Sequential treatment of hydrogen peroxide, vacuum packaging, and dry heat for inactivating Salmonella Typhimurium on alfalfa seeds without detrimental effect on seeds viability

2019 ◽  
Vol 77 ◽  
pp. 130-136 ◽  
Author(s):  
Eun-Jeong Hong ◽  
Sang-Hyun Park ◽  
Dong-Hyun Kang
Keyword(s):  
Hydrogen Peroxide ◽  
Salmonella Typhimurium ◽  
Detrimental Effect ◽  
Vacuum Packaging ◽  
Sequential Treatment ◽  
Dry Heat ◽  
Alfalfa Seeds

scholarly journals The use of germicidal ultraviolet light, vaporised hydrogen peroxide and dry heat to decontaminate face masks and filtering respirators contaminated with an infectious norovirus

2020 ◽  
pp. 100111
Author(s):  
Constance Wielick ◽  
Louisa F. Ludwig-Begall ◽  
Lorène Dams ◽  
Ravo M. Razafimahefa ◽  
Pierre-Francois Demeuldre ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Ultraviolet Light ◽  
Dry Heat

Growth of Salmonella typhimurium and Mesophilic Organisms on Beef Steaks as Influenced by Type of Packaging1,2

1982 ◽  
Vol 45 (3) ◽  
pp. 263-267 ◽  
Author(s):  
L. S. LUITEN ◽  
J. A. MARCHELLO ◽  
F. D. DRYDEN
Keyword(s):  
Treatment Group ◽  
Salmonella Typhimurium ◽  
Vacuum Packaging ◽  
Gas Atmosphere ◽  
Beef Steaks ◽  
Retail Meat

Two trials, each utilizing 72 samples of fresh beef loin steak, were done to determine the effects of various packing systems upon growth of Salmonella typhimurium. Samples were inoculated with 105 cells/cm2 of the organism and randomly assigned to four packaging treatments: (1) overwrapping in oxygen-permeable film; (2) vacuum packaging; (3) packaging in barrier bags flushed with a 60% CO2: 40% O2 gas atmosphere then evacuated and sealed; and (4) packaging in barrier bags filled with a 60% CO2: 40% O2 gas atmosphere. Twelve steak samples were inoculated with S. typhimurium and 6 were uninoculated and served as a control in each treatment group. Samples were displayed in retail meat cases at 10 C for 3, 6 or 9 days, when they were evaluated for shrinkage and numbers of mesophilic organisms and S. typhimurium. Percent shrinkage was not affected (P>0.05) by packaging treatment. Counts of mesophilic organisms were similar (P>0.05) for vacuum- and gas-treated steaks, which were significantly lower (P<0.05) than counts from film overwrapped samples. Numbers of S. typhimurium increased significantly (P<0.05) during storage on samples wrapped with oxygen permeable film but remained low and fairly constant for vacuum- or gas-treated steaks. After 9 days of display, the film overwrapped steaks had greater (P<0.05) numbers of S. typhimurium than those of other treatments, whereas steaks held within the 60% CO2: 40% O2 gas atmosphere had lowest numbers overall.

Antimicrobial Effects of Novel H2O2-Ag+ Complex on Membrane Damage to Staphylococcus aureus,Escherichia coli and Salmonella typhimurium

2021 ◽  
Author(s):  
Bing Han ◽  
Xiaoyu Han ◽  
Mengmeng Ren ◽  
Yilin You ◽  
Jicheng Zhan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Salmonella Typhimurium ◽  
Membrane Damage ◽  
Bactericidal Effect ◽  
E Coli ◽  
Bacterial Cell Membrane ◽  
Time Kill ◽  
Environmental Disinfection

Diseases caused by harmful microorganisms pose a serious threat to human health. Safe and environment-friendly disinfectants are, therefore, essential in preventing and controlling such pathogens. This study aimed to investigate the antimicrobial activity and mechanism of a novel hydrogen peroxide and silver (H 2 O 2 -Ag + ) complex (HSC) in combatting Staphylococcus aureus ATCC 29213, Escherichia coli O157:H7 NCTC 12900 and Salmonella typhimurium SL 1344. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values against S. aureus were found to be 0.014 % H 2 O 2 -3.125 mg/L Ag + , while 0.028 % H 2 O 2 -6.25 mg/L Ag + for both E. coli and S. typhimurium . Results of the growth curve assay and time-kill trial suggest that the HSC could inhibit the growth of the tested bacteria, as 99.9 % of viable cells were killed following treatment at the 1 MIC for 3 h. Compared with Oxytech D10 disinfectant (0.25 % H 2 O 2 -5 mg/L Ag + ), the HSC exhibited better antibacterial efficacy at a lower concentration (0.045 % H 2 O 2 -10 mg/L Ag + ). The mechanism of antibacterial action of HSC was found including the disruption of the bacterial cell membrane, followed by entry into the bacteria cell to reduce intracellular adenosine triphosphate (ATP) concentration, and inhibit the activity of antioxidases, superoxide dismutase (SOD) and catalase (CAT). The enhanced bactericidal effect of hydrogen peroxide combined with silver indicates a potential for its application in environmental disinfection, particularly in the food industry.

scholarly journals The use of germicidal ultraviolet light, vaporised hydrogen peroxide and dry heat to decontaminate face masks and filtering respirators contaminated with an infectious norovirus

2020 ◽  
Author(s):  
Constance Wielick ◽  
Louisa Fiona Ludwig-Begall ◽  
Lorene Dams ◽  
Ravo Michele Razafimahefa ◽  
Pierre-Francois Demeuldre ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Ultraviolet Light ◽  
Murine Norovirus ◽  
Ultraviolet Germicidal Irradiation ◽  
Dry Heat ◽  
Surgical Masks ◽  
Oral Pathogens

In the context of the SARS-CoV-2 pandemic, reuse of surgical masks and filtering facepiece respirators has been recommended. Their reuse necessitates procedures to inactivate contaminating human respiratory and oral pathogens. We previously demonstrated decontamination of masks and respirators contaminated with an infectious SARS-CoV-2 surrogate via ultraviolet germicidal irradiation, vaporised hydrogen peroxide, and use of dry heat. Here, we show that these same methods efficiently inactivate a more resistant, non-enveloped oral virus; decontamination of infectious murine norovirus-contaminated masks and respirators reduced viral titres by over four orders of magnitude on mask or respirator coupons.

Decontamination Strategies for Filtering Facepiece Respirators (FFRs) in Healthcare Organizations: A Comprehensive Review

2020 ◽  
Author(s):  
Ashok Kumar Jena ◽  
Jitendra Sharan
Keyword(s):  
Hydrogen Peroxide ◽  
Ethylene Oxide ◽  
Time Use ◽  
Ultraviolet Irradiation ◽  
The Other ◽  
Limited Time ◽  
Healthcare Organizations ◽  
Comprehensive Review ◽  
Dry Heat ◽  
Other Hand

Abstract Filtering facepiece respirators (FFRs) are made for one-time use. A massive shortage of FFRs is widespread during pandemic events and has forced many healthcare organizations to decontaminate them and re-use for a limited time. Many decontamination methods have been proposed for the decontamination of FFRs. This review highlights various aspects of decontamination methods available in the literature. Among various methods available, vaporized hydrogen peroxide, ultraviolet irradiation, and dry heat seem to be the most promising decontaminants for FFRs. On the other hand, microwave, bleach, ethylene oxide, alcohol, hydrogen peroxide liquid, sanitizing wipes, and soap and water are not recommended methods for FFR decontamination.

Chemical and Irradiation Treatments for Killing Escherichia coli O157:H7 on Alfalfa, Radish, and Mung Bean Seeds

2003 ◽  
Vol 66 (5) ◽  
pp. 767-774 ◽  
Author(s):  
M. L. BARI ◽  
E. NAZUKA ◽  
Y. SABINA ◽  
S. TODORIKI ◽  
K. ISSHIKI
Keyword(s):  
Escherichia Coli ◽  
Heat Treatment ◽  
Mung Bean ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Chemical Treatments ◽  
Dry Heat ◽  
Pathogen Populations ◽  
Dry Heat Treatment ◽  
Alfalfa Seeds

In this study, the effectiveness of dry-heat treatment in combination with chemical treatments (electrolyzed oxidizing [EO] water, califresh-S, 200 ppm of active chlorinated water) with and without sonication in eliminating Escherichia coli O157:H7 on laboratory-inoculated alfalfa, radish, and mung bean seeds was compared with that of dry-heat treatment in combination with irradiation treatment. The treatment of mung bean seeds with EO water in combination with sonication followed by a rinse with sterile distilled water resulted in reductions of approximately 4.0 log10 CFU of E. coli O157:H7 per g, whereas reductions of ca. 1.52 and 2.64 log10 CFU/g were obtained for radish and alfalfa seeds. The maximum reduction (3.70 log10 CFU/g) for mung bean seeds was achieved by treatment with califresh-S and chlorinated water (200 ppm) in combination with sonication and a rinse. The combination of dry heat, hot EO water treatment, and sonication was able to eliminate pathogen populations on mung bean seeds but was unable to eliminate the pathogen on radish and alfalfa seeds. Other chemical treatments used were effective in greatly reducing pathogen populations on radish and alfalfa seeds without compromising the quality of the sprouts, but these treatments did not result in the elimination of pathogens from radish and alfalfa seeds. Moreover, a combination of dry-heat and irradiation treatments was effective in eliminating E. coli O157:H7 on laboratory-inoculated alfalfa, radish, and mung bean seeds. An irradiation dose of 2.0 kGy in combination with dry heat eliminated E. coli O157:H7 completely from alfalfa and mung bean seeds, whereas a 2.5-kGy dose of irradiation was required to eliminate the pathogen completely from radish seeds. Dry heat in combination with irradiation doses of up to 2.0 kGy did not unacceptably decrease the germination percentage for alfalfa seeds or the length of alfalfa sprouts but did decrease the lengths of radish and mung bean sprouts.

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