Assessment of HOCl Production in Human blood: Experimental Optimization and Proof of Concept for the Antioxidant Activity of Flavonoids in this Complex Matrix

Elderberry (Sambucus nigra L.) is a widely disseminated plant that produces bright black berries containing high quantities of anthocyanins, acknowledged for their bioactivity and dye capacity. Besides other applications, anthocyanins might be employed as natural colouring agents to reduce/eliminate the use of artificial dyes, while providing positive effects on consumers’ health. Herein, the anthocyanins profile of elderberry juice was characterised by HPLC-DAD-ESI/MSn. In addition, its antioxidant capacity and cytotoxicity were also evaluated. As a proof-of-concept of its colouring capacity, elderberry juice was added (different percentages) to a highly appreciated and consumed pastry product (croissant) and compared with a commercial dye (black carrot). In general, the nutritional properties of control and coloured croissants were similar, despite some individual differences in sugars and fatty acids. In turn, the appearance obtained with elderberry juice incorporation might be considered innovative, besides partially maintaining the anthocyanins content of fresh juice and showing considerable antioxidant activity.

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Spatial Meta-transcriptomes of human and murine intestines

10.1101/2021.12.13.472336 ◽

2021 ◽

Author(s):

Lin Lyu ◽

Ru Feng ◽

Xue Li ◽

Xiaofei Yu ◽

GuoQiang Chen ◽

...

Keyword(s):

Simultaneous Analysis ◽

Transcriptomic Analysis ◽

Microbial Abundance ◽

Proof Of Concept ◽

Analysis Pipeline ◽

Experimental Optimization ◽

Transcriptomic Data ◽

Microbial Distribution ◽

The Way

We developed an analysis pipeline that can extract microbial sequences from Spatial Transcriptomic data and assign taxonomic labels to them, generating a spatial microbial abundance matrix in addition to the default host expression one, enabling simultaneous analysis of host expression and microbial distribution. We applied it on both human and murine intestinal datasets and validated the spatial microbial abundance information with alternative assays. Finally, we present a few biological insights that can be gained from this novel data. In summary, this proof of concept work demonstrated the feasibility of Spatial Meta-transcriptomic analysis, and pave the way for future experimental optimization.

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A Green Antioxidant Activity-Integrated Dual-Standard Method for Rapid Evaluation of the Quality of Traditional Chinese Medicine Xuebijing Injection by On-Line DPPH-CE-DAD

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/2712476 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 3

Author(s):

Jin Li ◽

Jiao Liu ◽

Wei Liu ◽

Xie-an Yu ◽

Jun Cao ◽

...

Keyword(s):

Antioxidant Activity ◽

Quality Control ◽

Antioxidant Properties ◽

Array Detector ◽

Complex Matrix ◽

Xuebijing Injection ◽

Total Antioxidant ◽

On Line ◽

Green Technique

Much attention has been focused on treatment of sepsis which leads to high mortality all over the world in every year. Antioxidant activity seems to play a prominent role in the treatment of sepsis exhibited by Xuebijing injection. The aim of the present research was to develop an on-line 1, 1-diphenyl-2-picrylhydrazyl- (DPPH-) capillary electrophoresis-diode array detector (on-line DPPH-CE-DAD) method for rapidly assessing antioxidant properties and efficacious material basis of antioxidant activity as a way of quality control of Xuebijing injection. Several parameters affecting the separation were investigated, including the pH and concentrations of buffer, SDS,β-CD, and organic modifier as well as voltage and cassette temperature. Compared to previous traditional method, this improved method shortened the experimental cycle and became more efficient because it was successfully applied to analyze total antioxidant activity and contents of twelve antioxidants of Xuebijing injection under the same condition. The results revealed that the on-line DPPH-CE-DAD method was a reagent-saving, rapid, feasible, and green technique for quality control of Xuebijing injection in terms of pharmacological activity and contents of active ingredients. It also offered new opportunities for the analysis of antioxidant activity of complex matrix.

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Inside Cover: Optimization of the Antioxidant Activity of Hydroxy-Substituted 4-Thiaflavanes: A Proof-of-Concept Study (Chem. Eur. J. 44/2011)

Chemistry - A European Journal ◽

10.1002/chem.201190216 ◽

2011 ◽

Vol 17 (44) ◽

pp. 12214-12214

Author(s):

Caterina Viglianisi ◽

Maria Grazia Bartolozzi ◽

Gian Franco Pedulli ◽

Riccardo Amorati ◽

Stefano Menichetti

Keyword(s):

Antioxidant Activity ◽

Proof Of Concept ◽

Concept Study

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Attenuation of Oxidative Stress by Cannabinoids and Cannabis Extracts in Differentiated Neuronal Cells

Pharmaceuticals ◽

10.3390/ph13110328 ◽

2020 ◽

Vol 13 (11) ◽

pp. 328

Author(s):

Aruna Raja ◽

Soha Ahmadi ◽

Fernanda de Costa ◽

Nan Li ◽

Kagan Kerman

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Neuronal Cells ◽

In Vitro Models ◽

Proof Of Concept ◽

In Vitro System ◽

Pathological Conditions ◽

Stress Therapy ◽

Antagonist Effect

In this proof-of-concept study, the antioxidant activity of phytocannabinoids, namely cannabidiol (CBD) and Δ9- tetrahydrocannabinol (THC), were investigated using an in vitro system of differentiated human neuronal SY-SH5Y cells. The oxidative stress was induced by hydrogen peroxide, as reactive oxygen species (ROS). Alzheimer’s disease (AD)-like pathological conditions were mimicked in vitro by treating the differentiated neuronal cells with amyloid-β1–42 (Aβ1–42) in the presence of Cu(II). We showed that THC had a high potency to combat oxidative stress in both in vitro models, while CBD did not show a remarkable antioxidant activity. The cannabis extracts also exhibited a significant antioxidant activity, which depended on the ratio of the THC and CBD. However, our results did not suggest any antagonist effect of the CBD on the antioxidant activity of THC. The effect of cannabis extracts on the cell viability of differentiated human neuronal SY-SH5Y cells was also investigated, which emphasized the differences between the bioactivity of cannabis extracts due to their composition. Our preliminary results demonstrated that cannabis extracts and phytocannabinoids have a promising potential as antioxidants, which can be further investigated to develop novel pharmaceuticals targeting oxidative stress therapy.

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