Panax notoginseng snakin gene increases resistance to Fusarium solani in transgenic tobacco

2020 ◽  
Vol 157 ◽  
pp. 112902
Author(s):  
Bingling Qiu ◽  
Yingpeng Zhang ◽  
Qian Wang ◽  
Zie Wang ◽  
Hongjun Chen ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Fusarium Solani ◽  
Panax Notoginseng

scholarly journals A methyl jasmonate induced defensin like protein from Panax notoginseng confers resistance against Fusarium solani in transgenic tobacco

2019 ◽  
Vol 63 ◽  
pp. 797-807 ◽  
Author(s):  
Q. WANG ◽  
B.L. QIU ◽  
S. LI ◽  
Y.P. ZHANG ◽  
X.M. CUI ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Transgenic Tobacco ◽  
Fusarium Solani ◽  
Panax Notoginseng

A MYB Transcription Factor Modulates Panax notoginseng Resistance against the Root Rot Pathogen Fusarium solani by Regulating the Jasmonate Acid Signaling Pathway and Photosynthesis

2021 ◽  
Author(s):  
Diqiu Liu ◽  
Bingling Qiu ◽  
Hongjun Chen ◽  
Lilei Zheng ◽  
Linlin Su ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Disease Resistance ◽  
Transgenic Tobacco ◽  
Fusarium Solani ◽  
Root Rot ◽  
Panax Notoginseng ◽  
Defense Responses ◽  
Myb Transcription Factor ◽  
In The Wild ◽  
Pathway Analyses

Root rot of Panax notoginseng, a precious Chinese medicinal plant, seriously impacts its sustainable production. However, the molecular regulatory mechanisms employed by P. notoginseng against root rot pathogens, including Fusarium solani, are still unclear. In this study, the PnMYB2 gene was isolated, and its expression was affected by independent treatments with four signaling molecules, methyl jasmonate, ethephon, salicylic acid, and hydrogen peroxide, as assessed by quantitative real-time PCR. Moreover, the PnMYB2 expression level was induced by F. solani infection. The PnMYB2 protein localized to the nucleus and may function as a transcription factor. When overexpressed in transgenic tobacco, the PnMYB2 gene conferred resistance to F. solani. Jasmonic acid (JA) metabolism and disease resistance-related genes were induced in the transgenic tobacco, and the JA content significantly increased compared with in the wild type. Additionally, transcriptome sequencing, kyoto encyclopedia of genes and genomes（KEGG）annotation enrichment, and metabolic pathway analyses of the differentially expressed genes in the transgenic tobacco revealed that JA metabolic, photosynthetic, and defense response-related pathways were activated. In summary, PnMYB2 is an important transcription factor in the defense responses of P. notoginseng against root rot pathogens that acts by regulating JA signaling, photosynthesis, and disease-resistance genes.

A β‐1,3‐glucanase gene from Panax notoginseng confers resistance in tobacco to Fusarium solani

2020 ◽  
Vol 143 ◽  
pp. 111947 ◽  
Author(s):  
Shah Taif ◽  
Qin Zhao ◽  
Limei Pu ◽  
Xin Li ◽  
Diqiu Liu ◽  
...  
Keyword(s):  
Fusarium Solani ◽  
Panax Notoginseng

scholarly journals Monitoring Antifungal Agents of Artemisia annua against Fusarium oxysporum and Fusarium solani, Associated with Panax notoginseng Root-Rot Disease

Molecules ◽  
2019 ◽  
Vol 24 (1) ◽  
pp. 213 ◽  
Author(s):  
Yu-Nan Ma ◽  
Chuan-Jiao Chen ◽  
Qing-Qing Li ◽  
Fu-Rong Xu ◽  
Yong-Xian Cheng ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Solani ◽  
Root Rot ◽  
Artemisia Annua ◽  
Synergistic Effects ◽  
Petroleum Ether Extract ◽  
Panax Notoginseng ◽  
Root Rot Disease ◽  
Rot Disease

Root rot of Panax notoginseng has received great attention due to its threat on the plantation and sustainable utilization of P. notoginseng. To suppress the root-rot disease, natural ingredients are of great importance because of their environment friendly properties. In this study, we found that the methanol extract from Artemisia annua leaves has strong antifungal effects on the growth of Fusarium oxysporum and Fusarium solani resulting into root-rot disease. Essential oil (EO) thereof was found to be the most active. GC-MS analysis revealed 58 ingredients and camphor, camphene, β-caryophyllene, and germacrene D were identified as the major ingredients. Further antifungal assays showed that the main compounds exhibit various degrees of inhibition against all the fungi tested. In addition, synergistic effects between A. annua EO and chemical fungicides were examined. Finally, in vivo experiments were conducted and disclosed that P. notoginseng root rot could be largely inhibited by the petroleum ether extract from A. annua, indicating that A. annua could be a good source for controlling P. notoginseng root-rot.

A transcriptome analysis uncovers Panax notoginseng resistance to Fusarium solani induced by methyl jasmonate

2019 ◽  
Vol 41 (12) ◽  
pp. 1383-1396 ◽  
Author(s):  
Diqiu Liu ◽  
Qin Zhao ◽  
Xiuming Cui ◽  
Rui Chen ◽  
Xin Li ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Transcriptome Analysis ◽  
Fusarium Solani ◽  
Panax Notoginseng

scholarly journals Osmotin-Like Protein Gene from Panax notoginseng Is Regulated by Jasmonic Acid and Involved in Defense Responses to Fusarium solani

2020 ◽  
Vol 110 (8) ◽  
pp. 1419-1427 ◽  
Author(s):  
Qin Zhao ◽  
Bingling Qiu ◽  
Shan Li ◽  
Yingpeng Zhang ◽  
Xiuming Cui ◽  
...  
Keyword(s):  
Fusarium Solani ◽  
Panax Notoginseng ◽  
Defense Responses ◽  
Expression Level ◽  
Pcr Analysis ◽  
Disease Resistance Gene ◽  
Reverse Transcription Pcr ◽  
Prokaryotic Expression Vector ◽  
Rapid Amplification

Osmotin and osmotin-like proteins (OLPs) play important roles in plant defense responses. The full-length cDNA sequence of an OLP gene was cloned from Panax notoginseng using rapid amplification of cDNA-end technology and named PnOLP1. A quantitative reverse transcription-PCR analysis showed that the signaling molecules methyl jasmonate, salicylic acid, ethylene, and hydrogen peroxide induced PnOLP1 expression to different degrees. In addition, the expression level of PnOLP1 rapidly increased within 48 h of inoculating P. notoginseng with the root rot pathogen Fusarium solani. Subcellular localization revealed that PnOLP1 localized to the cell wall. A prokaryotic expression vector containing PnOLP1 was constructed and transformed into Escherichia coli BL21 (DE3), and in vitro antifungal assays were performed using the purified recombinant PnOLP1 protein. The recombinant PnOLP1 protein had strong inhibitory effects on the mycelial growth of F. oxysporum, F. graminearum, and F. solani. A plant PnOLP1-overexpression vector was constructed and transfected into tobacco, and the resistance of T2 transgenic tobacco against F. solani was significantly enhanced compared with wild-type tobacco. Moreover, a PnOLP1 RNAi vector was constructed and transferred to the P. notoginseng leaves for transient expression, and the decrease of PnOLP1 expression level in P. notoginseng leaves increased the susceptibility to F. solani. Thus, PnOLP1 is an important disease resistance gene involved in the defense responses of P. notoginseng to F. solani.

Co-expression of AGPS and AGPL genes encoded for AGPase from cassava to enhance starch accumulation in transgenic tobacco

2016 ◽  
Vol 14 (2) ◽  
pp. 287-293
Author(s):  
Nguyễn Văn Đoài ◽  
Nguyễn Minh Hồng ◽  
Lê Thu Ngọc ◽  
Nguyễn Thị Thơm ◽  
Nguyễn Đình Trọng ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Higher Plants ◽  
Starch Content ◽  
Genetically Modified Crops ◽  
Starch Accumulation ◽  
35S Promoter ◽  
Effective Strategies ◽  
Plant Expression Vector ◽  
Transgenic Lines ◽  
Cassava Varieties

The AGPase (ADP-Glucose pyrophosphorylase) is one of the ubiquitous enzymes catalyzing the first step in starch biosynthesis. It plays an important role in regulation and adjusts the speed of the entire cycle of glycogen biosynthesis in bacteria and starch in plants. In higher plants, it is a heterotetramer and tetrameric enzyme consisting two large subunits (AGPL) and two small subunits (AGPS) and encoded by two genes. In this paper, both AGPS and AGPL genes were sucessfully isolated from cassava varieties KM140 and deposited in Genbank with accession numbers KU243124 (AGPS) and KU243122 (AGPL), these two genes were fused with P2a and inserted into plant expression vector pBI121 under the control of 35S promoter. The efficient of this construct was tested in transgenic N. tabacum. The presence and expression of AGPS and AGPL in transgenic plants were confirmed by PCR and Western hybridization. The starch content was quantified by the Anthrone method. Transgenic plant analysis indicated that that two targeted genes were expressed simultaneously in several transgenic tobacco lines under the control of CaMV 35S promoter.  The starch contents in 4 analyzed tobacco transgenic lines displays the increase 13-116%  compared to WT plants. These results indicated that the co-expression of AGPS and AGPL is one of effective strategies for enhanced starch production in plant. These results can provide a foundation for developing other genetically modified crops to increase starch accumulation capacity.

Identificación de aislamientos clínicos de Fusarium spp mediante técnicas moleculares en Colombia.

2014 ◽  
Vol 12 (1) ◽  
pp. 143 ◽  
Author(s):  
Yesid Fabián Acevedo-Granados ◽  
Luz Elena Cano ◽  
Adelaida María Gaviria Rivera
Keyword(s):  
Fusarium Solani ◽  
Fusarium Spp

Fusariumes un género fúngico amplio y diverso de diferentes complejos deespecies, causante de una gran variedad de enfermedades en plantas, productor dediversas toxinas y representa un importante patógeno oportunista en humanos. Laidentificación de las especies de Fusarium ha sido por mucho tiempo una tareacompleja y controversial. Esto es debido principalmente a la aplicación de diferentessistemas taxonómicos y la inherente variabilidad morfológica de algunas de estasespecies. Estas características requieren de la revisión por parte de un expertomicólogo, con el fin de lograr un acertado y confiable diagnóstico, el cual es crucialen el manejo de enfermedades o infecciones y estudios de diversidad genética. EnColombia, se ha reportado un incremento anual del 317 % de casos de infeccionescausadas por Fusarium, entre 1995 y 2003, sin embargo en centros especializados anivel nacional en micología médica, no se lleva a cabo un diagnóstico a nivel deespecie. El objetivo de este estudio fue el de establecer la identidad de aislamientosclínicos de Fusarium, mediante el uso de un marcador molecular. Para lograr esteobjetivo se llevó a cabo la identificación de los 59 aislamientos mediante consulta enla base de datos Fusarium-ID con base en secuencias codificantes del factor deelongación de la traducción EF-1a. Los resultados obtenidos permitieron observar laagrupación de los 59 aislamientos en tres complejos de especies: Fusariumoxysporum(FOSC), Fusarium solani (FSSC) y Fusarium incarnanatum-equiseti(FIESC). Basado en los resultados, se observa que el uso de las secuenciascodificantes para el factor de elongación de traducción permiten una confiableclasificación de los aislamientos de origen clínico y permite ratificar la utilidad queposee este marcador molecular en los distintos complejos de Fusarium.

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