scholarly journals Systematic Identification of MicroRNAs That Impact on Proliferation of Prostate Cancer Cells and Display Changed Expression in Tumor Tissue

2016 ◽  
Vol 69 (6) ◽  
pp. 1120-1128 ◽  
Author(s):  
Anna Aakula ◽  
Pekka Kohonen ◽  
Suvi-Katri Leivonen ◽  
Rami Mäkelä ◽  
Petteri Hintsanen ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Tumor Tissue ◽  
Prostate Cancer Cells ◽  
Systematic Identification

scholarly journals SULT2B1b Sulfotransferase: Induction by Vitamin D Receptor and Reduced Expression in Prostate Cancer

2013 ◽  
Vol 27 (6) ◽  
pp. 925-939 ◽  
Author(s):  
Young-Kyo Seo ◽  
Nooshin Mirkheshti ◽  
Chung S. Song ◽  
Soyoung Kim ◽  
Sherry Dodds ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Vitamin D ◽  
Cancer Cells ◽  
Vitamin D Receptor ◽  
Tumor Tissue ◽  
Tissue Array ◽  
Cancer Tissue ◽  
Prostate Cancer Cells ◽  
Androgen Synthesis ◽  
Mouse Prostate

Abstract An elevated tumor tissue androgen level, which reactivates androgen receptor in recurrent prostate cancer, arises from the intratumor synthesis of 5α-dihydrotestosterone through use of the precursor steroid dehydroepiandrosterone (DHEA) and is fueled by the steroidogenic enzymes 3β-hydroxysteroid dehydrogenase (3β-HSD1), aldoketoreductase (AKR1C3), and steroid 5-alpha reductase, type 1 (SRD5A1) present in cancer tissue. Sulfotransferase 2B1b (SULT2B1b) (in short, SULT2B) is a prostate-expressed hydroxysteroid SULT that converts cholesterol, oxysterols, and DHEA to 3β-sulfates. DHEA metabolism involving sulfonation by SULT2B can potentially interfere with intraprostate androgen synthesis due to reduction of free DHEA pool and, thus, conversion of DHEA to androstenedione. Here we report that in prostatectomy specimens from treatment-naive patients, SULT2B expression is markedly reduced in malignant tissue (P < .001, Mann-Whitney U test) compared with robust expression in adjacent nonmalignant glands. SULT2B was detected in formalin-fixed specimens by immunohistochemistry on individual sections and tissue array. Immunoblotting of protein lysates of frozen cancer and matched benign tissue confirmed immunohistochemistry results. An in-house–developed rabbit polyclonal antibody against full-length human SULT2B was validated for specificity and used in the analyses. Ligand-activated vitamin D receptor induced the SULT2B1 promoter in vivo in mouse prostate and increased SULT2B mRNA and protein levels in vitro in prostate cancer cells. A vitamin D receptor/retinoid X receptor-α–bound DNA element (with a DR7 motif) mediated induction of the transfected SULT2B1 promoter in calcitriol-treated cells. SULT2B knockdown caused an increased proliferation rate of prostate cancer cells upon stimulation by DHEA. These results suggest that the tumor tissue SULT2B level may partly control prostate cancer growth, and its induction in a therapeutic setting may inhibit disease progression.

279: Inhibition of Cyclooxygenase-2 Pathway Overcomes the Heat-Resistance in Prostate Cancer Cells

2007 ◽  
Vol 177 (4S) ◽  
pp. 93-93
Author(s):  
Makoto Sumitomo ◽  
Kenji Kuroda ◽  
Takako Asano ◽  
Akio Horiguchi ◽  
Keiichi Ito ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Heat Resistance ◽  
Cancer Cells ◽  
Cyclooxygenase 2 ◽  
Prostate Cancer Cells

574: Cellular Interaction Between Prostate Cancer and Bone Stroma: Effects of Radiation on the Growth and Colonization of Human Prostate Cancer Cells in Bone

2005 ◽  
Vol 173 (4S) ◽  
pp. 157-157
Author(s):  
Tohru Miyagi ◽  
Cynthia S. Anderson ◽  
Valerie A. Odero-Marah ◽  
Peter Johnstone ◽  
Leland W.K. Chung
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Human Prostate ◽  
Cellular Interaction ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Effects Of Radiation

430: Modulation of Heat-Shock Protein 90 (HSP90) Client Protein Expression in Prostate Cancer Cells by a Novel Novobiocin Analog

2006 ◽  
Vol 175 (4S) ◽  
pp. 140-140
Author(s):  
Manlio A. Goetzl ◽  
Brian S. Blagg ◽  
Benjamin Cronk ◽  
Len Neckers ◽  
Jeffrey M. Holzbeierlein
Keyword(s):  
Prostate Cancer ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Cancer Cells ◽  
Heat Shock Protein 90 ◽  
Client Protein ◽  
Prostate Cancer Cells ◽  
Hsp90 Client Protein

609: Effects of Sirna and Phytoestrogen Treatments on the Expression of Genes Regulated through the Androgen Receptor of Lncap Prostate Cancer Cells

2004 ◽  
Vol 171 (4S) ◽  
pp. 162-162
Author(s):  
Paul Thelen ◽  
Michal Grzmil ◽  
Iris E. Eder ◽  
Barbara Spengler ◽  
Peter Burfeind ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Expression Of Genes

Pharmacoproteomic and toxicoproteomic study of the natural product Ebenfuran III in DU-145 prostate cancer cells using iTRAQ with 2D LC and tandem mass spectrometry

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
M Halabalaki ◽  
IT Roumeliotis ◽  
E Giannopoulou ◽  
X Alexi ◽  
L Meijer ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Cancer Cells ◽  
Natural Product ◽  
Tandem Mass ◽  
Prostate Cancer Cells
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