Circular RNA HIPK3 regulates human lens epithelial cell dysfunction by targeting the miR-221–3p/PI3K/AKT pathway in age-related cataract

2020 ◽  
Vol 198 ◽  
pp. 108128 ◽  
Author(s):  
Gangfeng Cui ◽  
Ledan Wang ◽  
Wenjuan Huang
Keyword(s):  
Epithelial Cell ◽  
Circular Rna ◽  
Lens Epithelial Cell ◽  
Human Lens ◽  
Akt Pathway ◽  
Human Lens Epithelial Cell ◽  
Cell Dysfunction ◽  
Age Related

scholarly journals SUMOylation Evoked by Oxidative Stress Reduced Lens Epithelial Cell Antioxidant Functions by Increasing the Stability and Transcription of TP53INP1 in Age-Related Cataracts

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Bo Lu ◽  
Ian T. Christensen ◽  
Tao Yu ◽  
Chunxia Wang ◽  
Qichang Yan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Epithelial Cell ◽  
Cell Injury ◽  
Lens Epithelial Cell ◽  
Human Lens ◽  
Human Lens Epithelial Cell ◽  
Age Related ◽  
The Stability

Oxidative stress plays an important role in the pathogenesis of cataracts. Small ubiquitin-like modifier (SUMO) proteins have great effects on cell stress response. Previous studies have shown that TP53INP1 can arrest cell growth and induce apoptosis by modulating p53 transcriptional activity and that both TP53INP1 and p53 are substrates of SUMOylation. However, no previous research has studied the effect of SUMOylation on the oxidative stress response in cataracts. This is the first study to investigate the effect of SUMOylation of TP53INP1 in oxidative stress-induced lens epithelial cell injury and age-related cataract formation. We found that the oxidative stress-induced endogenous SUMOylation of TP53INP1 promoted human lens epithelial cell (holed) apoptosis and regulated hLEC antioxidant effects by increasing the stability and transcription of TP53INP1 in age-related cataracts. SUMO-1, SUMOylation, and TP53INP1 were upregulated in lens tissues affected by age-related cataracts. A SUMO-1-specific protease, SENP1, acted as an oxidative stress-sensitive target gene in hLECs. This study identified for the first time that TP53INP1 can be SUMOylated in vivo, that the SUMOylation of TP53INP1 is induced by oxidative stress, and that SUMOylation/deSUMOylation can affect the stability and transcription of TP53INP1 in hLECs.

EAAT and Xc−Exchanger Inhibition Depletes Glutathione in the Transformed Human Lens Epithelial Cell Line SRA 01/04

2015 ◽  
pp. 1-10 ◽  
Author(s):  
Marlyn P. Langford ◽  
Thomas B. Redens ◽  
Chanping Liang ◽  
A. Scott Kavanaugh ◽  
Donald E. Texada
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Lens Epithelial Cell ◽  
Human Lens ◽  
Epithelial Cell Line ◽  
Human Lens Epithelial Cell

scholarly journals Ferulic Acid Suppresses AmyloidβProduction in the Human Lens Epithelial Cell Stimulated with Hydrogen Peroxide

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Noriaki Nagai ◽  
Sachiyo Kotani ◽  
Yu Mano ◽  
Akina Ueno ◽  
Yoshimasa Ito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ferulic Acid ◽  
Lens Epithelial Cell ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Rt Pcr ◽  
Human Lens Epithelial Cell ◽  
Oxidative Stresses ◽  
Age Related ◽  
The Brain

It is well known that oxidative stresses induce the production of amyloidβ(Aβ) in the brain, lens, and retina, leading to age-related diseases. In the present study, we investigated the effects of ferulic acid on the Aβlevels in H2O2-stimulated human lens epithelial (HLE) SRA 01/04 cells. Three types of Aβpeptides (Aβ1-40, Aβ1-42, and Aβ1-43) were measured by ELISA, and the levels of mRNA for the expressed proteins related to Aβproduction (APP, BACE1, and PS proteins) and degradation (ADAM10, NEP, and ECE1 proteins) were determined by quantitative real-time RT-PCR. H2O2stimulation augmented gene expression of the proteins related to Aβproduction, resulting in the production of three types of Aβpeptides. Treatment with 0.1 μM ferulic acid attenuated the augmentations of gene expression and production of the proteins related to the secretion of three types of Aβpeptides in the H2O2-stimulated HLE cells. These results provided evidence of antioxidative functions of ferulic acid for lens epithelial cells.

Effect of growth factors on proliferation and expression of growth factor receptors in a human lens epithelial cell line

2006 ◽  
Vol 32 (3) ◽  
pp. 510-514 ◽  
Author(s):  
Juergen Kampmeier ◽  
Alicja Baldysiak-Figiel ◽  
Yvonne de Jong-Hesse ◽  
Gerhard K. Lang ◽  
Gabriele E. Lang
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Epithelial Cell ◽  
Cell Line ◽  
Growth Factor Receptors ◽  
Lens Epithelial Cell ◽  
Human Lens ◽  
Epithelial Cell Line ◽  
Human Lens Epithelial Cell
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