Novel expression patterns of connexin 30.3 in adult rat cochlea

2010 ◽  
Vol 265 (1-2) ◽  
pp. 77-82 ◽  
Author(s):  
Wen-Hung Wang ◽  
Jiann-Jou Yang ◽  
Yen-Chun Lin ◽  
Jen-Tsung Yang ◽  
Shuan-Yow Li
Keyword(s):  
Expression Patterns ◽  
Adult Rat ◽  
Rat Cochlea

scholarly journals Expression of Gap Junction Protein Connexin43 in the Adult Rat Cochlea: Comparison with Connexin26

2003 ◽  
Vol 51 (7) ◽  
pp. 903-912 ◽  
Author(s):  
Toshihiro Suzuki ◽  
Tetsuro Takamatsu ◽  
Masahito Oyamada
Keyword(s):  
Gap Junction ◽  
Stria Vascularis ◽  
Three Dimensional ◽  
Organ Of Corti ◽  
Structural Difference ◽  
Lateral Wall ◽  
Spiral Ligament ◽  
Adult Rat ◽  
Junction Protein ◽  
Rat Cochlea

To elucidate whether the two different gap junction proteins connexin43 (Cx43) and connexin26 (Cx26) are expressed and localized in a similar manner in the adult rat cochlea, we performed three-dimensional confocal microscopy using cryosections and surface preparations. In the cochlear lateral wall, Cx43-positive spots were localized mainly in the stria vascularis and only a few spots were present in the spiral ligament, whereas Cx26-positive spots were detected in both the stria vascularis and the spiral ligament. In the spiral limbus, Cx43 was widely distributed, whereas Cx26 was more concentrated on the side facing the scala vestibuli and in the basal portion. In the organ of Corti, Cx43-positive spots were present between the supporting cells but they were fewer and much smaller than those of Cx26. These data demonstrated distinct differences between Cx43 and Cx26 in expression and localization in the cochlea. In addition, the area of overlap of zonula occludens-1 (ZO-1) immunolabeling with Cx43-positive spots was small, whereas it was fairly large with Cx26-positive spots in the cochlear lateral wall, suggesting that the differences are not associated with the structural difference between carboxyl terminals, i.e., those of Cx43 possess sequences for binding to ZO-1, whereas those of Cx26 lack these binding sequences.

scholarly journals Immunolocalization of Keratan Sulfate in Rat Spinal Tissues Using the Keratanase Generated BKS-1(+) Neoepitope: Correlation of Expression Patterns with the Class II SLRPs, Lumican and Keratocan

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 826 ◽  
Author(s):  
Anthony J. Hayes ◽  
James Melrose
Keyword(s):  
Spinal Cord ◽  
Vertebral Body ◽  
Core Protein ◽  
Expression Patterns ◽  
Keratan Sulfate ◽  
Protein Species ◽  
Rat Spinal Cord ◽  
Diverse Range ◽  
Adult Rat ◽  
Adult Rat Spinal Cord

This study has identified keratan sulfate in fetal and adult rat spinal cord and vertebral connective tissues using the antibody BKS-1(+) which recognizes a reducing terminal N-acetyl glucosamine-6-sulfate neo-epitope exposed by keratanase-I digestion. Labeling patterns were correlated with those of lumican and keratocan using core protein antibodies to these small leucine rich proteoglycan species. BKS-1(+) was not immunolocalized in fetal spinal cord but was apparent in adult cord and was also prominently immunolocalized to the nucleus pulposus and inner annulus fibrosus of the intervertebral disc. Interestingly, BKS-1(+) was also strongly associated with vertebral body ossification centers of the fetal spine. Immunolocalization of lumican and keratocan was faint within the vertebral body rudiments of the fetus and did not correlate with the BKS-1(+) localization indicating that this reactivity was due to another KS-proteoglycan, possibly osteoadherin (osteomodulin) which has known roles in endochondral ossification. Western blotting of adult rat spinal cord and intervertebral discs to identify proteoglycan core protein species decorated with the BKS-1(+) motif confirmed the identity of 37 and 51 kDa BKS-1(+) positive core protein species. Lumican and keratocan contain low sulfation KS-I glycoforms which have neuroregulatory and matrix organizational properties through their growth factor and morphogen interactive profiles and ability to influence neural cell migration. Furthermore, KS has interactive capability with a diverse range of neuroregulatory proteins that promote neural proliferation and direct neural pathway development, illustrating key roles for keratocan and lumican in spinal cord development.

Expression patterns of Nedd4 isoforms and SGK1 in the rat cochlea

2009 ◽  
Vol 129 (9) ◽  
pp. 935-939 ◽  
Author(s):  
Shi-Xun Zhong ◽  
Zhao-Hua Liu
Keyword(s):  
Expression Patterns ◽  
Rat Cochlea

Developmental expression patterns of connexin26 and -30 in the rat cochlea

1999 ◽  
Vol 25 (4) ◽  
pp. 306-311 ◽  
Author(s):  
J�rgen Lautermann ◽  
H.-G. Frank ◽  
Klaus Jahnke ◽  
Otto Traub ◽  
Elke Winterhager
Keyword(s):  
Expression Patterns ◽  
Developmental Expression ◽  
Rat Cochlea

Identification of a novel alternative splicing form of human netrin-4 and analyzing the expression patterns in adult rat brain

2004 ◽  
Vol 130 (1-2) ◽  
pp. 68-80 ◽  
Author(s):  
Chenggang Zhang ◽  
Fanwei Meng ◽  
Chunli Wang ◽  
Huiyun Guo ◽  
Ming Fan ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Rat Brain ◽  
Expression Patterns ◽  
Adult Rat ◽  
Adult Rat Brain

Notch2: a second mammalian Notch gene

Development ◽  
1992 ◽  
Vol 116 (4) ◽  
pp. 931-941 ◽  
Author(s):  
G. Weinmaster ◽  
V.J. Roberts ◽  
G. Lemke
Keyword(s):  
Cell Fate ◽  
Expression Patterns ◽  
In Situ Hybridisation ◽  
Cell Surface Receptor ◽  
Cellular Interactions ◽  
Spatial And Temporal Patterns ◽  
Cell Fate Decisions ◽  
Adult Rat ◽  
Surface Receptor ◽  
Notch Gene

Notch is a cell surface receptor that mediates a wide variety of cellular interactions that specify cell fate during Drosophila development. Recently, homologs of Drosophila Notch have been isolated from Xenopus, human and rat, and the expression patterns of these vertebrate proteins suggest that they may be functionally analogous to their Drosophila counterpart. We have now identified a second rat gene that exhibits substantial nucleic and amino acid sequence identity to Drosophila Notch. This gene, designated Notch2, encodes a protein that contains all the structural motifs characteristic of a Notch protein. Thus, mammals differ from Drosophila in having more than one Notch gene. Northern and in situ hybridisation analyses in the developing and adult rat identify distinct spatial and temporal patterns of expression for Notch1 and Notch2, indicating that these genes are not redundant. These results suggest that the great diversity of cell-fate decisions regulated by Notch in Drosophila may be further expanded in vertebrates by the activation of distinct Notch proteins.

Constitutive expression of cyclooxygenase-2 in rat vas deferens

1998 ◽  
Vol 275 (1) ◽  
pp. R227-R233 ◽  
Author(s):  
James A. McKanna ◽  
Ming-Zhi Zhang ◽  
Jun-Ling Wang ◽  
H.-F. Cheng ◽  
Raymond C. Harris
Keyword(s):  
Vas Deferens ◽  
Expression Patterns ◽  
Constitutive Expression ◽  
The Body ◽  
Northern Analysis ◽  
Adult Rat ◽  
Uterine Muscle ◽  
Organ Systems ◽  
Mammalian Tissues ◽  
Cox 2

Prostaglandins, lipoid substances discovered in human semen as modulators of uterine muscle contractility, are known to play significant roles in virtually all mammalian organ systems, but their male reproductive functions are unclear. Cyclooxygenase, the rate-limiting enzyme in prostaglandin synthesis, occurs in two isoforms distinguished on the basis of constitutive (COX-1) or inducible (COX-2) expression patterns in mammalian tissues. However, in the adult rat male reproductive system, immunohistochemistry and Western and Northern analysis showed that COX-2 is the predominant isoform and is heavily localized to the epithelium of the distal vas deferens, where constitutive expression is manyfold greater than in any other organs of the body. COX-2 is not detected in the proximal one-half of the vas nor in the testis, epididymis, seminal vesicles, or prostate. Elimination of luminal sperm by vasectomy does not affect COX-2 levels, whereas castration severely depletes COX-2 and androgen replacement after castration restores COX-2, indicating that COX-2 expression in the vas is androgen dependent. Because the distal vas also comprises an extensive submucosal venous plexus connected to the penile corpora cavernosa, prostaglandins from the vas may play a role in erection.

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