both neoplastic cells and PBLs. CAPE expressed cytotoxic, genotoxic, and pro-apoptotic activity against AGS, HCT116, and HT29 tumor cells. CAPE, in the presence of different concentrations of irinotecan or SN38, decreased the cytotoxicity, genotoxicity, and pro-apoptotic activity in these cell lines, but it has no such action on normal human peripheral blood lymphocytes.

Splenectomy selectively affects the distribution and mobility of the recirculating lymphocyte pool

Blood ◽  
2000 ◽  
Vol 96 (3) ◽  
pp. 1180-1183 ◽  
Author(s):  
Tim J. Seabrook ◽  
Wayne R. Hein ◽  
Lisbeth Dudler ◽  
Alan J. Young
Keyword(s):  
Lymph Nodes ◽  
Peripheral Blood ◽  
Blood Lymphocyte ◽  
Lymphocyte Subsets ◽  
Immune Surveillance ◽  
Peripheral Blood Lymphocytes ◽  
Immune Competence ◽  
Blood Lymphocytes ◽  
The Impact ◽  
Residency Time

The spleen plays a major role in immune surveillance, but the impact that splenectomy exerts on the immune competence of an individual is not fully resolved. Here we show that neonatal splenectomy in sheep does not abrogate the development of a large, nonrecirculating pool of lymphocytes and that it has no effect on the acquisition of a normal blood lymphocyte profile. Splenectomy did, however, result in a significant decrease in blood residency time of recirculating lymphocytes and in an enhanced accumulation of recirculating lymphocytes in lymph nodes. Furthermore, nonrecirculating peripheral blood lymphocytes were less likely to migrate to the lung, possibly because of saturation of the marginal pool by recirculating lymphocytes. Although splenectomy has little effect on the development or distribution of lymphocyte subsets in blood and lymph, it has marked effects on the rate of recirculation of lymphocytes, which may have significant implications for peripheral immune surveillance in patients who undergo splenectomy.

scholarly journals The impact of astaxanthin on radiation-induced chromosome aberrations in human peripheral blood lymphocytes in vitro

2016 ◽  
Vol 14 (1) ◽  
pp. 52-57
Author(s):  
M. A. Pilinska ◽  
D. A. Kurinnyi ◽  
S. R. Rushkovsky ◽  
O. B. Dybska
Keyword(s):  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Metaphase Chromosomes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Radiation Induced ◽  
The Impact

Aim. Research objective is to establish the possibility of modifying the astaxanthin (a carotenoid from a xanthophyll group) radiation-induced cytogenetic effects in human peripheral blood lymphocytes (PBLs) in vitro. Methods. The cultivation of PBLs from four conventionally healthy volunteers, the preparation and analysis of uniformly stained slides of metaphase chromosomes. Astaxanthin in final concentrations of 2, 10 and 20 µg/ml was added into the culture of PBL prior to the incubation before irradiation with γ-quanta in a dose of 1 Gy. Results. Astaxanthin did not affect the level and spectrum of chromosome damage in non-irradiated PBLs both in individual persons, and along the group on average (P>0.05), indicating a lack of mutagenic activity. The effect of astaxanthin at a concentration of 20 μg/ml on irradiated PBLs resulted in a significant reduction of radioinduced cytogenetic effect in all donors. Medium-group level of chromosome aberrations decreased almost 3 times and was characterized by statistically significant (P<0.001) decrease in frequency of chromosomal type aberrations due to the classical unstable cytogenetic markers of radiation effect, dicentrics and ring chromosomes. Conclusions. Astaxanthin at a concentration of 20 µg/ml was found to reduce the mutagenic effect of ionizing radiation, thus suggesting its powerful radioprotective potential. Keywords: astaxanthin, culture of human peripheral blood lymphocytes, radiation mutagenesis, chromosome aberrations, radioprotective effect.

scholarly journals Властивості Na+ , K+ -активованої, Mg2+ -залежної АТФ-гідролази лімфоцитів крові у хворих на реактивний артрит

2013 ◽  
Vol 4 (2) ◽  
pp. 57-62
Author(s):  
O.V. Melnyk ◽  
O.P. Kornijchuk ◽  
O.I. Pershyn ◽  
Z.D. Vorobets
Keyword(s):  
Atpase Activity ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Immunocompetent Cells ◽  
Affinity Constant ◽  
Kinetic Properties ◽  
Blood Lymphocytes ◽  
Healthy Donors ◽  
Immunological Mechanisms ◽  
The Impact

A significant role in the development and course of reactive arthritis (ReA) is played by T-lymphocytes as their development and systemic manifestations are based on immunological mechanisms. Additionally, the pathogenesis of many diseases is linked to changes in the structure and function of ion-transporting systems. Therefore, the aim of the study was to find out the kinetic properties of ATP-hydrolysis reaction involving Na+,K+-ATPase of peripheral blood lymphocytes of healthy individuals and patients with ReA. We used the current methodological approaches to the study of ATPase activity in saponin permeabilized cells. We conducted an analysis of the kinetic properties of ouabainsensitive Na+,K+-ATPase activity of saponin-perforated peripheral blood lymphocytes of healthy donors and patients with rheumatoid arthritis (RеA). We found out that in peripheral blood lymphocytes of patients with RеA primary active transport of Na+, K+ ions is slower and less intensive, though characterised by the same capacity, as in healthy donors. The affinity constant for ATP in peripheral blood lymphocytes in patients with RеA is greater by 2.9 times than its value in comparison with healthy donors. We established that in conditions of rheumatic pathology in immunocompetent cells, inhibition of Na+,K+-ATPase activity is not caused by reduction of speed of enzyme work, but by increase of affinity of ouabainsensitive Na+,K+-ATPase to ATP. At the same time, the Mg2+-binding center of Na+,K+-ATPase in patients with RеA is endogenous. We also found that affinity Na+,K+-ATPase to the ions K+ in peripheral blood lymphocytes of healthy donors is 2.4 times higher than in patients with RеA. We observed that Na+,K+-ATPase of peripheral blood lymphocytes of patients with RеA retains its endogenous receptor properties – sensitivity to ouabain does not change. It is assumed that under conditions of rheumatic pathology the impact on the Na+,K+-ATPase structure occurs both externally and on the cytoplasmic membrane surface. The above experimental data can be used for further clarification of the membrane mechanisms of ion exchange in immunocompetent cells of patients suffering from autoimmune diseases.

scholarly journals Association between inherited thrombophilia in pregnancy and micronucleus frequency in peripheral blood lymphocytes

2017 ◽  
Vol 20 (2) ◽  
pp. 11-18 ◽  
Author(s):  
GM Šošić ◽  
N Jović ◽  
B Rakić ◽  
A Dimitrijević ◽  
M Varjačić
Keyword(s):  
Pregnant Women ◽  
Peripheral Blood ◽  
First Trimester ◽  
Peripheral Blood Lymphocytes ◽  
Control Group ◽  
Case Group ◽  
Endogenous Factors ◽  
Blood Lymphocytes ◽  
The Individual ◽  
The Impact

Abstract The aim of this study was to determine possible predictors of an increased frequency of micronucleus (MN) and the impact of thrombophilia on the chromosomal instability in peripheral blood lymphocytes (PBL) of pregnant women in their first trimester. This study was designed as a case-control study on 74 pregnant women. It was performed in the gestational age of 11 to 14 weeks, when blood samples were collected and incubated for 72 hours. The individual MN frequency in PBL was measured by cytokinesis-block micronucleus (CBMN) assay. Women were grouped in control group [≤4 MN/1000 binucleated (BN) cells] and case group (>4 MN/1000 BN cells). Potential mutagenic effects of exogenous/endogenous factors in pregnant women were analyzed. By analyzing the given results, it can be concluded that pregnant women with thrombophilia have 26.69-times more chance of having a frequency of >4 MN/1000 BN than pregnant women with no thrombophilia. Our research was primarily aimed at showing that the presence of thrombophilia was a statistically important predictor of an increased MN frequency in pregnant women and it can predict about one-third of the total variance in MN frequency in the studied population.

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