Improving the application of SSR polymorphism analysis coupled with Lab-on-a-chip® capillary electrophoresis to assess food authenticity: Italian pigmented rice as case study

2014 ◽  
Vol 64 ◽  
pp. 790-798 ◽  
Author(s):  
Cristiano Garino ◽  
Maurizio Rinaldi ◽  
Angelo de Paolis ◽  
Fabiano Travaglia ◽  
Jean Daniel Coïsson ◽  
...  
Author(s):  
Annemieke M. Pustjens ◽  
Rita Boerrigter-Eenling ◽  
Alex H. Koot ◽  
Saskia M. van Ruth
Keyword(s):  

2015 ◽  
Vol 17 (11) ◽  
pp. 1941-1951 ◽  
Author(s):  
Hong Anh Duong ◽  
Minh Duc Le ◽  
Kim Diem Mai Nguyen ◽  
Peter C. Hauser ◽  
Hung Viet Pham ◽  
...  

A simple and inexpensive approach for the determination of ionic species is reported. Various inorganic cations and anions in different water matrices in Vietnam were determined using purpose-made CE-C4D instruments.


2005 ◽  
Vol 26 (7-8) ◽  
pp. 1389-1397 ◽  
Author(s):  
Carolin Huhn ◽  
Christian Neusüß ◽  
Matthias Pelzing ◽  
Ute Pyell ◽  
Joachim Mannhardt ◽  
...  

2005 ◽  
Vol 59 (3) ◽  
pp. 335-339 ◽  
Author(s):  
Tara Morcone Snyder ◽  
Linda B. McGown

This paper describes the use of on-the-fly fluorescence lifetime detection (OFLD) for multiplex single strand conformation polymorphism (SSCP) analysis by capillary electrophoresis (CE). The dye labels studied for multiplex SSCP-OFLD-CE analyses included RG, NBD, and BODIPY-FL. The dyes were first investigated for a model system of “Wild Type” and “Mutant” 43-base fragments designed to vary by a single A/T substitution. Two dye pairs, BODIPY-FL/RG and BODIPY-FL/NBD, were then used to detect the G20210A mutation in the human prothrombin gene. Mobility correction was required for the BODIPY-FL/RG system. Three “blind” analyses were performed of three mixtures that combined a control fragment (wild type-BODIPY-FL) with two “unknown” fragments selected among four possibilities (wild type or mutant labeled with NBD or RG). In each multiplex analysis, the “origin” of the unknown fragments was correctly identified on the basis of fluorescence lifetime of the dye label and the presence or absence of the mutation was correctly determined on the basis of conformation-induced differences in migration time.


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