Analysis of the water-soluble polysaccharides from Camellia japonica pollen and their inhibitory effects on galectin-3 function

2020 ◽  
Vol 159 ◽  
pp. 455-460 ◽  
Author(s):  
Limei Zhou ◽  
Pengcheng Ma ◽  
Ming Shuai ◽  
Jian Huang ◽  
Chengxin Sun ◽  
...  
Keyword(s):  
Water Soluble ◽  
Inhibitory Effects ◽  
Camellia Japonica ◽  
Galectin 3

Disparate effects of two phosphatidylcholine binding proteins, C-reactive protein and surfactant protein A, on pulmonary surfactant structure and function

2004 ◽  
Vol 287 (6) ◽  
pp. L1145-L1153 ◽  
Author(s):  
Kaushik Nag ◽  
Karina Rodriguez-Capote ◽  
Amiya Kumar Panda ◽  
Laura Frederick ◽  
Stephen A. Hearn ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Amorphous Material ◽  
Protein A ◽  
Surfactant Protein ◽  
Water Soluble ◽  
Surfactant Protein A ◽  
Surface Films ◽  
Inhibitory Effects ◽  
C Reactive Protein ◽  
Reactive Protein

C-reactive protein (CRP) and surfactant protein A (SP-A) are phosphatidylcholine (PC) binding proteins that function in the innate host defense system. We examined the effects of CRP and SP-A on the surface activity of bovine lipid extract surfactant (BLES), a clinically applied modified natural surfactant. CRP inhibited BLES adsorption to form a surface-active film and the film's ability to lower surface tension (γ) to low values near 0 mN/m during surface area reduction. The inhibitory effects of CRP were reversed by phosphorylcholine, a water-soluble CRP ligand. SP-A enhanced BLES adsorption and its ability to lower γ to low values. Small amounts of SP-A blocked the inhibitory effects of CRP. Electron microscopy showed CRP has little effect on the lipid structure of BLES. SP-A altered BLES multilamellar vesicular structure by generating large, loose bilayer structures that were separated by a fuzzy amorphous material, likely SP-A. These studies indicate that although SP-A and CRP both bind PC, there is a difference in the manner in which they interact with surface films.

scholarly journals Water soluble polysaccharides from Spirulina platensis exhibited cyto/DNA protection and suppress the growth of gastric cancer cells via modulation of galectin-3

2021 ◽  
Author(s):  
Vinayak Uppin ◽  
Shylaja M Dharmesh ◽  
Sarada R
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
In Silico ◽  
Average Molecular Weight ◽  
Haemagglutination Inhibition ◽  
Water Soluble ◽  
Gastric Cancer Cells ◽  
Galectin 3 ◽  
Nih 3T3

Polysaccharides from natural sources play a significant role in the management of different cancer types including gastric cancer. In this study, we reported the effect of spirulina polysaccharide (Sp) on galectin-3 modulatory activity on gastric cancer cells. The polysaccharide was isolated from the spirulina biomass, characterized, and the in silico, in vitro studies are carried out to assess the bioactivities. The isolated Sp possessed average molecular weight of 1457 kDa, and galactose (42%) as major sugar along with Rhamnose, Arabinose, Xylose, and Mannose. Further, characterization of Sp by FT-IR and NMR spectrum indicated the presence of (β1-4D) galactose sugar with galactoarabinorhamnoglycan backbone. Among the monosaccharides, galactose showed highest binding affinity with galectin-3 protein as evidenced by the in silico interaction study. The obtained Sp, inhibited the proliferation of AGS gastric cancer cells by 48 % without affecting normal NIH/3T3 cells as opposed to doxorubicin, a known anticancer drug. Also, Sp exhibited galectin-3 mediated haemagglutination inhibition with MIC of 9.37 μg/mL compared to galactose 6.25 μg/mL, sugar specific to galectin-3. The Sp treatment significantly (p<0.05) lowered the expression of galectin-3 by 32 % compared to untreated control cells. In addition, Sp exhibited the potent cytoprotection in RBCs, Buccal cells, and DNA exposed to oxidants. Thus, the findings suggest that the polysaccharide from spirulina offer a promising therapeutic strategy in the management of gastric cancer in addition to its currently known nutritional and pharmaceutical applications.

scholarly journals Quaternary Salts of Chitosan: History, Antimicrobial Features, and Prospects

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Douglas de Britto ◽  
Rejane Celi Goy ◽  
Sergio Paulo Campana Filho ◽  
Odilio B. G. Assis
Keyword(s):  
Physical Chemistry ◽  
Antimicrobial Activity ◽  
Drug Delivery Systems ◽  
Antimicrobial Properties ◽  
Water Soluble ◽  
Chemical Characteristics ◽  
Packaging Materials ◽  
Inhibitory Effects ◽  
Quaternary Salts ◽  
Derivatives Of

Recently, increasing attention has been paid to water-soluble derivatives of chitosan at its applications. The chemical characteristics and the antimicrobial properties of these salts can play significant role in pharmacological and food areas mainly as carriers for drug delivery systems and as antimicrobial packaging materials. In the current paper, a historical sequence of the main preparative methods, physical chemistry aspects, and antimicrobial activity of chitosan quaternized derivatives are presented and briefly discussed. In general, the results indicated that the quaternary derivatives had better inhibitory effects than the unmodified chitosan.

scholarly journals Phytotoxic Effect of Tithonia rotundifolia (Miller) S.F.Blake on Chlorophyll and Protein Contents of Vigna unguiculata L. and Zea mays L.

2019 ◽  
Vol 10 (2) ◽  
pp. 79
Author(s):  
Ilori Olasupo John ◽  
Olutobi Oluwafunmilayo Otusanya
Keyword(s):  
Zea Mays ◽  
Vigna Unguiculata ◽  
Zea Mays L ◽  
Water Soluble ◽  
Inhibitory Effects ◽  
Potted Plants ◽  
Phytotoxic Effect ◽  
Biochemical Analyses ◽  
Germination And Growth ◽  
Range Test

A large number of plants impose inhibitory effects on the germination and growth of neighbouring or successional plants by releasing allelochemicals into the soil. This study investigated the phytotoxic effects of Tithonia rotundifolia (Miller) S.F.Blake on the chlorophyll and protein contents of Vigna unguiculata (L.) Walpers and Zea mays L.. This was with a view to determining the susceptibility of these crops to allelochemicals in the extracts prepared from T. rotundifolia. Seeds of the test plants were sown in pots filled with top humus soil. At two weeks, seedlings in each pot were thinned down to 10 seedlings per pot. Potted plants of the test crops were supplied with 400 ml of the appropriate water extracts while the control potted plants were supplied with 400 ml of water. Biochemical analyses were carried out according to standard methods. The data obtained were analysed by (ANOVA) to determine significant (P< 0.05) effects. The means were compared using Duncan Multiple Range Test. The chlorophyll a, chlorophyll b, total chlorophyll and protein contents in V. unguiculata and Z. mays and were significantly inhibited by the extract from T. rotundifolia. The inhibitory effects of these allelochemicals increased with concentration. The phytotoxic effect of T. rotundifolia was species dependent. It was concluded that the extract contains water-soluble allelochemicals which inhibited the biochemical parameters of the test crops. T. rotundifolia should be controlled where it grows in association with cultivated crops.

scholarly journals Medicinal Flowers. XXXVI.1) Acylated Oleanane-Type Triterpene Saponins with Inhibitory Effects on Melanogenesis from the Flower Buds of Chinese Camellia japonica

2012 ◽  
Vol 60 (6) ◽  
pp. 752-758 ◽  
Author(s):  
Seikou Nakamura ◽  
Katsuyoshi Fujimoto ◽  
Souichi Nakashima ◽  
Takahiro Matsumoto ◽  
Tomoko Miura ◽  
...  
Keyword(s):  
Triterpene Saponins ◽  
Inhibitory Effects ◽  
Flower Buds ◽  
Camellia Japonica

Inhibition of Platelet Adhesiveness and Aggregation by Benzyl Alcohol and Phenol

1969 ◽  
Vol 21 (02) ◽  
pp. 362-366 ◽  
Author(s):  
A.J Zweifler ◽  
S.S Sanbar
Keyword(s):  
Platelet Aggregation ◽  
Benzyl Alcohol ◽  
Local Anesthetic ◽  
Potent Inhibitor ◽  
Adenosine Diphosphate ◽  
Glass Beads ◽  
Water Soluble ◽  
Platelet Adhesiveness ◽  
Inhibitory Effects

SummaryTwo amphipathic alcohols, benzyl alcohol and phenol, have been found to diminish adhesiveness of platelets to glass beads and to impair adenosine diphosphate (ADP)-induced platelet aggregation, while ethanol and isopropanol, both completely water soluble, had no inhibitory effects. Comparatively, phenol is a more potent inhibitor than benzyl alcohol. It is suggested that the basis for their antiadhesive effect may be similar to that which underlies their local anesthetic properties.

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