Antimicrobial properties of cyclodextrin–antiseptics-complexes determined by microplate laser nephelometry and ATP bioluminescence assay

ABSTRACTTo guide the timely selection of antibiotic combinations against carbapenem-resistant Gram-negative bacteria (CR-GNB), anin vitrotest with a short turnaround time is essential. We developed anin vitroATP bioluminescence assay to determine effective antibiotic combinations against CR-GNB within 6 h. We tested 42 clinical CR-GNB strains (14Acinetobacter baumannii, 14Pseudomonas aeruginosa, and 14Klebsiella pneumoniaestrains) against 74 single antibiotics and two-antibiotic combinations. Bacteria (approximately 5 log10CFU/ml) were incubated with an antibiotic(s) at 35°C; ATP bioluminescence was measured at 6 h and 24 h; and the measurements were compared to viable counts at 24 h. Receiver operating characteristic (ROC) curves were used to determine the optimal luminescence thresholds (TRLU) for distinguishing between inhibitory and noninhibitory combinations. The areas under the 6-h and 24-h ROC curves were compared using the DeLong method. Prospective validation of the established thresholds was conducted using 18 additional CR-GNB. The predictive accuracy ofTRLUfor the 6-h ATP bioluminescence assay was 77.5% when all species were analyzed collectively. Predictive accuracies ranged from 73.7% to 82.7% when each species was analyzed individually. Upon comparison of the areas under the 6-h and 24-h ROC curves, the 6-h assay performed significantly better than the 24-h assay (P< 0.01). Predictive accuracy remained high upon prospective validation of the 6-h ATP assay (predictive accuracy, 79.8%; 95% confidence interval [CI], 77.6 to 81.9%), confirming the external validity of the assay. Our findings indicate that our 6-h ATP bioluminescence assay can provide guidance for prospective selection of antibiotic combinations against CR-GNB in a timely manner and may be useful in the management of CR-GNB infections.

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Study on the Detection of Total Colony in Medical and Health Environment Based on ATP Bioluminescence

Journal of Commercial Biotechnology ◽

10.5912/jcb839 ◽

2018 ◽

Vol 24 (2) ◽

Author(s):

Zhe Li

Keyword(s):

Culture Method ◽

Pass Rate ◽

Plate Count ◽

Count Method ◽

Atp Bioluminescence ◽

Bioluminescence Assay ◽

Plate Count Method ◽

The Difference ◽

Atp Bioluminescence Assay ◽

Bioluminescence Method

In this paper, the application of ATP fluorescence in the detection of colonies in the health environment of hospitals was studied. Firstly, the principle of ATP bioluminescence method was described. Then, ATP bioluminescence and plate count method were used to test the density of the surface of the objects in selected area, taking the time points 2 hours after disinfection as the time nodes. The results showed that the difference between the qualified rate of ATP bioluminescence assay and the plate count method was statistically significant {P<0.01}. Therefore, ATP bioluminescence method was highly correlated with bacterial culture method. The correlation coefficient of pass rate of the two methods was 0.782, which indicated that there was a positive correlation between the two test results. Besides, the detection results showed that ATP bioluminescence method had higher sensitivity than plate counting method. Therefore, ATP bioluminescence method was more suitable for the rapid detection of the colony of hospital health environment, and helps the hospital to better manage its environmental hygiene conditions.

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A case study: Utilization of ATP bioluminescence assay in monitoring medical device cleaning process, environment and hand hygiene in CSSD

Infection Disease & Health ◽

10.1016/j.idh.2017.09.006 ◽

2017 ◽

Vol 22 ◽

pp. S2

Author(s):

Roel Bletran Castillo

Keyword(s):

Hand Hygiene ◽

Medical Device ◽

Cleaning Process ◽

Atp Bioluminescence ◽

Bioluminescence Assay ◽

Atp Bioluminescence Assay

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