Encapsulation of phlorotannins from edible brown seaweed in chitosan: effect of fortification on bioactivity and stability in functional foods

2022 ◽  
pp. 132012
Author(s):  
K.G.D. Kaushalya ◽  
K.D.P.P. Gunathilake
2019 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Magdalena M. Stefaniak ◽  
María Gudjónsdóttir ◽  
Gudrun Marteinsdottir ◽  
Sesselja Omarsdottir ◽  
Elena Bravo ◽  
...  

Background: Sugar kelp (Saccharina latissima, formerly known as Laminaria saccharina) is a brown seaweed which naturally occurs in the North Atlantic. Seaweeds may be one of the last natural food resources abundantly available. They are known to contain many compounds which may have additional functional benefits. This edible seaweed is characterized by high content of nutrients including carbohydrates and polyphenols, which are recognized antioxidants. Because their natural environment is a 3% saline solution, they are very resistant to mild extraction methods. However, extracts from solvent extractions usually contain residual solvents, making them unacceptable for high quality functional foods and high end cosmetics. The objective of this study was to test the biological properties of three extracts from sugar kelp (Saccharina latissima).Methods: Cold water, hot water, and ethanol: water (70:30 v/v) extracts were prepared. Total Carbohydrate Content (TCC) was determined by the phenol – sulphuric acid method and values were expressed as mg of fucose/g of dry extract. Total Polyphenol Content (TPC) was determined and expressed as mg of Gallic Acid Equivalent (GAE)/100g of dry extract. Oxygen Radical Absorbance Capacity (ORAC) assay was performed for all extracts and values were expressed as µM of Trolox® Equivalent/g of dry extract. Human leukemia monocytic cell line (THP-1) was used to investigate the bioactivity of Saccharina extracts. Extracts were applied to PMA differentiated THP-1 cells. Cytotoxicity of derived extracts was assessed by light microscopy followed by XTT proliferation assay. Enzyme-linked Immunosorbent assays (ELISA) were performed to determine secretion of interleukin – 10 (IL-10), tumor necrosis factor – α (TNF-α) and interleukin – 6 (IL-6).Results: The cold water extract exhibited very toxic properties toward macrophages and was thereby excluded from the experimental proceedings with use of the macrophages. Among all the tested extracts, the hot water extract was richest in sugars (682±243 mg fucose/g dry extract) and polyphenols (96.5±5.6 mg GAE/g dry extract), which was correlated to the determined ORAC values (1686±99 μM TE/g dry extract). The addition of hot water and ethanol extracts at concentrations 100 μg/ml triggered secretion of pro-inflammatory cytokine TNF-α suggesting immunomodulatory properties of Saccharina extracts toward macrophages. Conclusions: The present study suggests that carbohydrate enriched extracts from Icelandic edible seaweed Saccharina latissima have antioxidant and immunomodulatory properties towards human THP–1 derived macrophages. The carbohydrate and polyphenol correlated with ORAC values confirming antioxidant properties of the derived extracts. The hot water extract affected the pro–inflammatory (TNF–α) and anti–inflammatory (IL–10) cytokine secretion in macrophages, suggesting their bioactivity through immunomodulatory actions and can be considered for practical applications in functional foods and cosmetics.Keywords: Seaweeds; Saccharina latissima; bioactivity; antioxidants; immunomodulation


2012 ◽  
Vol 82 (3) ◽  
pp. 144-147 ◽  
Author(s):  
Ibrahim Elmadfa ◽  
Alexa L. Meyer

A high-quality diet is one of the foundations of health and well-being. For a long time in human history, diet was chiefly a source of energy and macronutrients meant to still hunger and give the strength for work and activities that were in general much harder than nowadays. Only few persons could afford to emphasize enjoyment. In the assessment of quality, organoleptic properties were major criteria to detect spoilage and oxidative deterioration of food. Today, food hygiene is a quality aspect that is often taken for granted by consumers, despite its lack being at the origin of most food-borne diseases. The discovery of micronutrients entailed fundamental changes of the concept of diet quality. However, non-essential food components with additional health functions were still barely known or not considered important until recently. With the high burden of obesity and its associated diseases on the rise, affluent, industrialized countries have developed an increased interest in these substances, which has led to the development of functional foods to optimize special body functions, reduce disease risk, or even contribute to therapeutic approaches. Indeed, nowadays, high contents of energy, fat, and sugar are factors associated with a lower quality of food, and products with reduced amounts of these components are valued by many consumers. At the same time, enjoyment and convenience are important quality factors, presenting food manufacturers with the dilemma of reconciling low fat content and applicability with good taste and appealing appearance. Functional foods offer an approach to address this challenge. Deeper insights into nutrient-gene interactions may enable personalized nutrition adapted to the special needs of individuals. However, so far, a varied healthy diet remains the best basis for health and well-being.


2020 ◽  
Vol 653 ◽  
pp. 121-129
Author(s):  
RB Taylor ◽  
S Patke

Small mobile crustaceans are abundant on seaweeds. Many of these crustaceans rapidly abandon their host if it is detached from the seafloor and floats towards the surface, but the trigger for this ‘bailout’ behaviour is unknown. We tested 2 potential cues, i.e. rapid change in light and rapid change in water pressure, using >1 mm epifauna on the brown seaweed Carpophyllum plumosum as a model system. Bailout occurred in response to reduced water pressure, but not to changing light, as (1) bailout occurred at similar rates in light and dark, (2) bailout occurred on the seafloor when water pressure was reduced within a transparent chamber by the equivalent of ~0.5 m depth or more, and (3) little bailout occurred when water pressure was held constant within the chamber while seaweeds were raised to the surface. Increase in pressure (simulating sinking) did not induce bailout. The rate of bailout increased with increasing magnitude of pressure reduction but was not influenced greatly by the rate of change of pressure within the range tested (up to an equivalent of 0.4 m depth s-1). The use of pressure rather than light as a cue for bailout is consistent with the need for seaweed-associated crustaceans to rapidly abandon a detached host and relocate to suitable habitat during both day and night.


2017 ◽  
Vol 2017 ◽  
pp. 136-137
Author(s):  
Jenny Young ◽  
◽  
Denise Conroy ◽  
Sara Jaeger
Keyword(s):  

2017 ◽  
Vol 19 (3) ◽  
pp. 259-270
Author(s):  
M. Chesalin ◽  
S. Al-Ghassani ◽  
V. I. Ryabushko ◽  
N. I. Bobko ◽  
E. Gureeva ◽  
...  

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