3-Hydroxy-3-methylglutaryl coenzyme A reductase 1 (HMG1) is highly associated with the cell division during the early stage of fruit development which determines the final fruit size in Litchi chinensis

Gene ◽  
2012 ◽  
Vol 498 (1) ◽  
pp. 28-35 ◽  
Author(s):  
Xia Rui ◽  
Li Caiqin ◽  
Lu Wangjin ◽  
Du Juan ◽  
Wang Zehuai ◽  
...  
Keyword(s):  
Cell Division ◽  
Fruit Development ◽  
Coenzyme A ◽  
Early Stage ◽  
Fruit Size ◽  
Litchi Chinensis ◽  
Coenzyme A Reductase

scholarly journals Comparative Analysis of Transcriptomes to Identify Genes Associated with Fruit Size in the Early Stage of Fruit Development in Pyrus pyrifolia

2018 ◽  
Vol 19 (8) ◽  
pp. 2342 ◽  
Author(s):  
Shuang Jiang ◽  
Haishan An ◽  
Jun Luo ◽  
Xiaoqing Wang ◽  
Chunhui Shi ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Cell Division ◽  
Fruit Development ◽  
Early Stage ◽  
Fruit Size ◽  
Pyrus Pyrifolia ◽  
Small Fruit ◽  
Short Period ◽  
The Difference ◽  
Cytokinin Dehydrogenase

Pear (Pyrus L.) is an important commercial fruit in the world. The fruit size is one of the important characters in fruit quality. The previous research reported that the fruit size of pear was mainly caused by the number of cell in about 40 days after blossom (DAB) in nature. However, studies about the mechanisms underlying cell division in young fruit development are very limited in pear. Two pear accessions codenamed ‘GH59B’ with big fruit and ‘GH81S’ with small fruit in three stages were sampled and the RNA-seq high-throughput sequencing was used to evaluate changes of gene transcription levels in the early stage of fruit development. The difference of cell size among two samples was little in 40 DAB, implying that the difference of the fruit size was caused by the number of the cell. More than 274,517,982 high quality reads from six libraries of fruit development were sequenced. A total of 797 differentially expressed genes (DEGs) were identified. Three cytokinin dehydrogenase genes and two gibberellin 2-beta-dioxygenase gene were identified in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to zeatin and gibberellin. Their expression was upregulated at 20 DAB in ‘GH81S’ and at 30 DAB in ‘GH59B’, suggesting that the small fruit size might be related to the early degradation of cytokinin and gibberellin inducing a short period of cell division. A total of 38 DEGs of transcription factors were found and 23 DEGs including NAC, ERF and bHLH transcription factors were highly related with cytokinin dehydrogenase and gibberellin dioxygenase genes. Altogether, the results of the present study provide information from a comprehensive gene expression analysis and insight into the molecular mechanism underlying the difference of fruit size in Pyrus pyrifolia.

scholarly journals Transformation of Tomato with the Melon 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Leads to Increase of Fruit Size

2003 ◽  
Vol 20 (4) ◽  
pp. 297-303 ◽  
Author(s):  
Toshihiro KOBAYASHI ◽  
Sumie KATO-EMORI ◽  
Keno TOMITA ◽  
Hiroshi EZURA
Keyword(s):  
Coenzyme A ◽  
Fruit Size ◽  
Coenzyme A Reductase

scholarly journals Regulation of Fruit Growth and Fruit Size in Apple

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1097A-1097
Author(s):  
Anish Malladi ◽  
Peter Goldsbrough ◽  
Peter Hirst
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Cell Division ◽  
Fruit Development ◽  
Fruit Size ◽  
Cell Number ◽  
Rt Pcr ◽  
Cell Production ◽  
Golden Delicious ◽  
Dividing Cells

Fruit development in apple cultivars varying in their ultimate fruit size was analyzed using cytology, flow cytometry (FCM), and semi-quantitative RT-PCR. Fruit size variation across cultivars was largely explained by variation in cell number. The cell division phase lasted for less than 30 days in all varieties, less than previously believed. A distinct overlap between the cell division and cell expansion phases was present. Analysis of the relative cell production rate (rCPR) showed a major peak about 10 days after full bloom (DAFB) after which it declined. Comparison of the rCPR across varieties suggested distinct patterns of cell production with `Gala' having a low but sustained rCPR, `Pixy Crunch' a short but high rCPR, and `Golden Delicious' having a high and sustained rCPR. FCM analysis also showed similar patterns with a peak in the proportion of dividing cells about 10 DAFB followed by a decline. To further understand regulation of cell number, four cell cycle related genes were cloned from `Gala'. Cyclin Dependent Kinase B (CDK B) and Cyclin B were found to be highly cell division phase specific in their expression. Analysis of gene expression by semi-quantitative RT-PCR indicated peak expression of these two genes at 5-10 DAFB, consistent with the peaks in rCPR and proportion of dividing cells. Comparison of gene expression across the varieties showed higher peak expression of the above genes in the larger-fruited `Golden Delicious' than in the smaller-fruited `Gala.' This study provides novel insight into the regulation of fruit development in apple and also suggests a role for the cell cycle genes in fruit size regulation.

scholarly journals The Potential Role of Auxin and Abscisic Acid Balance and FtARF2 in the Final Size Determination of Tartary Buckwheat Fruit

2018 ◽  
Vol 19 (9) ◽  
pp. 2755 ◽  
Author(s):  
Moyang Liu ◽  
Zhaotang Ma ◽  
Tianrun Zheng ◽  
Jing Wang ◽  
Li Huang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Cell Division ◽  
Fruit Development ◽  
Fruit Size ◽  
Tartary Buckwheat ◽  
Final Size ◽  
Division Rate ◽  
Cell Division Rate ◽  
The Difference ◽  
Number Of Cells

Tartary buckwheat is a type of cultivated medicinal and edible crop with good economic and nutritional value. Knowledge of the final fruit size of buckwheat is critical to its yield increase. In this study, the fruit development of two species of Tartary buckwheat in the Polygonaceae was analyzed. During fruit development, the size/weight, the contents of auxin (AUX)/abscisic acid (ABA), the number of cells, and the changes of embryo were measured and observed; and the two fruit materials were compared to determine the related mechanisms that affected fruit size and the potential factors that regulated the final fruit size. The early events during embryogenesis greatly influenced the final fruit size, and the difference in fruit growth was primarily due to the difference in the number of cells, implicating the effect of cell division rate. Based on our observations and recent reports, the balance of AUX and ABA might be the key factor that regulated the cell division rate. They induced the response of auxin response factor 2 (FtARF2) and downstream small auxin upstream RNA (FtSAURs) through hormone signaling pathway to regulate the fruit size of Tartary buckwheat. Further, through the induction of fruit expansion by exogenous auxin, FtARF2b was significantly downregulated. The FtARF2b is a potential target for molecular breeding or gene editing.

scholarly journals 682 New Perspectives on the Influence of Mid-season Environment on Apple Fruit Characteristics

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 516C-516
Author(s):  
D.S. Tustin ◽  
T. Fulton ◽  
H. Brown
Keyword(s):  
Cell Division ◽  
Fruit Development ◽  
Climatic Variability ◽  
Fruit Size ◽  
Cortical Cell ◽  
Dry Weight ◽  
Apple Fruit ◽  
Major Influence ◽  
Controlled Environment ◽  
Crop Load

Growth of apple fruit can be described as an initial exponential phase lasting the 40+ days of fruit cell division followed by a more-or-less linear phase where growth is by cell expansion. Temperature is a major influence on fruit growth rate during the cell division phase, thereby affecting fruit size at maturity. However it is generally thought that temperature has less-direct impact on fruit development during the fruit expansion phase. Our observations of apple growth among regions and seasons of considerable climatic variability led us to speculate that temperature may impact directly on fruit development during fruit expansion but that responses may be interactive with carbon balance (crop load) influences. Controlled environment studies are being used to examine this hypothesis. Potted `Royal Gala' trees set to three levels of crop (one fruit per 250, 500, or 1000 cm2 leaf area) were grown from 56 to 112 DAFB in day/night temperature regimes of 18/6, 24/12, and 30/18 °C. All trees grew in field conditions prior to and following the controlled environment treatments. Treatments were harvested when 20% to 25% of fruit on trees showed the visual indicators used commercially to indicate harvest maturity. Fruit were evaluated using attributes that determine quality and that may have implications for fruit post harvest behaviour. Temperature and crop load influences on time to maturity, fruit fresh and dry weight, fruit DM content, fruit firmness, fruit airspace content and estimated fruit cortical cell size will be presented and implications discussed.

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