Towards the integration of dark- and photo-fermentative waste treatment. 3. Potato as substrate for sequential dark fermentation and light-driven H2 production

2010 ◽  
Vol 35 (16) ◽  
pp. 8536-8543 ◽  
Author(s):  
Tatyana V. Laurinavichene ◽  
Boris F. Belokopytov ◽  
Kestutis S. Laurinavichius ◽  
Darya N. Tekucheva ◽  
Michael Seibert ◽  
...  
Keyword(s):  
Waste Treatment ◽  
H2 Production ◽  
Dark Fermentation

Significance of acetogenic H2 consumption in dark fermentation and effectiveness of pH

2008 ◽  
Vol 57 (6) ◽  
pp. 809-814 ◽  
Author(s):  
B. Calli ◽  
J. Zhao ◽  
E. Nijssen ◽  
K. Vanbroekhoven
Keyword(s):  
Partial Pressure ◽  
Consumption Rate ◽  
H2 Production ◽  
Dark Fermentation ◽  
Batch Experiments ◽  
Acetate Production ◽  
Butyrate Fermentation ◽  
Reduction Of Co2 ◽  
H2 Yield

Two identical thermophilic H2 fermenters (R1 and R2) were operated at different pH levels between 4.7 and 5.7. In R1, several unexpected and severe drops in H2 yield inversely proportional to increase in acetate production were experienced at pH 5.5 and 5.7. In contrast, R2 operated at pH 5and 4.7 performed more stable H2 production mainly through butyrate fermentation. Although the H2 partial pressure (>50 kPa) was far above the favorable values, acetate was produced as well as butyrate in all pH levels tested. To determine whether some portion of the acetate is produced through another pathway such as autotrophic synthesis via H2 dependent reduction of CO2 or not, batch dissolved H2 consumption rate tests were performed at pH 5.0, 5.5 and 6. The specific H2 consumption rate was 488(±49) μmol/gVSS.hr at pH 6 and slightly higher than at pH 5and 5.5. The results of continuous and batch experiments revealed that acetogenic H2 consumption is more favorable at pH levels above 5.5 and is one of the reasons of instabilities in dark fermentative H2 production.

Engineering strategies for the enhanced photo-H2 production using effluents of dark fermentation processes as substrate

2010 ◽  
Vol 35 (24) ◽  
pp. 13356-13364 ◽  
Author(s):  
Chun-Yen Chen ◽  
Kuei-Ling Yeh ◽  
Yung-Chung Lo ◽  
Hui-Min Wang ◽  
Jo-Shu Chang
Keyword(s):  
H2 Production ◽  
Dark Fermentation ◽  
Fermentation Processes

A framework for integrating functional and microbial data: The case of dark fermentation H2 production

2020 ◽  
Vol 45 (56) ◽  
pp. 31706-31718
Author(s):  
Marcelo Navarro-Díaz ◽  
Mariana Esther Martinez-Sanchez ◽  
Idania Valdez-Vazquez ◽  
Ana E. Escalante
Keyword(s):  
H2 Production ◽  
Dark Fermentation

scholarly journals Improvement of Biohydrogen and Usable Chemical Products from Glycerol by Co-Culture of Enterobacter spH1 and Citrobacter freundii H3 Using Different Supports as Surface Immobilization

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 154
Author(s):  
Biniam T. Maru ◽  
Francisco Lopez ◽  
Francesc Medina ◽  
Magda Constantí
Keyword(s):  
Specific Surface ◽  
Surface Area ◽  
Specific Surface Area ◽  
H2 Production ◽  
Dark Fermentation ◽  
Biodiesel Production ◽  
Citrobacter Freundii ◽  
Surface Immobilization ◽  
Iron Species ◽  
Glycerol Conversion

Glycerol is a by-product of biodiesel production in a yield of about 10% (w/w). The present study aims to improve the dark fermentation of glycerol by surface immobilization of microorganisms on supports. Four different supports were used—maghemite (Fe2O3), activated carbon (AC), silica gel (SiO2), and alumina (γ-Al2O3)—on which a newly isolated co-culture of Enterobacter spH1 and Citrobacter freundii, H3, was immobilized. The effect of iron species on dark fermentation was also studied by impregnation on AC and SiO2. The fermentative metabolites were mainly ethanol, 1,3-propanediol, lactate, H2 and CO2. The production rate (Rmax,i) and product yield (Yi) were elucidated by modeling using the Gompertz equation for the batch dark fermentation kinetics (maximum product formation (Pmax,i): (i) For each of the supports, H2 production (mmol/L) and yield (mol H2/mol glycerol consumed) increased in the following order: FC < γ-Al2O3 < Fe2O3 < SiO2 < Fe/SiO2 < AC < Fe/AC. (ii) Ethanol production (mmol/L) increased in the following order: FC < Fe2O3 < γ-Al2O3 < SiO2 < Fe/SiO2 < Fe/AC < AC, and yield (mol EtOH/mol glycerol consumed) increased in the following order: FC < Fe2O3 < Fe/AC < Fe/SiO2 < SiO2 < AC < γ-Al2O3. (iii) 1,3-propanediol production (mmol/L) and yield (mol 1,3PDO/mol glycerol consumed) increased in the following order: γ-Al2O3 < SiO2 < Fe/SiO2 < AC < Fe2O3 < Fe/AC < FC. (iv) Lactate production(mmol/L) and yield (mol Lactate/mol glycerol consumed) increased in the following order: γ-Al2O3 < SiO2 < AC < Fe/SiO2 < Fe/AC < Fe2O3 < FC. The study shows that in all cases, glycerol conversion was higher when the support assisted culture was used. It is noted that glycerol conversion and H2 production were dependent on the specific surface area of the support. H2 production clearly increased with the Fe2O3, Al2O3, SiO2 and AC supports. H2 production on the iron-impregnated AC and SiO2 supports was higher than on the corresponding bare supports. These results indicate that the support enhances the productivity of H2, perhaps because of specific surface area attachment, biofilm formation of the microorganisms and activation of the hydrogenase enzyme by iron species.

scholarly journals Bio-Hydrogen Production from Wastewater: A Comparative Study of Low Energy Intensive Production Processes

2021 ◽  
Vol 3 (1) ◽  
pp. 156-182
Author(s):  
A K M Khabirul Islam ◽  
Patrick S. M. Dunlop ◽  
Neil J. Hewitt ◽  
Rose Lenihan ◽  
Caterina Brandoni
Keyword(s):  
Wastewater Treatment ◽  
Hydrogen Production ◽  
Scale Up ◽  
Clean Energy ◽  
H2 Production ◽  
Dark Fermentation ◽  
Low Energy ◽  
Synthetic Wastewater ◽  
Hydrogen Yield ◽  
Wastewater Remediation

Billions of litres of wastewater are produced daily from domestic and industrial areas, and whilst wastewater is often perceived as a problem, it has the potential to be viewed as a rich source for resources and energy. Wastewater contains between four and five times more energy than is required to treat it, and is a potential source of bio-hydrogen—a clean energy vector, a feedstock chemical and a fuel, widely recognised to have a role in the decarbonisation of the future energy system. This paper investigates sustainable, low-energy intensive routes for hydrogen production from wastewater, critically analysing five technologies, namely photo-fermentation, dark fermentation, photocatalysis, microbial photo electrochemical processes and microbial electrolysis cells (MECs). The paper compares key parameters influencing H2 production yield, such as pH, temperature and reactor design, summarises the state of the art in each area, and highlights the scale-up technical challenges. In addition to H2 production, these processes can be used for partial wastewater remediation, providing at least 45% reduction in chemical oxygen demand (COD), and are suitable for integration into existing wastewater treatment plants. Key advancements in lab-based research are included, highlighting the potential for each technology to contribute to the development of clean energy. Whilst there have been efforts to scale dark fermentation, electro and photo chemical technologies are still at the early stages of development (Technology Readiness Levels below 4); therefore, pilot plants and demonstrators sited at wastewater treatment facilities are needed to assess commercial viability. As such, a multidisciplinary approach is needed to overcome the current barriers to implementation, integrating expertise in engineering, chemistry and microbiology with the commercial experience of both water and energy sectors. The review concludes by highlighting MECs as a promising technology, due to excellent system modularity, good hydrogen yield (3.6–7.9 L/L/d from synthetic wastewater) and the potential to remove up to 80% COD from influent streams.

scholarly journals Collection of some microbial consortia producing hydrogen from anaerobic wastes

2013 ◽  
Vol 16 (1) ◽  
pp. 51-59
Author(s):  
Hanh Thi Kim Pham ◽  
Anh Thi Ngoc To ◽  
Anh Duong Tam Nguyen
Keyword(s):  
Room Temperature ◽  
Waste Treatment ◽  
Treatment Plant ◽  
H2 Production ◽  
Microbial Consortia ◽  
Hydrogen Yield ◽  
Cultivation Time ◽  
Fermentative Hydrogen Production ◽  
Waste Treatment Plant ◽  
Fermentative Hydrogen

The preparation of hydrogen-producing microbial consortia from three anaerobic digested sludges were carried out by four different pretreatment methods (heat – shock, acid, base and aeration treatment) as well as untreatment. The obtained microbial seeds have been estimated for their stability in fermentative hydrogen production by three consecutive batch fermentations under the same conditions of pH 6.5, room temperature and cultivation time and also investigated the H2 fermentation from different concentrations of glucose and xylose. Three microbial seeds have the most effective H2 production at 5 g/l of glucose or xylose after 48 h cultivation time. The sewage sludge pretreated at 80oC for 30 minutes shows the hydrogen yield of 1.27 mol/mol glucose and 0.82 mol/mol xylose. The sludge in the biogas tank pretreated at 60oC for 30 minutes has the hydrogen yield of 1.27 mol/mol glucose and 0.71 mol/mol xylose. The sludge of the Hoa Binh waste treatment plant pretreated at 60oC for 30 minutes presents the hydrogen yield of 1.31 mol/mol glucose and 0.66 mol/mol xylose.

Apparent hydrogen consumption in acid reactors: observations and implications

2009 ◽  
Vol 59 (7) ◽  
pp. 1441-1447 ◽  
Author(s):  
C. Dinamarca ◽  
R. Bakke
Keyword(s):  
Hydrogen Production ◽  
Biomass Concentration ◽  
H2 Production ◽  
Dark Fermentation ◽  
Operating Conditions ◽  
Fuel Production ◽  
Hydrogen Consumption ◽  
Operation Conditions ◽  
Green Fuel ◽  
High Biomass Concentration

Investigations of hydrogen production by dark fermentation have received increasing attention as a green fuel production process. Research focus is mainly on yields and rates of hydrogen production under different operation conditions. The importance of hydrogen consumption is addressed here, based on results from lab-scale reactors. Experiments were run using mixed cultures and a variety of operating conditions: HRT 6-40 hours; temperature 25–55°C. Initial hydrogen yields between 0.8–1.5 mol H2/mol glucose and ≈50% H2 in headspace was observed, followed by a decrease in hydrogen production as the culture matures, resulting in hydrogen yields down to 0.02 mol H2/mol glucose. It is concluded that hydrogen or “hydrogen equivalents” consumption is significant, especially in reactors with high biomass concentration and/or high sludge age. Sustainable H2 production by dark fermentation alone is therefore not likely to be developed. The results suggest that it is possible to control and avoid significant H2 production in dark fermentation. Minimizing H2 production can be useful in preparation of organic feed for other bio-fuel production processes, such as methanogenic processes and bio-electrochemical H2 production.

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