Genomic mapping and expression patterns of C380, OK6 and D42 enhancer trap lines in the larval nervous system of Drosophila

2009 ◽  
Vol 9 (5) ◽  
pp. 371-380 ◽  
Author(s):  
Subhabrata Sanyal
Keyword(s):  
Nervous System ◽  
Expression Patterns ◽  
Enhancer Trap ◽  
Genomic Mapping ◽  
Larval Nervous System ◽  
Enhancer Trap Lines

scholarly journals High-resolution analysis of central nervous system expression patterns in zebrafish Gal4 enhancer-trap lines

2015 ◽  
Vol 244 (6) ◽  
pp. 785-796 ◽  
Author(s):  
Hideo Otsuna ◽  
David A. Hutcheson ◽  
Robert N. Duncan ◽  
Adam D. McPherson ◽  
Aaron N. Scoresby ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
High Resolution ◽  
Expression Patterns ◽  
Enhancer Trap ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Enhancer Trap Lines

Functional analysis of GUS expression patterns and T-DNA integration characteristics in rice enhancer trap lines

Plant Science ◽  
2005 ◽  
Vol 168 (6) ◽  
pp. 1571-1579 ◽  
Author(s):  
Hao Peng ◽  
Hongmei Huang ◽  
Yongzhi Yang ◽  
Ying Zhai ◽  
Jinxia Wu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Expression Patterns ◽  
Gus Expression ◽  
Enhancer Trap ◽  
Dna Integration ◽  
Enhancer Trap Lines

scholarly journals Genetic Analysis of Stomatogastric Nervous System Development inDrosophilaUsing Enhancer Trap Lines

1997 ◽  
Vol 186 (2) ◽  
pp. 139-154 ◽  
Author(s):  
Julia Pearl Forjanic ◽  
Chao-Kung Chen ◽  
Herbert Jäckle ◽  
Marcos González Gaitán
Keyword(s):  
Nervous System ◽  
Genetic Analysis ◽  
System Development ◽  
Nervous System Development ◽  
Enhancer Trap ◽  
Stomatogastric Nervous System ◽  
Enhancer Trap Lines

scholarly journals Rimbp, a New Marker for the Nervous System of the Tunicate Ciona robusta

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 1006
Author(s):  
Ugo Coppola ◽  
Paola Olivo ◽  
Enrico D’Aniello ◽  
Christopher J. Johnson ◽  
Alberto Stolfi ◽  
...  
Keyword(s):  
Nervous System ◽  
Active Zone ◽  
Expression Patterns ◽  
Regulatory Elements ◽  
Animal Evolution ◽  
Endogenous Expression ◽  
Presynaptic Mechanisms ◽  
Larval Nervous System ◽  
History Of ◽  
Presynaptic Active Zone

Establishment of presynaptic mechanisms by proteins that regulate neurotransmitter release in the presynaptic active zone is considered a fundamental step in animal evolution. Rab3 interacting molecule-binding proteins (Rimbps) are crucial components of the presynaptic active zone and key players in calcium homeostasis. Although Rimbp involvement in these dynamics has been described in distantly related models such as fly and human, the role of this family in most invertebrates remains obscure. To fill this gap, we defined the evolutionary history of Rimbp family in animals, from sponges to mammals. We report, for the first time, the expression of the two isoforms of the unique Rimbp family member in Ciona robusta in distinct domains of the larval nervous system. We identify intronic enhancers that are able to drive expression in different nervous system territories partially corresponding to Rimbp endogenous expression. The analysis of gene expression patterns and the identification of regulatory elements of Rimbp will positively impact our understanding of this family of genes in the context of Ciona embryogenesis.

scholarly journals Generation of enhancer trap lines inArabidopsisand characterization of expression patterns in the inflorescence

1999 ◽  
Vol 17 (6) ◽  
pp. 699-707 ◽  
Author(s):  
Lauren Campisi ◽  
Yingzhen Yang ◽  
Ying Yi ◽  
Elizabeth Heilig ◽  
Benjamin Herman ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Enhancer Trap ◽  
Enhancer Trap Lines

scholarly journals Partitioning of N-Ethylmaleimide-Sensitive Fusion (NSF) Protein Function in Drosophila melanogaster: dNSF1 Is Required in the Nervous System, and dNSF2 Is Required in Mesoderm

Genetics ◽  
2001 ◽  
Vol 158 (1) ◽  
pp. 265-278
Author(s):  
Jessica A Golby ◽  
Leigh Anna Tolar ◽  
Leo Pallanck
Keyword(s):  
Nervous System ◽  
Protein Function ◽  
Ectopic Expression ◽  
Drosophila Genome ◽  
Loss Of Function ◽  
Stage Of Development ◽  
Expression Studies ◽  
Larval Nervous System ◽  
Constitutive Secretion ◽  
Temporal And Spatial

Abstract The N-ethylmaleimide-sensitive fusion protein (NSF) promotes the fusion of secretory vesicles with target membranes in both regulated and constitutive secretion. While it is thought that a single NSF may perform this function in many eukaryotes, previous work has shown that the Drosophila genome contains two distinct NSF genes, dNSF1 and dNSF2, raising the possibility that each plays a specific secretory role. To explore this possibility, we generated mutations in the dNSF2 gene and used these and novel dNSF1 loss-of-function mutations to analyze the temporal and spatial requirements and the degree of functional redundancy between dNSF1 and dNSF2. Results of this analysis indicate that dNSF1 function is required in the nervous system beginning at the adult stage of development and that dNSF2 function is required in mesoderm beginning at the first instar larval stage of development. Additional evidence suggests that dNSF1 and dNSF2 may play redundant roles during embryonic development and in the larval nervous system. Ectopic expression studies demonstrate that the dNSF1 and dNSF2 gene products can functionally substitute for one another. These results indicate that the Drosophila NSF proteins exhibit similar functional properties, but have evolved distinct tissue-specific roles.

Generation and early differentiation of glial cells in the first optic ganglion of Drosophila melanogaster

Development ◽  
1992 ◽  
Vol 115 (4) ◽  
pp. 903-911 ◽  
Author(s):  
M.L. Winberg ◽  
S.E. Perez ◽  
H. Steller
Keyword(s):  
Drosophila Melanogaster ◽  
Glial Cells ◽  
Enhancer Trap ◽  
P Element ◽  
Cell Divisions ◽  
Genetic Mosaic ◽  
First Optic Ganglion ◽  
Optic Ganglion ◽  
Glial Lineage ◽  
Enhancer Trap Lines

We have examined the generation and development of glial cells in the first optic ganglion, the lamina, of Drosophila melanogaster. Previous work has shown that the growth of retinal axons into the developing optic lobes induces the terminal cell divisions that generate the lamina monopolar neurons. We investigated whether photoreceptor ingrowth also influences the development of lamina glial cells, using P element enhancer trap lines, genetic mosaics and birthdating analysis. Enhancer trap lines that mark the differentiating lamina glial cells were found to require retinal innervation for expression. In mutants with only a few photoreceptors, only the few glial cells near ingrowing axons expressed the marker. Genetic mosaic analysis indicates that the lamina neurons and glial cells are readily separable, suggesting that these are derived from distinct lineages. Additionally, BrdU pulse-chase experiments showed that the cell divisions that produce lamina glia, unlike those producing lamina neurons, are not spatially or temporally correlated with the retinal axon ingrowth. Finally, in mutants lacking photoreceptors, cell divisions in the glial lineage appeared normal. We conclude that the lamina glial cells derive from a lineage that is distinct from that of the L-neurons, that glia are generated independently of photoreceptor input, and that completion of the terminal glial differentiation program depends, directly or indirectly, on an inductive signal from photoreceptor axons.

IDENTIFICATION, SEQUENCE AND EXPRESSION OF A CRUSTACEAN CARDIOACTIVE PEPTIDE (CCAP) GENE IN THE MOTHMANDUCA SEXTA

2001 ◽  
Vol 204 (16) ◽  
pp. 2803-2816 ◽  
Author(s):  
P. K. LOI ◽  
S. A. EMMAL ◽  
Y. PARK ◽  
N. J. TUBLITZ
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Gene Structure ◽  
Expression Patterns ◽  
Amino Acid Residues ◽  
Coding Region ◽  
Genome Database ◽  
Reading Frame ◽  
Crustacean Cardioactive Peptide ◽  
Embryonic Life

SUMMARYThe crustacean cardioactive peptide (CCAP) gene was isolated from the tobacco hawkmoth Manduca sexta. The gene has an open reading frame of 125 amino acid residues containing a single, complete copy of CCAP. Analysis of the gene structure revealed three introns interrupting the coding region. A comparison of the M. sexta CCAP gene with the Drosophila melanogaster genome database reveals significant similarities in sequence and gene structure.The spatial and temporal expression patterns of the CCAP gene in the M. sexta central nervous system were determined in all major post-embryonic stages using in situ hybridization techniques. The CCAP gene is expressed in a total of 116 neurons in the post-embryonic M. sextacentral nervous system. Nine pairs of cells are observed in the brain, 4.5 pairs in the subesophageal ganglion, three pairs in each thoracic ganglion(T1-T3), three pairs in the first abdominal ganglion (A1), five pairs each in the second to sixth abdominal ganglia (A2-A6) and 7.5 pairs in the terminal ganglion. The CCAP gene is expressed in every ganglion in each post-embryonic stage, except in the thoracic ganglia of first- and second-instar larvae. The number of cells expressing the CCAP gene varies during post-embryonic life,starting at 52 cells in the first instar and reaching a maximum of 116 shortly after pupation. One set of thoracic neurons expressing CCAP mRNA shows unusual variability in expression levels immediately prior to larval ecdysis. Using previously published CCAP immunocytochemical data, it was determined that 91 of 95 CCAP-immunopositive neurons in the M. sexta central nervous system also express the M. sexta CCAP gene, indicating that there is likely to be only a single CCAP gene in M. sexta.

Protein expression patterns of identified neurons and of sprouting cells from the leech central nervous system

2000 ◽  
Vol 44 (3) ◽  
pp. 320-332 ◽  
Author(s):  
Xueqing Wu ◽  
Barbara Ritter ◽  
Jan Henrik Schlattjan ◽  
Volkmar Lessmann ◽  
Rolf Heumann ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Protein Expression ◽  
Expression Patterns ◽  
Identified Neurons
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