Antibacterial mechanism of linalool against L. monocytogenes, a metabolomic study

Abstract Background The deregulation of immune system cell response implies loss of T-cell apoptosis, high rate of proinflammatory cytokines production and subsequent exacerbate activation of TNF-α pathway. The use of biologic antibody decrease inflammation rate and symptoms, but it remains unclear if it has a direct effect on the pathways activation/inactivation on peripheral blood mononuclear cells (PBMCs). The aim of this study is evaluate the role of nuclear magnetic resonance spectroscopy (NMR) applied to the metabolomic study of serum samples isolated from fresh blood from inflammatory bowel disease (IBD) patients under IFX treatment to understand the activated/inactivated pathways of PBMCs. Methods A case–control study was performed. Inclusion criteria were IBD patients under IFX treatment. Blood samples were obtained in Crohn’s disease (CD) and ulcerative colitis (UC) patients before IFX and in healthy controls (CTRL). CD patients were divided into subgroups according to the gut affected, in Ileocolic (IC), ileum and colon. NMR samples of the serum were collected and measured according to Standard Operation Procedures. Three types of NMR spectra were measured for each serum sample (1Hnoepresat, 1Hcpmgpresat and 1HDfilterpresat). The signal in each NMR spectrum was integrated in a series of equidistant little portion of the spectrum called buckets of a constant width of 0.04 ppm, covering the complete 1H NMR spectral window from −5 to 14 ppm. Buckets in regions depleted from signal at the two extremes of the spectrum were discarded as well as those in the proximity of the water peak at ca. 4.7 ppm which was affected by the presaturation. The vectors corresponding to a number of samples of two or more groups can be rapidly analysed using Multivariant Statistical Analysis methods. Results Twenty-two IBD patients (12 CD and nine UC) were included, 10 CTRL were also included. The metabolomic analyses of the NMR spectra of the serum of the different patients and control groups by the fingerprinting and targeting profiling strategies provided OPLS-DA statistical models (Figure 1) that permitted the successful classification of certain groups of samples which are summarised in Table 1. Conclusion The results of this pilot NMR metabolomic study of serum samples of IBD found a series of spectral fingerprints that are able to discriminate between groups of patients CTRL and CD, which underlines its potential use for the diagnosis of the disease.

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Fluorescent Amphiphilic Quaternized β-Chitin: Antibacterial Mechanism and Cell Imaging

ACS Applied Bio Materials ◽

10.1021/acsabm.1c00179 ◽

2021 ◽

Author(s):

Huan Xu ◽

Fang Xie ◽

Yiwen Lu ◽

Pingdong Wei ◽

Jie Cai

Keyword(s):

Cell Imaging ◽

Antibacterial Mechanism

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Antibacterial activity and antibacterial mechanism of ethanol extracts of Lentinula edodes (Shiitake) and Agaricus bisporus (button mushroom)

International Journal of Environmental Health Research ◽

10.1080/09603123.2021.1919292 ◽

2021 ◽

pp. 1-14

Author(s):

Elif Ayşe Erdoğan Eliuz

Keyword(s):

Antibacterial Activity ◽

Agaricus Bisporus ◽

Lentinula Edodes ◽

Antibacterial Mechanism ◽

Button Mushroom ◽

Ethanol Extracts

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Antibacterial mechanism of rhodomyrtone involves the disruption of nucleoid segregation checkpoint in Streptococcus suis

AMB Express ◽

10.1186/s13568-020-01047-x ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Apichaya Traithan ◽

Pongsri Tongtawe ◽

Jeeraphong Thanongsaksrikul ◽

Supayang Voravuthikunchai ◽

Potjanee Srimanote

Keyword(s):

Streptococcus Suis ◽

Antibacterial Mechanism

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Chitosan based antibacterial composite materials for leather industry: a review

Journal of Leather Science and Engineering ◽

10.1186/s42825-020-00045-w ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Linlin Yuan ◽

Qingda Yao ◽

Yongxian Liang ◽

Ye Dan ◽

Yixiao Wang ◽

...

Keyword(s):

Extracellular Matrix ◽

Polyethylene Glycol ◽

Composite Materials ◽

Water Treatment ◽

Future Development ◽

Structural Unit ◽

Important Application ◽

Antibacterial Mechanism ◽

Leather Industry ◽

Preparation Methods

Abstract Chitosan is an amorphous translucent substance with a structural unit similar to the polysaccharide structure of the extracellular matrix, It has good antibacterial, biocompatible, and degradable properties. It has important application value in leather, water treatment, medicine, food and other fields, so chitosan and its modified products have received widespread attention. This article reviewed the preparation methods of chitosan-based antibacterial composites in recent years, including chitosan/collagen, chitosan/graphene, chitosan/tannic acid, and chitosan/polyethylene glycol composite materials, elaborates their modification methods and antibacterial mechanism were reviewed in detail, and its applications in the leather industry as antibacterial auxiliaries and water treatment antibacterial adsorption materials were discussed. Finally, the future development and challenges of chitosan-based composite materials in the leather industry were forecasted. Graphical abstract

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