scholarly journals Systemic lupus erythematosus onset in lupus-prone B6.MRL/lpr mice Is influenced by weight gain and Is preceded by an increase in neutrophil oxidative burst activity

2015 ◽  
Vol 86 ◽  
pp. 362-373 ◽  
Author(s):  
Juliana Escher Toller-Kawahisa ◽  
Nathália Cristina Canicoba ◽  
Vinicius Paula Venancio ◽  
Rogério Kawahisa ◽  
Lusânia Maria Greggi Antunes ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Weight Gain ◽  
Lupus Erythematosus ◽  
Oxidative Burst ◽  
Burst Activity ◽  
Systemic Lupus ◽  
Oxidative Burst Activity ◽  
Neutrophil Oxidative Burst

Combination of Autoantibodies Against Different Histone Proteins Influences Complement-dependent Phagocytosis of Necrotic Cell Material by Polymorphonuclear Leukocytes in Systemic Lupus Erythematosus

2012 ◽  
Vol 39 (8) ◽  
pp. 1619-1627 ◽  
Author(s):  
BIRGITTA GULLSTRAND ◽  
MALIN H. LEFORT ◽  
HELENA TYDÉN ◽  
ANDREAS JÖNSEN ◽  
CHRISTIAN LOOD ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Oxidative Burst ◽  
Polymorphonuclear Leukocytes ◽  
Burst Activity ◽  
Classical Pathway ◽  
Necrotic Cell ◽  
Systemic Lupus ◽  
Histone Proteins ◽  
Oxidative Burst Activity

Objective.Polymorphonuclear leukocytes (PMN) with autoantibody-coated engulfed necrotic cell material (NC) are frequently seen in systemic lupus erythematosus (SLE). We evaluated the roles of complement, different antihistone antibodies (anti-H ab), and oxidative burst in the phagocytosis of NC by PMN, as well as association to disease activity and clinical phenotype in SLE.Methods.ELISA and immunoblot were used to measure antibodies to different histone proteins in sera from patients with SLE and complement-deficient individuals. Phagocytosis of NC by PMN and oxidative burst activity was assessed by flow cytometry.Results.A clearly increased phagocytosis of NC was seen in patients with active SLE, which was associated with high levels of anti-H ab concentrations and oxidative burst activity. The complement system contributed to efficient phagocytosis of NC by PMN through activation of the classical pathway, and the phagocytosis was mediated by FcγRIIA, FcγRIIIB, and CR1 in combination. A pattern of high phagocytosis, consumption of classical pathway components, and a broad anti-H ab repertoire was seen particularly in patients with nephritis and serositis. The combination of antibodies to several different histone proteins, often with anti-DNA antibodies, promoted an efficient uptake of NC, whereas antibodies against only histone H1 or a few histones seemed to be of less importance.Conclusion.The distributions of specificities among anti-H ab are of great importance in the complement-dependent phagocytosis of debris from NC in SLE. Measurement of anti-H ab could be useful in monitoring of this disease and contribute to improved understanding of the autoimmune process.

Increased neutrophil oxidative burst metabolism in systemic lupus erythematosus

Lupus ◽  
2012 ◽  
Vol 21 (14) ◽  
pp. 1543-1551 ◽  
Author(s):  
SF Perazzio ◽  
R Salomão ◽  
NP Silva ◽  
LEC Andrade
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Oxidative Burst ◽  
Systemic Lupus ◽  
Neutrophil Oxidative Burst

scholarly journals Gestational weight gain in women with systemic lupus erythematosus

Lupus ◽  
2016 ◽  
Vol 26 (6) ◽  
pp. 623-632 ◽  
Author(s):  
A M Eudy ◽  
A M Siega-Riz ◽  
S M Engel ◽  
N Franceschini ◽  
A G Howard ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Body Mass Index ◽  
Weight Gain ◽  
Gestational Weight Gain ◽  
Body Mass ◽  
Lupus Erythematosus ◽  
Institute Of Medicine ◽  
Systemic Lupus ◽  
Gestational Weight ◽  
Excessive Gestational Weight Gain

Objective The objective of this study was to estimate the proportion of pregnant women with systemic lupus erythematosus meeting Institute of Medicine guidelines for gestational weight gain and determine correlates of adherence to guidelines. Methods Singleton, live births in the Hopkins Lupus Pregnancy Cohort 1987–2015 were included. Pre-pregnancy weight was the weight recorded 12 months prior to pregnancy/first trimester. Final weight was the last weight recorded in the third trimester. Adherence to Institute of Medicine guidelines (inadequate, adequate, or excessive) was based on pre-pregnancy body mass index. Fisher’s exact test and analysis of variance determined factors associated with not meeting guidelines. Stepwise selection estimated predictors of gestational weight gain. Results Of the 211 pregnancies, 34%, 24% and 42% had inadequate, adequate and excessive gestational weight gain, respectively. In exploratory analyses, differences in Institute of Medicine adherence were observed by pre-pregnancy body mass index, race, elevated creatinine during pregnancy and pre-pregnancy blood pressure. Odds of inadequate and excessive gestational weight gain increased 12% with each 1 kg/m2 increase in pre-pregnancy body mass index. Lower maternal education was associated with increased odds of inadequate and excessive gestational weight gain. Conclusions As in the general population, most women with systemic lupus erythematosus did not meet Institute of Medicine guidelines. Our results identified predictors of gestational weight gain to aid in targeted interventions to improve guideline adherence in this population.

scholarly journals DRUG USE EVALUATION OF CORTICOSTEROIDSIN SYSTEMIC LUPUS ERYTHEMATOSUS OUTPATIENTS

2018 ◽  
Vol 1 (1) ◽  
pp. 11-15
Author(s):  
M.Caecilia N.Setiawati
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Peptic Ulcer ◽  
Weight Gain ◽  
Drug Use ◽  
Lupus Erythematosus ◽  
Inclusion Criteria ◽  
Systemic Lupus ◽  
Cross Sectional ◽  
Cross Sectional Design ◽  
Per Oral

Corticosteroids was the mainstay therapy for SLE, an autoimmune chronic desease. Prolonged use of Corticosteroid caused many side effects’ risk. This study was aimed to evaluate corticosteroid drug use in SLE outpatients. This study was conducted on April 2017 to August 2017. Cross sectional design was used and presented with analytical descriptive. The result is, 192 SLE outpatients pass the inclusion criteria and 98,96% are women. Methylprednisolon per oral is the most corticosteroid used for SLE outpatients, combine with 1 other immnunsuppresant ( 65,10  %) and combine with 2 other immunsuppresant ( 8,33% ). From  BMI, 26,05% subjects are overweight, perhaps because of corticosteroids side effect, such as weight gain. More than a half SLE outpatients (58,33%) get Calcium supplement and vitamin D3 as prophylactive for osteoporosis because of prolonged use of  corticosteroid and  61 SLE outpatients get drug for gastrointestinal use to avoid peptic ulcer. There are   44  cases of  potential corticosteroid interaction with other drug that are given together, the most are corticosteroid and aspirin interaction, on 14 patients.

scholarly journals The impact of neutrophil extracellular trap from patients with systemic lupus erythematosus on the viability, CD11b expression and oxidative burst of healthy neutrophils

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Alimohammad Fatemi ◽  
Razieh Alipour ◽  
Hossein Khanahmad ◽  
Fereshteh Alsahebfosul ◽  
Alireza Andalib ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Oxidative Burst ◽  
Neutrophil Extracellular Trap ◽  
Systemic Lupus ◽  
Cd11b Expression ◽  
Cell Mortality ◽  
Extracellular Trap ◽  
The Impact

Abstract Background NET (neutrophil extracellular trap) has been shown to directly influence inflammation; in SLE (systemic lupus erythematosus), it is reportedly a plausible cause for the broken self-tolerance that contributes to this pathology. Meanwhile, the role of NET is not easily explicable, and there is a serious discrepancy in the role of NET in SLE pathology and generally inflammation; in particular, the interactions of neutrophils with NET have been rarely inspected. This study evaluates the effect of NET on neutrophils in the context of SLE. The neutrophils were incubated by the collected NET (from SLE patients and healthy controls) and their expression of an activation marker, viability and oxidative burst ability were measured. Results The level of cell mortality, CD11b expression and the oxidative burst capacity were elevated in NET-treated neutrophils. Also, the elevation caused by the SLE NET was higher than that produced by the healthy NET. Conclusion The decreased neutrophil viability was not due to the increase in apoptosis; rather, it was because of the augmentation of other inflammatory cell-death modes. The upregulation of CD11b implies that NET causes neutrophils to more actively contribute to inflammation. The increased oxidative burst capacity of neutrophils can play a double role in inflammation. Overall, the effects induced by NET on neutrophils help prolong inflammation; accordingly, the NET collected from SLE patients is stronger than the NET from healthy individuals.

Assessment of blood neutrophil oxidative burst activity in dairy cows during the period of parturition

1998 ◽  
Vol 67 (3) ◽  
pp. 421-426 ◽  
Author(s):  
F. Moreira da Silva ◽  
C. Burvenich ◽  
A. M. Massart Leën ◽  
L. Brossé
Keyword(s):  
Oxidative Burst ◽  
Normal Values ◽  
Leukocyte Count ◽  
Post Partum ◽  
Burst Activity ◽  
Total Leukocyte Count ◽  
Blood Neutrophil ◽  
Oxidative Burst Activity ◽  
Peripartum Period ◽  
Neutrophil Oxidative Burst

AbstractBovine blood neutrophil oxidative burst activity, the total leukocyte count and its differentiation, as well as progesterone, glucose and cortisol levels were studied on 10 cows from 1 week prior to calving until 8 weeks after parturition.From day –7 before parturition a decrease in the metabolic oxidative burst activity of neutrophils was observed, reaching the lowest value 3 days after calving (0·28 (s.e. 0·06) nmol/l H2O2per min 106 neutrophils) (P<0·01). Thereafter, values increased with a maximum of activity 26 days after parturition (0·57 (s.e. 0·05) nmol/H202 per min per 106 neutrophils). At the end of the experiment the neutrophils H2O2-productionwas comparable to values observed on day–7.The maximum total leukocyte count was observed on the day of parturition (8, 108 (s.e. 370) leukocytes per /A blood) (P<0·01) returning to normal levels about 3 weeks after calving. Lymphocytes and neutrophils showed a trend analogous to that of the total leukocytes. Eosinophils and monocytes did not change during the entire period. With the rapid increase of circulating neutrophils observed on the calving day, a marked left shift was observed with a maximum number of immature neutrophils (48·6%) observed on day +3 (1477 (s.e. 242) immature neutrophils per fjl blood) (P<0·01), reaching normal values about 4 weeks after parturition.A significant increase (F < 0·01) of plasma glucose was found on the day of parturition (91·4 (s.e. 6·8) mg per 100 ml plasma), while the levels of cortisol reached their maximum 3 days after calving (22·05 (s.e. 8·46) nglml plasma).Progesterone levels averaged 4·07 (s.e. 1·21) jigll 7 days before calving, declining precipitously to 0·06 (s.e. 0·45) Hg/l 3 days after calving (F < 0·01), remaining very low for a period of about 3 weeks. Minimum and maximum anoestrus post-partum were 22 and 57 days, respectively.Despite great hormonal variation observed in the peripartum period, no significant correlation was found between measured hormones and the oxidative burst activity of neutrophils.

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