Prevalence of HPV infection by cytologic diagnosis and HPV DNA extraction and prevalence of the HPV genotypes detected in urban Turkish women

Worldwide, 96,000 cases of oropharyngeal cancer (OPC) occurred in 2012. Human papillomavirus (HPV) is a risk factor for OPC. Data on oropharyngeal HPV infection are limited. There is no consensus on the best sampling method for detecting the infection. We describe the prevalence of oropharyngeal HPV infection among HIV-infected men and compare the performance of oral rinses and swabs in detecting oropharyngeal HPV infection. Paired oral rinses and swabs for 181 men were tested for HPV DNA using the Roche Linear Array. Performance was determined by the number of infections detected and the percentage of samples with adequate DNA extraction. Agreement between sampling methods was assessed by the kappa statistic. Prevalence of oropharyngeal HPV infection with rinse samples was 1.8% (three infections) and 0.6% (one infection) with swabs (p = 0.06). Adequate cellular DNA extraction was more likely with rinse (93.4%) than swab samples (89.0%, p = 0.05). There was moderate agreement between the methods (kappa = 0.49). The prevalence of oropharyngeal HPV DNA infection among this predominantly heterosexual sample of men living with HIV was low and consistent with the infrequent oral sex practices. Oral rinse performed better than oral swab in detecting oropharyngeal HPV DNA infection and might contribute to screening for OPCs.

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Human papilloma virus (HPV) genotypes concordance between Iranian couples referrals

Infectious Agents and Cancer ◽

10.1186/s13027-019-0241-x ◽

2019 ◽

Vol 14 (1) ◽

Author(s):

Mehrdad Davarmanesh ◽

Mehrouz Dezfulian ◽

Mohammad Javad Gharavi ◽

Sarang Younesi ◽

Pourandokht Saadati ◽

...

Keyword(s):

Human Papilloma Virus ◽

Hpv Vaccination ◽

Hpv Infection ◽

Sexual Partners ◽

Papilloma Virus ◽

Hpv Dna ◽

Hpv Genotypes ◽

Sexual Couples ◽

Males And Females ◽

The Mean

Abstract Background Human Papilloma Virus (HPV) genotypes concordance among sexual couples has been evaluated in many investigations with considerable variations in the concordance. However, no such study has carried out between Iranian couples yet. Methods Urogenital specimen from both males and females of couples were taken and transferred to Nilou laboratory for molecular analysis. HPV DNA extraction and typing were carried out using cobas 4800 platform. Demographic and virological data were analyzed afterwards. Results One hundred fourteen couples were enrolled in the study. The mean age of participants were 36 ± 8 and 32 ± 7 for males and females, respectively. 64 (28%) of specimens were positive for at least one HPV genotype. The positive rates within genders were 30.7 and 25.4% for females and males, respectively with a considerable association (P value 0.021). Within the positive samples, 13(5.7%), 8 (7%) and 31(13.5%) were belonged to 16, 18 and other HR genotypes. 59 (51.8%) couples who were negative for HPV showed negative concordance. Of the total positive HPV patients (55 couples, 48.2%), 9 (16.3%) couples had positive concordance and the rest of 46 (83.7%) couples (either of spouse being negative and the other being positive for HPV) showed neither kinds of concordance. Conclusion Recognition of the dynamics of HPV infection not only in women, but in their sexual partners could impact the implementation of preventive measures like HPV vaccination for cervical cancer and other HPV-related diseases for both sexual partners.

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Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin Women

Acta Medica (Hradec Kralove Czech Republic) ◽

10.14712/18059694.2015.50 ◽

2012 ◽

Vol 55 (3) ◽

pp. 130-132 ◽

Cited By ~ 1

Author(s):

Danijela Vujošević ◽

Vineta Vuksanović ◽

Mario Poljak ◽

Nebojša Jokmanović

Keyword(s):

Human Papillomavirus ◽

Hpv Infection ◽

High Grade ◽

Specific Primers ◽

Hpv Dna ◽

Younger Women ◽

Hpv Genotypes ◽

Pcr Products ◽

Hpv Types ◽

High Risk Hpv

The risk of high-grade lesions and carcinoma is known to correlate with specific high-risk HPV genotypes. The distribution of HPV types varies between countries and little is known about HPV distribution in our country. Therefore, the purpose of this study was to determine the range and frequency of HPV genotypes in studied group of women in Montenegro. HPV genotypes were determined using the method of enzyme restriction of PCR products amplified with group-specific primers MY09/MY11 and restricted with seven different restriction endonucleases. Out of the total number of women HPV infection was found in 1/5 of participants (20%). Genotyping performed in HPV DNA positive women shows that the HPV genotype 16 is dominant and present in more than 1/3 of the participants (36.8%). The second most frequent HPV infection is with HPV genotype 58 and it is found in 10.5% of participants. HPV 31 and HPV 6 infections are present in 7.9% of women, while infections with other genotypes were demonstrated individually by 2.6%. Multiple HPV infection was demonstrated in 18.4% and they are dominant in younger women (aged 25 to 30 years).

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HPV infection in semen: results from a new molecular approach

Epidemiology and Infection ◽

10.1017/s0950268819000621 ◽

2019 ◽

Vol 147 ◽

Cited By ~ 5

Author(s):

G. Capra ◽

R. Schillaci ◽

L. Bosco ◽

M. C. Roccheri ◽

A. Perino ◽

...

Keyword(s):

Clinical Manifestations ◽

Hpv Infection ◽

Viral Dna ◽

New Approach ◽

Hpv Dna ◽

Sexually Transmitted ◽

Viral Genomes ◽

Hpv Genotypes ◽

Oncogenic Hpv

AbstractHuman papillomavirus (HPV) is the agent of the most common sexually transmitted diseases causing a variety of clinical manifestations ranging from warts to cancer. Oncogenic HPV infection is the major cause of cervical cancer and less frequently of penile cancers. Its presence in semen is widely known, but the effects on fertility are still controversial. We developed a new approach to evaluate virus localisation in the different semen components. We analysed also the specific genotype localisation and viral DNA quantity by qPCR. Results show that HPV DNA can be identified in every fraction of semen: spermatozoa, somatic cells and seminal plasma. Different samples can contain the HPV DNA in different fractions and several HPV genotypes can be found in the same fraction. Additionally, different fractions may contain multiple HPV genotypes in different relative quantity. We analysed the wholeness of HPV DNA in sperm cells by qPCR. In one sample more than half of viral genomes were defective, suggesting a possible recombination event. The new method allows to easily distinguish different sperm infections and to observe the possible effects on semen. The data support the proposed role of HPV in decreased fertility and prompt new possible consequences of the infection in semen.

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Retrospective Analysis of Prevalence of High-risk and Low-risk Human Papillomavirus (HPV) Genotypes in Iranian Women During 2013-2016

Asian Pacific Journal of Cancer Biology ◽

10.31557/apjcb.2017.2.4.85-90 ◽

2017 ◽

Vol 2 (4) ◽

pp. 85-90 ◽

Cited By ~ 1

Author(s):

Siavash Chalabiani ◽

Mina Khodadad Nazari ◽

Mahdi Shabani ◽

Neda Razavi Davoodi ◽

Abdolfattah Sarafnejad ◽

...

Keyword(s):

High Risk ◽

Age Groups ◽

Hpv Infection ◽

Low Risk ◽

Age Group ◽

Cancer Etiology ◽

Cross Sectional ◽

Hpv Dna ◽

Hpv Prevalence ◽

Hpv Genotypes

Background and objective: Considering the importance of HPV in cancer etiology, awareness of HPV prevalence and frequency of high-risk genotypes could help improve health care system management. We analyzed HPV prevalence in women forms different provinces of Iran that is the largest sample till now. Material and Methods: In this retrospective cross-sectional study, 2969 outpatient and suspicious women aged 17- 78 referred to Noor pathobiology laboratory from 24 provinces of Iran were studies. DNA extraction and PCR were performed on samples and then HPV genotypes were also determined using hybridization kit. Results: HPV DNA was detected in 29.3% of valid liquid-based samples. Among HPV positive cases, 67.2% and 52.0% accounted for high-risk and low-risk HPV subtypes, respectively. In patients with high-risk HPV types, HPV16 was confirmed as predominate type (30.5%) followed by HPV53 (17.3%) and HPV39 (13.3 %). HPV6 was found as the most common low-risk HPV type with 60.6% frequency rate followed by HPV11 (17.9%) and HPV81 (8.6%) in this age group. It was apparent that age group >25 years accounted the highest frequency of HPV positivity. The prevalence of HPV was significantly different among different age groups (p<0.0001). Conclusion: It can be concluded that HPV infection is currently at a considerable high level in Iran. Looking at the high risk and oncogenic HPV subtypes frequency especially in younger age groups, a concern could be noticed about HPV relevant cancers which can be prevented by commercial and approved HPV vaccines.

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Epidemiological Study of Various HPV Strains in Cervical Fluid Samples in South-Eastern Iran, 2018–2020

Archives of Iranian Medicine ◽

10.34172/aim.2021.97 ◽

2021 ◽

Vol 24 (9) ◽

pp. 678-683

Author(s):

Reza Bahramabadi ◽

Zahra Honarvar ◽

Maryam Iranpour ◽

Mohammad Kazemi Arababadi ◽

Tania Dehesh ◽

...

Keyword(s):

Hpv Infection ◽

Cross Sectional Study ◽

Cervical Cancer Prevention ◽

Cross Sectional ◽

Hpv Dna ◽

Service Programs ◽

Eastern Iran ◽

Hpv Genotypes ◽

East Of Iran ◽

Multiple Strains

Background: Uterine cervical malignancy is one of the commonly detected malignancies related to the human papillomavirus (HPV) and is increasing incidentally in developing countries. Therefore, the use of an efficient diagnostic method is required as an effectual step for cervical cancer prevention and treatment. The purpose of the study was to diagnose various types of HPV in the cervical cytology specimens in the South-East of Iran. Methods: This cross-sectional study was performed on 1079 cervical fluid cytology specimens referred for two years, between 2018-2020. Polymerase chain reaction (PCR) and hybridization (INNO-LiPA HPV Genotyping EXTRA II assay) were used to determine HPV DNA and their genotypes, respectively. Results: HPV was positive in 37.7% (407 of 1079) patients with a mean age of 34.62 ± 8.82. Among positive cases, 252 (62%) had only one HPV genotype and 155 (38.05%) had multiplex HPV genotypes, which included 94 (60.7%), 38 (24.6%), 18 (11.6%) and 5 (3.2%) cases with two, three, four and five or more genotypes, respectively. The samples with multiple strains revealed 31 HPV genotypes with the four most prevalent being HPV6 (14.7%), HPV16 (10.9%), HPV53 (9.6%) and HPV51 (5.9%). Conclusion: HPV infection is the main health challenge for women that requires improved health service programs and appropriate epidemic vaccination.

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Prevalence and correlation of human papillomavirus genotypes with clinical factors in cervical samples from Mexican women

Experimental Biology and Medicine ◽

10.1177/1535370220959747 ◽

2020 ◽

pp. 153537022095974

Author(s):

Fabiola Hernández-Rosas ◽

Erika Orozco-Hernández ◽

Liliana Maza-Sánchez ◽

Pamela Citlalli Salgado-García ◽

Enrique Navarro-Vidal ◽

...

Keyword(s):

Risk Factors ◽

Cervical Cancer ◽

Cancer Screening ◽

Hpv Infection ◽

Sexual Partners ◽

Mexican Women ◽

Hpv Dna ◽

Screening Strategies ◽

Hpv Genotypes ◽

Cytological Abnormalities

In the last decade, the inclusion of HPV DNA testing in cervical cancer screening has provided one of the best strategies for the prevention and timely detection of HPV. We conducted a high-throughput HPV genotyping study based on MALDI-TOF mass spectrometry to determine the prevalence of 24 HPV genotypes, including oncogenic genotypes, in Mexican women and correlated the results with cytological findings and clinical variables. We likewise identified the risk factors in patients with the HPV infection. Our study included 1000 women from Sonora, Mexico, who participated in cervical cancer screening campaigns and who underwent a Pap smear and HPV DNA test. The results showed that the overall prevalence of HPV was 27.2%, 18.5% with single, and 8.7% multiple infections. The low-risk HPV genotype 6 (8.5%) and oncogenic genotypes 31 (8.1%) and 53 (4.4%) were the most prevalent in the study population. The number of lifetime sexual partners, previous STIs, and age at first intercourse was significantly associated with HPV infection ( P ≤ 0.05). Smoking (OR = 1.5609; 95% IC 1.062–2.292) and more than three lifetime sexual partners (OR = 1.609; 95% IC = 1.124–2.303) represented risk factors for HPV infection. Cytological abnormalities were found in 3.4% of the HPV-positive samples. CIN 1–3 occurred in 0.6% of high-risk HPV cases. In general, the prevalence of the HPV genotypes is high in Mexican women with normal cytological findings. This issue highlights the importance of HPV research in seemingly healthy women and could help guide screening strategies for cervical cancer prevention in Mexico. Impact statement We are submitting data regarding the prevalence and type distribution of the HPV infection and the risk factors associated with it, which may provide a valuable reference to reinforce screening strategies, and to maintain HPV genotype surveillance in Mexico. We discuss the overall prevalence of HPV infection as detected in normal cytological samples stratified by age, different types of infection, and oncogenic capacity. One of the most important findings was that common HPV genotypes detected in healthy women were the genotype numbers: 6, 31, 16, and 56, likewise, smoking and having a history of more than three sexual partners over their lifetime, represented the main risk factors in this study. Furthermore, we found a low frequency of cytological abnormalities and CIN 1–3 in women with HR-HPV.

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Human papillomavirus genotype distribution and risk factors for infection in women from a small municipality in north east Brazil

International Journal of STD & AIDS ◽

10.1258/ijsa.2009.009199 ◽

2012 ◽

Vol 23 (9) ◽

pp. e5-e10 ◽

Cited By ~ 4

Author(s):

F A Oliveira ◽

V Ehrig ◽

K Lang ◽

J Heukelbach ◽

M Stoffler-Meilicke ◽

...

Keyword(s):

Human Papillomavirus ◽

Preventive Measure ◽

Hpv Infection ◽

Population Based ◽

Genotype Distribution ◽

Hpv Dna ◽

Small Municipality ◽

North East ◽

Hpv Genotypes ◽

Cervical Cancer Screening Programme

In order to assess the prevalence of human papillomavirus (HPV) infection, the HPV genotypes and factors associated with infection, we conducted a population-based survey in a small municipality in north east Brazil among women aged between 12 and 49 years. A questionnaire regarding socioeconomic variables, reproductive life and sexual behaviour was used, and women were examined gynaecologically, followed by collection of vaginal lavage with saline solution for HPV DNA determination. HPV DNA was detected by the Digene®SHARP SignalTM-System, and further genotyped by INNO-LiPA Genotyping System®. Of 579 women, HPV infection was present in 68 (prevalence: 11.7%; 95% CI: 9.3–14.7). The most common HPV types were 16, 31 and 74, each accounting for 14.7% of infections. Of all HPV-positive women, 35.3% showed multiple HPV genotypes. Variables independently associated with HPV infection were: ≥3 partners in life (adjusted OR [aOR]: 3.06; 95% CI: 1.68–5.60) and the use of oral contraception in the last 12 months (aOR: 2.39; 95% CI: 1.33–4.30). Previous participation in a cervical cancer screening programme was protective (aOR: 0.28; 95% CI: 0.13–0.60). HPV infection is common among women from rural Brazil, and HPV genotypes identified indicate that immunization could be an important preventive measure in this population.

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Prevalence and distribution of human papillomavirus genotypes among women attending gynecology clinics in northern Henan Province of China

Virology Journal ◽

10.1186/s12985-021-01732-8 ◽

2022 ◽

Vol 19 (1) ◽

Author(s):

Xiangpeng Wang ◽

Yuan Song ◽

Xiaofei Wei ◽

Guanyu Wang ◽

Ruili Sun ◽

...

Keyword(s):

Human Papillomavirus ◽

Hpv Infection ◽

Henan Province ◽

Genotype Distribution ◽

Hpv Dna ◽

Hpv Prevalence ◽

Specific Hybridization ◽

Current Prevalence ◽

Hpv Genotypes ◽

High Risk Hpv

Abstract Background Human papillomavirus (HPV) infection can cause cervical and other cancers, including vulva, vagina, penis, anus, or oropharynx. However, in China's northern Henan Province, data on the prevalence and genotype distribution of HPV among women attending gynecology clinics is limited. This study aimed to investigate the current prevalence and genotype distribution of HPV among women attending gynecology clinics in northern Henan Province. Methods This study included 15,616 women aged 16–81 years old who visited the Xinxiang central hospital's gynecology department between January 2018 and December 2019. HPV DNA was detected by a conventional PCR method followed by HPV type-specific hybridization, which was designed to detect 17 high-risk HPV (HR-HPV) genotypes and 20 low-risk HPV (LR-HPV) genotypes. HPV prevalence and corresponding 95% confidence intervals (95% CI) were calculated using SPSS 18.0. Results The overall HPV prevalence was 19.7% among women in northern Henan Province. Single, double, and multiple HPV infections accounted for 13.7%, 4.3%, and 1.8% of the total cases. Most infections were caused by HR-HPV (71.8%), and single genotype HPV infection (13.7%) was the most common pattern. The most common HR-HPV genotype was HPV16 (4.3%), followed by HPV52 (3.5%) and HPV58 (2.0%). The most common LR-HPV genotype was HPV6 (1.4%), followed by HPV61 (1.1%) and HPV81 (1.1%). Conclusions HPV infection is high among women attending gynecology clinics in northern Henan Province. The highest prevalence was found in women less than 20 years old. In northern Henan Province, the 9-valent HPV vaccine is strongly recommended for regular immunization.

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Circulation of human papillomavirus (HPV) genotypes in women from Córdoba, Argentina, with squamous intraepithelial lesions

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652012000100003 ◽

2012 ◽

Vol 54 (1) ◽

pp. 11-16 ◽

Cited By ~ 3

Author(s):

Raul Fernando Venezuela ◽

Ana Ximena Kiguen ◽

Maria Celia Frutos ◽

Cecilia Gabriela Cuffini

Keyword(s):

Human Papillomavirus ◽

Wide Spectrum ◽

Control Measure ◽

Hpv Infection ◽

Multiple Infections ◽

Warning Sign ◽

Squamous Intraepithelial Lesions ◽

Hpv Dna ◽

Hpv Genotypes ◽

Intraepithelial Lesions

Human papillomavirus (HPV) can induce a wide spectrum of squamous intraepithelial lesions (SIL) of varying severity. The aim of the present study was to establish the frequency of HPV infection and identify the genotypes circulating in women from Córdoba, Argentina, in relation to age and cytology. A total of 186 women, between 18 and 65 years old, with antecedents of SIL, underwent a pelvic examination and had cervical cells collected for cytology and HPV DNA detection. Ninety-six samples (51.6%) were positive for HPV detection, and sixty-three (65.6%) of them showed the presence of at least one HR-HPV. Low- and high-grade SIL showed significant association in patients younger than 35 years of age. We found 18 different genotypes, with a greater presence of HR-HPV. Genotypes 16 and 6 were the most frequent. Seven (7.3%) multiple infections, 85.7% of which had at least one HR-HPV, were detected. The detection of a large number of different HPV genotypes is a warning sign. It is thus necessary to strengthen the monitoring of the circulation of high-risk genotypes, currently less prevalent in intraepithelial lesions, as a control measure for the possible impact of the implementation of vaccines against genotypes 16 and 18.

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