Simultaneous determination of naphtodianthrones, emodin, skyrin and new bisanthrones in Hypericum perforatum L. in vitro shoot cultures

The aim of our study was to investigate the presence and quantitative contents of lignans in the tissues of Taxus ×media. The presence of the lignans: pinoresinol, matairesinol and secoisolariciresinol was assessed in needles, shoots cultures and suspension culture. Pinoresinol was the only lignan found in the tissue of T. ×media. The total pinoresinol content in the needles and in the shoots was 1.24 mg/g dry weight (dw) and 0.69 mg/g dw, respectively. Most of the pinoresinol identified was appeared glycosidically bound. In needles, the amount of glycosidically bound pinoresinol (0.81 mg/g dw) was about twice as high as that of free pinoresinol (0.43 mg/g dw). The content of free and glycosidically bound pinoresinol showed the level of 0.18 mg/g dw and 0.51 mg/g dw, respectively in the in vitro shoot cultures. In the cell culture, no pinoresinol was found.

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Simultaneous Determination of the Predominant Hyperforins and Hypericins in St. John's Wort (Hypericum perforatum L.) by Liquid Chromatography

Journal of AOAC International ◽

10.1093/jaoac/83.4.944 ◽

2000 ◽

Vol 83 (4) ◽

pp. 944-949 ◽

Cited By ~ 25

Author(s):

Dean E Gray ◽

George E Rottinghaus ◽

H E Gene Garrett ◽

Stephen G Pallardy

Keyword(s):

Liquid Chromatography ◽

Simultaneous Determination ◽

Hypericum Perforatum ◽

Dry Weight ◽

Active Constituents ◽

St John’S Wort ◽

St John's Wort ◽

Relative Standard ◽

Hypericum Perforatum L

Abstract Hypericin and hyperforin are believed to be among the active constituents in common St. John's wort (Hypericum perforatum L.). Presently, dietary supplements are generally standardized to contain specified levels of hypericin and hyperforin, and the related compounds, pseudohypericin and adhyperforin. A rapid method was developed for simultaneous determination of these 4 active constituents by liquid chromatography (LC). A 1 g portion of dried, finely ground leaf/flower sample is extracted with 20 mL methanol for 2 h. A 0.6 mL aliquot of the crude extract is combined with 5.4 mL acetonitrile–methanol (9 + 1) and passed through a mixed solid-phase cleanup column. The eluate is examined by LC for hyperforin, adhyperforin, hypericin, and pseudohypericin on a Hypersil reversed-phase column by using simultaneous ultraviolet (284 nm) and fluorescence detection (excitation, 470 nm; emission, 590 nm). The compounds are easily separated isocratically within 8 min with a mobile phase of acetonitrile–aqueous 0.1M triethylammonium acetate (8 + 2). Average recoveries of hyperforin and adhyperforin were 101.9 and 98.4%, respectively, for 3 sample mixtures containing concentrations ranging from approximately 0.2 to 1.5% combined hyperforins per gram dry weight. Average relative standard deviation (RSD) values for hyperforin and adhyperforin for all 3 mixtures were 18.9 and 18.0%, respectively. Average recoveries of hypericin and pseudohypericin were 88.6 and 93.3% respectively, from 3 sample mixtures containing concentrations ranging from approximately 0.2 to 0.4% combined hypericins per gram dry weight. Average RSD values for hypericin and pseudohypericin for all 3 mixtures were 3.8 and 4.2%, respectively.

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Determination of Hypericin in St. John's Wort (Hypericum perforatum L.) extracts using HPLC-ED

Planta Medica ◽

10.1055/s-0031-1282174 ◽

2011 ◽

Vol 77 (12) ◽

Author(s):

E Sofic ◽

A Copra Janicijevic ◽

M Maksimovic ◽

I Tahirovic ◽

L Klepo ◽

...

Keyword(s):

Hypericum Perforatum ◽

St John’S Wort ◽

St John's Wort ◽

Hypericum Perforatum L

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Simultaneous determination of eight derivatives of propranolol in cornea perfusate in vitro by high performance liquid chromatography

Chinese Journal of Chromatography ◽

10.3724/sp.j.1123.2012.06039 ◽

2013 ◽

Vol 30 (11) ◽

pp. 1183-1187

Author(s):

Haitao WU ◽

Chuanbing CHEN ◽

Ningsheng WANG ◽

Suiqing MI ◽

Nanying LIAO

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Simultaneous Determination ◽

High Performance ◽

Derivatives Of

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Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions

Plants ◽

10.3390/plants10040670 ◽

2021 ◽

Vol 10 (4) ◽

pp. 670

Author(s):

Katalin Magyar-Tábori ◽

Nóra Mendler-Drienyovszki ◽

Alexandra Hanász ◽

László Zsombik ◽

Judit Dobránszki

Keyword(s):

Adverse Effects ◽

Physiological Condition ◽

Harmful Effect ◽

Shoot Cultures ◽

Virus Elimination ◽

In Vitro Shoots ◽

Further Development ◽

In Vitro Shoot Cultures ◽

Harmful Effects

In general, in vitro virus elimination is based on the culture of isolated meristem, and in addition thermotherapy, chemotherapy, electrotherapy, and cryotherapy can also be applied. During these processes, plantlets suffer several stresses, which can result in low rate of survival, inhibited growth, incomplete development, or abnormal morphology. Even though the in vitro cultures survive the treatment, further development can be inhibited; thus, regeneration capacity of treated in vitro shoots or explants play also an important role in successful virus elimination. Sensitivity of genotypes to treatments is very different, and the rate of destruction largely depends on the physiological condition of plants as well. Exposure time of treatments affects the rate of damage in almost every therapy. Other factors such as temperature, illumination (thermotherapy), type and concentration of applied chemicals (chemo- and cryotherapy), and electric current intensity (electrotherapy) also may have a great impact on the rate of damage. However, there are several ways to decrease the harmful effect of treatments. This review summarizes the harmful effects of virus elimination treatments applied on tissue cultures reported in the literature. The aim of this review is to expound the solutions that can be used to mitigate phytotoxic and other adverse effects in practice.

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