Cucumis sativus L-type lectin receptor kinase (CsLecRK) gene family response to Phytophthora melonis, Phytophthora capsici and water immersion in disease resistant and susceptible cucumber cultivars

Gene ◽  
2014 ◽  
Vol 549 (2) ◽  
pp. 214-222 ◽  
Author(s):  
Tingquan Wu ◽  
Rui Wang ◽  
Xiaomei Xu ◽  
Xiaoming He ◽  
Baojuan Sun ◽  
...  
Keyword(s):  
Gene Family ◽  
Cucumis Sativus ◽  
Water Immersion ◽  
Phytophthora Capsici ◽  
Receptor Kinase ◽  
Cucumis Sativus L ◽  
Lectin Receptor ◽  
Disease Resistant ◽  
Phytophthora Melonis

scholarly journals Genome-Wide Characterization of GRAS Family and Their Potential Roles in Cold Tolerance of Cucumber (Cucumis sativus L.)

2020 ◽  
Vol 21 (11) ◽  
pp. 3857 ◽  
Author(s):  
Xiaohong Lu ◽  
Wenqian Liu ◽  
Chenggang Xiang ◽  
Xiaojun Li ◽  
Qing Wang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Gene Family ◽  
Cold Tolerance ◽  
Cucumis Sativus ◽  
Expression Patterns ◽  
Cold Treatment ◽  
Evolutionary Tree ◽  
Cucumis Sativus L ◽  
Gras Family ◽  
Biological Stress

Cucumber (Cucumis sativus L.) is one of the most important cucurbit vegetables but is often subjected to stress during cultivation. GRAS (gibberellic acid insensitive, repressor of GAI, and scarecrow) genes encode a family of transcriptional factors that regulate plant growth and development. In the model plant Arabidopsis thaliana, GRAS family genes function in formation of axillary meristem and root radial structure, phytohormone (gibberellin) signal transduction, light signal transduction and abiotic/biological stress. In this study, a gene family was comprehensively analyzed from the aspects of evolutionary tree, gene structure, chromosome location, evolutionary and expression pattern by means of bioinformatics; 37 GRAS gene family members have been screened from cucumber. We reconstructed an evolutionary tree based on multiple sequence alignment of the typical GRAS domain and conserved motif sequences with those of other species (A. thaliana and Solanum lycopersicum). Cucumber GRAS family was divided into 10 groups according to the classification of Arabidopsis and tomato genes. We conclude that tandem and segmental duplication have played important roles in the expansion and evolution of the cucumber GRAS (CsaGRAS) family. Expression patterns of CsaGRAS genes in different tissues and under cold treatment, combined with gene ontology annotation and interaction network analysis, revealed potentially different functions for CsaGRAS genes in response to cold tolerance, with members of the SHR, SCR and DELLA subfamilies likely playing important roles. In conclusion, this study provides valuable information and candidate genes for improving cucumber tolerance to cold stress.

scholarly journals Genome-wide identification and characterization of lectin receptor-like kinase gene family in cucumber and expression profiling analysis under different treatments

2020 ◽  
Author(s):  
Duo Lv ◽  
Gang Wang ◽  
Yue Chen ◽  
Liang-Rong Xiong ◽  
Jing-Xian Sun ◽  
...  
Keyword(s):  
Gene Family ◽  
Stress Responses ◽  
Expression Patterns ◽  
Regulatory Elements ◽  
Kinase Gene ◽  
Cucumis Sativus L ◽  
Lectin Receptor ◽  
Genome Wide ◽  
Cucumber Genome ◽  
And Function

Abstract Background Lectin receptor-like kinases (LecRLKs) are a class of membrane proteins found in plants that are involved in diverse functions, including plant development and stress responses. Although LecRLK families have been identified in a variety of plants, a comprehensive analysis has not yet been undertaken in cucumber ( Cucumis sativus L.).Results In this study, 46 putative LecRLK genes were identified in cucumber genome, including 23 G-type, 22 L-type and 1 C-type LecRLK genes. They unequally distributed on all 7 chromosomes with a clustering trendency. Most of the genes in the cucumber LecRLK (Cs LecRLK) gene family lacked introns. In addition, there were many regulatory elements associated with phytohormone and stress on these genes’ promoters. Transcriptome data demonstrated that distinct expression patterns of CsLecRLK genes in various tissues. Furthermore, we found that each member of the CsLecRLK family had its own unique expression pattern under hormone and stress treatment by the quantitative real time PCR (qRT-PCR) analysis.Conclusion This study provides a better understanding of the evolution and function of LecRLK gene family in cucumber, and opens the possibility to explore the roles that LecRLK s might play in the life cycle of cucumber.

scholarly journals Expression analysis of defense-related genes in cucumber (Cucumis sativus L.) against Phytophthora melonis

2020 ◽  
Vol 47 (7) ◽  
pp. 4933-4944 ◽  
Author(s):  
Lida Hashemi ◽  
Ahmad Reza Golparvar ◽  
Mehdi Nasr-Esfahani ◽  
Maryam Golabadi
Keyword(s):  
Cucumis Sativus ◽  
Expression Analysis ◽  
Cucumis Sativus L ◽  
Phytophthora Melonis

scholarly journals Genome-wide identification and expression analysis of CYP450 gene family in Cucumis sativus L.

2021 ◽  
Author(s):  
hongyu wang ◽  
Pengfei Li ◽  
Yu Wang ◽  
Chunyu Chi ◽  
Guohua Ding
Keyword(s):  
Subcellular Localization ◽  
Gene Family ◽  
Cucumis Sativus ◽  
Expression Profile ◽  
Evolutionary Relationship ◽  
Evolutionary Analysis ◽  
Cucumis Sativus L ◽  
P450 Gene ◽  
Genome Wide ◽  
A Genome

Abstract The cytochrome P450 (CYP450) gene family plays a vital role in basic metabolism and enhances plant resistance to stress and pests. However, little information is available on the genome-wide characterization and evolutionary relationship of the CYP450 gene family in Cucumis sativus L. In the present study, a genome-wide bioinformatics analysis was performed, including gene structure, conserved motif, cis-acting promoter element, evolutionary analysis, collinearity, subcellular localization, and expression profile. The gene expression profile of CYP450 was verified using transcriptome sequencing and quantitative reverse transcription polymerase chain reaction. A total of 165 P450 genes were identified in the cucumber genome. These genes were classified into eight subfamilies and unevenly distributed on seven chromosomes. Subcellular localization predicted that most of P450 genes were located in chloroplasts and a few were located on the plasma membrane. CYP450 genes were differentially expressed in different tissues and in response to salicylic acid (SA) treatment. The sizes of all cucumber P450 proteins ranged from 317 to 1,056 aa, the theoretical isoelectric points ranged from 5.05 to 10.31, and the molecular weights ranged from 36,095 to 121,403 KD. This study provides a theoretical basis for further research on the biological functions of the P450 gene in cucumber plants.

RNA expression, protein activity, and interactions in the ACC synthase gene family in cucumber (Cucumis sativus L.)

2018 ◽  
Vol 59 (1) ◽  
pp. 81-91 ◽  
Author(s):  
Jeong Hwan Lee ◽  
Young-Cheon Kim ◽  
Daeun Choi ◽  
Ji Hoon Han ◽  
Youjin Jung ◽  
...  
Keyword(s):  
Gene Family ◽  
Cucumis Sativus ◽  
Acc Synthase ◽  
Rna Expression ◽  
Protein Activity ◽  
Cucumis Sativus L

scholarly journals Genome-Wide Identification and Characterization of Lectin Receptor-Like Kinase Gene Family in Cucumber and Expression Profiling Analysis under Different Treatments

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 1032 ◽  
Author(s):  
Duo Lv ◽  
Gang Wang ◽  
Liang-Rong Xiong ◽  
Jing-Xian Sun ◽  
Yue Chen ◽  
...  
Keyword(s):  
Gene Family ◽  
Stress Responses ◽  
Expression Patterns ◽  
Regulatory Elements ◽  
Pcr Analysis ◽  
Kinase Gene ◽  
Cucumis Sativus L ◽  
Lectin Receptor ◽  
Genome Wide ◽  
And Function

Lectin receptor-like kinases (LecRLKs) are a class of membrane proteins found in plants that are involved in diverse functions, including plant development and stress responses. Although LecRLK families have been identified in a variety of plants, a comprehensive analysis has not yet been undertaken in cucumber (Cucumis sativus L.). In this study, 46 putative LecRLK genes were identified in the cucumber genome, including 23 G-type and 22 L-type, and one C-type LecRLK gene. They were unequally distributed on all seven chromosomes, with a clustering tendency. Most of the genes in the cucumber LecRLK (CsLecRLK) gene family lacked introns. In addition, there were many regulatory elements associated with phytohormones and stress on these genes’ promoters. Transcriptome data demonstrated distinct expression patterns of CsLecRLK genes in various tissues. Furthermore, we found that each member of the CsLecRLK family had its own unique expression pattern under hormone and stress treatment by the quantitative real-time PCR (qRT-PCR) analysis. This study provides a better understanding of the character and function of the LecRLK gene family in cucumber and opens up the possibility to exploring the roles that LecRLKs might play in the life cycle of cucumber.

The expression patterns of Cucumis sativus WRKY (CsWRKY) family under the condition of inoculation with Phytophthora melonis in disease resistant and susceptible cucumber cultivars

2015 ◽  
Vol 95 (6) ◽  
pp. 1121-1131 ◽  
Author(s):  
Xiaomei Xu ◽  
Rui Wang ◽  
Juan Chao ◽  
Yu′e Lin ◽  
Qingmin Jin ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Expression Patterns ◽  
Post Inoculation ◽  
Rna Seq ◽  
Disease Resistant ◽  
Meja Treatment ◽  
Gene Transcription Level ◽  
Phytophthora Melonis ◽  
Ja Signaling ◽  
Six Genes

Xu, X., Wang, R., Chao, J., Lin, Y., Jin, Q., He, X., Luo, S. and Wu, T. 2015. The expression patterns of Cucumis sativus WRKY (CsWRKY) family under the condition of inoculation with Phytophthora melonis in disease resistant and susceptible cucumber cultivars. Can. J. Plant Sci. 95: 1121–1131. Based on RNA-seq, we analyzed expression patterns of 55 CsWRKYs in the disease-resistant cultivar (JSH) and the disease-susceptible cucumber cultivar (B80) at 0 and 40 h post-inoculation with Phytophthora melonis. After inoculation with P. melonis, in JSH and B80, the number of up-regulated CsWRKY genes was both 25 with 22 commonly up-regulated genes, and there were 22 CsWRKY genes down-regulated in JSH and 23 in B80 with 18 commonly down-regulated genes, and there were eight CsWRKY genes with no or little change in transcript expression in JSH and seven in B80 with four common genes. Among the 55 CsWRKY genes, 17 genes showed significant differences (differences > twofold) in the degree of change of gene transcription level between JSH and B80 from RNA-seq data analysis, and the results validated using real-time PCR showed that there were only six genes (CsWRKY2, 20, 26, 35, 44, 52) out of the 17 that exhibited significant differences (differences > twofold) with four genes (CsWRKY2, 20, 26, 52) consistent with the data from RNA-seq and two genes (CsWRKY 35, 44) inconsistent with the data from RNA-seq. In addition, the six genes were strongly up-regulated after salicylic acid (SA) treatment with five genes (CsWRKY2, 20, 26, 35, 44) up to peak at 12 h and one gene (CsWRKY52) up to peak at 24 h in expression and that four CsWRKY genes (CsWRKY2, 20, 44, 52) out of six were strongly up-regulated with three genes (CsWRKY2, 20, 44) up to the highest point at 24 h and one gene (CsWRKY52) up after methyl jasmonate (MeJA) treatment. Based on the results above, we predicted CsWRKY2, 20, 26, 35, 44 and 52 may be involved in disease resistance of JSH against Phytophthora melonis by SA and (or) JA signaling pathway(s).

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