scholarly journals Antibiotic Resistance Pattern In Mrsa Isolates In Relation To Meca Gene, Pvl Gene And Its Various Sccmec Types

2021 ◽  
Vol 39 ◽  
pp. S15
Author(s):  
E. Kavitha
Author(s):  
S. N. Ghodasara ◽  
J. H. Purohit ◽  
J. S. Patel ◽  
B. S. Mathapati ◽  
B. B. Javia ◽  
...  

The aim of this study was to evaluate the antibiotic resistance pattern of Methicillin-Resistant Staphylococci (MRS) isolated from animals and human beings. During the study, total 9 MRS isolates from 86 Staphylococcus spp. and 20 MRS isolates from 62 Staphylococcus spp. were identified from 202 animal and 100 human samples, respectively. All the MRS isolates from animals showed higher susceptibility to amikacin and rifampicin (100%) followed by oxytetracyclin (77.78%) and chloramphenicol (66.66%). Similarly, the MRS isolates from human showed higher susceptibility to methicillin (98%) followed by rifampicin and gentamicin (90% each), chloramphenicol (80%) and ofloxacin and levofloxacin (70% each). The higher rates of methicillin, gentamicin, ofloxacin and levofloxacin sensitivity were observed in human beings as compared to animal MRS isolates, whereas higher rates of amikacin, rifampicin and oxytetracyclin sensitivity were observed in animal isolates as compared to human MRS isolates. The MIC level of all the MRS isolates from both the species were recorded and found 89.67% correlation of phenotypic oxacillin susceptibility test with mecA gene PCR amplification among MRS isolates from animal and human.


Author(s):  
N. Momtaz Bokharaei ◽  
M.M. Soltan Dallal ◽  
M.R. Pourmand ◽  
Z. Rajabi

Background: Among the bacteria that cause food poisoning, Staphylococcus aureus is one of the most common causes of food poisoning worldwide. The aim of this study was to investigate the presence of S. aureus strains in Iranian hamburgers, analysis of their antibiotic resistance pattern, and molecular detection of mecA gene in isolated strains. Methods: A total of 100 Iranian handmade (traditional) and packaged (factory-made) hamburger samples were investigated for the existence of S. aureus. The pattern of antibiotic resistance and the presence of mecA genes were investigated by disk diffusion and molecular methods, respectively. Data were statistically analyzed by SPSS software v. 24. Results: The prevalence of S. aureus isolated in handmade hamburgers was significantly (p=0.008) higher than packaged ones. Most of 39 isolated S. aureus strains were susceptible to ciprofloxacin (31 isolate), chloramphenicol (27 isolate), and trimethoprim/sulfamethoxazole (37 isolate). The highest antibiotic resistance was observed for penicillin G followed by oxacillin and tetracycline. All isolates were found susceptible to vancomycin and gentamicin. Six S. aureus isolates which were evaluated for methicillin-resistance, contained the mecA gene. Conclusion: The high presence of the S. aureus in Iranian hamburgers and the remarkable antibiotic resistance emphasize the need for policies which enforce hygienic practices within the food industry and fast food outlets.


Gene Reports ◽  
2021 ◽  
pp. 101195
Author(s):  
Hossein Masoumi-Asl ◽  
Fatemah Sadeghpour Heravi ◽  
Ali Badamchi ◽  
Khadijeh Khanaliha ◽  
Marziye Farsimadan ◽  
...  

2018 ◽  
Vol 7 (1) ◽  
pp. 28-40
Author(s):  
Bikram Gautam ◽  
Rameshwar Adhikari

Wastewater treatment plant is a potential reservoir contributing to the evolution and spread of heavy metal and antibiotic resistant bacteria. The pollutants such as biocides, antibiotics, heavy metals are to be feared for as they have been known to evoke resistance in microorganisms in such polluted environment. The aim of this study was to the isolate bacteria from the treated wastewater and assess the resistance pattern of the isolates against antibiotics and heavy metals. Grab sampling was performed from April to June 2017, from the treated effluent from the secondary treatment plant. To assess the resistance pattern for antibiotic(s) and heavy metal(s), antibiotic susceptibility test and minimum inhibitory concentration by cup well method were performed respectively. Staphylococcus aureus, Enterococcus faecalis, Citrobacter freundii, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Salmonella Typhi, Pseudomonas aeruginosa were isolated. Multi drug and heavy metal resistant isolates were screened. Fisher’s exact test revealed that there is a significant association (p< 0.001) between antibiotic resistance pattern and resistance patterns at dilution of 2500 g/L (25%). Cramer’s V test revealed that the effect size of antibiotic resistance pattern and heavy metal resistance pattern at dilution 2500 g/L is medium. P. aeruginosa was able to resist the metal concentration up to 10000 g/L (100%) dilution of Fe++. Heavy metal resistant bacteria can be safely used to lower chemical concentration in the environment once their harmful genes are edited, knocked etc. so that risks of evoking antibiotic resistance could be minimized. 


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