Geographic variation in water-soluble polysaccharide content and antioxidant activities of Cyclocarya paliurus leaves

2018 ◽  
Vol 121 ◽  
pp. 180-186 ◽  
Author(s):  
Yang Liu ◽  
Shengzuo Fang ◽  
Mingming Zhou ◽  
Xulan Shang ◽  
Wanxia Yang ◽  
...  
2010 ◽  
Vol 119 (4) ◽  
pp. 1626-1632 ◽  
Author(s):  
Jian-Hua Xie ◽  
Ming-Yong Xie ◽  
Shao-Ping Nie ◽  
Ming-Yue Shen ◽  
Yuan-Xing Wang ◽  
...  

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11615
Author(s):  
Weida Lin ◽  
Huanwei Chen ◽  
Jianmei Wang ◽  
Yongli Zheng ◽  
Qiuwei Lu ◽  
...  

Background Cyclocarya paliurus (Batal.) Iljinskaja is a common endemic tree species and used as a Chinese medicine. The main active components in the leaves of this plant are polysaccharides. However, the temporal patterns of gene expression underlying the synthesis of polysaccharides in C. paliurus at different leaf developmental stages and its relationship with the polysaccharide content and antioxidant activities has not been reported to date. Methods RNA-seq was used to investigate the biosynthesis pathway of polysaccharides at the four developmental stages of C. paliurus leaves. The content and the antioxidant activities of polysaccharides were measured with typical biochemical methods and the identified correlations were statistically evaluated. Results Sixty-nine differentially expressed genes were found in the leaves during different developmental stages of C. paliurus. These are associated with glycosyltransferases and belong to 18 families. During different developmental stages of C. paliurus, the polysaccharide content first increased and then decreased, and the UDP-glucose 4-epimerase gene was found to be significantly positively correlated with the polysaccharide content. The clearance rates of DPPH radicals, superoxide anion radicals, hydroxyl radicals, and the reducing power of polysaccharides in the leaves of C. paliurus at different developmental stages showed a dose-dependent relationship with the concentration of polysaccharides. Conclusions The smallest fully expanded leaves are suitable for high-quality tea, and leaves with sizes below the largest fully expanded leaves are suitable for industrial production of polysaccharides.


2020 ◽  
Vol 144 ◽  
pp. 751-759 ◽  
Author(s):  
Paiheerding Mutaillifu ◽  
Khayrulla Bobakulov ◽  
Aytursun Abuduwaili ◽  
Haibaier Huojiaaihemaiti ◽  
Rehebati Nuerxiati ◽  
...  

2018 ◽  
Vol 18 (1) ◽  
pp. 21-33
Author(s):  
Chen Nai-dong ◽  
Chen Yue ◽  
Zhang Li ◽  
Chen Cun-Wu ◽  
Deng Hui ◽  
...  

The objective of this study was to develop a multi-steps infrared macro-fingerprint method to discriminate Dendrobium huoshanense “Fengdou” (DHS FD) produced under different toasting and soaking conditions as well as to examine the effect of production conditions on the total polyphenol content, total water soluble polysaccharide content, and antioxidant activities. The results showed that though the Fourier transform infrared and second derivative infrared (SD-IR) spectra of the DHS FD processed under different conditions were similar visually, the DHS FDs could be discriminated by their characteristic peaks in the range of 1800–600 cm–1 from their IR and SD-IR files and the cross-peaks in the region of 1280–950 cm–1 in their two-dimensional correlation spectroscopy IR spectra. The total polyphenol content increased and the total water soluble polysaccharide content decreased in the DHS FDs toasted at temperatures from 110°C to 190°C for 30 min. Soaking process showed variable effects on the total polyphenol content and total water soluble polysaccharide content. The total polyphenol content of the DHS FDs toasted below 150°C increased as the soaking temperatures increased while the total water soluble polysaccharide content showed an opposite trend. Both the total polyphenol content and total water soluble polysaccharide content of the DHS FDs toasted at 170°C and 190°C showed little difference when soaking temperatures varied from 4°C to 100°C. The investigation of the 2,2-Diphenyl-1-picrylhydrazyl cleaving activity revealed that toasting and soaking process might increase the antioxidant activity of the DHS while too high toasting temperatures would decrease their activity. The multi-steps infrared macro-fingerprint analysis with high resolution and excellent macroscopic fingerprint features could effectively discriminate the DHS produced under different conditions without involving any separation and extraction. Consequently, the use of this method is recommended for quality control of the DHS FD.


2021 ◽  
Author(s):  
Sirine Ben Slima ◽  
Naourez Ktari ◽  
Imen Trabelsi ◽  
Aicha Chouikhi ◽  
Amina Hzami ◽  
...  

Abstract A novel heteropolysaccharide, named Cress Water Soluble Polysaccharide (CWSP) was purified from Lepidium sativum seeds. Antioxidant activities and functional properties were characterized thermally using thermal gravimetric analysis (TGA), and the differential scanning calorimeter (DSC) of CWSP were evaluated. The total antioxidant capacity and the metal chelating activities of CWSP at 3 mg/ml was equivalent to 116.34 µg acsorbic acid and 62.57%, respectively. CWSP were thermally stable and presented high water (WHC) and oil holding (OHC) capacities and good emulsion properties. CWSP was used for the production of cakes. The formulations samples were prepared with different levels of CWSP (0.1; 0.3 and 0.5%) and analyzed during 15 days of storage at room temperature. The obtained results indicated that the addition of CWSP had a significant effect on the texture profile, leading to the increase in all parameters in terms of hardness, springiness, cohesiveness, adhesiveness and chewiness. Moreover, the reformulation samples presented higher a* and lower L* and b* than control sample. The sensory evaluation showed that the formulation of cake with 0.3% of CWSP was the most acceptable. Therefore, CWSP proved to be a new alternative for improving the quality attributes and showed potent antioxidant activities on the shelf life during the storage of bakery foods.


PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6492 ◽  
Author(s):  
Mingming Zhou ◽  
Yuan Lin ◽  
Shengzuo Fang ◽  
Yang Liu ◽  
Xulan Shang

Cyclocarya paliurus (Batal) Iljinskaja is a multiple function tree species, but its main utilization is for the harvesting of its leaves as materials for tea production and recently as ingredients for the food industry. In this study aqueous extracts of C. paliurus leaves collected from 21 natural populations were evaluated for their phytochemical content and antioxidant activity. The content of water-soluble polysaccharide, total flavonoid and total polyphenol varied from 66.05 to 153.32 mg/g, 9.01 to 19.65 mg/g and 20.80 to 52.69 mg/g, respectively. Quercetin-3-O-glucuronide, aemferol-3-O-glucuronide and 3-O-caffeoylquinic acid were the major phenolic components in aqueous extracts of C. paliurus leaves. Both redundancy analysis and Pearson's correlation analysis indicated that water-soluble polysaccharide, total polyphenol, total flavonoid, quercetin-3-O-glucuronide, 3-O-caffeoylquinic acid and 4-O-caffeoylquinic acid were significantly correlated with antioxidant activity, but total polyphenol showed the greatest contribution to antioxidant capacity. The antioxidant activity of the 21 populations was classified into six distinct groups based on the squared Euclidean distance. These results would provide a theoretical basis for obtaining the greatest yield of targeted antioxidant phytochemicals of C. paliurus leaves for tea and food ingredient production.


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