Lipase from the thermotolerant fungus Rhizopus homothallicus is more thermostable when produced using solid state fermentation than liquid fermentation procedures

The purpose of this work is to study the optimized cultural conditions for the production of amylase by Aspergillus niger in solid state and submerged liquid fermentation. Four solid substrates banana peel, corn, potato and tapioca with different moisture conditions were taken for solid state fermentation (SSF). Basal medium was used for submerged liquid fermentation (SLF) with different pH (3 to 8), temperature (25, 30, 35 and 40ºC), carbon concentration (1, 2 and 3 g) and nitrogen source (0.1, 0.2 and 0.3 g). In SSF, tapioca yielded highest amylase activity and specific activity (4.43U/ml and 4.58U/mg) at 50% moisture content. In SLF, 2 g starch and 0.3 g peptone concentration showed 0.78 and 1.23 U/ml amylase activities under the optimum pH (5) and temperature (30ºC) the amylase activities reached to 0.86 U/ml and 0.76 U/ml respectively. In SSF using tapioca as substrate the enzyme yield is about five times higher than that achieved with submerged liquid culture. DOI: http://dx.doi.org/10.3329/bjsir.v47i1.7310 Bangladesh J. Sci. Ind. Res. 47(1), 99-104, 2012

Download Full-text

Characterization of best naringinase producing fungus strain isolated from palmyrah (Borrasus flabellifer) fruit pulp

International Journal of Biological Research ◽

10.14419/ijbr.v4i2.6289 ◽

2016 ◽

Vol 4 (2) ◽

pp. 97

Author(s):

Sinthuja Karuppaija ◽

Kapilan Ranganathan ◽

Vasantharuba Seevaratnam

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Large Scale ◽

Agricultural Waste ◽

Selective Medium ◽

Fruit Pulp ◽

Fermentation System ◽

Fungal Strains ◽

Liquid Fermentation ◽

Fermentation Media

Background: The Palmyrah (Borrasus flabellifer L.) fruit pulp has the bitter compound flabelliferin (a tetraglycoside) which can be hydrolyzed by naringinase enzyme. The diverse groups of filamentous fungi and bacteria that live in different substrates have the capacity of producing extracellular naringinase enzyme which is of tremendous industrial value.Objective: The objective of the study was to isolate the naringinase producing fungal strains from Palmyrah and to identify the best naringinase producer under liquid and solid state fermentation systems.Methods: Fungal strains isolated from Palmyrah fruit pulp and the soil where pulp is allowed to decay, were grown on naringin agar selective medium at pH 6.0 at room temperature and the production of extracellular naringinase was measured in the liquid fermentation media and solid state fermentation system using paddy husk as support.Results: Five fungal strains isolated from the palmyrah pulp and the pulp decaying in sand designated as PF1,PF2,PF3,PF4 & PF5 had the ability to produce extracellular naringinase enzyme in liquid fermentation media. Fungal strain PF4 that showed highest naringinase enzyme activity (1.769U/ml) was selected among the isolated five fungal strains and identified as Rhizophus stolonifer based on the morphological and biochemical characteristics. When this strain was grown in the solid state fermentation system using paddy husk as media, narininase production was higher (269.84 U/gram of dry substrate) in seven days.Conclusion: Rhizophus stolonifer could be used to produce large scale naringinase enzyme under solid state fermentation system using very cheap, easily available, agricultural waste paddy husk as support without the need of expensive and well equipped laboratories.

Download Full-text

Improving lipase production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation

Process Biochemistry ◽

10.1016/j.procbio.2006.05.017 ◽

2006 ◽

Vol 41 (11) ◽

pp. 2264-2269 ◽

Cited By ~ 76

Author(s):

J.A. Rodriguez ◽

J.C. Mateos ◽

J. Nungaray ◽

V. González ◽

T. Bhagnagar ◽

...

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Lipase Production ◽

Nutrient Source ◽

Rhizopus Homothallicus

Download Full-text

Comparative study on the properties of lipopeptide products and expression of biosynthetic genes from Bacillus amyloliquefaciens XZ-173 in liquid fermentation and solid-state fermentation

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-013-1375-4 ◽

2013 ◽

Vol 29 (11) ◽

pp. 2105-2114 ◽

Cited By ~ 4

Author(s):

Zhen Zhu ◽

Jianchao Zhang ◽

Yanliang Wu ◽

Wei Ran ◽

Qirong Shen

Keyword(s):

Solid State ◽

Comparative Study ◽

Solid State Fermentation ◽

Bacillus Amyloliquefaciens ◽

Biosynthetic Genes ◽

Liquid Fermentation

Download Full-text

Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

BMC Microbiology ◽

10.1186/s12866-021-02093-6 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Ghadir S. El-Housseiny ◽

Asmaa A. Ibrahim ◽

Mahmoud A. Yassien ◽

Khaled M. Aboshanab

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Environmental Conditions ◽

Time Course ◽

Water Discharge ◽

Solid Substrate ◽

Inoculum Size ◽

Streptomyces Rimosus ◽

Promising Alternative ◽

Liquid Fermentation

Abstract Background Paromomycin is a 2-deoxystreptamine aminocyclitol aminoglycoside antibiotic with broad spectrum activity against Gram-negative, Gram-positive bacteria and many protozoa. This study introduces a strategy for paromomycin production through solid-state fermentation using Streptomyces rimosus subsp. paromomycinus NRRL 2455. Solid state fermentation has gained enormous attention in the development of several products because of their numerous advantages over submerged liquid fermentation. After selecting the best solid substrate, a time course study of paromomycin production was carried out followed by optimization of environmental conditions using response surface methodology. Paromomycin yields obtained using this technique were also compared to those obtained using submerged liquid fermentation. Results Upon screening of 6 different substrates, maximum paromomycin concentration (0.51 mg/g initial dry solids) was obtained with the cost-effective agro-industrial byproduct, corn bran, impregnated with aminoglycoside production media. Optimization of environmental conditions using D-optimal design yielded a 4.3-fold enhancement in paromomycin concentration reaching 2.21 mg/g initial dry solids at a pH of 8.5, inoculum size of 5% v/w and a temperature of 30 °C. Conclusion Compared to submerged liquid fermentation, solid state fermentation resulted in comparable paromomycin concentrations, cost reduction of raw materials, less energy consumption and waste water discharge, which have major implications in industrial fermentation. Therefore, solid state fermentation is a promising alternative to submerged liquid fermentation for paromomycin production. To the best of our knowledge, this is the first report on the optimized paromomycin production through solid state fermentation process.

Download Full-text

Production and Statistical Optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in Solid State Fermentation

10.21203/rs.3.rs-100837/v1 ◽

2020 ◽

Author(s):

Ghadir S. El-Housseiny ◽

Asmaa A Ibrahim ◽

Mahmoud A Yassien ◽

Khaled Aboshanab

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Environmental Conditions ◽

Time Course ◽

Water Discharge ◽

Aminoglycoside Antibiotic ◽

Solid Substrate ◽

Inoculum Size ◽

Streptomyces Rimosus ◽

Liquid Fermentation

Abstract Background: Paromomycin is a 2 deoxystreptamine aminocyclitol aminoglycoside antibiotic with broad spectrum activity against Gram-negative, Gram-positive bacteria and many protozoa. This study introduces a strategy for paromomycin production through solid-state fermentation using Streptomyces rimosus subsp. paromomycinus NRRL 2455. Solid state fermentation has gained enormous attention in the development of several products because of their numerous advantages over submerged liquid fermentation. After selecting the best solid substrate, a time course study of paromomycin production was carried out followed by optimization of environmental conditions using response surface methodology. Paromomycin yields obtained using this technique were also compared to those obtained using submerged liquid fermentation.Results: Upon screening of 6 different substrates, maximum paromomycin concentration (0.51 mg/g initial dry solids) was obtained with the cost-effective agro-industrial byproduct, corn bran, impregnated with aminoglycoside production media. This value was higher than that obtained using submerged liquid fermentation using the same conditions. Optimization of environmental conditions using D optimal design yielded a 4.3-fold enhancement in paromomycin concentration reaching 2.21 mg/g initial dry solids at a pH of 8.5, inoculum size of 5% v/w and a temperature of 30 °C. Conclusion: Compared to submerged liquid fermentation, solid state fermentation resulted in higher paromomycin concentrations, cost reduction of raw materials, less energy consumption and waste water discharge, which have major implications in industrial fermentation. Therefore, SSF is a superior alternative to SLF for paromomycin production. To the best of our knowledge, this is the first report on the optimized paromomycin production through solid state fermentation process.

Download Full-text