scholarly journals Molecular analysis and risk factors for nasal colonization with methicillin-resistant Staphylococcus aureus among community individuals attending a Family Health Care Program in Rio de Janeiro, Brazil

2010 ◽  
Vol 14 ◽  
pp. e207
Author(s):  
T. Miranda ◽  
T. Brust ◽  
N.H.T. Miyazaki ◽  
H. Kang ◽  
L. Isaias ◽  
...  
Keyword(s):  
Risk Factors ◽  
Health Care ◽  
Staphylococcus Aureus ◽  
Molecular Analysis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Family Health ◽  
Care Program ◽  
Nasal Colonization ◽  
Methicillin Resistant ◽  
Health Care Program

scholarly journals COMPARISON OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS IN HEALTHY COMMUNITY HOSPITAL VISITORS[CA-MRSA] AND HOSPITAL STAFF [HA-MRSA]

2015 ◽  
Vol 7 ◽  
pp. e2015053 ◽  
Author(s):  
Nirmal A Pathare ◽  
Anil Pathare
Keyword(s):  
Risk Factors ◽  
Health Care ◽  
Staphylococcus Aureus ◽  
Confidence Interval ◽  
Cell Phone ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Community Hospital ◽  
Nasal Colonization ◽  
Methicillin Resistant ◽  
Healthy Community

Background: The prevalence of community associated methicillin resistant Staphylococcus aureus [CA-MRSA] in unknown in Oman.Methods: Nasal and cell phones swabs were collected from hospital visitors and health-care workers on sterile polyester swabs and directly inoculated onto a mannitol salt agar containing oxacillin, allowing growth of methicillin-resistant microorganisms. Antibiotic susceptibility tests were performed using Kirby Bauer’s disc diffusion method on the isolates. A brief survey questionnaire was requested be filled to ascertain the exposure to known risk factors for CA-MRSA carriage.Results: Overall, nasal colonization with CA-MRSA was seen in 34 individuals (18%, 95% confidence interval [CI] =12.5%-23.5%), whereas, CA-MRSA was additionally isolated from the cell phone surface in 12 participants (6.3%, 95% CI =5.6%-6.98%). Nasal colonization prevalence with HA-MRSA was seen in 16 individuals (13.8%, 95% confidence interval [CI] =7.5%-20.06%), whereas, HA-MRSA was additionally isolated from the cell phone surface in 3 participants (2.6%, 95% CI =1.7-4.54).  Antibiotic sensitivity was 100% to linezolid and rifampicin in the CA-MRSA isolates. Antibiotic resistance to vancomycin and clindamycin varied between 9-11 % in the CA-MRSA isolates.  There was no statistically significant correlation between CA-MRSA nasal carriage and the risk factors (P>0.05, Chi-square test).Conclusions: The prevalence of CA-MRSA in the healthy community hospital visitors was 18 % (95% CI, 12.5% to 23.5%) as compared to 13.8% [HA-MRSA] in the hospital health-care staff. In spite of a significant prevalence of CA-MRSA, these strains were mostly sensitive. Recommendation the universal techniques of hand washing, personal hygiene and sanitation are thus warranted. 

scholarly journals Methicillin-resistant Staphylococcus aureus colonization among healthcare workers in Oman

2021 ◽  
Vol 15 (10) ◽  
pp. 1426-1435
Author(s):  
Loay Al Wahaibi ◽  
Rajaa Al Sudairi ◽  
Abdullah Balkhair ◽  
Huda Al-Awaisi ◽  
Mohamed Mabruk
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Healthcare Workers ◽  
Nasal Swab ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Aureus Strain ◽  
Nasal Colonization ◽  
Methicillin Resistant ◽  
Associated Risk Factors ◽  
Mrsa Colonization

Introduction: Methicillin-Resistant Staphylococcus aureus (MRSA) is a S. aureus strain characterized by resistance to cloxacillin. Healthcare workers (HCWs), are recognized for their heightened risk for MRSA acquisition and possibly for MRSA nosocomial transmission. This cross-sectional study aimed to determine the prevalence and the associated risk factors of MRSA colonization among healthcare workers at Sultan Qaboos University Hospital (SQUH) in Oman. Methodology: A total of 200 nasal swab samples were collected from the healthcare workers at SQUH during the period October 2nd 2018 to January 7th 2019. All nasal swab samples were examined microbiologically for the presence of MRSA using the standard method and the results were confirmed by detection of the mecA product (PBP2a). Data on associated risk factors for MRSA colonization was collected and analyzed. Results: Forty-one of the 200 screened healthcare workers (20.5%) were found to have nasal carriage of Staphylococcus aureus of which 63.4% were Methicillin Sensitive and 36.6% were Methicillin-Resistant (MRSA). Methicillin-Resistant Staphylococcus aureus (MRSA) was isolated from fifteen of the 200 screened healthcare workers giving a prevalence rate of nasal colonization with MRSA of 7.5%. We found no statistical association between healthcare worker MRSA nasal colonization and age, gender, HCWs specialty, hand hygiene practices, skin condition, previous MRSA infection, and previous exposure to antibiotics. Conclusions: Identification of the prevalence and the associated risk factors of MRSA colonization in healthcare workers mandates continuous surveillance and the implementation of all possible preventive measures to reduce re-occurrences.

scholarly journals Nasal colonization by methicillin-sensitive and methicillin-resistant Staphylococcus aureus among medical students

2018 ◽  
Vol 12 (05) ◽  
pp. 326-335 ◽  
Author(s):  
Mohammad Al-Tamimi ◽  
Nisreen Himsawi ◽  
Jumana Abu-Raideh ◽  
Deaa Abu jazar ◽  
Hussam Al-jawaldeh ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Medical Students ◽  
Molecular Analysis ◽  
Nosocomial Infections ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Diffusion Method ◽  
Nasal Colonization ◽  
Disc Diffusion ◽  
Methicillin Resistant ◽  
Vitek 2

Introduction: Nasal carriers of methicillin-resistant Staphylococcus aureus (MRSA) are common and play an important role in nosocomial infections. The prevalence rate and characterization of nasal carriers of MRSA among medical students in Jordan has not been investigated before. Methodology: The resistance of S. aureus to several antibiotics was tested using disc diffusion method, automatic Vitek 2, and penicillin binding protein (PBP) 2 slide test. Bacterial species and resistance genes were confirmed using molecular analysis of three relevant genes by real-time PCR. Two hundred ninety nasal swabs were collected from medical students at Hashemite University from June 2015 to August 2016. All participants signed a voluntary consent form and filled a predesigned questionnaire. Results: The mean age of participants was 19.7 ± 2 years and 61.7% of them were males. 63 out of the 290 (21.7%) samples were identified to have S. aureus, 56 (19.3%) were methicillin-sensitive S. aureus (MSSA) and 7 (2.4%) were MRSA. S. aureus nasal colonization significantly associates with male gender (OR = 1.7, CI = 0.94-3.18, P = 0.049) and chronic illnesses (OR = 4.0, CI = 1.52-10.65, P = 0.006). Consistency between disc diffusion, Vitek 2, and PBP 2 methods for MRSA screening were satisfactory compared to molecular analysis. All MRSA samples were positive for SCCmec:orfx junction gene (MRSA-specific), nuc gene (S. aureus- specific), mecA gene (PBP-mediated resistant), and PBP2 production. All MRSA isolates were multi-drug resistant and were sensitive to Linezolid, Vancomycin, and Tigecycline. Conclusions: This study confirms that nasal colonization by MRSA among medical students necessitates further attention to prevent nosocomial infections.

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