Stabilization of the air-liquid interface in sponge cake batter by surface-active proteins and lipids: A foaming protocol based approach

2020 ◽  
Vol 101 ◽  
pp. 105548 ◽  
Author(s):  
Sarah C. Pycarelle ◽  
Geertrui M. Bosmans ◽  
Hélène Nys ◽  
Kristof Brijs ◽  
Jan A. Delcour
2021 ◽  
Vol 140 ◽  
pp. 110007
Author(s):  
Sarah C. Pycarelle ◽  
Geertrui M. Bosmans ◽  
Bram Pareyt ◽  
Kristof Brijs ◽  
Jan A. Delcour

Foods ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 107
Author(s):  
Sarah C. Pycarelle ◽  
Geertrui M. Bosmans ◽  
Bram Pareyt ◽  
Kristof Brijs ◽  
Jan A. Delcour

The main sponge cake ingredients are flour, sucrose, eggs and leavening agents. Exogenous lipids (e.g., monoacylglycerols) are often used to increase air–liquid interface stability in the batter. There is a consumer trend to avoid foods containing such additives. We here reasoned that egg yolk may be an alternative source of surface-active lipids and set out to study the role of egg yolk lipids during sponge cake making. This was done by relocating or removing them prior to batter preparation using ethanol treatments and examining how this affects cake (batter) properties and structure setting during baking. Most egg yolk lipids occur within spherical low-density lipoproteins (LDLs) which were disintegrated by the ethanol treatments. Results showed that egg yolk lipids impact air–liquid interface stability and less so cake structure setting. To prepare high-quality sponge cakes by multistage mixing preferably intact LDLs or, alternatively, their components are needed to incorporate sufficient air during mixing and to stabilize it after mixing. It was also shown that the batter contains intact LDLs in the continuous phase and disintegrated LDLs at air–liquid interfaces. Sponge cake contains intact LDLs in the cake matrix, disintegrated LDLs at air–crumb interfaces and disintegrated LDLs incorporated into the protein network.


Pneumologie ◽  
2011 ◽  
Vol 65 (12) ◽  
Author(s):  
M Selmansberger ◽  
AG Lenz ◽  
M Schmidmeir ◽  
O Eickelberg ◽  
T Stoeger ◽  
...  

2020 ◽  
Author(s):  
S. Runft ◽  
L. Burigk ◽  
A. Lehmbecker ◽  
K. Schöne ◽  
D. Waschke ◽  
...  

2021 ◽  
Vol 33 (6) ◽  
pp. 062103
Author(s):  
Meisam Pourali ◽  
Martin Kröger ◽  
Jan Vermant ◽  
Patrick D. Anderson ◽  
Nick O. Jaensson

Langmuir ◽  
2021 ◽  
Author(s):  
Jin Tae Park ◽  
Govind Paneru ◽  
Masao Iwamatsu ◽  
Bruce M. Law ◽  
Hyuk Kyu Pak

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2639
Author(s):  
Frauke Stanke ◽  
Sabina Janciauskiene ◽  
Stephanie Tamm ◽  
Sabine Wrenger ◽  
Ellen Luise Raddatz ◽  
...  

The cystic fibrosis transmembrane conductance regulator (CFTR) gene is influenced by the fundamental cellular processes like epithelial differentiation/polarization, regeneration and epithelial–mesenchymal transition. Defects in CFTR protein levels and/or function lead to decreased airway surface liquid layer facilitating microbial colonization and inflammation. The SERPINA1 gene, encoding alpha1-antitrypsin (AAT) protein, is one of the genes implicated in CF, however it remains unknown whether AAT has any influence on CFTR levels. In this study we assessed CFTR protein levels in primary human lung epithelial cells grown at the air-liquid-interface (ALI) alone or pre-incubated with AAT by Western blots and immunohistochemistry. Histological analysis of ALI inserts revealed CFTR- and AAT-positive cells but no AAT-CFTR co-localization. When 0.5 mg/mL of AAT was added to apical or basolateral compartments of pro-inflammatory activated ALI cultures, CFTR levels increased relative to activated ALIs. This finding suggests that AAT is CFTR-modulating protein, albeit its effects may depend on the concentration and the route of administration. Human lung epithelial ALI cultures provide a useful tool for studies in detail how AAT or other pharmaceuticals affect the levels and activity of CFTR.


Sign in / Sign up

Export Citation Format

Share Document