Antimicrobial susceptibility, virulence gene and pulsed-field gel electrophoresis profiles of Salmonella enterica serovar Typhimurium recovered from retail raw chickens, China

Food Control ◽  
2017 ◽  
Vol 72 ◽  
pp. 36-42 ◽  
Author(s):  
Keting Li ◽  
Shuyao Ye ◽  
Walid Q. Alali ◽  
Yin Wang ◽  
Xin Wang ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Virulence Gene ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Serovar Typhimurium

scholarly journals Prevalence and Genetic Properties of Salmonella enterica Serovar Typhimurium Definitive Phage Type 104 Isolated from Rattus norvegicus and Rattus rattus House Rats in Yokohama City, Japan

2007 ◽  
Vol 73 (8) ◽  
pp. 2624-2630 ◽  
Author(s):  
Eiji Yokoyama ◽  
Soichi Maruyama ◽  
Hidenori Kabeya ◽  
Siro Hara ◽  
Shin Sata ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Phage Type ◽  
Rattus Rattus ◽  
Pulsed Field Gel Electrophoresis ◽  
Intestinal Contents ◽  
Serovar Typhimurium ◽  
J 24

ABSTRACT Salmonella enterica serovar Typhimurium was isolated from the intestinal contents of Rattus rattus and Rattus norvegicus house rats captured at two buildings, designated buildings J and YS, in Yokohama City, Japan. From October 1997 to September 1998, 52 of 339 (15.3%) house rats were found to carry Salmonella serovar Typhimurium definitive phage type 104 (DT104). In building J, 26 of 161 (16.1%) house rats carried DT104 over the 1-year study period, compared to 26 of 178 (14.6%) rats in building YS. The isolation rates of DT104 from R. rattus and R. norvegicus were similar in the two buildings. Most DT104 strains from building J (24 of 26) showed resistance to ampicillin, chloramphenicol, streptomycin, sulfisoxazole, and tetracycline and contained both the 1.0- and 1.2-kbp integrons, carrying genes pse1, pasppflo-like, aadA2, sulI, and tet(G). All DT104 strains from building YS were resistant to ampicillin and sulfisoxazole, and had the 1.2-kbp integron carrying pse1 and sulI. Cluster analysis of pulsed-field gel electrophoresis patterns of BlnI-digested DT104 DNAs showed that 22 of 26 DT104 strains from building J and 24 of 26 strains from building YS could be grouped into separate clusters each specific for the building origin. These results indicated that DT104 strains were prevalent in house rat colonies in each building and suggest that house rats may play an important role in the epidemiology of DT104.

scholarly journals Pulsed-Field Gel Electrophoresis Supports the Presence of Host-Adapted Salmonella enterica subsp. enterica Serovar Typhimurium Strains in the British Garden Bird Population

2011 ◽  
Vol 77 (22) ◽  
pp. 8139-8144 ◽  
Author(s):  
Becki Lawson ◽  
Laura A. Hughes ◽  
Tansy Peters ◽  
Elizabeth de Pinna ◽  
Shinto K. John ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Phage Type ◽  
Pulsed Field Gel Electrophoresis ◽  
Phage Typing ◽  
Molecular Technique ◽  
Content Type ◽  
Pulsed Field ◽  
Phage Types ◽  
Serovar Typhimurium

ABSTRACTSalmonellosis is a frequently diagnosed infectious disease of passerine birds in garden habitats within Great Britain with potential implications for human and domestic animal health. Postmortem examinations were performed on 1,477 garden bird carcasses of circa 50 species from England and Wales, 1999 to 2007 inclusive. Salmonellosis was confirmed in 263 adult birds of 10 passerine species in this 11-year longitudinal study. A subset of 124 fully biotypedSalmonella entericasubsp.entericaserovar Typhimurium isolates was examined using pulsed-field gel electrophoresis to investigate the hypothesis that these strains are host adapted and to determine whether this molecular technique offers greater resolution in understanding the epidemiology ofSalmonellaTyphimurium infection than phage typing alone. For the two most common phage types, definitive type (DT) 40 and DT56v, which together accounted for 97% (120/124) of isolates, pulsed-field gel electrophoresis groupings closely correlated with phage type with remarkably few exceptions. A high degree of genetic similarity (>90%) was observed within and between the two most common pulsed-field gel electrophoresis groups. No clustering or variation was found in the pulsed-field gel electrophoresis groupings by bird species, year, or geographical region beyond that revealed by phage typing. These findings support the hypothesis that there are currently two host-adaptedSalmonellaphage types,S. Typhimurium DT40 and DT56v, circulating widely in British garden birds and that the reservoir of infection is maintained within wild bird populations. Large-scale multilocus sequence typing studies are required to further investigate the epidemiology of this infection.

scholarly journals Fecal Excretion of Salmonella entericaSerovar Typhimurium Following a Food-Borne Outbreak

2000 ◽  
Vol 38 (9) ◽  
pp. 3495-3497 ◽  
Author(s):  
Toshiyuki Murase ◽  
Mikiko Yamada ◽  
Tetsunori Muto ◽  
Akiyoshi Matsushima ◽  
Shiro Yamai
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Pulsed Field Gel Electrophoresis ◽  
Fecal Excretion ◽  
Antimicrobial Drugs ◽  
Pulsed Field ◽  
Infected People ◽  
The People ◽  
Food Borne ◽  
Serovar Typhimurium

Fecal excretion of Salmonella enterica serovar Typhimurium organisms was observed in patients and in people not showing symptoms who were involved in an outbreak of food-borne infection with this organism. Excretion of organisms was prolonged in the patients who were given antimicrobial drugs compared with those who were not. The isolates were indistinguishable by their pulsed-field gel electrophoresis patterns and biotyping from the strain recovered from the roast pork that had been consumed by all of the people. This indicates that these isolates obtained from the infected people had originated in the contaminated pork.

scholarly journals Antimicrobial Susceptibility and Characterization of Salmonella Isolates from Processed Bison Carcasses

2006 ◽  
Vol 72 (4) ◽  
pp. 3046-3049 ◽  
Author(s):  
Qiongzhen Li ◽  
Jerod A. Skyberg ◽  
Mohamed K. Fakhr ◽  
Julie S. Sherwood ◽  
Lisa K. Nolan ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Virulence Genes ◽  
Pulsed Field Gel Electrophoresis ◽  
Cross Contamination ◽  
Class 1 Integron ◽  
Pulsed Field ◽  
Class 1

ABSTRACT Seventeen Salmonella enterica serovar Hadar isolates recovered from bison were found to possess a range of virulence genes and resistance to tetracycline, gentamicin, sulfamethoxazole, and streptomycin simultaneously. A 1-kb class 1 integron containing the aadA1 gene was identified in all isolates. Pulsed-field gel electrophoresis found that all isolates were closely related, indicating the possibility of cross-contamination during processing.

scholarly journals Molecular Epidemiology of Salmonella enterica Serovar Typhimurium Isolates Determined by Pulsed-Field Gel Electrophoresis: Comparison of Isolates from Avian Wildlife, Domestic Animals, and the Environment in Norway

2002 ◽  
Vol 68 (11) ◽  
pp. 5600-5606 ◽  
Author(s):  
Thorbjørn Refsum ◽  
Even Heir ◽  
Georg Kapperud ◽  
Traute Vardund ◽  
Gudmund Holstad
Keyword(s):  
Molecular Epidemiology ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Phage Type ◽  
Domestic Animals ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Source Of Infection ◽  
Serovar Typhimurium ◽  
A Minor

ABSTRACT The molecular epidemiology of 142 isolates of Salmonella enterica serovar Typhimurium from avian wildlife, domestic animals, and the environment in Norway was investigated using pulsed-field gel electrophoresis (PFGE) and computerized numerical analysis of the data. The bacterial isolates comprised 79 isolates from wild-living birds, including 46 small passerines and 26 gulls, and 63 isolates of nonavian origin, including 50 domestic animals and 13 environmental samples. Thirteen main clusters were discernible at the 90% similarity level. Most of the isolates (83%) were grouped into three main clusters. These were further divided into 20 subclusters at the 95% similarity level. Isolates from passerines, gulls, and pigeons dominated within five subclusters, whereas isolates from domestic animals and the environment belonged to many different subclusters with no predominance. The results support earlier results that passerines constitute an important source of infection to humans in Norway, whereas it is suggested that gulls and pigeons, based on PFGE analysis, represent only a minor source of human serovar Typhimurium infections. Passerines, gulls, and pigeons may also constitute a source of infection of domestic animals and feed plants or vice versa. Three isolates from cattle and a grain source, of which two were multiresistant, were confirmed as serovar Typhimurium phage type DT 104. These represent the first reported phage type DT 104 isolates from other sources than humans in Norway.

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