Nostoc flagelliforme capsular polysaccharides from different culture conditions improve hyperlipidemia and regulate intestinal flora in C57BL/6J mice to varying degrees

2022 ◽  
Author(s):  
Jian Li ◽  
Shi-gang Shen ◽  
Cheng-feng Han ◽  
Shu-ting Liu ◽  
Le-le Zhang ◽  
...  
Keyword(s):  
Intestinal Flora ◽  
Culture Conditions ◽  
Capsular Polysaccharides ◽  
Nostoc Flagelliforme

Heterotrophic Fed-Batch Cultures of Nostoc flagelliforme and Production of Extracellular Polysaccharides

2012 ◽  
Vol 518-523 ◽  
pp. 5468-5471
Author(s):  
Shi Ru Jia ◽  
Zhen Ding ◽  
Ning Tan ◽  
Nan Wang ◽  
Pei Pei Han ◽  
...  
Keyword(s):  
Batch Culture ◽  
Economic Value ◽  
Culture Conditions ◽  
Extracellular Polysaccharides ◽  
Nostoc Flagelliforme ◽  
Fed Batch ◽  
Batch Cultures ◽  
Dissociated Cells ◽  
Fed Batch Culture ◽  
Different Cultures

Nostoc flagelliforme is a kind of terrestrial cyanobacterium with high economic value. Dissociated cells, which separated from a natural colony of N. flagelliforme, could be cultivated heterotrophically in the darkness on xylose and glucose under fed-batch culture conditions. Growth and extracellular polysaccharides (EPS) production in different cultures are investigated. At harvest time, the cultures contain 1.215 g•L-1 of biomass and 122.5 mg•L-1 of EPS respectively. The gravimetric EPS production rate is 17.5 mg•g-1•day-1, which is 1.65 times higher than previously reported results for heterotrophic Nostoc flagelliforme grown on xylose batch culture.

THE MORPHOGENESIS OF NOSTOC FLAGELLIFORME UNDER CULTURE CONDITIONS

2010 ◽  
Vol 36 (2) ◽  
pp. 323-329 ◽  
Author(s):  
Xue-Min ZHAO ◽  
Yong-Hong BI ◽  
Guang-Jie ZHOU ◽  
Zheng-Yu HU
Keyword(s):  
Culture Conditions ◽  
Nostoc Flagelliforme

scholarly journals Influence of culture conditions on extracellular polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Nostoc flagelliforme

RSC Advances ◽  
2017 ◽  
Vol 7 (71) ◽  
pp. 45075-45084 ◽  
Author(s):  
Pei-pei Han ◽  
Shun-yu Yao ◽  
Rong-jun Guo ◽  
Rong-rong Yan ◽  
Yi-kai Wu ◽  
...  
Keyword(s):  
Extracellular Polysaccharide ◽  
Culture Conditions ◽  
Nostoc Flagelliforme ◽  
Polysaccharide Production ◽  
Polysaccharide Synthesis

Important enzymes influencing the production ofNostoc flagelliformeEPS were investigated under different culture conditions.

scholarly journals TLR2-stimulating contaminants in glycoconjugate fractions prepared from Bacteroides fragilis

2017 ◽  
Vol 23 (5) ◽  
pp. 449-458 ◽  
Author(s):  
Masahito Hashimoto ◽  
Junpei Waki ◽  
Haruyuki Nakayama-Imaohji ◽  
Mami Ozono ◽  
Shuhei Hashiguchi ◽  
...  
Keyword(s):  
Capsular Polysaccharide ◽  
Intestinal Flora ◽  
Bacteroides Fragilis ◽  
Hydrophobic Interaction Chromatography ◽  
Hot Water ◽  
Proteinase K ◽  
Capsular Polysaccharides ◽  
Wild Type ◽  
Almost All ◽  
Cell Surface Glycoconjugates

Bacteroides fragilis is a member of the normal intestinal flora and is involved in host immunostimulation via TLR2. On the bacterial cell surface, glycoconjugates, such as LPS and capsular polysaccharide A (PSA), have been reported to participate in host immunostimulation via TLR2. Previously, we identified a TLR2-stimulating lipoprotein in B. fragilis cells. In this study, we demonstrated that TLR2-stimulating principal molecules in glycoconjugate fractions prepared from B. fragilis are contaminating proteinous molecules, which may also be lipoproteins. The glycoconjugate fractions were prepared by phenol–hot water extraction of B. fragilis wild type and PSA-deficient strains, followed by hydrophobic interaction chromatography. TLR2-stimilating activities of the fractions were not affected by PSA deficiency. By in-gel TLR2-stimulation assay, molecules in high-molecular-mass area, where capsular polysaccharides were migrated, were found not to stimulate TLR2, but those in the range of 15–40 kDa were active. Further, proteinase K could digest the latter molecules and the TLR2-stimulating activities were migrated to the area of below 15 kDa. These results support that proteinous molecules, which are estimated to be lipoproteins, are responsible for almost all TLR2-stimulating activity in the glycoconjugate fractions prepared from B. fragilis.

scholarly journals Comparisons of Functional Properties of Polysaccharides from Nostoc flagelliforme under Three Culture Conditions

Polymers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 263 ◽  
Author(s):  
Shi-gang Shen ◽  
Ya-hui Lin ◽  
Dong-xue Zhao ◽  
Yi-kai Wu ◽  
Rong-rong Yan ◽  
...  
Keyword(s):  
Salt Stress ◽  
Functional Properties ◽  
Binding Capacity ◽  
Culture Conditions ◽  
Specific Yield ◽  
Nostoc Flagelliforme ◽  
Mixotrophic Culture ◽  
Emulsification Activity ◽  
Foaming Property ◽  
Mineral Industries

Nostoc flagelliforme is an edible cyanobacterium with excellent food and herbal values. It has been used as food in China for more than 2000 years. Many studies have been focused on improving the yield and bioactivity of Nostoc flagelliforme polysaccharides although these have ignored the functional properties. In this study, we extracted and purified three polysaccharides (WL-CPS, NaCl-CPS and Glu-CPS) from Nostoc flagelliforme under normal, salt stress and mixotrophic culture conditions, respectively, in order to change the physicochemical properties of polysaccharides with the aim of obtaining better functional properties. Both salt stress and mixotrophic culture conditions increased the specific yield of polysaccharides. Their functional properties were comparatively investigated and the results showed that NaCl-CPS exhibited the highest emulsification activity and flocculation capability, which was also higher than that of some commercial products. In contrast, Glu-CPS exhibited the highest water and oil holding capacities, foaming property, intrinsic viscosity and bile acids binding capacity. Our results indicated that both NaCl-CPS and Glu-CPS could be considered to be functional polysaccharides according to their respective characteristics, which have great potential in numerous applications, such as food, pharmaceutical, cosmetic, chemical and mineral industries. These findings also demonstrated the potential application of the proper regulation of culture conditions in the development of polysaccharides with desired functional properties.

Ultrastructure of the effect of T-514 obtained from Karwinskia humboldtiana on assembly and integrity of yeast's peroxisomes

1991 ◽  
Vol 49 ◽  
pp. 136-137
Author(s):  
J. Sepulveda-Saavedra ◽  
I. Vander-Klei ◽  
M. Venhuis ◽  
Y. Piñeyro-Lopez
Keyword(s):  
Culture Conditions ◽  
Toxic Effects ◽  
Candida Boidinii ◽  
Central Mexico ◽  
Poisonous Plant ◽  
Biological Behaviour ◽  
Enzyme Content ◽  
Fat Deposits ◽  
Yeast Model

Karwinskia humboldtiana is a poisonous plant that grows in semi desertic areas in north and central México. It produces several substances with different toxic effects. One of them designated T-514 damages severely the lung, kidney and liver, producing in the hepatoeyte large intracellular fat deposits and necrosis. Preliminary observations demonstrated that three is a decrease in the amount of peroxisomes in the hepatocytes of experimentally intoxicated rats and monkeys. To study the effect exerted by the T-514 on peroxisomes, a yeast model was selected, thus, three species: Saccha romices cerevisiae, Ilansenula polymorpha and Candida boidinii were used, because there is information concerning their peroxisome's morphology, enzyme content, biological behaviour under different culture conditions and biogenesis.

Scanning Microscopy of Human Intestinal Explants in Organ Culture

1972 ◽  
Vol 30 ◽  
pp. 396-397
Author(s):  
Bruce Wetzel ◽  
Robert Buscho ◽  
Raphael Dolin
Keyword(s):  
Electron Microscopy ◽  
Room Temperature ◽  
Culture Conditions ◽  
Human Fetuses ◽  
Enteric Disease ◽  
Organ Cultures ◽  
Growth Of A Variety ◽  
Normal Human ◽  
Fetal Intestine ◽  
Scanning Electron

It has been reported that explants of human fetal intestine can be maintained in culture for up to 21 days in a viable condition and that these organ cultures support the growth of a variety of known viral agents responsible for enteric disease. Scanning electron microscopy (SEM) has been undertaken on several series of these explants to determine their appearance under routine culture conditions.Fresh specimens of jejunum obtained from normal human fetuses were washed, dissected into l-4mm pieces, and cultured in modified Leibowitz L-15 medium at 34° C as previously described. Serial specimens were fixed each day in 3% glutaraldehyde for 90 minutes at room temperature, rinsed, dehydrated, and dried by the CO2 critical point method in a Denton DCP-1 device. Specimens were attached to aluminum stubs with 3M transfer tape No. 465, and one sample on each stub was carefully rolled along the adhesive such that villi were broken off to expose their interiors.

Lectin Binding Studies on RNA Tumor Virus-Infected Cells

1975 ◽  
Vol 33 ◽  
pp. 326-327
Author(s):  
D. C. Hixson
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Culture Conditions ◽  
3T3 Cells ◽  
Binding Studies ◽  
Con A ◽  
Microscopic Studies ◽  
Tumor Virus ◽  
Infected Cells ◽  
Cell Culture Conditions

The abilities of plant lectins to preferentially agglutinate malignant cells and to bind to specific monosaccharide or oligosaccharide sequences of glycoproteins and glycolipids make them a new and important biochemical probe for investigating alterations in plasma membrane structure which may result from malignant transformation. Electron and light microscopic studies have demonstrated clustered binding sites on surfaces of SV40-infected or tryp- sinized 3T3 cells when labeled with concanavalin A (con A). No clustering of con A binding sites was observed in normal 3T3 cells. It has been proposed that topological rearrangement of lectin binding sites into clusters enables con A to agglutinate SV40-infected or trypsinized 3T3 cells (1). However, observations by other investigators have not been consistent with this proposal (2) perhaps due to differences in reagents used, cell culture conditions, or labeling techniques. The present work was undertaken to study the lectin binding properties of normal and RNA tumor virus-infected cells and their associated viruses using lectins and ferritin-conjugated lectins of five different specificities.

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