Efficacy of saturated steam against Listeria innocua biofilm on common food-contact surfaces

Food Control ◽  
2021 ◽  
Vol 125 ◽  
pp. 107988
Author(s):  
Zi Hua ◽  
Frank Younce ◽  
Juming Tang ◽  
Dojin Ryu ◽  
Barbara Rasco ◽  
...  
Keyword(s):  
Listeria Innocua ◽  
Saturated Steam ◽  
Food Contact ◽  
Common Food ◽  
Food Contact Surfaces ◽  
Contact Surfaces

Control of Listeria innocua Biofilms on Food Contact Surfaces with Slightly Acidic Electrolyzed Water and the Risk of Biofilm Cells Transfer to Duck Meat

2018 ◽  
Vol 81 (4) ◽  
pp. 582-592 ◽  
Author(s):  
HYE RI JEON ◽  
MI JIN KWON ◽  
KI SUN YOON
Keyword(s):  
Stainless Steel ◽  
Food Processing ◽  
Biofilm Formation ◽  
Listeria Innocua ◽  
Potential Hazard ◽  
Processing Efficiency ◽  
Electrolyzed Water ◽  
Food Contact ◽  
Food Contact Surfaces ◽  
Contact Surfaces

ABSTRACT Biofilm formation on food contact surfaces is a potential hazard leading to cross-contamination during food processing. We investigated Listeria innocua biofilm formation on various food contact surfaces and compared the washing effect of slightly acidic electrolyzed water (SAEW) at 30, 50, 70, and 120 ppm with that of 200 ppm of sodium hypochlorite (NaClO) on biofilm cells. The risk of L. innocua biofilm transfer and growth on food at retail markets was also investigated. The viability of biofilms that formed on food contact surfaces and then transferred cells to duck meat was confirmed by fluorescence microscopy. L. innocua biofilm formation was greatest on rubber, followed by polypropylene, glass, and stainless steel. Regardless of sanitizer type, washing removed biofilms from polypropylene and stainless steel better than from rubber and glass. Among the various SAEW concentrations, washing with 70 ppm of SAEW for 5 min significantly reduced L. innocua biofilms on food contact surfaces during food processing. Efficiency of transfer of L. innocua biofilm cells was the highest on polypropylene and lowest on stainless steel. The transferred biofilm cells grew to the maximum population density, and the lag time of transferred biofilm cells was longer than that of planktonic cells. The biofilm cells that transferred to duck meat coexisted with live, injured, and dead cells, which indicates that effective washing is essential to remove biofilm on food contact surfaces during food processing to reduce the risk of foodborne disease outbreaks.

Generation of Airborne Listeria innocua from Model Floor Drains†

2012 ◽  
Vol 75 (7) ◽  
pp. 1328-1331 ◽  
Author(s):  
MARK E. BERRANG ◽  
JOSEPH F. FRANK
Keyword(s):  
Tap Water ◽  
Listeria Innocua ◽  
Cross Contamination ◽  
Food Contact ◽  
Food Contact Surfaces ◽  
Selective Agar ◽  
Poultry Processing ◽  
Contact Surfaces ◽  
Cleaning And Disinfection ◽  
Phosphate Buffered Saline

Listeria monocytogenes can colonize floor drains in poultry processing and further processing facilities, remaining present even after cleaning and disinfection. Therefore, during wash down, workers exercise caution to avoid spraying hoses directly into drains in an effort to prevent the escape and transfer of drain microflora to food contact surfaces. The objective of this study was to examine the extent to which an inadvertent water spray into a colonized floor drain can cause the spread of airborne Listeria. Listeria innocua was used to inoculate a polyvinyl chloride model floor drain, resulting in approximately 108 cells per ml of phosphate-buffered saline and 104 attached cells per square centimeter of inner surface. Each model drain was subjected to a 2-s spray of tap water at 68.9 kPa from a distance of 1 m. Drains were sprayed while filled and again after emptying. Airborne cells were collected by using sedimentation plates containing Listeria selective agar which were placed on the floor and walls of a contained room at incremental horizontal and vertical distances of 0.6, 1.2, 2.4, or 4.0 m from the drain. Sedimentation plates were exposed for 10 min. A mechanical sampler was used to also collect air by impaction on the surface of Listeria selective agar to determine the number of cells per liter of air. The experiment was conducted in triplicate rooms for each of four replications. L. innocua was detected on sedimentation plates on the floor as far as 4.0 m from the drain and on walls as high as 2.4 m above the floor and 4 m from the drain. A 2-s spray with a water hose into a contaminated drain can cause airborne spread of Listeria, resulting in the potential for cross-contamination of food contact surfaces, equipment, and exposed product.

scholarly journals Genetic Listeria monocytogenes Types in the Pork Processing Plant Environment: From Occasional Introduction to Plausible Persistence in Harborage Sites

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 717
Author(s):  
Niels Demaître ◽  
Geertrui Rasschaert ◽  
Lieven De Zutter ◽  
Annemie Geeraerd ◽  
Koen De Reu
Keyword(s):  
Genetic Diversity ◽  
Environmental Samples ◽  
Genetic Characterization ◽  
Processing Plant ◽  
Pfge Analysis ◽  
Food Contact ◽  
Plant Environment ◽  
Food Contact Surfaces ◽  
Contact Surfaces ◽  
Cleaning And Disinfection

The purpose of this study was to investigate the L. monocytogenes occurrence and genetic diversity in three Belgian pork cutting plants. We specifically aim to identify harborage sites and niche locations where this pathogen might occur. A total of 868 samples were taken from a large diversity of food and non-food contact surfaces after cleaning and disinfection (C&D) and during processing. A total of 13% (110/868) of environmental samples tested positive for L. monocytogenes. When looking in more detail, zone 3 non-food contact surfaces were contaminated more often (26%; 72/278) at typical harborage sites, such as floors, drains, and cleaning materials. Food contact surfaces (zone 1) were less frequently contaminated (6%; 25/436), also after C&D. PFGE analysis exhibited low genetic heterogeneity, revealing 11 assigned clonal complexes (CC), four of which (CC8, CC9, CC31, and CC121) were predominant and widespread. Our data suggest (i) the occasional introduction and repeated contamination and/or (ii) the establishment of some persistent meat-adapted clones in all cutting plants. Further, we highlight the importance of well-designed extensive sampling programs combined with genetic characterization to help these facilities take corrective actions to prevent transfer of this pathogen from the environment to the meat.

scholarly journals UV-C LED Irradiation Reduces Salmonella on Chicken and Food Contact Surfaces

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1459
Author(s):  
Alexandra Calle ◽  
Mariana Fernandez ◽  
Brayan Montoya ◽  
Marcelo Schmidt ◽  
Jonathan Thompson
Keyword(s):  
Light Emitting Diode ◽  
Chicken Breast ◽  
Light Emitting ◽  
Work Surface ◽  
Food Contact ◽  
Bacterial Aggregation ◽  
Food Contact Surfaces ◽  
Contact Surfaces ◽  
Uv C ◽  
Surface Pores

Ultraviolet (UV-C) light-emitting diode (LED) light at a wavelength of 250–280 nm was used to disinfect skinless chicken breast (CB), stainless steel (SS) and high-density polyethylene (HD) inoculated with Salmonella enterica. Irradiances of 2 mW/cm2 (50%) or 4 mW/cm2 (100%) were used to treat samples at different exposure times. Chicken samples had the lowest Salmonella reduction with 1.02 and 1.78 Log CFU/cm2 (p ≤ 0.05) after 60 and 900 s, respectively at 50% irradiance. Higher reductions on CB were obtained with 100% illumination after 900 s (>3.0 Log CFU/cm2). Salmonella on SS was reduced by 1.97 and 3.48 Log CFU/cm2 after 60 s of treatment with 50% and 100% irradiance, respectively. HD showed a lower decrease of Salmonella, but still statistically significant (p ≤ 0.05), with 1.25 and 1.77 Log CFU/cm2 destruction for 50 and 100% irradiance after 60 s, respectively. Longer exposure times of HD to UV-C yielded up to 99.999% (5.0 Log CFU/cm2) reduction of Salmonella with both irradiance levels. While UV-C LED treatment was found effective to control Salmonella on chicken and food contact surfaces, we propose three mechanisms contributing to reduced efficacy of disinfection: bacterial aggregation, harboring in food and work surface pores and light absorption by fluids associated with CB.

Rapid Method for Detection and Quantitation of Lipid Soils on Food Contact Surfaces: Evaluation of a Model System

1980 ◽  
Vol 43 (6) ◽  
pp. 447-449 ◽  
Author(s):  
K. E. EUGSTER ◽  
B. J. SKURA ◽  
W. D. POWRIE
Keyword(s):  
Rapid Method ◽  
Peak Height ◽  
Model System ◽  
Polyethylene Film ◽  
Infrared Spectrophotometry ◽  
Food Contact ◽  
Food Contact Surfaces ◽  
Board Material ◽  
Food Contact Surface ◽  
Contact Surfaces

A rapid method for detection and quantitation of lipid-containing food soils on food-contact surfaces has been developed to ascertain whether these surfaces have been properly cleaned. The method is based on transfer of lipid-based soils from a food-contact surface to a polyethylene film and subsequent quantitation of the lipid, at 1750 cm−1, by infrared spectrophotometry. Peak height at 1750 cm−1 is linearly related to the quantity of lipid on the polyethylene surface. Standard curves for peak-height against lipid distribution on the polyethylene film were constructed for stainless steel, glass and three types of plastic cutting board material (high density polyethylene, smooth nylotrol and rough nylotro).

Efficacy of Commonly Used Disinfectants for the Inactivation of Calicivirus on Strawberry, Lettuce, and a Food-Contact Surface

2001 ◽  
Vol 64 (9) ◽  
pp. 1430-1434 ◽  
Author(s):  
BALDEV R. GULATI ◽  
PAUL B. ALLWOOD ◽  
CRAIG W. HEDBERG ◽  
SAGAR M. GOYAL
Keyword(s):  
Phenolic Compounds ◽  
Fruits And Vegetables ◽  
Virus Titer ◽  
Ammonium Compound ◽  
Food Contact ◽  
Food Contact Surfaces ◽  
Environmental Surfaces ◽  
Foodborne Outbreaks ◽  
Food Contact Surface ◽  
Contact Surfaces

Norwalk and Norwalk-like viruses (NLVs) are important causes of foodborne gastroenteritis in restaurant-related outbreaks. Efficacy of common disinfection methods against these viruses on food-contact surfaces and fresh produce is not known partially because of their nonculturability. Seven commercial disinfectants for food-contact surfaces and three sanitizers for fruits and vegetables were tested against cultivable feline calicivirus (FCV). Disks of stainless steel, strawberry, and lettuce were contaminated with known amounts of FCV. The disinfectants were applied at one, two, and four times the manufacturer's recommended concentrations for contact times of 1 and 10 min. The action of disinfectant was stopped by dilution, and the number of surviving FCVs was determined by titration in cell cultures. An agent was considered effective if it reduced the virus titer by at least 3 log10 from an initial level of 107 50% tissue culture infective dose. None of the disinfectants was effective when used at the manufacturer's recommended concentration for 10 min. Phenolic compounds, when used at two to four times the recommended concentration, completely inactivated FCV on contact surfaces. A combination of quaternary ammonium compound and sodium carbonate was effective on contact surfaces at twice the recommended concentration. Rinsing of produce with water alone reduced virus titer by 2 log10. On artificially contaminated strawberry and lettuce, peroxyacetic acid and hydrogen peroxide was the only effective formulation when used at four times the manufacturers' recommended concentration for 10 min. These findings suggest that FCV and perhaps NLVs are very resistant to commercial disinfectants. However, phenolic compounds at two to four times their recommended concentrations appear to be effective at decontaminating environmental surfaces and may help control foodborne outbreaks of calicivirus in restaurants.

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