Complement consumption by Photobacterium damselae subsp. piscicida in seabream, red porgy and seabass normal and immune serum. Effect of the capsule on the bactericidal effect

2006 ◽  
Vol 20 (5) ◽  
pp. 709-717 ◽  
Author(s):  
F ACOSTA ◽  
A ELLIS ◽  
J VIVAS ◽  
D PADILLA ◽  
B ACOSTA ◽  
...  
Keyword(s):  
Bactericidal Effect ◽  
Immune Serum ◽  
Red Porgy ◽  
Photobacterium Damselae ◽  
Serum Effect

scholarly journals In Silico Toxicological, Anti-Tubercular Effect Evaluation And In Vitro Marine Pathogenic Bacteria Inhibition of N-[(3-Chloro-4-Nitro-Phenyl)Methyleneamino]Pyridine-4-Carboxamidine

2019 ◽  
Vol 9 (6) ◽  
pp. 23-27
Author(s):  
Kamel Mokhnache ◽  
EL-Khamsa Soltani ◽  
Soraya Madoui ◽  
Hanane Khither ◽  
Ahlem Karbab ◽  
...  
Keyword(s):  
In Silico ◽  
Pathogenic Bacteria ◽  
Vibrio Anguillarum ◽  
Subcutaneous Administration ◽  
Covalent Bond ◽  
Metabolic Activation ◽  
Bactericidal Effect ◽  
Effect Evaluation ◽  
Photobacterium Damselae

The hydrazone; N-[(3-chloro-4-nitro-phenyl) methyleneamino] pyridine-4-carboxamidine (H) was selected for in silico toxicological and in vitro bactericidal studies. Toxicological investigation was carried out using software program, such as eMolTox and Gusar, for the toxic substructure determination, and acute rat toxicity prediction respectively. In vitro bactericidal effect evaluation was investigated using tow marine pathogenic bacteria; Vibrio anguillarum and Photobacterium damselae. Computational results determinate toxicophores of (H), which are nitro-aromatic part, hydrazine group, and quaternary carbon, were predicted as responsible for Idiosyncratic toxicity metabolic activation, covalent bond with DNA, and hepatotoxicity respectively. In addition, the predicted LD50 of (H) are 1086, 244, 1816, and 823.40 mg/kg in intraperitenial, intravenous, oral and subcutaneous administration respectively. For bactericidal results, H exhibited an excellent effect with inhibition percentages of 98.65 and 98.83% at the concentrations of 1000 and 500 µg/mL against Vibrio anguillarum respectively, the same effect was demonstrated against Photobacterium damselae with inhibition percentages of 97.74 and 97.98 % at the same concentrations. For anti-tubercular effect prediction, results revealed that H has an excellent effect with probability percentage of 84.6%.   Keyword: Hydrazone, toxicophore, LD50, Anti-tubercular, Vibrio anguillarum, Photobacterium damselae.     

scholarly journals BACTERICIDAL EFFECT OF HUMAN SERUM ON BORRELIA MIYAMOTOI, CAUSATIVE AGENT OF IXODES TICK-BORNE BORRELIOSIS (ITBB-BM)

2018 ◽  
pp. 58-67 ◽  
Author(s):  
A. E. Platonov ◽  
J. .. Koetsveld ◽  
O. A. Stukolova ◽  
A. S. Dolgova ◽  
N. M. Kolyasnikova ◽  
...  
Keyword(s):  
Human Serum ◽  
Protein Microarray ◽  
Bactericidal Effect ◽  
Dark Field ◽  
Immune Serum ◽  
Heat Inactivation ◽  
Borrelia Miyamotoi ◽  
Serum Samples ◽  
Variable Major Proteins

Aim. Our aim was to study the bactericidal effect of human serum on Borrelia miyamotoi in vitro. Materials and methods. B. miyamotoi spirochetes (strains HT31 and LB-2001) were incubated in non-immune serum of healthy donors (SHD) and in heat inactivated complement-depleted SHD, as well as in serum samples of the patients recovered from ITBB-BM. The viability, that is motility, of borrelia after incubation was investigated by dark-field microscopy. The levels ofserum antibody to B.miyamofoi-specificproteins (GlpQ enzyme and four variable major proteins Vlpl5/16, Vlpl8, Vspl, and Vlp5) were measured by specially designed plane protein microarray. Results. Borrelia fully retain their viability in non-immune SHD, but their motility is partially or completely suppressed by the addition of serum from ITBB-BM convalescents or rabbit antibodies to Д. miyamotoi. The immobilizing effect of the immune serum is substantially inhibited by its heat-inactivation, which indicates that immobilizing effect is mediated by the complement system. Conclusion. Antibody-dependent complement-mediated bactericidal action ofhuman blood serum is probably not the only and 100% effective mechanism for human defense against B. miyamotoi infection, but requires support from cellular immunity.

The serological activities of immunoconglutinin

1969 ◽  
Vol 15 (9) ◽  
pp. 981-987
Author(s):  
D. G. Ingram
Keyword(s):  
Guinea Pig ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Bactericidal Effect ◽  
Immune Serum ◽  
Antigenic Stimulation ◽  
Sheep Red Blood Cells

Immunoconglutinin is an antibody against fixed complement which appears in the serum of an animal after a strong antigenic stimulation. Using alexinated sheep red blood cells as antigen, various reactions between rabbit immunoconglutinin and complement were examined. It was shown that immunoconglutinin acted as an antibody to bring about the binding of horse, rabbit, and guinea pig complement. In the presence of hemolytic complement, immunoconglutinin had hemolysin activity.Immunoconglutinin was specifically absorbed from immune serum by using the antibody and complement present in the serum, thus demonstrating that immunoconglutinin can react with autologous complement.Rabbit sera containing heterostimulated immunoconglutinin showed an increased and prolonged bactericidal effect. It is suggested that the function of immunoconglutinin is to enhance the normal activities of complement.

Bactericidal effect of bovine normal and immune serum, colostrum and milk againstHelicobacter pylori

1995 ◽  
Vol 78 (6) ◽  
pp. 655-662 ◽  
Author(s):  
H. Korhonen ◽  
E.-L. Syväoja ◽  
H. Ahola-Luttila ◽  
S. Sivelä ◽  
S. Kopola ◽  
...  
Keyword(s):  
Bactericidal Effect ◽  
Immune Serum

scholarly journals The function of the Vi antigen of Salmonella paratyphi A. Comparison between virulence, agglutinability by anti-O immune sera and sensitivity to the bactericidal action in vitro

1957 ◽  
Vol 55 (1) ◽  
pp. 91-101 ◽  
Author(s):  
A. L. Olitzki ◽  
E. Fleischhacker ◽  
Z. Olitzki
Keyword(s):  
Bactericidal Effect ◽  
Immune Serum ◽  
Bactericidal Action ◽  
Virulent Strains ◽  
Additional Antigen ◽  
Vi Antigen

Experiments were performed which show that virulent strains of Salmonella paratyphi A contain an additional antigen which has all the properties of a Vi antigen.The anti-Vi immune serum combined in suitable proportions with an anti-0 immune serum exerted maximal bactericidal effect on virulent strains.

Actin and α-tubulin immuno-EM labelings reveal differences in intra versus interphotoreceptor matrix

1990 ◽  
Vol 48 (3) ◽  
pp. 466-467
Author(s):  
Matti Järvilehto ◽  
Riitta Harjula
Keyword(s):  
Compound Eye ◽  
Frozen Section ◽  
Smooth Muscle Actin ◽  
Photoreceptor Cells ◽  
Immune Serum ◽  
Cell Organelles ◽  
Calliphora Erythrocephala ◽  
Optical Axes ◽  
Interphotoreceptor Matrix ◽  
Similar Kind

The photoreceptor cells in the compound eyes of higher diptera are clustered in groups (ommatidia) of eight receptor cells. The cells from six adjacent ommatidia are organized into optical units, neuro-ommatia sharing the same visual field. In those ommatidia the optical axes of the photopigment containing structures (rhabdomeres) are parallel. The rhabdomeres of the photoreceptor cells are separated from each other by an interstitial i.e innerommatidial space (IOS). In the photoreceptor cell body, besides of the normal cell organelles, a cellular matrix is a structurally apparent component. Similar kind of reticular formation is also found in the IOS containing some unidentified filamentary substance, of which composition and functional significance for optical properties of vision is the aim of this report.The prefixed (2% PA + 0.2% GA in 0.1-n phosphate buffer, pH 7.4, for 1h), frozen section blocks of the compound eye of the blowfly (Calliphora erythrocephala) were prepared by immuno-cryo-techniques. The ultrathin cryosections were incubated with antibodies of monoclonal α-tubulin and polyclonal smooth muscle actin. Control labelings of excess of antigen, non-immune serum and non-present antibody were perforated.

Properties of Washed Human Platelets

1977 ◽  
Vol 37 (02) ◽  
pp. 291-308 ◽  
Author(s):  
Raelene L Kinlough-Rathbone ◽  
J Fraser Mustard ◽  
Marian A Packham ◽  
Dennis W Perry ◽  
Hans-Joachim Reimers ◽  
...  
Keyword(s):  
Major Effect ◽  
Human Platelets ◽  
Immune Serum ◽  
Immune Serum Globulin ◽  
Serum Globulin ◽  
Platelet Adherence ◽  
Low Concentrations ◽  
Induced Aggregation ◽  
Extensive Release ◽  
Protein Free Medium

SummaryWe have shown previously that washed human platelets resuspended in Tyrode solution containing albumin and apyrase maintain their disc shape and their ability to aggregate upon the addition of low concentrations of ADP, providing fibrinogen is added to the suspending medium. We have now examined their responses to other aggregating and release-inducing agents. Collagen, arachidonate, thrombin, immune serum globulin, the ionophore A 23, 187 and phytohaemagglutinin from Phaseolus vulgaris caused aggregation and release of granule contents. The response to adrenaline was variable. Serotonin caused the platelets to change shape but no aggregation or release occurred. Addition of a small amount of plasma was necessary for ristocetin-induced aggregation. Polylysine caused immediate platelet-to-platelet adherence with little or no release of granule contents. Responses to collagen or thrombin were greater in a modified medium containing magnesium but no calcium; in this medium, aggregation caused by ADP or polylysine was followed by the release of granule contents whereas these agents caused aggregation without release in a medium with both calcium and magnesium. When protein was omitted from the suspending medium, platelet aggregation in response to ADP was variable. In this medium, collagen and thrombin caused more extensive release than in the albumin-containing medium. Aggregation by polylysine was accompanied by release and extensive lysis in the protein-free medium. Thus, the composition of the final resuspending medium has a major effect on the responses of washed human platelets to aggregating agents.

A Study of the Efficiency of Modern Domestic Disinfectants in the System of TB Control Activities

2015 ◽  
Vol 2 (2) ◽  
pp. 26-31 ◽  
Author(s):  
A. Paliy ◽  
A. Zavgorodniy ◽  
B. Stegniy ◽  
A. Gerilovych
Keyword(s):  
Molecular Genetic ◽  
Critical Role ◽  
Bactericidal Effect ◽  
Chain Reaction ◽  
Tb Control ◽  
Source Of Infection ◽  
Polymerase Chain ◽  
And Control ◽  
Chemical Groups ◽  
Test Objects

Due to the absence of elaborated effi cient means for specifi c prevention of bovine tuberculosis, it is ex- tremely important to detect and eliminate the source of infection and to take veterinary and sanitary preven- tive measures. Here the critical role is attributed to disinfection, which breaks the epizootic chain due to the elimination of pathogenic microorganisms in the environment and involves the application of disinfectants of different chemical groups. Aim. To study the tuberculocidal properties of new disinfectants DZPT-2 and FAG against atypical mycobacteria Mycobacterium fortitum and a TB agent Mycobacterium bovis. Methods. The bacteriological and molecular-genetic methods were used. Results. It was determined that DZPT-2 prepara- tion has bactericidal effect on M. fortuitum when used in the concentration of 2.0 % of the active ingredient (AI) when exposed for 5–24 h, while disinfectant FAG has a bactericidal effect in the concentration of 2.0 % when exposed for 24 h. Disinfectant DZPT-2 in the concentration of 2.0 % of the AI, when exposed for 5–24 h, and FAG preparation in the concentration of 2.0 %, when exposed for 24 h, and with the norm of consump- tion rate of 1 cubic decimeter per 1 square meter disinfect the test-objects (batiste, wood, glazed tile, metal, glass), contaminated with the TB agent M. bovis. Conclusions. Disinfecting preparations of DZPT-2 in the concentration of 2.0 % of AI when exposed for 5 h and FAG in the concentration of 2.0 % when exposed for 24 h may be used in the complex of veterinary and sanitary measures to prevent and control TB of farm ani- mals. The possibility of using the polymerase chain reaction as an additional method of estimating tuberculo- cide activity of disinfectants was proven.

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