Identification of anthocyanin compositions in black seed coated Korean adzuki bean (Vigna angularis) by NMR and UPLC-Q-Orbitrap-MS/MS and screening for their antioxidant properties using different solvent systems

2021 ◽  
Vol 346 ◽  
pp. 128882
Author(s):  
Tae Joung Ha ◽  
Jae Eun Park ◽  
Kwang-Sik Lee ◽  
Woo Duck Seo ◽  
Seok-Bo Song ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Adzuki Bean ◽  
Vigna Angularis ◽  
Black Seed ◽  
Solvent Systems ◽  
Orbitrap Ms

scholarly journals VaSDC1 Is Involved in Modulation of Flavonoid Metabolic Pathways in Black and Red Seed Coats in Adzuki Bean (Vigna angularis L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Liwei Chu ◽  
Pu Zhao ◽  
Kaili Wang ◽  
Bo Zhao ◽  
Yisong Li ◽  
...  
Keyword(s):  
Seed Coat ◽  
Metabolic Pathways ◽  
Nutritional Quality ◽  
Coat Colour ◽  
Myb Transcription Factor ◽  
Adzuki Bean ◽  
Vigna Angularis ◽  
Seed Coat Colour ◽  
Black Seed ◽  
Black Seed Coat

Seed coat colour is an important nutritional quality trait. Variations in anthocyanins and flavonoids induce the diversity of seed coat colour in adzuki bean (Vigna angularis L.). Red seed coat and black seed coat are important adzuki bean cultivars. Insights into the differences of flavonoid metabolic pathways between black and red adzuki bean are significant. In this study, we explored that the difference in seed coat colour between the red (Jingnong6) and the black (AG118) is caused by the accumulation of anthocyanins. The RNA-sequencing (RNA-Seq) and real-time reverse transcription (qRT)-PCR results showed that the Vigna angularis L. seed coat color (VaSDC1) gene, an R2R3-MYB transcription factor, should be the key gene to regulate the black and red seed coat colours. In three different colouring staes of seed development, VaSDC1 was specifically expressed in the black seed coat (AG118) landrace, which activates the structural genes of flavonoid metabolic pathways. As a result, this caused a substantial accumulation of anthocyanins and created a dark blue-black colour. In the red (Jingnong6) seed coat variety, low expression levels of VaSDC1 resulted in a lower accumulation of anthocyanins than in AG118. In addition, VaSDC1 was genetically mapped in the interval between simple-sequence repeat (SSR) markers Sca326-12, Sca326-4, and BAgs007 on chromosome 3 using an F4 segregating population derived from the cross between Jingnong6 and AG118. These results will facilitate the improvement of nutritional quality breeding in adzuki beans.

scholarly journals Stem Rot on Adzuki Bean (Vigna angularis) Caused by Rhizoctonia solani AG 4 HGI in China

2015 ◽  
Vol 31 (1) ◽  
pp. 67-71 ◽  
Author(s):  
Suli Sun ◽  
Changjian Xia ◽  
Jiqing Zhang ◽  
Canxing Duan ◽  
Xiaoming Wang ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Stem Rot ◽  
Adzuki Bean ◽  
Vigna Angularis

Isolation and Characterization of Adzuki Bean (Vigna angularis cv Takara) Proteins

1988 ◽  
Vol 53 (5) ◽  
pp. 1438-1443 ◽  
Author(s):  
CARMEN TJAHJADI ◽  
SHAOWEN LIN ◽  
WILLIAM M. BREENE
Keyword(s):  
Adzuki Bean ◽  
Vigna Angularis ◽  
Isolation And Characterization

Isolation and characterization of a cyanidin-catechin pigment from adzuki bean (Vigna angularis)

2013 ◽  
Vol 141 (1) ◽  
pp. 282-288 ◽  
Author(s):  
Umeo Takahama ◽  
Ryo Yamauchi ◽  
Sachiko Hirota
Keyword(s):  
Adzuki Bean ◽  
Vigna Angularis ◽  
Isolation And Characterization

scholarly journals UHPLC-HESI-OT-MS-MS Biomolecules Profiling, Antioxidant and Antibacterial Activity of the “Orange-Yellow Resin” from Zuccagnia punctata Cav.

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 123 ◽  
Author(s):  
Jessica Gómez ◽  
Mario J. Simirgiotis ◽  
Sofía Manrique ◽  
Beatriz Lima ◽  
Jorge Bórquez ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Liquid Chromatography ◽  
Metabolite Profiling ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Ultrahigh Resolution ◽  
Antioxidant Power ◽  
Orange Yellow ◽  
Ms Analysis ◽  
Orbitrap Ms

This research was designed to investigate the metabolite profiling, phenolics, and flavonoids content as well as the potential antioxidant and antibacterial, properties of orange-yellow resin from Zuccagnia punctata Cav (ZpRe). Metabolite profiling was obtained by a ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-ESI-OT-MS-MS). The antioxidant properties were screened by four methods: 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), trolox equivalent antioxidant activity assay (TEAC), ferric-reducing antioxidant power assay (FRAP), and lipid peroxidation in erythrocytes (LP)). The antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) rules. The resin displayed a strong DPPH scavenging activity (IC50 = 25.72 µg/mL) and showed a percentage of inhibition of LP close to that of the reference compound catechin (70% at 100 µg ZpRe/mL), while a moderated effect was observed in the FRAP and TEAC assays. The resin showed a content of phenolic and flavonoid compounds of 391 mg GAE/g and 313 mg EQ/g respectively. Fifty phenolics compounds were identified by ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-PDA-OT-MS) analysis. Thirty-one compounds are reported for the first time, updating the knowledge on the chemical profile of this species. The importance of the biomolecules identified support traditional use of this endemic plant. Furthermore, additional pharmacological data is presented that increase the potential interest of this plant for industrial sustainable applications.

scholarly journals Identification and Bioassay of Allelopathic Substances from Plant and Rhizosphere Soil Extracts of Adzuki Bean (Vigna angularis [Willd.] Ohwi & H. Ohashi.)

2016 ◽  
Vol 8 (11) ◽  
pp. 30 ◽  
Author(s):  
H. Song ◽  
P. P. Zhang ◽  
X. L. Gao ◽  
S. X. Xu ◽  
Q. Zhou ◽  
...  
Keyword(s):  
Seed Germination ◽  
Rhizosphere Soil ◽  
Adzuki Bean ◽  
Aqueous Extracts ◽  
Rhizospheric Soil ◽  
Vigna Angularis ◽  
Culture Dish ◽  
Soil Extracts ◽  
Whole Plant ◽  
The Impact

<p class="Abstract">The soil sickness of Adzuki bean [<em>Vigna angularis </em>(Willd.) Ohwi &amp; H. Ohashi] have been getting attention seriously under a continuously mono-cropping management-system in China. To investigate the allelopathy of rhizosphere soil and plant of Adzuki bean, culture dish and pot experiments were conducted. The compounds in the products were identified by High Performance Liquid Chromatography (HPLC).The aqueous extracts from rhizospheric soil and whole plant inhibited seed index (seed germination and seed potential) and plant growth under different degrees, and it showed concentration gradient effect on the inhibition of seed germination. Inhibition was stronger with increasment in extract concentration with the highest effect at 0.16 parts aqueous extract to 1 parts deionized water (0.16g·mL<sup>-1</sup>). The impact on growth varied among aqueous extracts from rhizospheric soil and the whole plant. Five phenolic compounds, cinnamic, phthalic, <em>p</em>-hydroxybenzoic, and isobutyricand glutaric acids, were detected from the rhizosphere soil and plant extract of Adzuki bean by HPLC method. Cinnamic, phthalic, and <em>p</em>-hydroxybenzoicacids were higher than that of the other phenolic acids. This result suggests that autotoxicity effects of phenolics on Adzuki bean probably happen in continuous mono-cropping systems.</p>

Preliminary study on geographical distribution and evolutionary relationships between cultivated and wild adzuki bean (Vigna angularis var. angularis and var. nipponensis) by AFLP analysis

2003 ◽  
Vol 1 (2-3) ◽  
pp. 175-183 ◽  
Author(s):  
Zong Xu-xiao ◽  
Duncan Vaughan ◽  
Norihiko Tomooka ◽  
Akito Kaga ◽  
Wang Xin-wang ◽  
...  
Keyword(s):  
Geographical Distribution ◽  
Nucleotide Diversity ◽  
Evolutionary Relationships ◽  
Aflp Analysis ◽  
Adzuki Bean ◽  
Asian Countries ◽  
Vigna Angularis ◽  
Aflp Data ◽  
Preliminary Study

AbstractA set of 146 representative adzuki (Vigna angularis var. angularis and var. nipponensis) germplasm from six Asian countries with a tradition of adzuki bean production, together with an outgroup standard rice bean (Vigna umbellata), were analysed by amplified fragment length polymorphism (AFLP) methodology using 12 informative primer pairs. A total of 313 unambiguous polymorphic bands were created. According to the dendrogram, using cluster analysis based on AFLP banding, 143 of the accessions were distinct and revealed enough genetic diversity for identification and classification of accessions within Vigna angularis. A neighbour-joining tree was generated using the newly developed Innan's nucleotide diversity estimate from the AFLP data. From analysis, seven distinct evolutionary groups, named ‘Chinese cultivated’, ‘Japanese cultivated’, ‘Japanese complex-Korean cultivated’, ‘Chinese wild’, ‘China Taiwan wild’, ‘Nepal- Bhutan cultivated’ and ‘Himalayan wild’, were detected. Nucleotide diversity with geographical distribution of each group is discussed, regarding the evolutionary relationships between wild and cultivated adzuki beans. The preliminary results indicated that cultivated adzuki beans have been domesticated from at least four progenitors with at least three geographical origins.

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