Ultrasound-assisted natural deep eutectic solvent extraction of anthocyanin from black carrots: optimization, cytotoxicity, in-vitro bioavailability and stability

2022 ◽  
Author(s):  
Duygu Aslan Türker ◽  
Mahmut Doğan
Keyword(s):  
Solvent Extraction ◽  
Deep Eutectic Solvent ◽  
Ultrasound Assisted ◽  
Natural Deep Eutectic Solvent

scholarly journals Ultrasound-Assisted Deep Eutectic Solvent Extraction of Anthocyanins from Blueberry Wine Residues: Optimization, Identification, and HepG2 Antitumor Activity

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5456
Author(s):  
Hongkun Xue ◽  
Jiaqi Tan ◽  
Qian Li ◽  
Jintian Tang ◽  
Xu Cai
Keyword(s):  
Solvent Extraction ◽  
Antitumor Activity ◽  
Deep Eutectic Solvent ◽  
Extraction Process ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Brewing Process ◽  
Ultrasound Assisted ◽  
Optimum Extraction ◽  
Better Than

Blueberry wine residues produced during the wine-brewing process contain abundant anthocyanins and other bioactive compounds. To extract anthocyanins from blueberry wine residues more efficiently, a novel procedure of ultrasound-assisted deep eutectic solvent extraction (UADESE) was proposed in this work. The extraction process was optimized by response surface methodology coupled with genetic algorithm. The optimum extraction parameters to achieve the highest yield of anthocyanins (9.32 ± 0.08 mg/g) from blueberry wine residues by UADESE were obtained at water content of 29%, ultrasonic power of 380 W, extraction temperature of 55 °C, and extraction time of 40 min. The AB-8 macroporous resin combined with Sephadex LH-20 techniques was used to purify the crude extract (CE) obtained under optimum extraction conditions and analyze the anthocyanins composition by HPLC-ESI-MS/MS. The cyanidin-3-rutinoside with purity of 92.81% was obtained. The HepG2 antitumor activity of CE was better than that of the purified anthocyanins component. Moreover, CE could increase the intracellular reactive oxygen species levels and the apoptosis, and arrest HepG2 cells in the S phases. These findings provided an effective and feasible method for anthocyanins extraction, and reduced the environmental burden of this waste.

Ultrasound-assisted deep eutectic solvent extraction of echinacoside and oleuropein from Syringa pubescens Turcz.

2020 ◽  
Vol 151 ◽  
pp. 112442 ◽  
Author(s):  
Xinsheng Wang ◽  
Yanfang Wu ◽  
Jie Li ◽  
Aoxiang Wang ◽  
Guangyao Li ◽  
...  
Keyword(s):  
Solvent Extraction ◽  
Deep Eutectic Solvent ◽  
Ultrasound Assisted

scholarly journals Resveratrol-Linoleate protects from exacerbated endothelial permeability via a drastic inhibition of the MMP-9 activity

2018 ◽  
Vol 38 (4) ◽  
Author(s):  
Aly Shamseddin ◽  
Céline Crauste ◽  
Erwan Durand ◽  
Pierre Villeneuve ◽  
Grégor Dubois ◽  
...  
Keyword(s):  
Water Solubility ◽  
Critical Role ◽  
Deep Eutectic Solvent ◽  
Endothelial Permeability ◽  
Platelet Endothelial Cell Adhesion ◽  
Lipid Derivative ◽  
Integrity Protection ◽  
Natural Deep Eutectic Solvent

Gelatinolytic matrix metalloproteinases (MMP-2, -9) play a critical role not only in mammals physiology but also during inflammation and healing processes. The natural stilbenoid, resveratrol (RES), exhibits potent antioxidant effects, in a hormetic mode of action, and is known to inhibit MMP-9. However, RES administration exhibits major issues, including poor bioavailability and water solubility, hampering its potential therapeutic effect in vivo. In the present study, we synthesized and evaluated five novel RES–lipid conjugates to increase their cell membrane penetration and improve their bioavailability. The best in vitro MMP-9 inhibitory activity of RES–lipids conjugates was observed with RES-linoleic acid (LA) (5 µM), when dissolved in a natural deep eutectic solvent (NADES), composed of an equimolar content of 1,2-propanediol:choline chloride (ChCl):water. The inhibition of MMP-9 expression by RES-LA in activated THP-1 monocytes, was, at least due to the deactivation of ERK1/2 and JNK1/2 MAP kinase signaling pathways. Moreover, RES-LA exhibited a strong effect protecting the TNF-α-induced exacerbated permeability in an HUVEC in vitro monolayer (by 81%) via the integrity protection of intercellular junction proteins from the MMP-9 activity. This effect was confirmed by using several complementary approaches including, the real-time monitoring of trans-endothelial electric resistance (TEER), the Transwell HUVEC permeability level, the microscopic examination of the platelet endothelial cell adhesion molecule-1 (CD31/PECAM-1) integrity as well as the fluorescence in intercellular spaces. Consequently, following this strong in vitro proof-of-concept, there is a need to test this promising RES–lipid derivative compound to control the pathological endothelial permeability in vivo.

scholarly journals Application of ultrasound-assisted deep eutectic solvent extraction combined with liquid-liquid extraction method to the extraction of three pesticide residues from fruit and vegetable samples

2020 ◽  
pp. 1-7
Author(s):  
Sue Chen ◽  
Qi An ◽  
Hong Sun ◽  
Mengqian Mao
Keyword(s):  
Solvent Extraction ◽  
Pesticide Residues ◽  
High Performance ◽  
Extraction Efficiency ◽  
Deep Eutectic Solvent ◽  
Liquid Extraction ◽  
Fruit And Vegetable ◽  
Vegetable Samples ◽  
Liquid Liquid Extraction ◽  
Ultrasound Assisted

Abstract A simple, inexpensive and sensitive method was developed for the simultaneous determination of three pesticide residues (carbendazim, thiophanate-methyl, and imidacloprid) in fruit and vegetable samples using high performance liquid chromatography (HPLC) based on a combined pretreatment of ultrasound-assisted deep eutectic solvent extraction (UA-DES-E) and liquid-liquid extraction (LLE). In this study, various types of deep eutectic solvents (DESs) were synthesized and the extraction efficiency was compared as extraction solvents. Results showed that glycerol-proline = 9:4 (GP-5) obtained the highest extraction efficiency among different types of DESs. Experiment conditions, including DES volume, extraction time and pH, were systematically optimized using single-factor experiment. Under the optimum conditions, the limits of detection (LODs) and quantification (LOQs) were in the ranges of 0.05–0.2 μg·mL−1 and 0.1–0.5 μg·mL−1, respectively. The relative recoveries of the three pesticides in the fruit and vegetable samples ranged from 85.7 to 113.0% at two spiked levels. Meanwhile, the method achieved excellent linearity with determination coefficients (r) greater than 0.999. Furthermore, the method was successfully applied to the analysis of the pesticides in real fruit and vegetable samples (apple, tomato, and grape).

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