scholarly journals Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ghadir S. El-Housseiny ◽  
Asmaa A. Ibrahim ◽  
Mahmoud A. Yassien ◽  
Khaled M. Aboshanab
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Environmental Conditions ◽  
Time Course ◽  
Water Discharge ◽  
Solid Substrate ◽  
Inoculum Size ◽  
Streptomyces Rimosus ◽  
Promising Alternative ◽  
Liquid Fermentation

Abstract Background Paromomycin is a 2-deoxystreptamine aminocyclitol aminoglycoside antibiotic with broad spectrum activity against Gram-negative, Gram-positive bacteria and many protozoa. This study introduces a strategy for paromomycin production through solid-state fermentation using Streptomyces rimosus subsp. paromomycinus NRRL 2455. Solid state fermentation has gained enormous attention in the development of several products because of their numerous advantages over submerged liquid fermentation. After selecting the best solid substrate, a time course study of paromomycin production was carried out followed by optimization of environmental conditions using response surface methodology. Paromomycin yields obtained using this technique were also compared to those obtained using submerged liquid fermentation. Results Upon screening of 6 different substrates, maximum paromomycin concentration (0.51 mg/g initial dry solids) was obtained with the cost-effective agro-industrial byproduct, corn bran, impregnated with aminoglycoside production media. Optimization of environmental conditions using D-optimal design yielded a 4.3-fold enhancement in paromomycin concentration reaching 2.21 mg/g initial dry solids at a pH of 8.5, inoculum size of 5% v/w and a temperature of 30 °C. Conclusion Compared to submerged liquid fermentation, solid state fermentation resulted in comparable paromomycin concentrations, cost reduction of raw materials, less energy consumption and waste water discharge, which have major implications in industrial fermentation. Therefore, solid state fermentation is a promising alternative to submerged liquid fermentation for paromomycin production. To the best of our knowledge, this is the first report on the optimized paromomycin production through solid state fermentation process.

scholarly journals Production and Statistical Optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in Solid State Fermentation

2020 ◽  
Author(s):  
Ghadir S. El-Housseiny ◽  
Asmaa A Ibrahim ◽  
Mahmoud A Yassien ◽  
Khaled Aboshanab
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Environmental Conditions ◽  
Time Course ◽  
Water Discharge ◽  
Aminoglycoside Antibiotic ◽  
Solid Substrate ◽  
Inoculum Size ◽  
Streptomyces Rimosus ◽  
Liquid Fermentation

Abstract Background: Paromomycin is a 2 deoxystreptamine aminocyclitol aminoglycoside antibiotic with broad spectrum activity against Gram-negative, Gram-positive bacteria and many protozoa. This study introduces a strategy for paromomycin production through solid-state fermentation using Streptomyces rimosus subsp. paromomycinus NRRL 2455. Solid state fermentation has gained enormous attention in the development of several products because of their numerous advantages over submerged liquid fermentation. After selecting the best solid substrate, a time course study of paromomycin production was carried out followed by optimization of environmental conditions using response surface methodology. Paromomycin yields obtained using this technique were also compared to those obtained using submerged liquid fermentation.Results: Upon screening of 6 different substrates, maximum paromomycin concentration (0.51 mg/g initial dry solids) was obtained with the cost-effective agro-industrial byproduct, corn bran, impregnated with aminoglycoside production media. This value was higher than that obtained using submerged liquid fermentation using the same conditions. Optimization of environmental conditions using D optimal design yielded a 4.3-fold enhancement in paromomycin concentration reaching 2.21 mg/g initial dry solids at a pH of 8.5, inoculum size of 5% v/w and a temperature of 30 °C. Conclusion: Compared to submerged liquid fermentation, solid state fermentation resulted in higher paromomycin concentrations, cost reduction of raw materials, less energy consumption and waste water discharge, which have major implications in industrial fermentation. Therefore, SSF is a superior alternative to SLF for paromomycin production. To the best of our knowledge, this is the first report on the optimized paromomycin production through solid state fermentation process.

scholarly journals Performance of Fungal Growth Through Integrated Gompertz Model and Respiratory Quotient by Solid State Fermentation in Multi-Layer Squared Tray Solid State Bioreactor with Aeration Strategies

2021 ◽  
Author(s):  
Musaalbakri Abdul Manan ◽  
Colin Webb
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Respiratory Quotient ◽  
Fungal Growth ◽  
Gompertz Model ◽  
Solid Substrate ◽  
Aspergillus Awamori ◽  
Promising Alternative ◽  
Fungal Activity ◽  
On Line

Abstract A newly designed, laboratory-scaled and multi-layer squared tray solid state bioreactor (SSB), was developed and successfully operated in solid state fermentation (SSF) conditions. The bioreactor was divided into eight layers of squared perforated trays. Wheat bran was used as a solid substrate for the growth of Aspergillus awamori and Aspergillus oryzae. The SSB was equipped with an oxygen (O2)/carbon dioxide (CO2) gas analyser and a thermocouple. Continuous on-line monitoring of fungal growth could be performed by indirect methods that measure O2 consumed, production of CO2 and metabolic heat. The advantage of using this method is that there are no tedious and time-consuming sampling processes. The evolution of CO2, which represents an accumulation term, was integrated with time and fitted to the Gompertz model in a log-like equation. The Gompertz model generated values that may be used to stimulate and verify the experimental data. Results strongly suggest that the evolved and accumulated CO2, excellently described fungal growth. Simulated results agreed with experimental results. The respiratory quotient (RQ), which is the ratio of CO2 evolution rate (CER) to O2 uptake rate (OUR), was determined by the gas balance method. CER and OUR confirmed that measurements correlated to fungal activity. Each RQ values can explain the differences of each SFF process carried out. Yet, heat evolved by fungal activity also described fungal growth. The current findings is an excellent pre-liminary experimental work, evidencing that multi-layer squared tray SSB with forced moistened aeration present a promising alternative of instrumented bioreactors for SSF processes.

scholarly journals OPTIMISASI PRODUKSI ENZIM LAKASE PADA FERMENTASI KULTUR PADAT MENGGUNAKAN JAMUR PELAPUK PUTIH Marasmius sp. : PENGARUH UKURAN PARTIKEL, KELEMBAPAN, DAN KONSENTRASI Cu

2016 ◽  
Vol 3 (02) ◽  
Author(s):  
Cornelius Damar Hanung ◽  
Ronald Osmond ◽  
Hendro Risdianto ◽  
Sri Harjati Suhardi ◽  
Tjandra Setiadi
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Moisture Content ◽  
Rice Straw ◽  
Solid State Fermentation ◽  
Carbon Sources ◽  
White Rot Fungi ◽  
Batch Process ◽  
Solid Substrate ◽  
White Rot

White rot fungi of Marasmius sp. is a fungus which produce laccase in high activity. Laccase is one of the ligninolityc enzymes that capable to degrade lignin. This ability can be used for the pretreatment of lignocellulosic materials in the bioethanol production. Laccase was produced in flask by batch process using Solid State Fermentation (SSF). The optimisation was conducted by statistically of full factorial design. The particle size, moisture content, and Cu concentration were investigated in this study. Rice straw was used as solid substrate and the glycerol was used as the carbon sources in modified Kirk medium. The results showed that particle size of rice straw did not affect significantly to the enzyme activity. The highest laccase activity of 4.45 IU/g dry weight was obtained at the moisture content of 61% and Cu concentration of 0.1 mM.Keywords: laccase, Marasmius sp., optimisation, rice straw, solid state fermentation ABSTRAKJamur pelapuk putih, Marasmius sp. merupakan jamur yang menghasilkan enzim lakase dengan aktivitas tinggi. Lakase merupakan enzim ligninolitik yang dapat mendegradasi lignin. Kemampuan ini dapat digunakan untuk proses pengolahan awal bahan lignoselulosa pada pembuatan bioetanol. Produksi lakase dilakukan dalam labu dengan modus batch menggunakan fermentasi kultur padat. Optimisasi produksi enzim lakase dengan metode fermentasi padat dilakukan dengan  rancangan percobaan faktorial penuh. Pengaruh ukuran partikel, kelembapan, dan konsentrasi Cu diuji dengan medium penyangga jerami dengan menambahkan gliserol dalam medium Kirk termodifikasi sebagai sumber karbon. Penelitian ini menunjukkan bahwa ukuran jerami tidak berpengaruh signifikan terhadap aktivitas enzim. Aktivitas enzim lakase maksimum terjadi pada saat kelembapan 61% dan konsentrasi Cu 0,1 mM dengan aktivitas enzim lakase/berat kering tertinggi mencapai 4,45 IU/g.Kata kunci: lakase, Marasmius sp., optimisasi, jerami, fermentasi kultur padat

scholarly journals Amylase Production from Solid State Fermentation and Submerged Liquid Fermentation by Aspergillus niger

2012 ◽  
Vol 47 (1) ◽  
pp. 99-104 ◽  
Author(s):  
G Dharani ◽  
NS Kumaran
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Specific Activity ◽  
Basal Medium ◽  
Banana Peel ◽  
Liquid Fermentation ◽  
Cultural Conditions ◽  
Solid Substrates ◽  
Moisture Conditions

The purpose of this work is to study the optimized cultural conditions for the production of amylase by Aspergillus niger in solid state and submerged liquid fermentation. Four solid substrates banana peel, corn, potato and tapioca with different moisture conditions were taken for solid state fermentation (SSF). Basal medium was used for submerged liquid fermentation (SLF) with different pH (3 to 8), temperature (25, 30, 35 and 40ºC), carbon concentration (1, 2 and 3 g) and nitrogen source (0.1, 0.2 and 0.3 g). In SSF, tapioca yielded highest amylase activity and specific activity (4.43U/ml and 4.58U/mg) at 50% moisture content. In SLF, 2 g starch and 0.3 g peptone concentration showed 0.78 and 1.23 U/ml amylase activities under the optimum pH (5) and temperature (30ºC) the amylase activities reached to 0.86 U/ml and 0.76 U/ml respectively. In SSF using tapioca as substrate the enzyme yield is about five times higher than that achieved with submerged liquid culture. DOI: http://dx.doi.org/10.3329/bjsir.v47i1.7310 Bangladesh J. Sci. Ind. Res. 47(1), 99-104, 2012

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