Measurements of genotoxic potential of cadmium in different tissues of fresh water climbing perch Anabas testudineus (Bloch), using the comet assay

AbstractDiseased Anabas testudineus exhibiting signs of tail-rot and ulcerations on body were collected from a fish farm in Assam, India during the winter season (November 2018 to January 2019). Swabs from the infected body parts were streaked on sterilized nutrient agar. Two dominant bacterial colonies were obtained, which were then isolated and labelled as AM-31 and AM-05. Standard biochemical characterisation and 16S rRNA and rpoB gene sequencing identified AM-31 isolate as Aeromonas hydrophila and AM-05 as Aeromonas jandaei. Symptoms similar to that of natural infection were observed on re-infecting both bacteria to disease-free A. testudineus, which confirmed their virulence. LC50 was determined at 1.3 × 104 (A. hydrophila) and 2.5 × 104 (A. jandaei) CFU per fish in intraperitoneal injection. Further, PCR amplification of specific genes responsible for virulence (aerolysin and enterotoxin) confirmed pathogenicity of both bacteria. Histopathology of kidney and liver in the experimentally-infected fishes revealed haemorrhage, tubular degeneration and vacuolation. Antibiotic profiles were also assessed for both bacteria. To the best of our knowledge, the present work is a first report on the mortality of farmed climbing perch naturally-infected by A. hydrophila as well as A. jandaei, with no records of pathogenicity of the latter in this fish.

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Comet assay evaluation of six chemicals of known genotoxic potential in rats

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/j.mrgentox.2015.03.003 ◽

2015 ◽

Vol 786-788 ◽

pp. 172-181 ◽

Cited By ~ 8

Author(s):

Cheryl A. Hobbs ◽

Leslie Recio ◽

Michael Streicker ◽

Molly H. Boyle ◽

Jin Tanaka ◽

...

Keyword(s):

Comet Assay ◽

Genotoxic Potential

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Sensory cues employed for the acquisition of familiarity-dependent recognition of a shoal of conspecifics by climbing perch (Anabas testudineus Bloch)

Journal of Biosciences ◽

10.1007/s12038-015-9529-1 ◽

2015 ◽

Vol 40 (2) ◽

pp. 225-232 ◽

Cited By ~ 3

Author(s):

VV Binoy ◽

Rajesh Kasturirangan ◽

Anindya Sinha

Keyword(s):

Sensory Cues ◽

Anabas Testudineus ◽

Climbing Perch

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Growth and survival of a diallel cross for five strains of climbing perch (Anabas testudineus Bloch, 1792) in Thailand

Agriculture and Natural Resources ◽

10.1016/j.anres.2016.05.001 ◽

2016 ◽

Vol 50 (5) ◽

pp. 351-356

Author(s):

Nitikorn Piwpong ◽

Jarunee Chiayvareesajja ◽

Sommai Chiayvareesajja

Keyword(s):

Diallel Cross ◽

Anabas Testudineus ◽

Growth And Survival ◽

Climbing Perch

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Pengaruh C/N Rasio Berbeda untuk Pembentukan Bioflok pada Media Pemeliharaan terhadap Pertumbuhan dan Kelangsungan Hidup Benih Ikan Betok (Anabas testudineus)

Jurnal Lahan Suboptimal ◽

10.33230/jlso.7.1.2018.349 ◽

2019 ◽

Vol 7 (1) ◽

Author(s):

M. Indra Wahyu Pratama ◽

Dade Jubaedah ◽

Mohamad Amin

Keyword(s):

Survival Rate ◽

Culture Media ◽

Farming System ◽

Anabas Testudineus ◽

Growth And Survival ◽

Climbing Perch ◽

Randomized Design ◽

Absolute Growth ◽

Biofloc Technology ◽

Absolute Length

Pratama et al, 2018. The Effect of Different C/N Ratio in for Biofloc of Formation Culture Media to the Growth and Survival Rate of Juvenile Climbing Perch (Anabas testudineus). JLSO 7(1): Climbing perch fish resources still have a problem due to this fish is still obtained only through capture and also the growth of climbing perch fish is slow. The solutions to increase the growth of the climbing perch fish is by applying biofloc technology for climbing perch fish culture. The purpose of this research was to know the effect of different C/N ratio on the growth of climbing perch fish (Anabas testudineus) juvenille with biofloc farming system. This research used a completely randomized design consist of four treatments and three replications. The treatments were without molase (P0), molase with C/N ratio 15 (P1), C/N 20 (P2) and C/N ratio 25 (P3). The results showed that C / N ratio 20 was the best treatment with absolute length of growth of 1.33 cm, absolute growth of weight 1.92 g, survival rate of 99.17% and feed efficiency 138.06%.

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