High-throughput histamine analysis approach in an official control laboratory: Analytical methods and four years fish products results

2014 ◽  
Vol 153 ◽  
pp. 437-443 ◽  
Author(s):  
Arianna Piersanti ◽  
Tamara Tavoloni ◽  
Carmela Lestingi ◽  
Roberta Galarini
Keyword(s):  
High Throughput ◽  
Analytical Methods ◽  
Analysis Approach ◽  
Control Laboratory ◽  
Fish Products ◽  
Official Control

An overview of analytical methods for monitoring bacterial transglycosylation

2014 ◽  
Vol 6 (19) ◽  
pp. 7590-7596 ◽  
Author(s):  
Bart Blanchaert ◽  
Erwin Adams ◽  
Ann Van Schepdael
Keyword(s):  
High Throughput ◽  
Analytical Methods ◽  
High Throughput Screens

This review highlights the fluorescence and radioactively labeled assays and high-throughput screens for the search for antibiotics targeting bacterial transglycosylation.

High resolution and high throughput analytical methods for d-tagatose and process related impurities using capillary electrophoresis

2020 ◽  
Vol 609 ◽  
pp. 113981
Author(s):  
Sri Rama Krishna Surapureddi ◽  
Kunta Ravindhranath ◽  
Ghantasala S. Sameer Kumar ◽  
Prashanth Chiliveri ◽  
Sreedhar Reddy Sappidi
Keyword(s):  
Capillary Electrophoresis ◽  
High Resolution ◽  
High Throughput ◽  
Analytical Methods ◽  
Related Impurities

European official control of food: Determination of histamine in fish products by a HPLC–UV-DAD method

2016 ◽  
Vol 211 ◽  
pp. 694-699 ◽  
Author(s):  
I. Altieri ◽  
A. Semeraro ◽  
F. Scalise ◽  
I. Calderari ◽  
P. Stacchini
Keyword(s):  
Fish Products ◽  
Official Control

scholarly journals From the Laboratory to The Vineyard—Evolution of The Measurement of Grape Composition using NIR Spectroscopy towards High-Throughput Analysis

2019 ◽  
Vol 8 (4) ◽  
pp. 21
Author(s):  
Aoife Power ◽  
Vi Khanh Truong ◽  
James Chapman ◽  
Daniel Cozzolino
Keyword(s):  
Chemical Composition ◽  
Hyperspectral Imaging ◽  
High Throughput ◽  
Analytical Methods ◽  
Near Infrared ◽  
Nir Spectroscopy ◽  
Chemical Compounds ◽  
Spectroscopic Techniques ◽  
High Throughput Analysis ◽  
Laboratory Methods

Compared to traditional laboratory methods, spectroscopic techniques (e.g., near infrared, hyperspectral imaging) provide analysts with an innovative and improved understanding of complex issues by determining several chemical compounds and metabolites at once, allowing for the collection of the sample “fingerprint”. These techniques have the potential to deliver high-throughput options for the analysis of the chemical composition of grapes in the laboratory, the vineyard and before or during harvest, to provide better insights of the chemistry, nutrition and physiology of grapes. Faster computers, the development of software and portable easy to use spectrophotometers and data analytical methods allow for the development of innovative applications of these techniques for the analyses of grape composition.

scholarly journals A 5-Years (2015–2019) Control Activity of an EU Laboratory: Contamination of Histamine in Fish Products and Exposure Assessment

2020 ◽  
Vol 10 (23) ◽  
pp. 8693
Author(s):  
Sonia Lo Magro ◽  
Simona Summa ◽  
Marco Iammarino ◽  
Pasquale D’Antini ◽  
Giuliana Marchesani ◽  
...  
Keyword(s):  
High Throughput ◽  
Elisa Test ◽  
Risk Exposure ◽  
Histamine Concentration ◽  
Control Activity ◽  
Fish Products ◽  
Canned Tuna ◽  
Two Samples ◽  
And Storage ◽  
Laboratory Contamination

Histamine contamination was evaluated on 474 batches (3130 determinations) of fish products collected in Puglia and Basilicata (southern part of Italy) during the years 2015–2019, using a high-throughput two-tier approach involving a screening (ELISA test) and confirmatory method (HPLC/FLD with o-phthalaldehyde derivatization). Histamine concentration >2.5 mg kg−1 was detected in 51% of total batches with the 2.5% of non-compliance. Except for two samples of fresh anchovies, all non-compliant samples were frozen, defrosted and canned tuna. Among 111 fresh tuna batches, 9 had a content of histamine between 393 and 5542 mg kg−1, and scombroid poisoning cases were observed after their consumption. Good quality canned tuna and ripened anchovies sold in Italy was observed. Furthermore, the analysis of the processing technology and storage practice critical points were reported in this study, with useful considerations to minimize the histamine risk for consumers. Finally, based on these results, several considerations about risk exposure were reported.

Development and Application of Activity-based Fluorescent Probes for High-Throughput Screening

2021 ◽  
Vol 28 ◽  
Author(s):  
Juan Cheng ◽  
Xin Li
Keyword(s):  
Drug Development ◽  
High Throughput ◽  
Fluorescent Probes ◽  
Analytical Methods ◽  
High Throughput Screening ◽  
High Sensitivity ◽  
Rapid Identification ◽  
Screening Assays ◽  
Hit Identification ◽  
Working Mechanisms

: High-throughput screening facilitates the rapid identification of novel hit compounds; however, it remains challenging to design effective high-throughput assays, partially due to the difficulty of achieving sensitivity in the assay techniques. Among the various analytical methods that are used, fluorescence-based assays dominate owing to their high sensitivity and ease of operation. Recent advances in activity-based sensing/imaging have further expanded the availability of fluorescent probes as monitors for high-throughput screening of result outputs. In this study, we have reviewed various activity-based fluorescent probes used in high-throughput screening assays, emphasizing their structure-related working mechanisms. Moreover, we have explored the possibility of the development of additional and better probes to boost hit identification and drug development against various targets.

Enzymatic-fluorometric analyses for glutamine, glutamate and free amino groups in protein-free plasma and milk

2017 ◽  
Vol 84 (1) ◽  
pp. 32-35 ◽  
Author(s):  
Torben Larsen ◽  
Carlos Fernández
Keyword(s):  
Glutamic Acid ◽  
Blood Plasma ◽  
High Throughput ◽  
Analytical Methods ◽  
Free Amino ◽  
Amino Groups ◽  
Technical Research ◽  
Animal Trials ◽  
Working Day ◽  
Free Plasma

This Technical Research Communication describes new analytical methods for free, unbound glutamic acid and glutamine in protein-free blood plasma and milk and introduces the use of quantitation of free amino groups in the same matrices for descriptive and analytical purposes. The present enzymatic-fluorometric methods are easily performed within one working day, allowing for ‘high throughput’ assays of animal trials. These assays could support and enable further studies in lactation physiology with the objective of improved metabolic health.

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