Virulence related traits in yeast species associated with food; Debaryomyces hansenii, Kluyveromyces marxianus, and Wickerhamomyces anomalus

Background: Yeasts play diverse roles in human life. Since ancient times, these micro organisms have been used to produce food products and beverages including bread and beer. Nowadays, the biotechnological products of yeast are some of the main components of commercial products. Objective: Some species of yeast such as Saccharomyces cerevisiae and Saccharomyces boulardii are recognized as probiotic yeast with extensive applications in the food and drug industries. However, certain species like Kluyveromyces marxianus are still not recognized as probiotic micro organisms despite their widespread industrial usage. In this study, the application of K. marxianus in preparing food and the medicinal product was reviewed in terms of its beneficial or harmful effects. Methods: Pub Med, Google Scholar, Scopus, and Science Direct databases were searched by using “Probiotics”, “Yeast”, and “Kluyveromyces marxianus”. Results: The findings suggest that K. marxianus can be recognized as a probiotic yeast species. Conclusion: It can be concluded that K. marxianus may be considered as a probiotic micro organism with a variety of commercial and medical applications.

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Screening For Yeast Phytase Leads to the Identification of a New Cell-Bound and Secreted Activity in Cyberlindnera jadinii CJ2

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.662598 ◽

2021 ◽

Vol 9 ◽

Author(s):

Claudia Capusoni ◽

Immacolata Serra ◽

Silvia Donzella ◽

Concetta Compagno

Keyword(s):

High Temperature ◽

Phytic Acid ◽

Convenient Method ◽

Kluyveromyces Marxianus ◽

Food Industry ◽

Yeast Species ◽

Industrial Applications ◽

Industrial Processes ◽

Acidic Ph ◽

Initial Screening

Phytic acid is an anti-nutritional compound able to chelate proteins and ions. For this reason, the food industry is looking for a convenient method which allows its degradation. Phytases are a class of enzymes that catalyze the degradation of phytic acid and are used as additives in feed-related industrial processes. Due to their industrial importance, our goal was to identify new activities that exhibit best performances in terms of tolerance to high temperature and acidic pH. As a result of an initial screening on 21 yeast species, we focused our attention on phytases found in Cyberlindnera jadinii, Kluyveromyces marxianus, and Torulaspora delbrueckeii. In particular, C. jadinii showed the highest secreted and cell-bound activity, with optimum of temperature and pH at 50°C and 4.5, respectively. These characteristics suggest that this enzyme could be successfully used for feed as well as for food-related industrial applications.

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Uranium removal from acidic aqueous solutions by Saccharomyces cerevisiae, Debaryomyces hansenii, Kluyveromyces marxianus and Candida colliculosa

Journal of Radioanalytical and Nuclear Chemistry ◽

10.1007/s10967-008-7274-8 ◽

2009 ◽

Vol 279 (3) ◽

pp. 709-711 ◽

Cited By ~ 19

Author(s):

S. Sarri ◽

P. Misaelides ◽

M. Papanikolaou ◽

D. Zamboulis

Keyword(s):

Saccharomyces Cerevisiae ◽

Aqueous Solutions ◽

Kluyveromyces Marxianus ◽

Debaryomyces Hansenii ◽

Uranium Removal

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Fungi in Rice Straw, Cane Straw, Maize Straw and Their Potential as Decomposer

SAINTEKBU ◽

10.32764/saintekbu.v10i1.161 ◽

2018 ◽

Vol 10 (1) ◽

pp. 39-54

Author(s):

Ika Rochdjatun Sastrahidayat ◽

Chintya Ivana Situmorang ◽

Anton Muhibuddin

Keyword(s):

Rice Straw ◽

Candida Tropicalis ◽

Organic Material ◽

Candida Parapsilosis ◽

Organic Fertilizer ◽

Debaryomyces Hansenii ◽

Material Decomposition ◽

Wickerhamomyces Anomalus ◽

Maize Straw

Organic material decomposition is the reorganizing process of the organic material by microbes in the controlled circumstances. The microbes which were used in general such as fungi, bacteria or yeast. Aerobic yeasts is one of the microbes needs oxygen to work. Yeast was obtained by the exploration of the rice straw, maize straw and cane straw in Dau, Malang, East of Java. By the result of the microscopic exploration and observation through the microscope, it was obtained 9 yeast isolates and 3 fungi isolates. 2 yeast isolates and 1 fungi isolate from the rice straw, 2 yeast isolates and 1 fungi isolate from the maize straw, and 5 yeast isolates and 1 fungi isolate from the cane straw. The identification results were obtained Candida parapsilosis, Bellera oryzae, Kluyveromyces thermotolerant, Candida tropicalis, Debaryomyces hansenii, Wickerhamomyces anomalus, Pichia membranfaciens, Cryptococcus wieringae. The highest potential yeast as an organic fertilizer decomposer is Cryptococcus wieringae and the lowest is Bullera oryzae. Keywords : Candida, Pichia, Cryptococcus, Bullera, Kluyveromyces, DebaryomycesWickerhamomyces, Fusarium and Trichoderma

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Several Yeast Species Induce Iron Deficiency Responses in Cucumber Plants (Cucumis sativus L.)

Microorganisms ◽

10.3390/microorganisms9122603 ◽

2021 ◽

Vol 9 (12) ◽

pp. 2603

Author(s):

Carlos Lucena ◽

María T. Alcalá-Jiménez ◽

Francisco J. Romera ◽

José Ramos

Keyword(s):

Yeast Species ◽

Reductase Activity ◽

Hansenula Polymorpha ◽

Debaryomyces Hansenii ◽

Root Hairs ◽

Fe Deficiency ◽

Ferric Reductase ◽

Cucumis Sativus L ◽

Growth System ◽

Promising Line

Iron (Fe) deficiency is a first-order agronomic problem that causes a significant decrease in crop yield and quality. Paradoxically, Fe is very abundant in most soils, mainly in its oxidized form, but is poorly soluble and with low availability for plants. In order to alleviate this situation, plants develop different morphological and physiological Fe-deficiency responses, mainly in their roots, to facilitate Fe mobilization and acquisition. Even so, Fe fertilizers, mainly Fe chelates, are widely used in modern agriculture, causing environmental problems and increasing the costs of production, due to the high prices of these products. One of the most sustainable and promising alternatives to the use of agrochemicals is the better management of the rhizosphere and the beneficial microbial communities presented there. The main objective of this research has been to evaluate the ability of several yeast species, such as Debaryomyces hansenii, Saccharomyces cerevisiae and Hansenula polymorpha, to induce Fe-deficiency responses in cucumber plants. To date, there are no studies on the roles played by yeasts on the Fe nutrition of plants. Experiments were carried out with cucumber plants grown in a hydroponic growth system. The effects of the three yeast species on some of the most important Fe-deficiency responses developed by dicot (Strategy I) plants, such as enhanced ferric reductase activity and Fe2+ transport, acidification of the rhizosphere, and proliferation of subapical root hairs, were evaluated. The results obtained show the inductive character of the three yeast species, mainly of Debaryomyces hansenii and Hansenula polymorpha, on the Fe-deficiency responses evaluated in this study. This opens a promising line of study on the use of these microorganisms as Fe biofertilizers in a more sustainable and environmentally friendly agriculture.

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Molekularna identifikacija kvasaca iz svježeg mlijeka i tradicionalno proizvedenog svježeg sira

Veterinarska stanica ◽

10.46419/vs.51.6.4 ◽

2020 ◽

Vol 51 (6) ◽

pp. 605-610

Author(s):

Manuela Zadravec ◽

Tomislav Mikuš ◽

Mario Mitak ◽

Maja Kiš ◽

Sanja Furmeg ◽

...

Keyword(s):

Candida Tropicalis ◽

Kluyveromyces Marxianus ◽

Debaryomyces Hansenii ◽

Rhodotorula Mucilaginosa ◽

Pichia Kudriavzevii ◽

Issatchenkia Orientalis

Kvasci su česti zagađivači mliječnih proizvoda, međutim, koriste se i kao starter kulture za poboljšavanje svojstava finalnog proizvoda. Njihova prisutnost u povećanom broju može prouzročiti nepoželjne promjene mliječnih proizvoda, kao što su neugodan miris, okus i izgled. Klasična identifikacija kvasaca na temelju morfoloških i biokemijskih svojstava spora je i nepouzdana. Cilj rada bio je identificirati kvasce iz 30 uzoraka mlijeka i svježeg sira te ustvrditi pripadaju li izolirani kvasci u poželjne ili nepoželjne vrste, odnosno predstavljaju li potencijalni rizik po zdravlje ljudi. Izolirani kvasci identificirani su sekvenciranjem D1/D2 regije gena 28 S rRNK. Dobivenim sekvencama ustvrđeno je da izolirani kvasci pripadaju vrstama: Kluyveromyces marxianus, Candida tropicalis, Trichosporon coremiiforme, Rhodotorula mucilaginosa, Trichosporon ovoides, Pichia kudriavzevii, Issatchenkia orientalis, koje se smatraju kvascima zagađenja te Debaryomyces hansenii vrsti koja se može koristiti i kao starter kultura. S obzirom da kvasci nisu termorezistentni mikroorganizmi, trebali bi biti uklonjeni tijekom pasterizacije, no u tradicionalnom načinu proizvodnje sira mlijeko se termički ne obrađuje, stoga je povećan rizik od pojavnosti kvasaca u finalnom proizvodu. Izolirane se vrste vrlo često nalaze i u okolišu i/ili na ljudima, odnosno životinjama. Stoga se nameće zaključak kako se glavnina kontaminacije kvascima događa kao posljedica neadekvatne higijenske prakse.

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A Chromogenic Medium for the Detection of Yeasts with β-Galactosidase and β-Glucosidase Activities from Intermediate Moisture Foods

Journal of Food Protection ◽

10.4315/0362-028x-63.5.651 ◽

2000 ◽

Vol 63 (5) ◽

pp. 651-654 ◽

Cited By ~ 13

Author(s):

M. ISABEL de SILÓNIZ ◽

MARÍA-JOSÉ VALDERRAMA ◽

JOSÉ M. PEINADO

Keyword(s):

Kluyveromyces Marxianus ◽

Solid Medium ◽

Debaryomyces Hansenii ◽

Chromogenic Substrates ◽

Specific Detection ◽

Chromogenic Medium ◽

Dark Blue

A selective and differential solid medium for the specific detection of some common yeasts frequently causing spoilage in intermediate moisture foods is described. The principle of the method is based on the detection of two enzymes, βglucosidase and β-galactosidase, using the chromogenic substrates salmon-Gluc and X-Gal. Over 140 yeasts and bacteria were tested, and Debaryomyces hansenii and Kluyveromyces marxianus strains produced salmon and dark blue colonies, respectively, thus permitting their clear discrimination from other yeasts common in intermediate moisture foods. The medium was very satisfactory when intermediate moisture foods were tested.

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Elevated minimum inhibitory concentrations to antifungal drugs prevail in 14 rare species of candidemia-causing Saccharomycotina yeasts

Medical Mycology ◽

10.1093/mmy/myaa005 ◽

2020 ◽

Vol 58 (7) ◽

pp. 987-995

Author(s):

Aimilia A Stavrou ◽

Antonio Pérez-Hansen ◽

Michaela Lackner ◽

Cornelia Lass-Flörl ◽

Teun Boekhout

Keyword(s):

Rare Species ◽

Yeast Species ◽

Antifungal Susceptibility ◽

Debaryomyces Hansenii ◽

Antifungal Drugs ◽

Antifungal Compound ◽

Candida Auris ◽

Minimum Inhibitory Concentrations ◽

Rare Yeast Species ◽

Multiple Drugs

Abstract Antifungal susceptibility profiles of rare Saccharomycotina yeasts remain missing, even though an increase in prevalence of such rare Candida species was reported in candidemia. Majority of these rare yeast species carry intrinsic resistances against at least one antifungal compound. Some species are known to be cross-resistant (against multiple drugs of the same drug class) or even multi-drug resistant (against multiple drugs of different drug classes). We performed antifungal susceptibility testing (AFST) according to EUCAST broth microdilution for 14 rare species (Clavispora lusitaniae, Candida intermedia, Candida auris, Diutina rugosa, Wickerhamiella pararugosa, Yarrowia lipolytica, Pichia norvegensis, Candida nivariensis, Kluyveromyces marxianus, Wickerhamomyces anomalus, Candida palmioleophila, Meyerozyma guilliermondii, Meyerozyma caribbica, and Debaryomyces hansenii) known to cause candidemia. In total, 234 isolates were tested for amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole, anidulafungin, micafungin, and caspofungin. Amphothericin B had the broadest efficiency against the 14 tested rare yeast species, while high minimum inhibitory concentrations (MICs) against azole drugs and echinocandins were common. Voriconazole was the most efficient azole drug. Multidrug resistance was observed for the species C. auris and K. marxianus. Multidrug resistant individual isolates were found for Y. lipolytica and M. caribbica. In conclusion, the observed high MIC values of the rare Saccharomycotina species tested limit antifungal treatment options, complicating the management of such infections.

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