Toll-like receptor (TLR9) -1486 T/C (rs187084) gene polymorphism in Egyptian patients with rheumatoid arthritis

AIM: This study aimed to investigate genotype and allele frequencies of −174 (rs1800795) and −572 (rs1800796) IL-6 promoter gene polymorphisms in Egyptian patients with rheumatoid arthritis (RA) in comparison to control group.METHODS: The study was conducted on 198 Egyptian subjects (99 RA patients and 99 healthy control).The promoter region of the IL-6 gene was amplified by PCR using DNAs from patients and the controls, and their PCR products were digested by suitable enzymes.RESULTS: No statistical differences were found in −572G/C genotype (P = 0.177) or allele (P = 0.147) frequencies between RA patients and controls. Significant differences were observed in −174G/C genotype (P ˂ 0.001) and allele (P ˂ 0.001) frequencies between RA patients and controls.CONCLUSION: A significant association of IL-6 −174G/C gene polymorphism and RA in Egyptian population was found with significantly higher frequencies of GC and CC genotypes and C allele in RA patients compared to controls. No association was found between IL-6 −572G/C gene polymorphism and RA.

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Genetic influence of DNAM-1 (DNAX accessory molecule-1) gene polymorphism (rs 763361) on susceptibility and disease activity of systemic lupus erythematosus and rheumatoid arthritis in Egyptian patients

Comparative Clinical Pathology ◽

10.1007/s00580-017-2406-6 ◽

2017 ◽

Vol 26 (3) ◽

pp. 543-552 ◽

Cited By ~ 3

Author(s):

Rabab A. Mohamed ◽

Mervat I. Abd Elazeem

Keyword(s):

Rheumatoid Arthritis ◽

Systemic Lupus Erythematosus ◽

Gene Polymorphism ◽

Disease Activity ◽

Lupus Erythematosus ◽

Genetic Influence ◽

Systemic Lupus ◽

Egyptian Patients ◽

Accessory Molecule

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Faculty Opinions recommendation of Toll-like receptor 2 (TLR2) induces migration and invasive mechanisms in rheumatoid arthritis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.725545334.793510053 ◽

2015 ◽

Author(s):

Ulf Müller-Ladner ◽

Klaus Frommer ◽

Elena Neumann

Keyword(s):

Rheumatoid Arthritis ◽

Toll Like Receptor ◽

Toll Like Receptor 2

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Study of Toll-like Receptor 3 Gene Polymorphism as a Novel Risk Factor for HCV-related Hepatocellular Carcinoma in Egypt

Current Cancer Drug Targets ◽

10.2174/1568009620666200319102929 ◽

2020 ◽

Vol 20 (5) ◽

pp. 382-389 ◽

Cited By ~ 1

Author(s):

Shimaa EL-Sharawy ◽

Osama El- Sayed Negm ◽

Sherief Abd-Elsalam ◽

Hesham Ahmed EL-Sorogy ◽

Mona Ahmed Helmy Shehata

Keyword(s):

Hepatocellular Carcinoma ◽

Risk Factor ◽

Gene Polymorphism ◽

Clinical Laboratory ◽

Treatment Options ◽

Laboratory Evaluation ◽

Toll Like Receptor ◽

Focal Lesions ◽

Tlr3 Gene ◽

Cirrhotic Patients

Background & Aims: Hepatocellular carcinoma (HCC) is a highly aggressive cancer with few treatment options. Toll-like receptor 3 (TLR3) plays a key role in innate immunity and may affect the development of cancers. This study aimed to investigate the association between TLR3 gene polymorphism and HCV-related hepatocellular carcinoma in Egypt. Methods: This work was conducted on 70 individuals; fifty HCV cirrhotic patients were included in two groups; with HCC (30 patients) and without HCC (20 patients) compared with a group of 20 apparently healthy controls. All of the studied individuals underwent clinical-laboratory evaluation. TLR3 gene single-nucleotide polymorphism (SNP) (+1234C/T) was tested by polymerase chain reaction- restriction fragment length polymorphism. Results: This study reported that the prevalence of TLR3 +1234TT genotype was significantly increased in cirrhotic patients with HCC than without HCC, while it was not detected at all among the controls. When analyzing the TLR3 SNP +1234C/T with different clinical parameters in HCC patients, there was a significant association between+1234C/T SNP; namely TT genotype and each of the hepatic focal lesions᾽ number, size and the patients᾽ higher Okuda and BCLC stages. No association could be detected between TLR3 SNP and the age, sex, Child-Pugh grades, MELD score or AFP of the studied HCC cases. Conclusion: TLR3 gene SN P +1234C/T could be a novel risk factor for the HCV-related HCC among the Egyptian population.

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Circadian Rhythms of Endothelial Nitric Oxide Synthase and Toll-like Receptors 2 Production in Females with Rheumatoid Arthritis Depending on NOS3 Gene Polymorphism

Reviews on Recent Clinical Trials ◽

10.2174/1574887115666200416143512 ◽

2020 ◽

Vol 15 (2) ◽

pp. 145-151

Author(s):

Kateryna Zaichko ◽

Nataliia Zaichko ◽

Oleksandr Maievskyi ◽

Oleksandr Korotkyi ◽

Tetyana Falalyeyeva ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Circadian Rhythms ◽

Gene Polymorphism ◽

Endothelial Nitric Oxide Synthase ◽

Toll Like Receptors ◽

Nos3 Gene ◽

Oxide Synthase ◽

Endothelial Nitric Oxide

Background: Rheumatoid Arthritis (RA) is an autoimmune polygenic disease characterized by rapid disability progression and high prevalence. Progression of RA is closely associated with chronobiological changes in the production of some hormones and inflammatory mediators, influencing the disease course and therapy efficacy. The main pathogenetic mechanism of RA is angiogenesis, which is controlled by biological clock-genes. Further investigation of circadian rhythms of angiogenic mediators production in RA patients may be considered as important and relevant. The aim of this study was to establish daily variability of serum endothelial Nitric Oxide Synthase (NOS3) and toll-like receptors 2 (sTLR2) levels in female RA patients depending on the NOS3 gene polymorphism. Methods: We examined 173 RA patients (100% female) aged 43.7 ± 7.35 years and 34 age-matched healthy women without joint diseases and autoimmune diseases (control). RA was diagnosed by ACR/EULAR 2010 criteria. Blood serum NOS3 and sTLR2 levels were determined at 08:00 and 20:00 using Cloud-Clone Corp kits (USA). NOS3 T-786С (rs2070744) polymorphism was determined by Real-Time PCR (Bio-Rad iCycler IQ5) using SNP-express kits. The SPSS22 software package was used for statistical processing of the results. Results: Females with RA demonstrated oppositely directed serum NOS3 and sTLR2 daily changes: NOS3 level in the morning (08:00) was lower than in the evening (+ 45.5 ± 30.7%), and sTLR2 level in the evening (at 20:00) was lower than in the morning (-21.6 ± 13.1%). RA patients had differences in NOS3 and sTLR2 production depending on NOS3 T786C genotype. CC subjects had NOS3 level at 08:00, 20:00 and day average levels lower (16-25%), and sTLR2 level higher (24-27%) than those of TT subjects. RA patients, carriers of CC genotype, had higher chances of NOS3 and sTLR2 aberrant production compared to TT and TC genotype carriers (OR = 2.99 and 4.79, respectively). Conclusion: RA patients demonstrated oppositely directed circadian changes of serum NOS3 and sTLR2. CC genotype carriers had lower NOS3 and higher sTLR2 production rates than TT and TC genotype carriers.

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