Abstract
Peanut butter extracts and samples spiked with 5-40 μg anatoxin B1/kg were analyzed, together with naturally contaminated peanut products, by 3 extraction procedures: the official Dutch method (KB), he Liem et al. method (methanol), and the IUPAC method. The last procedure was selected as a reference method, since it has international application. KB extracts were separated on silica gel G plates with a mixture of chloroform-acetone (90 + 10), whereas IUPAC extracts were separated similarly on MN-G-HR plates. Methanol extracts were resolved on silica gel II plates, using chloroform-trichloroethylene-n-amyl alcohol-formic acid (80 + 15 + 4 + 1) as the developing solvent. After development, plates were scanned with a reflectance flying-spot densitometer. With such techniques, average recoveries for spiked peanut butter extracts ranged from 99 to 105%, with variation values of 11-12%. Recovery values of 69% (KB method) and 84% (methanol and IUPAC methods) were obtained for spiked peanut butter samples. Coefficients of variation ranged from 13 to 15% for fluorodensitometric measurements. Innaturally contaminated peanuts and peanut products , precision values were 13.6% for fluorodensitometric measurements compared to 36% for visual estimations . Both the methanol and IUPAC methods yield extracts suitable for densitometric analysis after spotting on TLC plates; the analytical results obtained are comparable. Extracts from the KB method contained more interfering fluorescent material than the other 2 methods