Bioactivities of crude caffeine: Antioxidant activity, cyclooxygenase-2 inhibition, and enhanced glucose uptake

Abstract The study assessed the health-promoting properties and the content of minerals in the bark of bird cherry (Prunus padus L.), which was then used as an ingredient in functional teas. The infusions were made with the use of Matricaria chamomilla L., Tilia cordata Mill., and Calendula officinalis L., and then combined with the bark in various proportions. The prepared infusions were tested for antioxidant activity, ability to reduce copper ions and iron ions, as well as the ability to scavenge hydroxyl radicals. In the next stage, the antimicrobial activity and the ability to inhibit the enzyme cycloxygenase-2 were assessed. Bird cherry bark contains a high potassium content of 19.457 ± 762 mg/kg d.m. In all the tests evaluating the antioxidant activity, infusions from the bark of bird cherry alone and with its 30% addition had the strongest properties. The analyzed infusions also have the ability to reduce Cu(ii) ions; they are active to reduce Fe(iii) ions and scavenge hydroxyl radical. The highest antimicrobial activity was found for teas with 20 and 30% bark, especially against Listeria monocytogenes (25.0–27.0 mm) (±3.0). The bark infusion was also found to have the highest inhibitory activity against cyclooxygenase-2 (COX-2) – 77.0%.

Download Full-text

In-vitro Investigation of Polyphenol-Rich Date (Phoenix dactylifera L.) Seed Extract Bioactivity

Frontiers in Nutrition ◽

10.3389/fnut.2021.667514 ◽

2021 ◽

Vol 8 ◽

Author(s):

Serene Hilary ◽

Jaleel Kizhakkayil ◽

Usama Souka ◽

Fatima Al-Meqbaali ◽

Wissam Ibrahim ◽

...

Keyword(s):

Antioxidant Activity ◽

Glucose Uptake ◽

Cell Lines ◽

Lipid Accumulation ◽

Seed Extract ◽

C2c12 Cells ◽

Hep G2 ◽

Date Seed ◽

Mcf 7

Date seeds are a by-product of the date fruit processing industry with minimal human use; however, they are a rich source of polyphenols with a range of potential biological properties. The current study investigates the cytotoxicity of date seed polyphenols against cancer cell lines, its ability to combat hyperglycemia, its antioxidant potential and its anti-adipogenic effect. The present work aimed to establish the usefulness of date seeds in the food industry as a functional ingredient. The anti-tumour activity of DSE was tested in a panel of cell lines such as MCF-7, MDA-MB-231, Hep-G2, Caco-2, and PC-3 by measuring cell viability and cleaved PARP. Lipid accumulation and effect on the differentiation of 3T3-L1 cells (adipocytes) were tested with date seed extract treatments. The influence of date seed polyphenols on glucose uptake was studied in 3T3-L1 cells and C2C12 cells (muscle cells). The antioxidant activity of the polyphenols from date seed products such as date seed extract (DSE), date seed powder (DSP), and date seeds fortified bread (DSB) was tested following in-vitro digestion to study their stability in the gastrointestinal milieu. DSE treatment resulted in significantly reduced viability in MCF-7 and Hep-G2 cells with 48-h treatments. Glucose uptake increased in the adipocytes with DSE treatments; moreover, it inhibited adipocyte differentiation and lipid accumulation. DSE decreased the expression levels of PPAR-γ, C/EBPα, adiponectin and upregulated GLUT-4, and phospho-AMPK. This study also found that date seed samples retained antioxidant activity in the digestive milieu and concludes that the date seed polyphenols remain active in the digestive milieu and exhibit potential anti-hyperglycemic and anti-adipogenic activity.

Download Full-text

Expression of Cyclooxygenase-2, Nitric Oxide Synthase 2 and Heme Oxygenase-1 mRNA Induced by Bis-Eugenol in RAW264.7 Cells and their Antioxidant Activity Determined Using the Induction Period Method

In Vivo ◽

10.21873/invivo.11135 ◽

2018 ◽

Vol 31 (5) ◽

Keyword(s):

Nitric Oxide ◽

Antioxidant Activity ◽

Nitric Oxide Synthase ◽

Induction Period ◽

Heme Oxygenase ◽

Cyclooxygenase 2 ◽

Raw264.7 Cells ◽

Heme Oxygenase 1 ◽

Nitric Oxide Synthase 2 ◽

Oxide Synthase

Download Full-text

In vitro antioxidant activity, cyclooxygenase-2, thioredoxin reductase inhibition and DNA protection properties of Teucrium sandrasicum L.

Industrial Crops and Products ◽

10.1016/j.indcrop.2015.05.025 ◽

2015 ◽

Vol 74 ◽

pp. 545-550 ◽

Cited By ~ 8

Author(s):

Pinar Aksoy-Sagirli ◽

Nurten Ozsoy ◽

Gulay Ecevit-Genc ◽

Gulay Melikoglu

Keyword(s):

Antioxidant Activity ◽

Thioredoxin Reductase ◽

Cyclooxygenase 2 ◽

Dna Protection ◽

In Vitro Antioxidant Activity

Download Full-text

IN VITRO ANTIDIABETIC ACTIVITY BY GLUCOSE UPTAKE OF YEAST CELL ASSAY AND ANTIOXIDANT POTENTIAL OF ANNONA RETICULATA L. LEAF EXTRACTS

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2020.120301 ◽

2020 ◽

pp. 208-213

Author(s):

Vineela Pulivarthi ◽

Penchalaneni Josthna ◽

Challagundla Varadarajulu Naidu

Keyword(s):

Antioxidant Activity ◽

Yeast Cell ◽

Glucose Uptake ◽

Cell Model ◽

Reducing Power ◽

Antioxidant Potential ◽

Antidiabetic Activity ◽

Leaf Extracts ◽

Reducing Power Assay

The undesirable adverse effects of present available synthetic drugs endorse the modern medicine to search the superior choice for the treatment of metabolic diseases. Herbal medicine turns out to be a hopeful therapy for the effective treatment of diabetes in foreseeable future. In an effort to render a scientific evidence for the antidiabetic potential of Annona reticulata L., the present research, with the objective to evaluate the ability of A. reticulata leaf extracts on antihyperglycemic property under in vitro using yeast cell model was performed. Besides, as the diabetes and its complications are highly associated with the oxidative stress, the current study was also focussed on antioxidant property of A. reticulata leaf extracts. The scavenging ability of plant extracts on free radical 2, 2 – diphenyl- 1- picrylhydrazyl (DPPH), ferric reducing power assay and total antioxidant activity were performed to establish the antioxidant potential of A. reticulata leaf extracts. The in vitro antidiabetic ability of A. reticulata leaves was evaluated by glucose uptake method using yeast cell model. Among the four chosen solvent extracts- aqueous, methanol, ethyl acetate and n-hexane, methanol extract (MeE) exhibited high antioxidant potential. MeE exhibited 62.28% of DPPH inhibition at 200µg/ml, with total antioxidant activity 164.72 ± 2.63µg/ml and higher absorbance in reducing power assay (1.15 ± 0.03). In vitro antidiabetic activity by glucose uptake of yeast cell assay was evaluated and observed dose dependant rise in % of glucose uptake in methanol, ethylacetate and aqueous extracts of A. reticulata. MeE showed 48.55% of glucose uptake at 500µg/ml concentration. Hence the present study could be concluded as A.reticulata leaves possess potent antioxidant activity and antidiabetic activity under in vitro conditions. With the outcome of the present initial study, research work could be extended further; thereby the exact pharmacological action of the plant compounds could be discovered.

Download Full-text

Phytochemical profile, antimicrobial, antioxidant activity and Cyclooxygenase 2 inhibitory properties of nutmeg (Myristica fragrans) seeds extract

Egyptian Journal of Chemistry ◽

10.21608/ejchem.2021.78192.3831 ◽

2021 ◽

Vol 0 (0) ◽

pp. 0-0

Author(s):

Jwan Abdulsattar ◽

Muthanna Orabi ◽

Zaizafoon Nasi

Keyword(s):

Antioxidant Activity ◽

Cyclooxygenase 2 ◽

Myristica Fragrans ◽

Phytochemical Profile

Download Full-text

In Vitro Anti-diabetic Activity of Sclerocarya Birrea and Ziziphus Mucronata

Natural Product Communications ◽

10.1177/1934578x1300800924 ◽

2013 ◽

Vol 8 (9) ◽

pp. 1934578X1300800 ◽

Cited By ~ 1

Author(s):

Nuno M.H. Da Costa Mousinho ◽

Jacob J. van Tonder ◽

Vanessa Steenkamp

Keyword(s):

Antioxidant Activity ◽

Insulin Secretion ◽

Side Effects ◽

Glucose Uptake ◽

C2c12 Myotubes ◽

Dependent Manner ◽

Methanol Extracts ◽

Sclerocarya Birrea ◽

Ziziphus Mucronata

Type 2 diabetes mellitus is on the increase worldwide. Current treatments possess undesirable side-effects and therefore investigations into alternative remedies, which may be cost-effective and devoid of such side-effects, are on-going. Aqueous and methanol extracts of the bark of Sclerocarya birrea (A. Rich.) Hochst. subsp. caffra (Sond.) Kokwaro (Anacardiaceae) and Ziziphus mucronata Willd. subsp. mucronata (Rhamnaceae) were subjected to testing in order to assess their in vitro anti-diabetic activity. Total polyphenolic content and antioxidant activity, as well as the effect on α-amylase, α-glucosidase and cytotoxicity, were measured. Furthermore, glucose uptake was evaluated in C2C12 myotubes, 3T3-L1 adipocytes and HepG2 hepatocarcinoma cells, and insulin secretion in RIN-m5F rat pancreatic β-cells. The extracts of both plants inhibited the activities of α-amylase and α-glucosidase in a concentration dependent manner, with results being comparable with the positive control, acarbose. All the extracts contained antioxidant activity, with the methanol extracts of S. birrea displaying the strongest free radical scavenging capacity (IC50 = 2.16 μg/mL). Most of the extracts were not cytotoxic to the cell lines with IC50 values > 100 μg/mL. Extracts of both plants significantly increased glucose uptake in C2C12, 3T3-L1 and HepG2 cells. However, insulin secretion from RIN-m5F cells was not altered. This study provides evidence that the plants possess in vitro anti-diabetic properties.

Download Full-text