scholarly journals Ultrasound-Assisted Deep Eutectic Solvent Extraction of Anthocyanins from Blueberry Wine Residues: Optimization, Identification, and HepG2 Antitumor Activity

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5456
Author(s):  
Hongkun Xue ◽  
Jiaqi Tan ◽  
Qian Li ◽  
Jintian Tang ◽  
Xu Cai
Keyword(s):  
Solvent Extraction ◽  
Antitumor Activity ◽  
Deep Eutectic Solvent ◽  
Extraction Process ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Brewing Process ◽  
Ultrasound Assisted ◽  
Optimum Extraction ◽  
Better Than

Blueberry wine residues produced during the wine-brewing process contain abundant anthocyanins and other bioactive compounds. To extract anthocyanins from blueberry wine residues more efficiently, a novel procedure of ultrasound-assisted deep eutectic solvent extraction (UADESE) was proposed in this work. The extraction process was optimized by response surface methodology coupled with genetic algorithm. The optimum extraction parameters to achieve the highest yield of anthocyanins (9.32 ± 0.08 mg/g) from blueberry wine residues by UADESE were obtained at water content of 29%, ultrasonic power of 380 W, extraction temperature of 55 °C, and extraction time of 40 min. The AB-8 macroporous resin combined with Sephadex LH-20 techniques was used to purify the crude extract (CE) obtained under optimum extraction conditions and analyze the anthocyanins composition by HPLC-ESI-MS/MS. The cyanidin-3-rutinoside with purity of 92.81% was obtained. The HepG2 antitumor activity of CE was better than that of the purified anthocyanins component. Moreover, CE could increase the intracellular reactive oxygen species levels and the apoptosis, and arrest HepG2 cells in the S phases. These findings provided an effective and feasible method for anthocyanins extraction, and reduced the environmental burden of this waste.

Optimization of Ultrasonic Extraction Process by Response Surface Methodology of Polysaccharides from Limonium bicolor Kunze (Bge.)

2011 ◽  
Vol 317-319 ◽  
pp. 321-324
Author(s):  
Peng Zhao ◽  
Ou Li ◽  
A Ping Liu
Keyword(s):  
Extraction Process ◽  
Ultrasonic Extraction ◽  
Raw Material ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Limonium Bicolor ◽  
Predicted Values ◽  
Optimum Extraction ◽  
Extraction Power

Polysaccharide from Limonium bicolor Kunze (Bge.)(LBPs) was extracted with the assistance of ultrasonic.Box-Behnken design(BBD) was employed to optimize extraction time; extraction temperature;ultrasonic extraction power and ratio of water to raw material to obtain a high LBPs yield.The optimum extraction conditions were as follows: extraction temperature 81.23°C, ultrasonic power 190.86W, extraction time 42.39 min and ratio of water to raw material 15.4ml/g.The yield of polysaccharide was 7.11% based on the above mentioned conditions. Close agreement between experimetal and predicted values was found.

Optimization of Ultrasonic Extraction Process by Response Surface Methodology of Polysaccharides from Tussilago farfara L.

2013 ◽  
Vol 771 ◽  
pp. 41-44
Author(s):  
Peng Zhao ◽  
Xiao Song
Keyword(s):  
Response Surface Methodology ◽  
Extraction Process ◽  
Ultrasonic Extraction ◽  
Raw Material ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Tussilago Farfara ◽  
Optimum Extraction ◽  
Extraction Power

Polysaccharides from Tussilago farfara L.(TFPs) was extracted with the assistance of ultrasonic.Box-Behnken design (BBD) was employed to optimize extraction time; extraction temperature;ultrasonic extraction power and ratio of water to raw material to obtain a high TFPs yield.The optimum extraction conditions were as follows: extraction temperature 65.1°C, ultrasonic power 380.5W, extraction time 28.7 min and ratio of water to raw material 23.6ml/g.The yield of polysaccharide was 2.14% based on the above mentioned conditions.

scholarly journals Optimization Ultrasound-Assisted Deep Eutectic Solvent Extraction of Anthocyanins from Raspberry Using Response Surface Methodology Coupled with Genetic Algorithm

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1409
Author(s):  
Hongkun Xue ◽  
Jiaqi Tan ◽  
Qian Li ◽  
Jintian Tang ◽  
Xu Cai
Keyword(s):  
Genetic Algorithm ◽  
Response Surface Methodology ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Solvent Extraction ◽  
Response Surface ◽  
High Performance ◽  
Deep Eutectic Solvent ◽  
Ultrasound Assisted ◽  
Optimum Extraction

Raspberries have been reported to contain abundant anthocyanins and other active compounds. To extract anthocyanins from raspberries more efficiently, a novel procedure of ultrasound-assisted deep eutectic solvent extraction (UADESE) was proposed in this paper. The extraction process was optimized by response surface methodology coupled with a genetic algorithm. The optimum extraction parameters to achieve the highest yield of anthocyanins 1.378 ± 0.009 mg/g from raspberry powder via UADESE were obtained at a water content of 29%, ultrasonic power of 210 W, extraction temperature of 51 °C and extraction time of 32 min. The AB-8 macroporous resin combined with the high-speed counter current chromatography (HSCCC) method were further used to isolate and purify the anthocyanins extracts obtained under optimum extraction conditions, and the structure of purified anthocyanins components were identified by UV-Visible spectrophotometer (UV-Vis), high-performance liquid chromatography (HPLC), high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS/MS), 1H nuclear magnetic resonance (NMR) and 13C-NMR spectra. The two anthocyanins (cyanidin-3-glucoside with a purity of 92.25% and cyanidin-3-rutinoside with a purity of 93.07%) identified were consistent with those present in raspberries. These findings provided an effective and feasible method for extraction, isolation and purification of anthocyanins from natural plant resources.

scholarly journals Organic Acids and Polyphenols Determination in Polish Wines by Ultrasound-Assisted Solvent Extraction of Porous Membrane-Packed Liquid Samples

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4376 ◽  
Author(s):  
Robles ◽  
Fabjanowicz ◽  
Płotka-Wasylka ◽  
Konieczka
Keyword(s):  
Solvent Extraction ◽  
Organic Acids ◽  
Extraction Process ◽  
Porous Membrane ◽  
New Technique ◽  
Extraction Temperature ◽  
Extraction Solvent ◽  
Liquid Samples ◽  
Ultrasound Assisted ◽  
A New Technique

In the near future, Poland is going to have more and more favorable conditions for viticulture. Organic acids and polyphenols are among the most commonly analyzed compounds due to their beneficial properties for human health and their importance in the winemaking process. In this work, a new technique involving ultrasound-assisted solvent extraction of porous membrane-packed liquid samples (UASE-PMLS) was for the first time described and applied for real samples. The methodology based on UASE-PMLS for organic acids and polyphenols in wine samples was optimized and validated. Using the new technique coupled to GC–MS, organic acids and polyphenols were evaluated in Polish wine samples. Extraction solvent, extraction temperature, derivatization time and sample pH were optimized. Chemometric tools were used for data treatment. Good linearity was obtained for the concentration ranges evaluated with r values between 0.9852 and 0.9993. All parameters of method validation (intra- and inter-day precision and matrix effect) were over 80% with coefficient of variation (CV) up to 17%. Recovery was between (92.0 ± 8.5)% and (113 ± 16)%. Finally, green assessment was evaluated using Analytical Eco-Scale and Green Analytical Procedure Index (GAPI). The UASE-PMLS is characterized by many advantages, e.g., the extraction process is fast and easy coupled to GC–MS. Regarding other extraction techniques, the amount of used solvent is minimum, and no waste is generated. Therefore, it is an environmentally friendly technique.

Ultrasound-assisted deep eutectic solvent extraction of echinacoside and oleuropein from Syringa pubescens Turcz.

2020 ◽  
Vol 151 ◽  
pp. 112442 ◽  
Author(s):  
Xinsheng Wang ◽  
Yanfang Wu ◽  
Jie Li ◽  
Aoxiang Wang ◽  
Guangyao Li ◽  
...  
Keyword(s):  
Solvent Extraction ◽  
Deep Eutectic Solvent ◽  
Ultrasound Assisted

ULTRASONIC-ASSISTED EXTRACTION OF OIL FROM CALOPHYLLUM INOPHYLLUM SEEDS: OPTIMIZATION OF PROCESS PARAMETERS

2016 ◽  
Vol 78 (10) ◽  
Author(s):  
Faiznur Mohd Fuad ◽  
Mashitah Mat Don
Keyword(s):  
Solvent Extraction ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Calophyllum Inophyllum ◽  
Power Extraction ◽  
Optimization Of Process Parameters ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Calophyllum inophyllum (C. inophyllum) is a plant known as “Penaga Laut” in Malaysia. Its seeds contain valuable oil that serve as a potential alternative sources for vegetable oil. In this study, C. inophyllum seeds oil was extracted using an ultrasonic-assisted extraction (UAE) technique. The optimization of extraction parameters namely different types of solvent, extraction time, ultrasonic power, extraction temperature and liquid to solid (L/S) ratio were performed using one-factor-at-a-time (OFAT) method. The optimum extraction conditions obtained were n-hexane as a solvent, extraction time 20 min, ultrasonic power 210 W, extraction temperature 40°C and L/S ratio 20 ml/g, with an oil yield 55.44 ± 0.53 %.

Ultrasonic Extraction Chuanxiong Polysaccharide of Influencing Factors

2012 ◽  
Vol 550-553 ◽  
pp. 1071-1075
Author(s):  
Yong Guang Bi ◽  
Chun Chun Liu
Keyword(s):  
Influencing Factors ◽  
Extraction Process ◽  
Extraction Rate ◽  
Ultrasonic Extraction ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Ultrasonic Time ◽  
Solid Liquid

In order to study the the Chuanxiong polysaccharide ultrasonic extraction process, examine the ultrasonic time, ultrasonic power, solid-liquid ratio and extraction temperature on the Chuanxiong polysaccharide extract rate. The results show that the role of time at 30min Polysaccharide better, the extraction rate is 26.63%. when the ultrasonic power is 320W, Polysaccharide extraction rate is 26.15%.When the solid-liquid ratio is 1:20, polysaccharide extraction rate is 27.73%. When the extraction temperature is 60°C, the polysaccharide extraction rate is 27.73%.

scholarly journals Utilization of Response Surface Methodology in Optimization of Polysaccharides Extraction from Vietnamese Red Ganoderma lucidum by Ultrasound-Assisted Enzymatic Method and Examination of Bioactivities of the Extract

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Dat Tran Do ◽  
Dang Hoang Lam ◽  
Tai Nguyen ◽  
Tran Thi Phuong Mai ◽  
Le Thao My Phan ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ganoderma Lucidum ◽  
Extraction Method ◽  
Gel Permeation Chromatography ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Anticancer Activities ◽  
Ultrasound Assisted

Red Ganoderma lucidum (G. lucidum) is a popular medicinal herb commonly used in Vietnamese traditional remedies due to its potential value for health. In this study, polysaccharides were extracted from G. lucidum using ultrasound-assisted enzymatic extraction method. The response surface methodology and Box–Behnken design were employed to investigate the effects of pH, extraction temperature, extraction time, and ultrasonic power on the content of polysaccharides. Based on ultraviolet-visible spectroscopy analysis, the highest content of polysaccharides in the extract was 32.08 mg/g under optimum experimental parameters including enzyme concentration of 3%, pH of 5.5, extraction temperature of 45°C, extraction time of 30 min, and ultrasonic power of 480 W. The Fourier-transform infrared spectroscopy was also used to identify the functional groups in the extracts. The molecular weights of polysaccharides were determined by gel permeation chromatography. The obtained extract was then evaluated for anticancer activities by using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, showing the anticancer activities with the half-maximal inhibitory concentration value of more than 512 μg/mL. This result suggested that UAEE could be considered as an appropriate and effective extraction method for bioactive crude polysaccharides from G. lucidum.

scholarly journals Optimizing Ultrasound-Assisted Deep Eutectic Solvent Extraction of Bioactive Compounds from Chinese Wild Rice

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2718 ◽  
Author(s):  
Jia Zeng ◽  
Yuqing Dou ◽  
Ning Yan ◽  
Na Li ◽  
Huaibao Zhang ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Wild Rice ◽  
Choline Chloride ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Deep Eutectic Solvent ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Green Solvents ◽  
Ultrasound Assisted

In this study, deep eutectic solvents (DESs) were used for the ultrasound-assisted extraction (UAE) of valuable bioactive compounds from Chinese wild rice (Zizania spp.). To this end, 7 different choline chloride (CC)-based DESs were tested as green extraction solvents. Choline chloride/1,4-butanediol (DES-2) exhibited the best extraction efficiency in terms of parameters such as the total flavonoid content (TFC), total phenolic content (TPC), and free radical scavenging capacity (DPPH● and ABTS●+). Subsequently, the UAE procedure using 76.6% DES-2 was also optimized: An extraction temperature of 51.2 °C and a solid–liquid ratio of 37.0 mg/mL were considered optimal by a Box–Behnken experiment. The optimized extraction procedure proved efficient for the extraction of 9 phenolic and 3 flavonoid compounds from Chinese wild rice as determined by quantification based on ultra-performance liquid chromatography–triple quadrupole tandem mass spectrometry (UPLC-QqQ-MS). This work, thus, demonstrates the possibility of customizing green solvents that offer greater extraction capacity than that of organic solvents.

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