Advances in pullulan production from agro-based wastes by Aureobasidium pullulans and its applications

AbstractUtilization of biocontrol agents is a sustainable approach to reduce plant diseases caused by fungal pathogens. In the present study, we tested the effect of the candidate biocontrol fungus Aureobasidium pullulans (De Bary) G. Armaud on strawberry under in vitro and in vivo conditions to control crown rot, root rot and grey mould caused by Phytophthora cactorum (Lebert and Cohn) and Botrytis cinerea Pers, respectively. A dual plate confrontation assay showed that mycelial growth of P. cactorum and B. cinerea was reduced by 33–48% when challenged by A. pullulans as compared with control treatments. Likewise, detached leaf and fruit assays showed that A. pullulans significantly reduced necrotic lesion size on leaves and disease severity on fruits caused by P. cactorum and B. cinerea. In addition, greenhouse experiments with whole plants revealed enhanced biocontrol efficacy against root rot and grey mould when treated with A. pullulans either in combination with the pathogen or pre-treated with A. pullulans followed by inoculation of the pathogens. Our results demonstrate that A. pullulans is an effective biocontrol agent to control strawberry diseases caused by fungal pathogens and can be an effective alternative to chemical-based fungicides.

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Investigating the effects of Aureobasidium pullulans on grape juice composition and fermentation

Food Microbiology ◽

10.1016/j.fm.2020.103451 ◽

2020 ◽

Vol 90 ◽

pp. 103451 ◽

Cited By ~ 5

Author(s):

Cristobal A. Onetto ◽

Anthony R. Borneman ◽

Simon A. Schmidt

Keyword(s):

Aureobasidium Pullulans ◽

Grape Juice

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Chemical improvement of surfaces. Part 3: Covalent modification of Scots pine sapwood with substituted benzoates providing resistance to Aureobasidium pullulans staining fungi

Holzforschung ◽

10.1515/hf-2014-0086 ◽

2015 ◽

Vol 69 (5) ◽

pp. 595-601 ◽

Cited By ~ 4

Author(s):

Jan C. Namyslo ◽

Dieter E. Kaufmann ◽

Carsten Mai ◽

Holger Militz

Keyword(s):

Scots Pine ◽

Aureobasidium Pullulans ◽

Covalent Modification ◽

Attenuated Total Reflection ◽

Chemically Modified ◽

Covalently Bonded ◽

Blue Stain ◽

Natural Wood ◽

The Individual ◽

Modified Wood

Abstract The development of appropriate chemical precursors that can covalently functionalize natural wood aims at efficient restriction of deterioration. Biological staining experiments were performed with veneer pieces made of sapwood of Scots pine (Pinus sylvestris L.) that had previously been chemically modified with substituted benzoates. Based on the recently published protocol on esterification of wood by means of 1H-benzotriazole activation, the quantity of covalently bonded organomaterials (QCOs), a recently defined advantageous value considering the individual molecular weight of the functionalizing organochemical groups, was obtained in the range of 0.9–1.5 mmol g-1. The modified wood was analyzed by attenuated total reflection IR spectroscopy. Modification with three electronically different benzoates clearly reduced the colonization of the specimen’s surfaces by the blue stain fungus Aureobasidium pullulans but did not fully prevent it. The degree of colonization appeared to decrease with increasing QCO values of the modification agents but apparently did not strongly depend on the additional functionality of the benzoate.

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