scholarly journals Comparison of gravimetric, creamatocrit and esterified fatty acid methods for determination of total fat content in human milk

2017 ◽  
Vol 217 ◽  
pp. 505-510 ◽  
Author(s):  
Jian Du ◽  
Melvin C.L. Gay ◽  
Ching Tat Lai ◽  
Robert D. Trengove ◽  
Peter E. Hartmann ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Human Milk ◽  
Fat Content ◽  
Total Fat

scholarly journals Determination of Fat Content and Fatty Acid Composition in Meat and Meat Products after Supercritical Fluid Extraction

2002 ◽  
Vol 85 (5) ◽  
pp. 1064-1069 ◽  
Author(s):  
Hans Berg ◽  
LenaDahlberg Dahlberg ◽  
Lennart Mathiasson
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Supercritical Fluid ◽  
Acid Composition ◽  
Meat Products ◽  
Relative Difference ◽  
Fat Content ◽  
Conventional Solvent Extraction ◽  
Total Fat

Abstract Two different relatively simple, commercially available supercritical fluid extractors (SFE), Leco and Foss-Tecator, were tested for the determination of total fat content in meat and meat products. The fatty acid composition in meat and meat products was also determined after the Foss-Tecator extraction in an aliquot of the extract. Total fat was determined by weighing after the different extraction procedures and the fatty acid composition by gas chromatography after hydrolysis and methylation of the extract. The results for total fat content agreed well with results from a standard method of Schmid, Bondzynski, and Ratzlaff, which uses conventional solvent extraction. Fatty acid composition was compared with the Bligh and Dyer extraction, and showed good agreement. The average relative difference between SFE and Bligh and Dyer of all fatty acids in the sample was <3% for acids exceeding 0.5% of total fatty acid amount. The advantages of SFE over traditional methods are a much lower consumption of hazardous organic solvents and shorter extraction times. To obtain quantitative recoveries by SFE, ethanol was added to the extraction cells before extraction.

Development of a new SFE method for rapid determination of total fat content of food

1993 ◽  
Vol 35 (9-12) ◽  
pp. 509-516 ◽  
Author(s):  
P. Lembke ◽  
H. Engelhardt
Keyword(s):  
Rapid Determination ◽  
Fat Content ◽  
Total Fat

scholarly journals PENENTUAN KANDUNGAN LEMAK DAN KOMPOSISI ASAM-ASAM LEMAK PADA BAGIAN BADAN IKAN GABUS (Channa striata) BUDIDAYA DAN LIAR

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Alvy M. Umage ◽  
Julius Pontoh ◽  
Lidya I. Momuat
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Extraction Method ◽  
Fat Content ◽  
The Body ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Channa Striata

ABSTRAK Telah dilakukan penentuan kandungan lemak dan komposisi asam-asam lemak pada bagian badan ikan gabus (Channa striata) budidaya dan liar. Penelitian ini bertujuan untuk mengetahui berapa kandungan lemak dan komposisi asam lemak pada bagian badan ikan gabus (Channa striata) budidaya dan liar. Kandungan lemak dilakukan menggunakan metode ektraksi dan diperoleh kandungan lemak bagian badan ikan gabus budidaya 0,07% dan ikan gabus liar 0,01%. Lemak ikan diderivatisasi dan dianalisis menggunakan kromatografi gas dan diperoleh komposisi asam lemak tertinggi pada bagian badan ikan gabus budidaya adalah asam lemak oleat 37,1% diikuti  dan diikuti oleh asam lemak palmitat 25,6% sedangkan asam lemak tertinggi pada bagian badan ikan gabus liar adalah asam lemak palmitat 28,6% dan diikuti asam lemak oleat 16,9%. Kandungan asam lemak omega-3 pada bagian badan ikan gabus budidaya 5,6% dan ikan gabus liar 9,0%. Kata kunci: Asam lemak, ikan gabus budidaya dan liar, kromatografi gas  ABSTRACT Determination of fat content and composition of fatty acids in the body of snakehead (Channa striata) from cultivated and wild sources. This study aims to determine how much fat content and composition fatty acids in the body of snakehead (Channa striata) from cultivated and wild sources. The fat content were carried out using the extraction method. The fat content of cultured snakehead body was 0.07% and that of wild fish was 0.01%. Fish fats were derivatized and analyzed using gas chromatography and the highest fatty acid composition on the body of cultivated snakehead fish is 37.1% oleic fatty acids followed by 25.6% palmitic fatty acids while the highest fatty acid in the body of a wild snakehead fish is 28.6% palmitic fatty acid and followed by 16.9% oleic fatty acid. The content of omega-3 fatty acids in the body of cultivated snakehead fish is 5.6% and 9.0% on wild snakehead fish. Keywords: Fatty acids, wild and cultivated snakehead fish, gas chromatography 

scholarly journals Polyunsaturated fatty acid concentrations in human hindmilk are stable throughout 12-months of lactation and provide a sustained intake to the infant during exclusive breastfeeding: an Italian study

2000 ◽  
Vol 84 (1) ◽  
pp. 103-109 ◽  
Author(s):  
Franca Marangoni ◽  
Carlo Agostoni ◽  
Anna M. Lammard ◽  
Marcello Giovannini ◽  
Claudio Galli ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Human Milk ◽  
Acid Composition ◽  
Saturated Fatty Acids ◽  
Total Fat ◽  
Extended Lactation ◽  
Long Chain

While a wealth of data on the fatty acid composition of mature human milk has been published, limited information is available on the quantities of individual fatty acids supplied to the suckling infant with maternal milk, through the whole first year of life. Our aim was to qualitatively and quantitatively evaluate the fatty acid composition of human milk from Italian mothers, throughout extended lactation with particular emphasis on the long-chain polyunsaturated fatty acids. We have thus measured the total fat content and the concentrations of major fatty acids by quantitative GLC in pooled breast hindmilk collected from all feedings over 24 h at colostrum, 1, 3, 6, 9 and 12 months in ten mothers recruited after delivery of full-term infants. Total saturated fatty acids progressively increase and total monounsaturated progressively decrease as percentage levels, while among long-chain polyunsaturated fatty acids, percentages of arachidonic acid and docosahexaenoic acid decrease from colostrum up to the third month. Hindmilk total lipids (mg/dl) rise more than twofold up to 3 months, and then remain stable. The amounts (mg/dl) of linoleic acid and α-linolenic acid progressively increase, following the trend of total fat, while arachidonic and docosahexaenoic concentrations (mg/dl) remain stable throughout the whole nursing period. Assessment of the intakes per kg body weight shows different trends for the individual major long-chain polyunsaturated fatty acids supplied to the infant from hindmilk during exclusive breast-feeding (3 months). This information may be useful for the evaluation of infant intakes during extended lactation.

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