Bovine serum albumin contained in culture medium used in artificial insemination is an important anaphylaxis risk factor

2008 ◽  
Vol 90 (5) ◽  
pp. 2013.e17-2013.e19 ◽  
Author(s):  
Juan A. Pagán ◽  
Idoia Postigo ◽  
Jorge R. Rodríguez-Pacheco ◽  
Maribel Peña ◽  
Jorge A. Guisantes ◽  
...  
Keyword(s):  
Risk Factor ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Artificial Insemination ◽  
Bovine Serum ◽  
Culture Medium

A Method of Obtaining a Suspension of Intact Parenchymal Cells from Adult Rat Liver

1971 ◽  
Vol 8 (1) ◽  
pp. 73-86
Author(s):  
JENNIFER J. GALLAI-HATCHARD ◽  
G. M. GRAY
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Culture Medium ◽  
High Yield ◽  
Alkaline Ph ◽  
High Osmolarity ◽  
Adult Rat ◽  
Parenchymal Cells ◽  
Phosphate Buffered Saline

The perfusion of liver with either citrate or tetraphenyl boron to remove Ca2+ or K+ or with a solution of high osmolarity and alkaline pH yields plenty of cells but they are all damaged. Perfusion of the liver with hyaluronidase and collagenase followed by incubation of liver slices in the same enzyme solution produced a high yield of cells (25%, w/w, of liver) of which only about 1% were undamaged. However, perfusion with 0.3% hyaluronidase, 0.3% collagenase and 0.1% trypsin in phosphate-buffered saline (excluding Mg2+ and Ca2+) followed by incubation at 25 °C of the chopped liver gave a small yield (2-4%, w/w) of undamaged cells which were not permeable to eosin for up to an hour when suspended in culture medium containing 2% bovine serum albumin.

scholarly journals PS48 can replace bovine serum albumin in pig embryo culture medium, and improve in vitro embryo development by phosphorylating AKT

2015 ◽  
Vol 82 (4) ◽  
pp. 315-320 ◽  
Author(s):  
Lee D. Spate ◽  
Alana Brown ◽  
Bethany K. Redel ◽  
Kristin M. Whitworth ◽  
Randall S. Prather
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Embryo Development ◽  
Embryo Culture ◽  
Bovine Serum ◽  
Culture Medium ◽  
Embryo Culture Medium

scholarly journals Optimization of nano-encapsulation on neonatal porcine islet-like cell clusters using polymersomes

2020 ◽  
Author(s):  
Sang Hoon Lee ◽  
Hyun-Ouk Kim ◽  
Jung-Taek Kang
Keyword(s):  
Extracellular Matrix ◽  
Polyethylene Glycol ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Culture Medium ◽  
Cell Clusters ◽  
Porcine Islet ◽  
Minimal Damage ◽  
Amine Groups

Abstract ObjectiveResearches proving methods for nano encapsulation of neonatal porcine islet-like cell clusters (NPCCs) using polymersomes (PSomes) formed using polymers of polyethylene glycol-block-poly lactide (PEG-b-PLA). Herein, our studies present efficient nano encapsulation procedure with minimal damage and loss of NPCCs. MethodsWe used N-hydroxysuccinimide (NHS) on the N-terminal of PSomes to induce binding of amine groups in the extracellular matrix surrounding NPCCs. F-10 culture medium with bovine serum albumin was used in the nano-encapsulation procedure to minimize damage and loss of NPCCs. Finally, we induced crosslinking between bi-functional PSomes (NHS-/NH2-PSomes). ResultsF-10 culture medium containing 0.25% BSA with pH of 7.3 minimized the damage and loss of NPCCs after nano-encapsulation as compared with using basic HBSS buffer (pH 8.0). Also, we induced the efficiency nano encapsulation through conjugation of PSomes using bi-functional PSomes (NHS-/NH2-PSomes).

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

1981 ◽  
Vol 46 (03) ◽  
pp. 645-647 ◽  
Author(s):  
M A Orchard ◽  
C Robinson
Keyword(s):  
Platelet Aggregation ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protective Effect ◽  
Whole Blood ◽  
Bovine Serum ◽  
Fatty Acid Content ◽  
Krebs Solution ◽  
Antiaggregatory Activity ◽  
Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

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